BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0093.p (753 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311817|ref|YP_003775909.1| glycogen debranching enzyme pro... 1550 0.0 gi|300311816|ref|YP_003775908.1| malto-oligosyltrehalose trehalo... 88 3e-18 gi|300311818|ref|YP_003775910.1| 1,4-alpha-glucan branching prot... 64 3e-11 >gi|300311817|ref|YP_003775909.1| glycogen debranching enzyme protein [Herbaspirillum seropedicae SmR1] Length = 753 Score = 1550 bits (4014), Expect = 0.0, Method: Compositional matrix adjust. Identities = 753/753 (100%), Positives = 753/753 (100%) Query: 1 MSVAFPDRLMPGLPYPLGASSDGLGVNFAVFSAHATRMDLCLFDPSGKRELKRIALPECT 60 MSVAFPDRLMPGLPYPLGASSDGLGVNFAVFSAHATRMDLCLFDPSGKRELKRIALPECT Sbjct: 1 MSVAFPDRLMPGLPYPLGASSDGLGVNFAVFSAHATRMDLCLFDPSGKRELKRIALPECT 60 Query: 61 DEVWHGYLPGAAPGLVYGFRAHGPYEPNKGHRFNPNKLLLDPYAKRLVGQVRWTDALYGY 120 DEVWHGYLPGAAPGLVYGFRAHGPYEPNKGHRFNPNKLLLDPYAKRLVGQVRWTDALYGY Sbjct: 61 DEVWHGYLPGAAPGLVYGFRAHGPYEPNKGHRFNPNKLLLDPYAKRLVGQVRWTDALYGY 120 Query: 121 RLHSPRADLSFDRRDSAAAMPKAMVTEDAFVWEHDRLPQVPWNKTVIYEAHVKGISRLRE 180 RLHSPRADLSFDRRDSAAAMPKAMVTEDAFVWEHDRLPQVPWNKTVIYEAHVKGISRLRE Sbjct: 121 RLHSPRADLSFDRRDSAAAMPKAMVTEDAFVWEHDRLPQVPWNKTVIYEAHVKGISRLRE 180 Query: 181 DLLPHERGTFAALADPAFIDHLLRLGVTTLELMPVHAFLQDSFLVERGLHNYWGYSTLSY 240 DLLPHERGTFAALADPAFIDHLLRLGVTTLELMPVHAFLQDSFLVERGLHNYWGYSTLSY Sbjct: 181 DLLPHERGTFAALADPAFIDHLLRLGVTTLELMPVHAFLQDSFLVERGLHNYWGYSTLSY 240 Query: 241 FAPEPGYLSMGDPNELRMAVRRLHSAGIEVILDVVYNHTSCGSELGPTLSFRGLDNASYF 300 FAPEPGYLSMGDPNELRMAVRRLHSAGIEVILDVVYNHTSCGSELGPTLSFRGLDNASYF Sbjct: 241 FAPEPGYLSMGDPNELRMAVRRLHSAGIEVILDVVYNHTSCGSELGPTLSFRGLDNASYF 300 Query: 301 RLLPGQERYHINDTGCGNTLNLSHPRVLQMVLDSLRYWAQSFHIDGFRFDLGSTLGREPH 360 RLLPGQERYHINDTGCGNTLNLSHPRVLQMVLDSLRYWAQSFHIDGFRFDLGSTLGREPH Sbjct: 301 RLLPGQERYHINDTGCGNTLNLSHPRVLQMVLDSLRYWAQSFHIDGFRFDLGSTLGREPH 360 Query: 361 GFDPGSGFFDALLQDPVLSRLKLISEPWDIGPGGYQLGNHPARFGEWNDKFRDDMRKFWK 420 GFDPGSGFFDALLQDPVLSRLKLISEPWDIGPGGYQLGNHPARFGEWNDKFRDDMRKFWK Sbjct: 361 GFDPGSGFFDALLQDPVLSRLKLISEPWDIGPGGYQLGNHPARFGEWNDKFRDDMRKFWK 420 Query: 421 GDEAMRGAFAQRMMGSAELFEHHRRKPWASINFIASHDGFTLRDLVSYNGKHNEANGENN 480 GDEAMRGAFAQRMMGSAELFEHHRRKPWASINFIASHDGFTLRDLVSYNGKHNEANGENN Sbjct: 421 GDEAMRGAFAQRMMGSAELFEHHRRKPWASINFIASHDGFTLRDLVSYNGKHNEANGENN 480 Query: 481 NDGHNDNASYNWGAEGDTDDAAILAKRACVQRAMLASTLLAQGTPMLLAGDEFHRSQGGN 540 NDGHNDNASYNWGAEGDTDDAAILAKRACVQRAMLASTLLAQGTPMLLAGDEFHRSQGGN Sbjct: 481 NDGHNDNASYNWGAEGDTDDAAILAKRACVQRAMLASTLLAQGTPMLLAGDEFHRSQGGN 540 Query: 541 NNAYCQDNEISWVNWDQARSPEGAALSAFVGRVTAIRRDFEVLRASYFLHGSQMVADGLP 600 NNAYCQDNEISWVNWDQARSPEGAALSAFVGRVTAIRRDFEVLRASYFLHGSQMVADGLP Sbjct: 541 NNAYCQDNEISWVNWDQARSPEGAALSAFVGRVTAIRRDFEVLRASYFLHGSQMVADGLP 600 Query: 601 DVAWFDEAARPVSSEDWNNPVARAITLQLAGKPQESHKQLSSRLHDAGQSDSPAEAAEAT 660 DVAWFDEAARPVSSEDWNNPVARAITLQLAGKPQESHKQLSSRLHDAGQSDSPAEAAEAT Sbjct: 601 DVAWFDEAARPVSSEDWNNPVARAITLQLAGKPQESHKQLSSRLHDAGQSDSPAEAAEAT 660 Query: 661 PDVLMIFVNAGHEAIDFHFPRTEGDYHLLLDTAQDTGIPTGEDVRLVLPGSATVQVPAHS 720 PDVLMIFVNAGHEAIDFHFPRTEGDYHLLLDTAQDTGIPTGEDVRLVLPGSATVQVPAHS Sbjct: 661 PDVLMIFVNAGHEAIDFHFPRTEGDYHLLLDTAQDTGIPTGEDVRLVLPGSATVQVPAHS 720 Query: 721 LQVFANRPLHLASAIYPLAANEWIARNEWGERP 753 LQVFANRPLHLASAIYPLAANEWIARNEWGERP Sbjct: 721 LQVFANRPLHLASAIYPLAANEWIARNEWGERP 753 >gi|300311816|ref|YP_003775908.1| malto-oligosyltrehalose trehalohydrolase [Herbaspirillum seropedicae SmR1] Length = 616 Score = 87.8 bits (216), Expect = 3e-18, Method: Compositional matrix adjust. Identities = 65/210 (30%), Positives = 89/210 (42%), Gaps = 40/210 (19%) Query: 141 PKAMVTEDAFVWEHDRLPQVPWNKTVIYEAHVKGISRLREDLLPHERGTFAALADPAFID 200 P +V A+VW++ + PW +TV+YE H P G FA + + Sbjct: 94 PSLVVDAQAYVWQYPQWRGRPWEETVLYELH------------PGAMGGFAGIRQR--LA 139 Query: 201 HLLRLGVTTLELMPVHAFLQDSFLVERGLHNYWGYSTLSYFAPEPGYLSMGDPNELRMAV 260 L LG+T +ELMPV F G HN WGY + FAP+ Y G P +L+ + Sbjct: 140 ELAELGITAIELMPVAEF--------PGAHN-WGYDGVLPFAPDASY---GTPEQLKALI 187 Query: 261 RRLHSAGIEVILDVVYNHTSCGSELGPTLSFRGLDNASYFRLLPGQERYHINDTGCGNTL 320 H G+ V LDVVYNH GP ++ +FR T G T+ Sbjct: 188 DTAHGLGMMVFLDVVYNH------FGPDGNYLHAYAPEFFR--------QDMQTLWGATI 233 Query: 321 NLSHPRVLQMVLDSLRYWAQSFHIDGFRFD 350 + P V + YW Q + DG R D Sbjct: 234 DFRRPEVDDFFTQNALYWLQEYRFDGLRLD 263 >gi|300311818|ref|YP_003775910.1| 1,4-alpha-glucan branching protein [Herbaspirillum seropedicae SmR1] Length = 804 Score = 64.3 bits (155), Expect = 3e-11, Method: Compositional matrix adjust. Identities = 89/340 (26%), Positives = 131/340 (38%), Gaps = 62/340 (18%) Query: 22 DGL-GVNFAVFSAHATRMDLC--LFDPSGKRELKRIALPECTDEVWHGYLPGAAPGLVYG 78 DG+ G FAV++ +A R+ + G+R + R+ VW ++P PG VY Sbjct: 192 DGVPGTRFAVWAPNAERVSVVGDFNQWDGRRHVMRL---RHEPGVWELFIPRIGPGDVYK 248 Query: 79 FRAHGPYEPNKGHRFNPNKLLLDPYAKRLVGQVRWTDALYGYRLHSPRADLSFDRRDSAA 138 + G +G P K DP A RA S Sbjct: 249 YEIRG-----RGGVLLPLKA--DPVA---------------------RATEPPPATASVV 280 Query: 139 AMPKAMVTEDAFVWEHDRLPQVPWNKTV-IYEAHVKGISRLREDLLPHERGTFAALADPA 197 A + V +D W +R + + + IYE H R+ E H + LAD Sbjct: 281 ADARPYVWQDQ-QWMAERAQRQSLSAPMSIYEVHPASWRRVPEQ--DHRNLNWDELAD-Q 336 Query: 198 FIDHLLRLGVTTLELMPVHAFLQDSFLVERGLHNYWGYSTLSYFAPEPGYLSMGDPNELR 257 I +++ +G T +ELMPV +E WGY +S FAP + G P Sbjct: 337 LIPYVVGMGFTHIELMPV---------MEHPFGGSWGYQPISMFAPSARF---GKPERFA 384 Query: 258 MAVRRLHSAGIEVILDVVYNHTSCGSELGPTLSFRGLDNASYFRLLPGQERYHINDTGCG 317 V R H AGI V+LD V H D + + +E +H + Sbjct: 385 AFVDRCHQAGIGVLLDWVPAHFPSDQH-----GLAQFDGTALYEHADPREGFHQD----W 435 Query: 318 NTL--NLSHPRVLQMVLDSLRYWAQSFHIDGFRFDLGSTL 355 NTL NL V ++ S W + FH+DG R D +++ Sbjct: 436 NTLIFNLGRNEVRGFLIASALEWLEHFHVDGLRVDAVASM 475 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.137 0.433 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,635,024 Number of extensions: 120073 Number of successful extensions: 275 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 271 Number of HSP's successfully gapped: 4 Length of query: 753 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 661 Effective length of database: 1,184,250 Effective search space: 782789250 Effective search space used: 782789250 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 134 (56.2 bits)