BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0100.p (181 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311811|ref|YP_003775903.1| membrane protein [Herbaspirillu... 128 3e-31 >gi|300311811|ref|YP_003775903.1| membrane protein [Herbaspirillum seropedicae SmR1] Length = 180 Score = 128 bits (321), Expect = 3e-31, Method: Compositional matrix adjust. Identities = 62/63 (98%), Positives = 62/63 (98%) Query: 1 MSTIIAARFELQEDASRAVEALVGAGYPRERISSFFVNPAGQHDRFALGGDRDKSPGAED 60 MSTIIAARFELQEDASRAVEALV AGYPRERISSFFVNPAGQHDRFALGGDRDKSPGAED Sbjct: 1 MSTIIAARFELQEDASRAVEALVAAGYPRERISSFFVNPAGQHDRFALGGDRDKSPGAED 60 Query: 61 TDR 63 TDR Sbjct: 61 TDR 63 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.324 0.132 0.461 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 472,805 Number of extensions: 14441 Number of successful extensions: 70 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 70 Number of HSP's successfully gapped: 1 Length of query: 181 Length of database: 1,619,870 Length adjustment: 79 Effective length of query: 102 Effective length of database: 1,245,805 Effective search space: 127072110 Effective search space used: 127072110 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 127 (53.5 bits)