BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0094.p (805 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311816|ref|YP_003775908.1| malto-oligosyltrehalose trehalo... 937 0.0 gi|300311818|ref|YP_003775910.1| 1,4-alpha-glucan branching prot... 95 3e-20 gi|300311642|ref|YP_003775734.1| 1,4-alpha-glucan branching prot... 82 2e-16 gi|300311817|ref|YP_003775909.1| glycogen debranching enzyme pro... 70 9e-13 >gi|300311816|ref|YP_003775908.1| malto-oligosyltrehalose trehalohydrolase [Herbaspirillum seropedicae SmR1] Length = 616 Score = 937 bits (2421), Expect = 0.0, Method: Compositional matrix adjust. Identities = 476/478 (99%), Positives = 478/478 (100%) Query: 328 AELAELGITAIELMPVAEFPGAHNWGYDGVLPFAPDASYGTPEQLKALIDTAHGLGMMVF 387 AELAELGITAIELMPVAEFPGAHNWGYDGVLPFAPDASYGTPEQLKALIDTAHGLGMMVF Sbjct: 139 AELAELGITAIELMPVAEFPGAHNWGYDGVLPFAPDASYGTPEQLKALIDTAHGLGMMVF 198 Query: 388 LDVVYNHFGPDGNYLHAYAPEFFRQDMQTLWGATIDFRRPEVDDFFTQNALYWLQEYRFD 447 LDVVYNHFGPDGNYLHAYAPEFFRQDMQTLWGATIDFRRPEVDDFFTQNALYWLQEYRFD Sbjct: 199 LDVVYNHFGPDGNYLHAYAPEFFRQDMQTLWGATIDFRRPEVDDFFTQNALYWLQEYRFD 258 Query: 448 GLRLDAVHAICEPAWLERLGQRIRTDIGGQRHVHLVLEHDGNAAHLLGYGHPPDDGRSSH 507 GLRLDAVHAICEPAWLERLGQRIRT+IGGQRHVHLVLEHDGNAAHLLG+GHPPDDGRSSH Sbjct: 259 GLRLDAVHAICEPAWLERLGQRIRTEIGGQRHVHLVLEHDGNAAHLLGHGHPPDDGRSSH 318 Query: 508 VYDAQWNDDAHHVLHVLLTGEDSGYYAAYAEEPAAKLARCLQQGFVYQGEVSPYSGEPRG 567 VYDAQWNDDAHHVLHVLLTGEDSGYYAAYAEEPAAKLARCLQQGFVYQGEVSPYSGEPRG Sbjct: 319 VYDAQWNDDAHHVLHVLLTGEDSGYYAAYAEEPAAKLARCLQQGFVYQGEVSPYSGEPRG 378 Query: 568 EPSADLPPTAFVNFLQNHDQIGNRAFGERLTVLAHPLALHAAQALVLLAPQIPMLFMGEE 627 EPSADLPPTAFVNFLQNHDQIGNRAFGERLTVLAHPLALHAAQALVLLAPQIPMLFMGEE Sbjct: 379 EPSADLPPTAFVNFLQNHDQIGNRAFGERLTVLAHPLALHAAQALVLLAPQIPMLFMGEE 438 Query: 628 FCAVQPFLYFTSHRDEALVKAVREGRRREFARFPQFADPALLEQIPDPNSFATFVSSIPE 687 FCAVQPFLYFTSHRDEALVKAVREGRRREFARFPQFADPALLEQIPDPNSFATFVSSIPE Sbjct: 439 FCAVQPFLYFTSHRDEALVKAVREGRRREFARFPQFADPALLEQIPDPNSFATFVSSIPE 498 Query: 688 PGPAPGSAACLRRVAELLHIRKLHIAPYLRGARALEARAIGPKAVHARWRLSNGSVLNIT 747 PGPAPGSAACLRRVAELLHIRKLHIAPYLRGARALEARAIGPKAVHARWRLSNGSVLNIT Sbjct: 499 PGPAPGSAACLRRVAELLHIRKLHIAPYLRGARALEARAIGPKAVHARWRLSNGSVLNIT 558 Query: 748 VNLAEQAVQESLDQLAQSAGADVLFDSGGALEAVAEGRFPGHAILALREPATGDGRSA 805 VNLAEQAVQESLDQLAQSAGADVLFDSGGALEAVAEGRFPGHAILALREPATGDGRSA Sbjct: 559 VNLAEQAVQESLDQLAQSAGADVLFDSGGALEAVAEGRFPGHAILALREPATGDGRSA 616 Score = 476 bits (1226), Expect = e-135, Method: Compositional matrix adjust. Identities = 240/244 (98%), Positives = 242/244 (99%) Query: 83 ASRAELGITAIELMPVAEFPGAHNWGYDGVLPFAPDASYGTPEQLKALIDTAHGLGMMVF 142 A AELGITAIELMPVAEFPGAHNWGYDGVLPFAPDASYGTPEQLKALIDTAHGLGMMVF Sbjct: 139 AELAELGITAIELMPVAEFPGAHNWGYDGVLPFAPDASYGTPEQLKALIDTAHGLGMMVF 198 Query: 143 LDVVYNHFGPDGNYLHAYAPEFFRQDMQTLWGATIDFRRPEVDDFFTQNALYWLQEYRFD 202 LDVVYNHFGPDGNYLHAYAPEFFRQDMQTLWGATIDFRRPEVDDFFTQNALYWLQEYRFD Sbjct: 199 LDVVYNHFGPDGNYLHAYAPEFFRQDMQTLWGATIDFRRPEVDDFFTQNALYWLQEYRFD 258 Query: 203 GLRLDAVHAICEPAWLERLGQRIRTDIGGQRHVHLVLEHDGNAAHLLGYGHPPDDGRSSH 262 GLRLDAVHAICEPAWLERLGQRIRT+IGGQRHVHLVLEHDGNAAHLLG+GHPPDDGRSSH Sbjct: 259 GLRLDAVHAICEPAWLERLGQRIRTEIGGQRHVHLVLEHDGNAAHLLGHGHPPDDGRSSH 318 Query: 263 VYDAQWNDDAHHVLHVLLTGEDSGYYAAYAEEPAAKLARCLQQGFVYQGEVSPYSGEPRG 322 VYDAQWNDDAHHVLHVLLTGEDSGYYAAYAEEPAAKLARCLQQGFVYQGEVSPYSGEPRG Sbjct: 319 VYDAQWNDDAHHVLHVLLTGEDSGYYAAYAEEPAAKLARCLQQGFVYQGEVSPYSGEPRG 378 Query: 323 EPSA 326 EPSA Sbjct: 379 EPSA 382 Score = 180 bits (456), Expect = 4e-46, Method: Compositional matrix adjust. Identities = 85/89 (95%), Positives = 87/89 (97%) Query: 1 MSARFSWNLPFGAQVTEPGRVRFRVWAPGARSVAVDITGRGRLPMDAEPQGWFSLETACD 60 MSARFSWNLPFGAQVTEPGRVRFRVWAPGARSVAVDITGRGRLPMDAEPQGWF+LETACD Sbjct: 1 MSARFSWNLPFGAQVTEPGRVRFRVWAPGARSVAVDITGRGRLPMDAEPQGWFALETACD 60 Query: 61 PGATYRYVFDTISQGELVAPDPASRAELG 89 PGATYRYVFDTISQGELVAPDPASR + G Sbjct: 61 PGATYRYVFDTISQGELVAPDPASRGQAG 89 >gi|300311818|ref|YP_003775910.1| 1,4-alpha-glucan branching protein [Herbaspirillum seropedicae SmR1] Length = 804 Score = 94.7 bits (234), Expect = 3e-20, Method: Compositional matrix adjust. Identities = 59/161 (36%), Positives = 81/161 (50%), Gaps = 13/161 (8%) Query: 88 LGITAIELMPVAEFPGAHNWGYDGVLPFAPDASYGTPEQLKALIDTAHGLGMMVFLDVVY 147 +G T IELMPV E P +WGY + FAP A +G PE+ A +D H G+ V LD V Sbjct: 344 MGFTHIELMPVMEHPFGGSWGYQPISMFAPSARFGKPERFAAFVDRCHQAGIGVLLDWVP 403 Query: 148 NHFGPDGN----------YLHAYAPEFFRQDMQTLWGATIDFRRPEVDDFFTQNALYWLQ 197 HF D + Y HA E F QD TL + R EV F +AL WL+ Sbjct: 404 AHFPSDQHGLAQFDGTALYEHADPREGFHQDWNTL---IFNLGRNEVRGFLIASALEWLE 460 Query: 198 EYRFDGLRLDAVHAICEPAWLERLGQRIRTDIGGQRHVHLV 238 + DGLR+DAV ++ + + G+ + GG+ ++ V Sbjct: 461 HFHVDGLRVDAVASMLYRDYSRKEGEWVPNIHGGRENLEAV 501 Score = 94.7 bits (234), Expect = 3e-20, Method: Compositional matrix adjust. Identities = 59/161 (36%), Positives = 81/161 (50%), Gaps = 13/161 (8%) Query: 333 LGITAIELMPVAEFPGAHNWGYDGVLPFAPDASYGTPEQLKALIDTAHGLGMMVFLDVVY 392 +G T IELMPV E P +WGY + FAP A +G PE+ A +D H G+ V LD V Sbjct: 344 MGFTHIELMPVMEHPFGGSWGYQPISMFAPSARFGKPERFAAFVDRCHQAGIGVLLDWVP 403 Query: 393 NHFGPDGN----------YLHAYAPEFFRQDMQTLWGATIDFRRPEVDDFFTQNALYWLQ 442 HF D + Y HA E F QD TL + R EV F +AL WL+ Sbjct: 404 AHFPSDQHGLAQFDGTALYEHADPREGFHQDWNTL---IFNLGRNEVRGFLIASALEWLE 460 Query: 443 EYRFDGLRLDAVHAICEPAWLERLGQRIRTDIGGQRHVHLV 483 + DGLR+DAV ++ + + G+ + GG+ ++ V Sbjct: 461 HFHVDGLRVDAVASMLYRDYSRKEGEWVPNIHGGRENLEAV 501 >gi|300311642|ref|YP_003775734.1| 1,4-alpha-glucan branching protein [Herbaspirillum seropedicae SmR1] Length = 819 Score = 81.6 bits (200), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 53/161 (32%), Positives = 75/161 (46%), Gaps = 13/161 (8%) Query: 88 LGITAIELMPVAEFPGAHNWGYDGVLPFAPDASYGTPEQLKALIDTAHGLGMMVFLDVVY 147 +G T +EL+PV E P +WGY + FAP A +G P + +D H G+ V LD V Sbjct: 370 MGFTHVELLPVMEHPFGGSWGYQPLSQFAPSARFGPPAEFATFVDACHRAGIGVLLDWVP 429 Query: 148 NHFGPDGN----------YLHAYAPEFFRQDMQTLWGATIDFRRPEVDDFFTQNALYWLQ 197 HF D + Y H E QD T + R EV F +AL WL+ Sbjct: 430 AHFPSDPHGLAYFDGTPLYEHGDPREGIHQDWNT---HIYNLGRHEVRGFLIASALEWLE 486 Query: 198 EYRFDGLRLDAVHAICEPAWLERLGQRIRTDIGGQRHVHLV 238 + DGLR+DAV ++ + GQ + GG+ ++ V Sbjct: 487 HFHVDGLRVDAVASMLYRDYSRSSGQWVPNIHGGRENLEAV 527 Score = 81.6 bits (200), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 53/161 (32%), Positives = 75/161 (46%), Gaps = 13/161 (8%) Query: 333 LGITAIELMPVAEFPGAHNWGYDGVLPFAPDASYGTPEQLKALIDTAHGLGMMVFLDVVY 392 +G T +EL+PV E P +WGY + FAP A +G P + +D H G+ V LD V Sbjct: 370 MGFTHVELLPVMEHPFGGSWGYQPLSQFAPSARFGPPAEFATFVDACHRAGIGVLLDWVP 429 Query: 393 NHFGPDGN----------YLHAYAPEFFRQDMQTLWGATIDFRRPEVDDFFTQNALYWLQ 442 HF D + Y H E QD T + R EV F +AL WL+ Sbjct: 430 AHFPSDPHGLAYFDGTPLYEHGDPREGIHQDWNT---HIYNLGRHEVRGFLIASALEWLE 486 Query: 443 EYRFDGLRLDAVHAICEPAWLERLGQRIRTDIGGQRHVHLV 483 + DGLR+DAV ++ + GQ + GG+ ++ V Sbjct: 487 HFHVDGLRVDAVASMLYRDYSRSSGQWVPNIHGGRENLEAV 527 >gi|300311817|ref|YP_003775909.1| glycogen debranching enzyme protein [Herbaspirillum seropedicae SmR1] Length = 753 Score = 69.7 bits (169), Expect = 9e-13, Method: Compositional matrix adjust. Identities = 50/156 (32%), Positives = 67/156 (42%), Gaps = 26/156 (16%) Query: 323 EPSAHAELAELGITAIELMPVAEF--------PGAHN-WGYDGVLPFAPDASY---GTPE 370 +P+ L LG+T +ELMPV F G HN WGY + FAP+ Y G P Sbjct: 195 DPAFIDHLLRLGVTTLELMPVHAFLQDSFLVERGLHNYWGYSTLSYFAPEPGYLSMGDPN 254 Query: 371 QLKALIDTAHGLGMMVFLDVVYNH------FGPDGNYLHAYAPEFFR--------QDMQT 416 +L+ + H G+ V LDVVYNH GP ++ +FR T Sbjct: 255 ELRMAVRRLHSAGIEVILDVVYNHTSCGSELGPTLSFRGLDNASYFRLLPGQERYHINDT 314 Query: 417 LWGATIDFRRPEVDDFFTQNALYWLQEYRFDGLRLD 452 G T++ P V + YW Q + DG R D Sbjct: 315 GCGNTLNLSHPRVLQMVLDSLRYWAQSFHIDGFRFD 350 Score = 68.2 bits (165), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 48/146 (32%), Positives = 63/146 (43%), Gaps = 26/146 (17%) Query: 88 LGITAIELMPVAEF--------PGAHN-WGYDGVLPFAPDASY---GTPEQLKALIDTAH 135 LG+T +ELMPV F G HN WGY + FAP+ Y G P +L+ + H Sbjct: 205 LGVTTLELMPVHAFLQDSFLVERGLHNYWGYSTLSYFAPEPGYLSMGDPNELRMAVRRLH 264 Query: 136 GLGMMVFLDVVYNH------FGPDGNYLHAYAPEFFR--------QDMQTLWGATIDFRR 181 G+ V LDVVYNH GP ++ +FR T G T++ Sbjct: 265 SAGIEVILDVVYNHTSCGSELGPTLSFRGLDNASYFRLLPGQERYHINDTGCGNTLNLSH 324 Query: 182 PEVDDFFTQNALYWLQEYRFDGLRLD 207 P V + YW Q + DG R D Sbjct: 325 PRVLQMVLDSLRYWAQSFHIDGFRFD 350 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.139 0.438 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,911,166 Number of extensions: 135781 Number of successful extensions: 392 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 383 Number of HSP's successfully gapped: 9 Length of query: 805 Length of database: 1,619,870 Length adjustment: 93 Effective length of query: 712 Effective length of database: 1,179,515 Effective search space: 839814680 Effective search space used: 839814680 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 134 (56.2 bits)