BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0084.p (330 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311826|ref|YP_003775918.1| D-lactate dehydrogenase [Herbas... 660 0.0 gi|300310960|ref|YP_003775052.1| dehydrogenase oxidoreductase [H... 131 7e-32 gi|300313735|ref|YP_003777827.1| D-3-phosphoglycerate dehydrogen... 130 2e-31 gi|300311988|ref|YP_003776080.1| D-3-phosphoglycerate dehydrogen... 120 2e-28 gi|300310832|ref|YP_003774924.1| D-isomer specific 2-hydroxyacid... 109 3e-25 >gi|300311826|ref|YP_003775918.1| D-lactate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 330 Score = 660 bits (1702), Expect = 0.0, Method: Compositional matrix adjust. Identities = 329/330 (99%), Positives = 330/330 (100%) Query: 1 MKTAVFSARRYDQTLLARANAAHGHELVFLQDRLEAGTAQLAAGCQAVAAFVNDDLGGVV 60 MKTAVFSARRYDQTLLARANAAHGHELVFLQDRLEAGTAQLAAGCQAVAAFVNDDLGGVV Sbjct: 1 MKTAVFSARRYDQTLLARANAAHGHELVFLQDRLEAGTAQLAAGCQAVAAFVNDDLGGVV 60 Query: 61 LEKLAELGVRFVTTRSTGFNHIDVAAARALGLTVARVADYSPYSVAEFAVGLLQAVNRKI 120 LEKLAELGVRFVTTRSTGFNHIDVAAARALGLTVARVADYSPYSVAEFAVGLLQAVNRKI Sbjct: 61 LEKLAELGVRFVTTRSTGFNHIDVAAARALGLTVARVADYSPYSVAEFAVGLLQAVNRKI 120 Query: 121 ARASMRTREGNFELDGLMGVDLHGKTVGVIGTGKIGVLFARIMAGFGCQILGSDVHRNPD 180 ARASMRTREGNFELDGLMGVDLHGKTVGVIGTGKIGVLFARIMAGFGCQILGSDVHRNPD Sbjct: 121 ARASMRTREGNFELDGLMGVDLHGKTVGVIGTGKIGVLFARIMAGFGCQILGSDVHRNPD 180 Query: 181 FDALGGRYVERTELFAASDVISLHCPLTEATRYLVNAQSLALAKPGCILVNTSRGGLVDT 240 FDALGGRYVERTELFAASDVISLHCPLTEATRYLVNAQSLALAKPGCILVNTSRGGLVDT Sbjct: 181 FDALGGRYVERTELFAASDVISLHCPLTEATRYLVNAQSLALAKPGCILVNTSRGGLVDT 240 Query: 241 EAAIAALKSGRLRGLAIDVYEQEASLFFQDLSSTIITDDVIQRLVSFPNVIVTGHQAFFT 300 EAAIAALKSGRLRGLAIDVYEQEASLFFQDLSSTIITDDVIQRLVSFPNVIVTGHQAFFT Sbjct: 241 EAAIAALKSGRLRGLAIDVYEQEASLFFQDLSSTIITDDVIQRLVSFPNVIVTGHQAFFT 300 Query: 301 EEAIGQIMQTTIENLSAFAAGDNLGERLVA 330 EEAIGQIMQTTIENLSAFAAGD+LGERLVA Sbjct: 301 EEAIGQIMQTTIENLSAFAAGDDLGERLVA 330 >gi|300310960|ref|YP_003775052.1| dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 326 Score = 131 bits (330), Expect = 7e-32, Method: Compositional matrix adjust. Identities = 97/294 (32%), Positives = 142/294 (48%), Gaps = 21/294 (7%) Query: 1 MKTAVFSARRYDQTLLARANAAHGHELVFLQDRLEAGTAQLAAGCQAVAAFVNDDLGGVV 60 MK +F AR +LAR E+ QD A+L A Q D L Sbjct: 1 MKPKIFVARAVFPEVLARLE--QHFEVESNQDDRIYSAAELQAKAQG-----KDGLFTTP 53 Query: 61 LEKL------AELGVRFVTTRSTGFNHIDVAAARALGLTVARVADYSPYSVAEFAVGLLQ 114 E + A ++ V + G+N+IDVAAA G+ D + A+F L+ Sbjct: 54 SEPVTAALFAANPQLKAVCNMAVGYNNIDVAAATQAGVMATNTPDVLNETTADFGWALMM 113 Query: 115 AVNRKIARASMRTREGNFE---LDGLMGVDLHGKTVGVIGTGKIGVLFARIMAGFGCQIL 171 A R+I + R G ++ D +G D+HG T+G+IG G+IG AR GF Q+L Sbjct: 114 AAARRITESEHFLRAGKWKKWSYDSFVGPDIHGATLGIIGMGRIGQAIARRSLGFDMQVL 173 Query: 172 GSDVHR-NPDFDALGG--RYVERTELFAASDVISLHCPLTEATRYLVNAQSLALAKPGCI 228 + R P+ +A RYV + EL +D + L P ++ T + + A LAL KP Sbjct: 174 YHNRSRLAPELEARANHARYVSKEELLRQADHVILVLPYSKDTHHTIAAAELALMKPTAT 233 Query: 229 LVNTSRGGLVDTEAAIAALKSGRLRGLAIDVYEQEASLF--FQDLSSTIITDDV 280 L N +RGG+VD A IAAL+ GR+ +DV+E E + F DLS+ ++T + Sbjct: 234 LTNIARGGIVDDAALIAALREGRIAAAGVDVFENEPAFKPEFLDLSNVVLTPHI 287 >gi|300313735|ref|YP_003777827.1| D-3-phosphoglycerate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 308 Score = 130 bits (326), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 77/195 (39%), Positives = 114/195 (58%), Gaps = 2/195 (1%) Query: 69 VRFVTTRSTGFNHIDVAAARALGLTVARVADYSPYSVAEFAVGLLQAVNRKIARASMRTR 128 +R ++ TG + ID AA+ G+ V A + +VAE L+ A + + R R Sbjct: 71 LRVISKHGTGIDSIDSQAAQQRGIAVKAAAGANAPAVAEHTWALIMACAKNVVGLDQRMR 130 Query: 129 EGNFELDGLMGVDLHGKTVGVIGTGKIGVLFARIMAGFGCQILGSDVHRNPDFDALGGRY 188 EG+++ ++L G+T+G++G G IG A + A G +L D + G + Sbjct: 131 EGHWDKSTHKSLELQGRTLGLVGLGAIGRRVAAVAAALGMPVLAHDPYAKEAPQ--GVQL 188 Query: 189 VERTELFAASDVISLHCPLTEATRYLVNAQSLALAKPGCILVNTSRGGLVDTEAAIAALK 248 V+ LFA SDV+SLHCPLT ++++NAQSLA K G ILVNT+RGGL+D +A IAAL Sbjct: 189 VDLDTLFAQSDVVSLHCPLTAENKHMINAQSLARMKDGAILVNTARGGLIDEQALIAALD 248 Query: 249 SGRLRGLAIDVYEQE 263 SG+LR +D +E+E Sbjct: 249 SGKLRAAGLDSFEKE 263 >gi|300311988|ref|YP_003776080.1| D-3-phosphoglycerate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 310 Score = 120 bits (301), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 94/285 (32%), Positives = 145/285 (50%), Gaps = 19/285 (6%) Query: 21 AAHGHELVFLQDRLEAGTAQLAAGCQAVAAFVNDDLGGVVLEKLAELG-VRFVTTRSTGF 79 AAH +++++ DR + +A + VA + + G+ E++ L ++ V T GF Sbjct: 20 AAH-YDILYAPDR-QRRDEMIAGPARKVAVVLTNGSTGLTAEEMRALPHLQLVCTLGAGF 77 Query: 80 NHIDVAAARALGLTVARVADYSPYSVAEFAVGLLQAVNRKIARASMRTREGNFELDGLMG 139 ++DVA A A G+ +A A + VA+ A+GLL A+ R I TR+G + + Sbjct: 78 ENVDVAHAEAHGIEIATGAGTNEDCVADHALGLLLAILRNIPVLDRYTRDGGWRETIPLQ 137 Query: 140 VDLHGKTVGVIGTGKIGVLFARIMAGFGCQILGSDVHRNPDFDALGGRYVERTELFAASD 199 L GK VG++G G IG AR A F +I + + D D + + +L +D Sbjct: 138 PQLAGKRVGIVGMGNIGKKIARRAAAFDAEIAYCNRKKRDDVDY--HYFPDVAQLAGWAD 195 Query: 200 VISLHCPLTEATRYLVNAQSLALAKPGCILVNTSRGGLVDTEAAIAALKSGRLRGLAIDV 259 + + P TR+L+NA+ L P LVN RG +VDT+A AAL SGRL G +DV Sbjct: 196 CLIVAAPGGAQTRHLINARVLEELGPQGYLVNIGRGSIVDTDALGAALSSGRLAGAGLDV 255 Query: 260 YEQEASLFFQDLSSTIITDDVIQRLVSFPNVIVTGHQAFFTEEAI 304 YE E Q ++ L++ PNV++T H A ++ EAI Sbjct: 256 YEGEP----QPPAA----------LIALPNVVLTPHIAGWSPEAI 286 >gi|300310832|ref|YP_003774924.1| D-isomer specific 2-hydroxyacid dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 319 Score = 109 bits (273), Expect = 3e-25, Method: Compositional matrix adjust. Identities = 83/265 (31%), Positives = 126/265 (47%), Gaps = 22/265 (8%) Query: 69 VRFVTTRSTGFNHIDVAAARALGLTVARVADYSPYSVAEFAVGLLQAVNRKIARASMRTR 128 ++ V+T S G ++ D+ R GL +A + A+ L+ A R++ + + Sbjct: 67 LKIVSTISVGVDNFDLDYFRQRGLMLAHTPGVLTEATADTIFALILASARRVVELAEYVK 126 Query: 129 EGNFEL---DGLMGVDLHGKTVGVIGTGKIGVLFAR-IMAGFGCQILGSDVHRNPDFDA- 183 G ++ + GV++HGKT+G+IG G+IG AR GFG IL + R PD +A Sbjct: 127 AGRWKGSIGEAQFGVNVHGKTLGLIGMGRIGSAVARRAHHGFGMPILYHN--RRPDPEAE 184 Query: 184 --LGGRYVERTELFAASDVISLHCPLTEATRYLVNAQSLALAKPGCILVNTSRGGLVDTE 241 LG RYV + +L A +D + + PLT T + A AL K I +N SRG +VD Sbjct: 185 RELGARYVSKEDLLAQADFVCVMLPLTPQTERMFGAPEFALMKRSAIFINASRGRIVDEA 244 Query: 242 AAIAALKSGRLRGLAIDVYEQEASLFFQDLSSTIITDDVIQRLVSFPNVIVTGHQAFFTE 301 A IAAL+ + G +DV+E E L Q L+ PNV+ H T Sbjct: 245 ALIAALQDKTIHGAGLDVFEVE-PLPLQ------------SPLLKLPNVVALPHIGSATH 291 Query: 302 EAIGQIMQTTIENLSAFAAGDNLGE 326 E + + + NL A G+ + Sbjct: 292 ETRLAMAELAVTNLIAGLRGEPVAH 316 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.136 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 863,122 Number of extensions: 31211 Number of successful extensions: 137 Number of sequences better than 1.0e-08: 9 Number of HSP's gapped: 126 Number of HSP's successfully gapped: 9 Length of query: 330 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 244 Effective length of database: 1,212,660 Effective search space: 295889040 Effective search space used: 295889040 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)