BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0092.p (761 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311818|ref|YP_003775910.1| 1,4-alpha-glucan branching prot... 1565 0.0 gi|300311642|ref|YP_003775734.1| 1,4-alpha-glucan branching prot... 994 0.0 gi|300311816|ref|YP_003775908.1| malto-oligosyltrehalose trehalo... 122 7e-29 gi|300311817|ref|YP_003775909.1| glycogen debranching enzyme pro... 64 5e-11 >gi|300311818|ref|YP_003775910.1| 1,4-alpha-glucan branching protein [Herbaspirillum seropedicae SmR1] Length = 804 Score = 1565 bits (4052), Expect = 0.0, Method: Compositional matrix adjust. Identities = 761/761 (100%), Positives = 761/761 (100%) Query: 1 LSLSFEEQGMLEALLAGRLTQPFDLLGPRYASAKGDGAAVRCFHPGAEAVRVRCRHSGVL 60 LSLSFEEQGMLEALLAGRLTQPFDLLGPRYASAKGDGAAVRCFHPGAEAVRVRCRHSGVL Sbjct: 44 LSLSFEEQGMLEALLAGRLTQPFDLLGPRYASAKGDGAAVRCFHPGAEAVRVRCRHSGVL 103 Query: 61 LAELQQYPVDGQPSGFFSGLVPGLEQRRHEVPYVLEVDWRIPDGSRSVQSTEDPYAFGLL 120 LAELQQYPVDGQPSGFFSGLVPGLEQRRHEVPYVLEVDWRIPDGSRSVQSTEDPYAFGLL Sbjct: 104 LAELQQYPVDGQPSGFFSGLVPGLEQRRHEVPYVLEVDWRIPDGSRSVQSTEDPYAFGLL 163 Query: 121 LGELDLHLLQEGRHRQLADCLGARPMEIDGVPGTRFAVWAPNAERVSVVGDFNQWDGRRH 180 LGELDLHLLQEGRHRQLADCLGARPMEIDGVPGTRFAVWAPNAERVSVVGDFNQWDGRRH Sbjct: 164 LGELDLHLLQEGRHRQLADCLGARPMEIDGVPGTRFAVWAPNAERVSVVGDFNQWDGRRH 223 Query: 181 VMRLRHEPGVWELFIPRIGPGDVYKYEIRGRGGVLLPLKADPVARATEPPPATASVVADA 240 VMRLRHEPGVWELFIPRIGPGDVYKYEIRGRGGVLLPLKADPVARATEPPPATASVVADA Sbjct: 224 VMRLRHEPGVWELFIPRIGPGDVYKYEIRGRGGVLLPLKADPVARATEPPPATASVVADA 283 Query: 241 RPYVWQDQQWMAERAQRQSLSAPMSIYEVHPASWRRVPEQDHRNLNWDELADQLIPYVVG 300 RPYVWQDQQWMAERAQRQSLSAPMSIYEVHPASWRRVPEQDHRNLNWDELADQLIPYVVG Sbjct: 284 RPYVWQDQQWMAERAQRQSLSAPMSIYEVHPASWRRVPEQDHRNLNWDELADQLIPYVVG 343 Query: 301 MGFTHIELMPVMEHPFGGSWGYQPISMFAPSARFGKPERFAAFVDRCHQAGIGVLLDWVP 360 MGFTHIELMPVMEHPFGGSWGYQPISMFAPSARFGKPERFAAFVDRCHQAGIGVLLDWVP Sbjct: 344 MGFTHIELMPVMEHPFGGSWGYQPISMFAPSARFGKPERFAAFVDRCHQAGIGVLLDWVP 403 Query: 361 AHFPSDQHGLAQFDGTALYEHADPREGFHQDWNTLIFNLGRNEVRGFLIASALEWLEHFH 420 AHFPSDQHGLAQFDGTALYEHADPREGFHQDWNTLIFNLGRNEVRGFLIASALEWLEHFH Sbjct: 404 AHFPSDQHGLAQFDGTALYEHADPREGFHQDWNTLIFNLGRNEVRGFLIASALEWLEHFH 463 Query: 421 VDGLRVDAVASMLYRDYSRKEGEWVPNIHGGRENLEAVAFLRELNTVVAQRCPGALMIAE 480 VDGLRVDAVASMLYRDYSRKEGEWVPNIHGGRENLEAVAFLRELNTVVAQRCPGALMIAE Sbjct: 464 VDGLRVDAVASMLYRDYSRKEGEWVPNIHGGRENLEAVAFLRELNTVVAQRCPGALMIAE 523 Query: 481 ESTSWPGVTAPVKEEGDHHGLGFSYKWNMGWMNDTLRYMQHDPLFRRYHHHDMTFGLLYG 540 ESTSWPGVTAPVKEEGDHHGLGFSYKWNMGWMNDTLRYMQHDPLFRRYHHHDMTFGLLYG Sbjct: 524 ESTSWPGVTAPVKEEGDHHGLGFSYKWNMGWMNDTLRYMQHDPLFRRYHHHDMTFGLLYG 583 Query: 541 FSENFILPISHDEVVHGKRALLNKMPGDQWRRFANVRAYFGFMWTHPGKKLLFMGSEFGQ 600 FSENFILPISHDEVVHGKRALLNKMPGDQWRRFANVRAYFGFMWTHPGKKLLFMGSEFGQ Sbjct: 584 FSENFILPISHDEVVHGKRALLNKMPGDQWRRFANVRAYFGFMWTHPGKKLLFMGSEFGQ 643 Query: 601 PDEFDHDKSPYWHLLDEHAGHPAEHRQLRQLVGDLNRLYCSEPALHQRDCRADGFAWAVG 660 PDEFDHDKSPYWHLLDEHAGHPAEHRQLRQLVGDLNRLYCSEPALHQRDCRADGFAWAVG Sbjct: 644 PDEFDHDKSPYWHLLDEHAGHPAEHRQLRQLVGDLNRLYCSEPALHQRDCRADGFAWAVG 703 Query: 661 DDSVNSVLAYFRYADEGPPVLVVVNLTPVPRVNYRIGVPSIAPGHHGVWREVLNTDAGLY 720 DDSVNSVLAYFRYADEGPPVLVVVNLTPVPRVNYRIGVPSIAPGHHGVWREVLNTDAGLY Sbjct: 704 DDSVNSVLAYFRYADEGPPVLVVVNLTPVPRVNYRIGVPSIAPGHHGVWREVLNTDAGLY 763 Query: 721 GGSNNGNLGAVQVQELAWHGHAASIELTLPPLSTLILRRES 761 GGSNNGNLGAVQVQELAWHGHAASIELTLPPLSTLILRRES Sbjct: 764 GGSNNGNLGAVQVQELAWHGHAASIELTLPPLSTLILRRES 804 >gi|300311642|ref|YP_003775734.1| 1,4-alpha-glucan branching protein [Herbaspirillum seropedicae SmR1] Length = 819 Score = 994 bits (2570), Expect = 0.0, Method: Compositional matrix adjust. Identities = 512/794 (64%), Positives = 594/794 (74%), Gaps = 54/794 (6%) Query: 7 EQGMLEALLAGRLTQPFDLLGPRY------ASAKGDGAAVR--CFHPGAEAVRVRCRHS- 57 ++ ++ L+ GRL PF LLGPR A+ K +R C+ PGA AV+V R S Sbjct: 38 DESLVARLMQGRLGDPFALLGPREIADAPEATQKAKAKRLRLCCYFPGASAVQVYARTSA 97 Query: 58 -----------GVLLAELQQY-PVDGQP------------SGFFSGLVPGLEQRRHE--V 91 G LL L ++ P GQ SG F G V L +R + + Sbjct: 98 APDELHAPDSTGSLLGSLHEWSPATGQGESDHDQEAPRQRSGVFFGEVK-LPRRAVQPPI 156 Query: 92 PYVLEVDWRIPDGSRSVQSTEDPYAFGLLLGELDLHLLQEGRHRQLADCLGARPMEIDGV 151 PYV V WR DG ++Q TED YAFGLLL + DLHL +EGRHRQLA CLG++ M+IDGV Sbjct: 157 PYVFHVTWRGADGRETLQITEDAYAFGLLLSDFDLHLFREGRHRQLARCLGSQVMQIDGV 216 Query: 152 PGTRFAVWAPNAERVSVVGDFNQWDGRRHVMRLRHEPGVWELFIPRIGPGDVYKYEIRGR 211 G RFAVWAPNA+RVSV+GDFNQWDGRR+ MRLR GVWELF+PR+G G +YKYEI G Sbjct: 217 EGVRFAVWAPNAQRVSVIGDFNQWDGRRNPMRLRQSAGVWELFVPRLGDGALYKYEIIGP 276 Query: 212 GGVLLPLKADPVARATEPPPATASVVADARPYV----WQDQQWMAERAQRQSLSAPMSIY 267 G LLP KADP+ARATE PPATAS V D V WQD W+ +RAQ Q+ APMSIY Sbjct: 277 HGELLPGKADPMARATEVPPATASRVIDDSKLVSGRRWQDAHWLQQRAQGQTRQAPMSIY 336 Query: 268 EVHPASWRRVPEQDHRNLNWDELADQLIPYVVGMGFTHIELMPVMEHPFGGSWGYQPISM 327 EVH ASW R+P + +RNL+W+ELAD+LIPYV GMGFTH+EL+PVMEHPFGGSWGYQP+S Sbjct: 337 EVHAASWLRLPGEGNRNLDWNELADRLIPYVKGMGFTHVELLPVMEHPFGGSWGYQPLSQ 396 Query: 328 FAPSARFGKPERFAAFVDRCHQAGIGVLLDWVPAHFPSDQHGLAQFDGTALYEHADPREG 387 FAPSARFG P FA FVD CH+AGIGVLLDWVPAHFPSD HGLA FDGT LYEH DPREG Sbjct: 397 FAPSARFGPPAEFATFVDACHRAGIGVLLDWVPAHFPSDPHGLAYFDGTPLYEHGDPREG 456 Query: 388 FHQDWNTLIFNLGRNEVRGFLIASALEWLEHFHVDGLRVDAVASMLYRDYSRKEGEWVPN 447 HQDWNT I+NLGR+EVRGFLIASALEWLEHFHVDGLRVDAVASMLYRDYSR G+WVPN Sbjct: 457 IHQDWNTHIYNLGRHEVRGFLIASALEWLEHFHVDGLRVDAVASMLYRDYSRSSGQWVPN 516 Query: 448 IHGGRENLEAVAFLRELNTVVAQRCPGALMIAEESTSWPGVTAPVKEEGDHHGLGFSYKW 507 IHGGRENLEAV FLRELN VA+RCPGALMIAEEST+WPGVTAPV E GLGF+YKW Sbjct: 517 IHGGRENLEAVDFLRELNQTVAERCPGALMIAEESTAWPGVTAPVSEG----GLGFTYKW 572 Query: 508 NMGWMNDTLRYMQHDPLFRRYHHHDMTFGLLYGFSENFILPISHDEVVHGKRALLNKMPG 567 NMGWM+DTLRY++ D ++RRYHHHD+TFG++Y FSE+F+LPISHDEVVHGK LL KM G Sbjct: 573 NMGWMHDTLRYVELDSIYRRYHHHDITFGMIYAFSESFVLPISHDEVVHGKGTLLQKMAG 632 Query: 568 DQWRRFANVRAYFGFMWTHPGKKLLFMGSEFGQPDEFDHDKSPYWHLLDEHAGHPAEHRQ 627 D W+RFAN+RAY GFMWTHPGKKLLFMG EF + EFD D+SP+W LLD+ A HR Sbjct: 633 DAWQRFANLRAYLGFMWTHPGKKLLFMGCEFAETREFDPDRSPHWDLLDD-----AHHRG 687 Query: 628 LRQLVGDLNRLYCSEPALHQRDCRADGFAWAVGDDSVNSVLAYFRYADEGPPVLVVVNLT 687 +++LV DLNRLY PAL++ D A GF+W VG+D +NSV AY R D+ PVLVV+N+T Sbjct: 688 VQRLVRDLNRLYAGTPALYRHDTEAKGFSWVVGNDELNSVFAYLRQTDDEAPVLVVINMT 747 Query: 688 PVPRVNYRIGVPSIAPG--HHGVWREVLNTDAGLYGGSNNGNLGAVQVQELAWHGHAASI 745 PVPR +YRIGVP PG W E+LNTDA YGGS+ GN G ++ Q A HGHA+S+ Sbjct: 748 PVPRHHYRIGVP---PGQTQQDRWEEILNTDADCYGGSHLGNGGQLRAQAQACHGHASSL 804 Query: 746 ELTLPPLSTLILRR 759 LTLPPLSTL+LRR Sbjct: 805 VLTLPPLSTLVLRR 818 >gi|300311816|ref|YP_003775908.1| malto-oligosyltrehalose trehalohydrolase [Herbaspirillum seropedicae SmR1] Length = 616 Score = 122 bits (307), Expect = 7e-29, Method: Compositional matrix adjust. Identities = 101/310 (32%), Positives = 138/310 (44%), Gaps = 41/310 (13%) Query: 154 TRFAVWAPNAERVSVVGDFNQWDGRRHVMRLRHEPGVWELFIPRIGPGDVYKY--EIRGR 211 RF VWAP A V+V D R + + EP W PG Y+Y + + Sbjct: 21 VRFRVWAPGARSVAV--DITG----RGRLPMDAEPQGWFALETACDPGATYRYVFDTISQ 74 Query: 212 GGVLLPLKADPVARATEPPPATASVVADARPYVWQDQQWMAERAQRQSLSAPMSIYEVHP 271 G ++ P DP +R S+V DA+ YVWQ QW + L YE+HP Sbjct: 75 GELVAP---DPASRGQAGDVFDPSLVVDAQAYVWQYPQWRGRPWEETVL------YELHP 125 Query: 272 ASWRRVPEQDHRNLNWDELADQLIPYVVGMGFTHIELMPVMEHPFGGSWGYQPISMFAPS 331 + R ELA+ +G T IELMPV E P +WGY + FAP Sbjct: 126 GAMGGFAGIRQR---LAELAE--------LGITAIELMPVAEFPGAHNWGYDGVLPFAPD 174 Query: 332 ARFGKPERFAAFVDRCHQAGIGVLLDWVPAHFPSDQHGLAQFDGTALYEHADPREGFHQD 391 A +G PE+ A +D H G+ V LD V HF D + Y HA E F QD Sbjct: 175 ASYGTPEQLKALIDTAHGLGMMVFLDVVYNHFGPDGN----------YLHAYAPEFFRQD 224 Query: 392 WNTL---IFNLGRNEVRGFLIASALEWLEHFHVDGLRVDAVASMLYRDYSRKEGEWVPNI 448 TL + R EV F +AL WL+ + DGLR+DAV ++ + + G+ + Sbjct: 225 MQTLWGATIDFRRPEVDDFFTQNALYWLQEYRFDGLRLDAVHAICEPAWLERLGQRIRTE 284 Query: 449 HGGRENLEAV 458 GG+ ++ V Sbjct: 285 IGGQRHVHLV 294 >gi|300311817|ref|YP_003775909.1| glycogen debranching enzyme protein [Herbaspirillum seropedicae SmR1] Length = 753 Score = 63.9 bits (154), Expect = 5e-11, Method: Compositional matrix adjust. Identities = 89/340 (26%), Positives = 131/340 (38%), Gaps = 62/340 (18%) Query: 149 DGVPGTRFAVWAPNAERVSVVGDFNQWDGRRHVMRL---RHEPGVWELFIPRIGPGDVYK 205 DG+ G FAV++ +A R+ + G+R + R+ VW ++P PG VY Sbjct: 22 DGL-GVNFAVFSAHATRMDLC--LFDPSGKRELKRIALPECTDEVWHGYLPGAAPGLVYG 78 Query: 206 YEIRG-----RGGVLLPLKA--DPVA---------------------RATEPPPATASVV 237 + G +G P K DP A RA S Sbjct: 79 FRAHGPYEPNKGHRFNPNKLLLDPYAKRLVGQVRWTDALYGYRLHSPRADLSFDRRDSAA 138 Query: 238 ADARPYVWQDQ-QWMAERAQRQSLSAPMSIYEVHPASWRRVPEQ--DHRNLNWDELAD-Q 293 A + V +D W +R + + + IYE H R+ E H + LAD Sbjct: 139 AMPKAMVTEDAFVWEHDRLPQVPWNKTV-IYEAHVKGISRLREDLLPHERGTFAALADPA 197 Query: 294 LIPYVVGMGFTHIELMPV---------MEHPFGGSWGYQPISMFAPSARF---GKPERFA 341 I +++ +G T +ELMPV +E WGY +S FAP + G P Sbjct: 198 FIDHLLRLGVTTLELMPVHAFLQDSFLVERGLHNYWGYSTLSYFAPEPGYLSMGDPNELR 257 Query: 342 AFVDRCHQAGIGVLLDWVPAHFPSDQH-----GLAQFDGTALYEHADPREGFHQD----W 392 V R H AGI V+LD V H D + + +E +H + Sbjct: 258 MAVRRLHSAGIEVILDVVYNHTSCGSELGPTLSFRGLDNASYFRLLPGQERYHINDTGCG 317 Query: 393 NTLIFNLGRNEVRGFLIASALEWLEHFHVDGLRVDAVASM 432 NTL NL V ++ S W + FH+DG R D +++ Sbjct: 318 NTL--NLSHPRVLQMVLDSLRYWAQSFHIDGFRFDLGSTL 355 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.140 0.455 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,719,593 Number of extensions: 124475 Number of successful extensions: 310 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 301 Number of HSP's successfully gapped: 4 Length of query: 761 Length of database: 1,619,870 Length adjustment: 93 Effective length of query: 668 Effective length of database: 1,179,515 Effective search space: 787916020 Effective search space used: 787916020 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 134 (56.2 bits)