BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0082.p (428 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311827|ref|YP_003775919.1| sugar permease of the major fac... 857 0.0 gi|300310790|ref|YP_003774882.1| major facilitator superfamily p... 669 0.0 gi|300312423|ref|YP_003776515.1| proline/betaine transporter pro... 301 8e-83 gi|300309575|ref|YP_003773667.1| proline/betaine transporter pro... 296 3e-81 gi|300309357|ref|YP_003773449.1| major facilitator superfamily (... 285 4e-78 >gi|300311827|ref|YP_003775919.1| sugar permease of the major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 428 Score = 857 bits (2215), Expect = 0.0, Method: Compositional matrix adjust. Identities = 427/428 (99%), Positives = 427/428 (99%) Query: 1 MTTSTASQSKFSTVLRVTSGNFMEMFDFFLFGFYATHISKAFFPSDNEFASLMLTFMTFG 60 MTTSTASQSKFSTVLRVTSGNFMEMFDFFLFGFYATHISKAFFPSDNEFASLMLTFMTFG Sbjct: 1 MTTSTASQSKFSTVLRVTSGNFMEMFDFFLFGFYATHISKAFFPSDNEFASLMLTFMTFG 60 Query: 61 AGFLMRPLGAIILGAYVDRVGRRQGLIVTLALMALGTMLIAFVPSYATIGALAPLLVLIG 120 AGFLMRPLGAIILGAYVDRVGRRQGLIVTLALMALGTMLIAFVPSYATIGALAPLLVLIG Sbjct: 61 AGFLMRPLGAIILGAYVDRVGRRQGLIVTLALMALGTMLIAFVPSYATIGALAPLLVLIG 120 Query: 121 RLLQGFSAGVELGGVSVYLAEMATPGNKGFYVSWQSASQQVAIIVAAAIGYLLSTSLTPA 180 RLLQGFSAGVELGGVSVYLAEMATPGNKGFYVSWQSASQQVAIIVAAAIGYLLSTSLTPA Sbjct: 121 RLLQGFSAGVELGGVSVYLAEMATPGNKGFYVSWQSASQQVAIIVAAAIGYLLSTSLTPA 180 Query: 181 EVSGWGWRIPFFIGCLIVPVLFVIRRSLQETEEFMRRKHRPSTGQIFRSMIENWKLVIAG 240 EVSGWGWRIPFFIGCLIVPVLFVIRRSLQETEEFMRRKHRPSTGQIFRSMIENWKLVIAG Sbjct: 181 EVSGWGWRIPFFIGCLIVPVLFVIRRSLQETEEFMRRKHRPSTGQIFRSMIENWKLVIAG 240 Query: 241 MMLVSMTTVSFYLITVYTPTFGKNVLKLSTEASLIVTLCVGLSNFIWLPIMGALSDRVGR 300 MMLVSMTTVSFYLITVYTPTFGKNVLKLSTEASLIVTLCVGLSNFIWLPIMGALSDRVGR Sbjct: 241 MMLVSMTTVSFYLITVYTPTFGKNVLKLSTEASLIVTLCVGLSNFIWLPIMGALSDRVGR 300 Query: 301 RPLLVTFTVLTILTAYPAVNWLVHDASFAKMLIVELWLSFLYASYNGAMVVALTEVMPAD 360 RPLLVTFTVLTILTAYPAVNWLVHDASFAKMLIVELWLSFLYASYNGAMVVALTEVMPAD Sbjct: 301 RPLLVTFTVLTILTAYPAVNWLVHDASFAKMLIVELWLSFLYASYNGAMVVALTEVMPAD 360 Query: 361 VRTAGFSLAYSLATAVFGGFTPAIATGLIEYTHDKAAPGIWMSTAAVCGLIATLILYRNK 420 VRTAGFSLAYSLATAVFGGFTPAIATGLIEYTHDKAAPGIWMSTAAVCGLIATLILYRNK Sbjct: 361 VRTAGFSLAYSLATAVFGGFTPAIATGLIEYTHDKAAPGIWMSTAAVCGLIATLILYRNK 420 Query: 421 AARQAATA 428 A RQAATA Sbjct: 421 AVRQAATA 428 >gi|300310790|ref|YP_003774882.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 432 Score = 669 bits (1727), Expect = 0.0, Method: Compositional matrix adjust. Identities = 331/421 (78%), Positives = 373/421 (88%) Query: 2 TTSTASQSKFSTVLRVTSGNFMEMFDFFLFGFYATHISKAFFPSDNEFASLMLTFMTFGA 61 TT+ A SK STVLRVTSGNF+EMFDFFL+GFYA++ISK FFPSDNEF SL+LTF TFGA Sbjct: 4 TTTNAKHSKLSTVLRVTSGNFLEMFDFFLYGFYASYISKTFFPSDNEFVSLILTFGTFGA 63 Query: 62 GFLMRPLGAIILGAYVDRVGRRQGLIVTLALMALGTMLIAFVPSYATIGALAPLLVLIGR 121 GFLMRPLGAIILG+Y+DRVGRRQGLIVTLA+MA+GT+LIAFVP YATIG AP+LVL GR Sbjct: 64 GFLMRPLGAIILGSYIDRVGRRQGLIVTLAIMAIGTLLIAFVPGYATIGVAAPILVLTGR 123 Query: 122 LLQGFSAGVELGGVSVYLAEMATPGNKGFYVSWQSASQQVAIIVAAAIGYLLSTSLTPAE 181 LLQGFSAGVELGGVSVYLAEMATPGNKGFYVSWQSASQQ AI+ +A IGYL+S SL+P + Sbjct: 124 LLQGFSAGVELGGVSVYLAEMATPGNKGFYVSWQSASQQAAIMASALIGYLVSQSLSPVQ 183 Query: 182 VSGWGWRIPFFIGCLIVPVLFVIRRSLQETEEFMRRKHRPSTGQIFRSMIENWKLVIAGM 241 +S WGWRIPFFIGC+IVP +F+IRRSLQET+EF+ RK PS G+I S+ +NWK+V+AGM Sbjct: 184 ISDWGWRIPFFIGCMIVPAIFIIRRSLQETDEFLARKRHPSFGEIMSSIAQNWKIVVAGM 243 Query: 242 MLVSMTTVSFYLITVYTPTFGKNVLKLSTEASLIVTLCVGLSNFIWLPIMGALSDRVGRR 301 M+V MTTVSFYLITVYTPTFGKNVLKLS SLIVT CVGLSNF WLPIMGALSDRVGRR Sbjct: 244 MMVVMTTVSFYLITVYTPTFGKNVLKLSASDSLIVTFCVGLSNFCWLPIMGALSDRVGRR 303 Query: 302 PLLVTFTVLTILTAYPAVNWLVHDASFAKMLIVELWLSFLYASYNGAMVVALTEVMPADV 361 P+LV FT+LT+LTAYP ++WLV D SF +LI ELWLSFLYASYNGA VVALTEVMPA V Sbjct: 304 PVLVAFTLLTLLTAYPVMSWLVSDISFKNLLITELWLSFLYASYNGAAVVALTEVMPAHV 363 Query: 362 RTAGFSLAYSLATAVFGGFTPAIATGLIEYTHDKAAPGIWMSTAAVCGLIATLILYRNKA 421 RT GFSLAYSLATA+FGGFTP ++T LIE + DKAAPG WMS AAVCGL+ATL+LYR K+ Sbjct: 364 RTVGFSLAYSLATAIFGGFTPLVSTWLIETSGDKAAPGYWMSFAAVCGLLATLVLYRGKS 423 Query: 422 A 422 A Sbjct: 424 A 424 >gi|300312423|ref|YP_003776515.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 427 Score = 301 bits (770), Expect = 8e-83, Method: Compositional matrix adjust. Identities = 165/396 (41%), Positives = 241/396 (60%), Gaps = 5/396 (1%) Query: 20 GNFMEMFDFFLFGFYATHISKAFFPSDNEFASLMLTFMTFGAGFLMRPLGAIILGAYVDR 79 GN +E FD ++G +A I+K FFP+ ++ SL+LTF TFG F MRPLGA++LGAY DR Sbjct: 27 GNALEWFDILIYGAFAVVIAKQFFPTGDDSVSLLLTFATFGVSFFMRPLGAVVLGAYSDR 86 Query: 80 VGRRQGLIVTLALMALGTMLIAFVPSYATIGALAPLLVLIGRLLQGFSAGVELGGVSVYL 139 GR+ L++++ LM +GT +IAF+PSYA+IG LAP + +G+++QGFSAG E G + +L Sbjct: 87 AGRKAALMLSIMLMTVGTAMIAFMPSYASIGLLAPAGIALGKMIQGFSAGGEFGSSTAFL 146 Query: 140 AEMATPGNKGFYVSWQSASQQVAIIVAAAIGYLLSTSLTPAEVSGWGWRIPFFIGCLIVP 199 E A P +GF+ SWQ ASQ +++++AA G +L+ LTP +++ WGWR+PF G LI P Sbjct: 147 VEHA-PHRRGFFSSWQVASQGISLLLAALFGAVLNNMLTPEQLASWGWRVPFIFGLLIAP 205 Query: 200 VLFVIRRSLQETEEFMRRKHRPSTGQIFRSMIENWKL-VIAGMMLVSMTTVSFYLITVYT 258 IRR L E EF + T R + K+ ++ G V M TVS YL ++Y Sbjct: 206 AGIYIRRHLDEAPEFKESSEK--TNAPLRDTFAHQKMRLLIGAGSVIMATVSVYL-SLYI 262 Query: 259 PTFGKNVLKLSTEASLIVTLCVGLSNFIWLPIMGALSDRVGRRPLLVTFTVLTILTAYPA 318 PT+ L L +S GL FI P++GALSD++GR P ++T + L I+ YP Sbjct: 263 PTYAVKQLGLPAWSSFAAMSVAGLIMFIGSPLVGALSDKIGRTPFMITSSALYIVLTYPM 322 Query: 319 VNWLVHDASFAKMLIVELWLSFLYASYNGAMVVALTEVMPADVRTAGFSLAYSLATAVFG 378 +L + F ++L+++ + L Y AM L ++ P R G SLAY++A +FG Sbjct: 323 FVFLTNSPGFLQLLLLQTVIGVLMTMYFAAMPALLADIFPVATRGTGMSLAYNIAVTIFG 382 Query: 379 GFTPAIATGLIEYTHDKAAPGIWMSTAAVCGLIATL 414 GF I T LI++T DK + ++ AV LIA+L Sbjct: 383 GFAGLIITWLIDFTGDKLSVSYFVIFGAVLSLIASL 418 >gi|300309575|ref|YP_003773667.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 433 Score = 296 bits (757), Expect = 3e-81, Method: Compositional matrix adjust. Identities = 163/411 (39%), Positives = 241/411 (58%), Gaps = 3/411 (0%) Query: 4 STASQSKFSTVLRVTSGNFMEMFDFFLFGFYATHISKAFFPSDNEFASLMLTFMTFGAGF 63 S ++Q + ++ GN +E FDF ++ F+A I+K FFP+ N+ SL+L TFG GF Sbjct: 13 SVSNQQRRRAIVATVIGNGLEWFDFTVYSFFAVIIAKLFFPTGNDLTSLLLAVATFGVGF 72 Query: 64 LMRPLGAIILGAYVDRVGRRQGLIVTLALMALGTMLIAFVPSYATIGALAPLLVLIGRLL 123 MRP+G I+LG Y DRVGR+ L VT+ LMA+GT +I P+Y +IG APL++++ RLL Sbjct: 73 FMRPVGGILLGIYADRVGRKAALSVTILLMAVGTTMIGLAPTYESIGLFAPLIIVVARLL 132 Query: 124 QGFSAGVELGGVSVYLAEMATPGNKGFYVSWQSASQQVAIIVAAAIGYLLSTSLTPAEVS 183 QGFSAG E+G + +L E A + +Y SW AS VA+++ AA+G ++ SL A ++ Sbjct: 133 QGFSAGGEMGSATAFLTEYAPERERAYYSSWIQASIGVAVLLGAAVGTFVTASLDAAALA 192 Query: 184 GWGWRIPFFIGCLIVPVLFVIRRSLQETEEFMRRKHRPSTGQIFRSMIENW-KLVIAGMM 242 WGWR+PF +G +I PV + IR L+ET F+ K + T + +I N+ + A Sbjct: 193 SWGWRLPFLLGIIIGPVGYYIRHHLEETPTFLEEKDK--TDSPLKEVIRNFPRETSASFS 250 Query: 243 LVSMTTVSFYLITVYTPTFGKNVLKLSTEASLIVTLCVGLSNFIWLPIMGALSDRVGRRP 302 +V + TV Y++ Y PT+ VLKL I + GL + PI+G L+DR+GRRP Sbjct: 251 MVILWTVCTYVLLFYMPTYSVKVLKLPQTTGFIAGMVGGLCIMVLAPIVGRLADRIGRRP 310 Query: 303 LLVTFTVLTILTAYPAVNWLVHDASFAKMLIVELWLSFLYASYNGAMVVALTEVMPADVR 362 LL L ++ AYP ++ S + +LI +L L A+Y G ++ A +E+ PA V Sbjct: 311 LLSGSAALILMLAYPMFAYINSAPSLSTLLIFQLVFGTLIAAYTGPILAAFSELFPAKVL 370 Query: 363 TAGFSLAYSLATAVFGGFTPAIATGLIEYTHDKAAPGIWMSTAAVCGLIAT 413 + G S+AY+LA +FGGF T LI T AP ++ AA + T Sbjct: 371 STGLSVAYNLAVTIFGGFASFFITWLIAATGSTMAPAFYVMIAAAISFVGT 421 >gi|300309357|ref|YP_003773449.1| major facilitator superfamily (MFS) transporter protein [Herbaspirillum seropedicae SmR1] Length = 437 Score = 285 bits (729), Expect = 4e-78, Method: Compositional matrix adjust. Identities = 151/407 (37%), Positives = 240/407 (58%), Gaps = 4/407 (0%) Query: 9 SKFSTVLRVTSGNFMEMFDFFLFGFYATHISKAFFPSDNEFASLMLTFMTFGAGFLMRPL 68 +K V+ GN +E +DF ++GF++ I++ FFP+DNE+ SL++ TFG GF MRP+ Sbjct: 6 NKQRQVIAAIVGNALEWYDFIVYGFFSAIIARLFFPADNEYTSLLVALATFGIGFFMRPV 65 Query: 69 GAIILGAYVDRVGRRQGLIVTLALMALGTMLIAFVPSYATIGALAPLLVLIGRLLQGFSA 128 G ++LG Y DR GR+ + V + LM L +IAF P+YATIG AP+++++ R+LQGF+ Sbjct: 66 GGVLLGLYADRKGRKAAMQVIIVLMTLAIAIIAFTPTYATIGIAAPIMIVVARMLQGFAT 125 Query: 129 GVELGGVSVYLAEMATPGNKGFYVSWQSASQQVAIIVAAAIGYLLSTSLTPAEVSGWGWR 188 G E + +L E A +G Y SWQ Q +A+ A +G L++ +L+P + WGWR Sbjct: 126 GGEYSSSTAFLVESAPAHKRGLYGSWQLVGQCLAVFCGAGMGALVTRNLSPEALDSWGWR 185 Query: 189 IPFFIGCLIVPVLFVIRRSLQETEEFMRRKHRPSTG--QIFRSMIENWKLVIAGMMLVSM 246 +PF +G LI PV IRR + ET++F+ ++P+ ++ + N + V+ M V Sbjct: 186 LPFVLGLLIGPVGLWIRRHMHETDDFLESANKPNAAPIKLLDVVKTNLRGVLVSMGQVIN 245 Query: 247 TTVSFYLITVYTPTFGKNVLKLSTEASLIVTL-CVGLSNFIWLPIMGALSDRVGRRPLLV 305 TV+FY++ V PTF L L + + +V + V L + +P GALSDR+GRR +L+ Sbjct: 246 GTVAFYVVLVNMPTFANKQLGLPLDQAFMVQMIAVALLTAV-IPFAGALSDRLGRRTVLL 304 Query: 306 TFTVLTILTAYPAVNWLVHDASFAKMLIVELWLSFLYASYNGAMVVALTEVMPADVRTAG 365 ++ +L YP W+ S ++L++++ L + + G M AL+E P VR+ G Sbjct: 305 YSSLAFLLMVYPLFVWVSAAPSLPRLLVMQVLLCIVIGANFGPMPTALSEQFPTRVRSTG 364 Query: 366 FSLAYSLATAVFGGFTPAIATGLIEYTHDKAAPGIWMSTAAVCGLIA 412 ++AY+LA +FGGF P I T L + + AP ++ AA G+ A Sbjct: 365 LAVAYNLAAMLFGGFAPFIVTWLTKTSGSPVAPAWYVLFAACIGVSA 411 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.138 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,168,049 Number of extensions: 45686 Number of successful extensions: 334 Number of sequences better than 1.0e-08: 15 Number of HSP's gapped: 304 Number of HSP's successfully gapped: 15 Length of query: 428 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 340 Effective length of database: 1,203,190 Effective search space: 409084600 Effective search space used: 409084600 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)