BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0099.p (570 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311812|ref|YP_003775904.1| glucose-6-phosphate isomerase [... 1182 0.0 gi|300310432|ref|YP_003774524.1| glucose-6-phosphate isomerase [... 568 e-163 >gi|300311812|ref|YP_003775904.1| glucose-6-phosphate isomerase [Herbaspirillum seropedicae SmR1] Length = 570 Score = 1182 bits (3057), Expect = 0.0, Method: Compositional matrix adjust. Identities = 569/570 (99%), Positives = 569/570 (99%) Query: 1 MTTLPAADLQLPLFVYAQRESLTGKSAWRSLQEHYRDRARHFQLARLFAADDKRGESLTA 60 MTTLPAADLQLPLFVYAQRESLTGKSAWRSLQEHYRDRARHFQLARLFAADDKRGESLTA Sbjct: 1 MTTLPAADLQLPLFVYAQRESLTGKSAWRSLQEHYRDRARHFQLARLFAADDKRGESLTA 60 Query: 61 EAVGIYLDYSKNLVTSETMALLMQLARQCGLPDRIEAMFRGDRINLTENRSVLHTALRKP 120 EAVGIYLDYSKNLVTSETMALLMQLARQCGLPDRIEAMFRGDRINLTENRSVLHTALRKP Sbjct: 61 EAVGIYLDYSKNLVTSETMALLMQLARQCGLPDRIEAMFRGDRINLTENRSVLHTALRKP 120 Query: 121 ASERLVVDDQDVVAEVQQVLRRMEGFARQVRSGQWLGHTGLPIRHVVNIGIGGSDLGPMM 180 ASERLVVD QDVVAEVQQVLRRMEGFARQVRSGQWLGHTGLPIRHVVNIGIGGSDLGPMM Sbjct: 121 ASERLVVDGQDVVAEVQQVLRRMEGFARQVRSGQWLGHTGLPIRHVVNIGIGGSDLGPMM 180 Query: 181 AYEALRPYSHDDLTLHFVSDVDGAAMLDTVRGLPAEQTLFIVCSKTFTTLETITNATAAR 240 AYEALRPYSHDDLTLHFVSDVDGAAMLDTVRGLPAEQTLFIVCSKTFTTLETITNATAAR Sbjct: 181 AYEALRPYSHDDLTLHFVSDVDGAAMLDTVRGLPAEQTLFIVCSKTFTTLETITNATAAR 240 Query: 241 AWLVQQLGAEEAVARHFVGVTTNIEEAQRFGIDADNMFGFWDWVGGRYSMTSAIGLSTMV 300 AWLVQQLGAEEAVARHFVGVTTNIEEAQRFGIDADNMFGFWDWVGGRYSMTSAIGLSTMV Sbjct: 241 AWLVQQLGAEEAVARHFVGVTTNIEEAQRFGIDADNMFGFWDWVGGRYSMTSAIGLSTMV 300 Query: 301 AIGPEHFRAMLEGFHAMDEHFRSAPLESNLPVILGMLTVWYTNFFGAASQAVLPYSQHLK 360 AIGPEHFRAMLEGFHAMDEHFRSAPLESNLPVILGMLTVWYTNFFGAASQAVLPYSQHLK Sbjct: 301 AIGPEHFRAMLEGFHAMDEHFRSAPLESNLPVILGMLTVWYTNFFGAASQAVLPYSQHLK 360 Query: 361 RLPAYLQQLTMESNGKHITVNGEPVDYQTSPVYWGDNGINGQHSFYQMLHQGTQLVPCDF 420 RLPAYLQQLTMESNGKHITVNGEPVDYQTSPVYWGDNGINGQHSFYQMLHQGTQLVPCDF Sbjct: 361 RLPAYLQQLTMESNGKHITVNGEPVDYQTSPVYWGDNGINGQHSFYQMLHQGTQLVPCDF 420 Query: 421 IGLCQPQQALGQQHELLMANMFAQSEALAFGKDADQVRQEGTPEALVPHRVFEGNRPSNS 480 IGLCQPQQALGQQHELLMANMFAQSEALAFGKDADQVRQEGTPEALVPHRVFEGNRPSNS Sbjct: 421 IGLCQPQQALGQQHELLMANMFAQSEALAFGKDADQVRQEGTPEALVPHRVFEGNRPSNS 480 Query: 481 MLLQQLTPYSLGTLVALYEHSVFTQGVVWNIDSFDQWGVELGKQLAKRTARELIDHQQPL 540 MLLQQLTPYSLGTLVALYEHSVFTQGVVWNIDSFDQWGVELGKQLAKRTARELIDHQQPL Sbjct: 481 MLLQQLTPYSLGTLVALYEHSVFTQGVVWNIDSFDQWGVELGKQLAKRTARELIDHQQPL 540 Query: 541 HHDSSTNALIRRYRACSTATQSAARRQQAA 570 HHDSSTNALIRRYRACSTATQSAARRQQAA Sbjct: 541 HHDSSTNALIRRYRACSTATQSAARRQQAA 570 >gi|300310432|ref|YP_003774524.1| glucose-6-phosphate isomerase [Herbaspirillum seropedicae SmR1] Length = 554 Score = 568 bits (1463), Expect = e-163, Method: Compositional matrix adjust. Identities = 291/550 (52%), Positives = 366/550 (66%), Gaps = 11/550 (2%) Query: 21 SLTGKSAWRSLQEHYRDRARHFQLARLFAADDKRGESLTAEAVGIYLDYSKNLVTSETMA 80 +LT +A+++LQ H+ +A + + LFA R E + A G+ LDYSKN + SET+A Sbjct: 5 ALTDTAAFKALQHHHA-KAVTWHMRELFAQQPDRFERMHVRAAGLLLDYSKNRIESETLA 63 Query: 81 LLMQLARQCGLPDRIEAMFRGDRINLTENRSVLHTALRKPASE-RLVVDDQDVVAEVQQV 139 LLM LAR+ G+ EAMFRG++IN TE+R+VLHTALR PA VD Q V EV V Sbjct: 64 LLMSLARERGVEALREAMFRGEKINFTEHRAVLHTALRAPAQACDAQVDGQPVSQEVHAV 123 Query: 140 LRRMEGFARQVRSGQWLGHTGLPIRHVVNIGIGGSDLGPMMAYEALRPYSHDDLTLHFVS 199 L + F+ +VRSG W G+TG I +VNIGIGGS LGP M ALR Y H LT HFVS Sbjct: 124 LDHIRDFSERVRSGAWRGYTGREITDIVNIGIGGSFLGPQMVCTALRNYRHARLTTHFVS 183 Query: 200 DVDGAAMLDTVRGLPAEQTLFIVCSKTFTTLETITNATAARAWLVQQLGAEEAVARHFVG 259 +VDG + + + + E TLFIV SKTFTT ET+ NA +ARAW ++ G + +ARHFV Sbjct: 184 NVDGHDLDEVLSRVDPETTLFIVASKTFTTQETMLNAKSARAWFLRH-GQQADLARHFVA 242 Query: 260 VTTNIEEAQRFGIDADNMFGFWDWVGGRYSMTSAIGLSTMVAIGPEHFRAMLEGFHAMDE 319 V+TN E + FGID DNMF FWDWVGGRYS+ SAIGL + IG EHF A L G HAMD Sbjct: 243 VSTNAAEVESFGIDPDNMFPFWDWVGGRYSVWSAIGLPIALLIGFEHFSAFLAGAHAMDR 302 Query: 320 HFRSAPLESNLPVILGMLTVWYTNFFGAASQAVLPYSQHLKRLPAYLQQLTMESNGKHIT 379 HFR APLE+N+PVI ++ +W NFF + S +V PY Q L+ PAYLQQL MESNGK IT Sbjct: 303 HFRDAPLEANMPVIQALIGIWNRNFFNSESLSVAPYHQWLRYFPAYLQQLDMESNGKRIT 362 Query: 380 VNGEPVDYQTSPVYWGDNGINGQHSFYQMLHQGTQLVPCDFIGLCQPQQALGQQHELLMA 439 +G PV T P WGD G NGQH+F+Q+LHQGT + P DFI + +P + + ++L+A Sbjct: 363 RDGTPVGVATCPFIWGDVGTNGQHAFFQLLHQGTNVTPIDFIAVLKPSHGIDEHQDVLLA 422 Query: 440 NMFAQSEALAFGKDADQVRQE--------GTPEALVPHRVFEGNRPSNSMLLQQLTPYSL 491 N FAQSEA GK AD+VR + AL+PH+ F+GNRPSN++LL +L+P++L Sbjct: 423 NCFAQSEAFMRGKQADEVRADLERQGVDAAELAALLPHKTFDGNRPSNTLLLPELSPHAL 482 Query: 492 GTLVALYEHSVFTQGVVWNIDSFDQWGVELGKQLAKRTARELIDHQQPLHHDSSTNALIR 551 G ++ALYEH F QG +W I+SFDQWGVELGK LAK EL P HD+STNALI Sbjct: 483 GAMIALYEHKTFVQGAIWGINSFDQWGVELGKVLAKTIHGELRSEPNPALHDASTNALIA 542 Query: 552 RYRACSTATQ 561 R A Q Sbjct: 543 LARQARQADQ 552 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.134 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,720,198 Number of extensions: 70940 Number of successful extensions: 233 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 229 Number of HSP's successfully gapped: 2 Length of query: 570 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 480 Effective length of database: 1,193,720 Effective search space: 572985600 Effective search space used: 572985600 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)