BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0070.p (289 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311840|ref|YP_003775932.1| nitrate/sulfonate/bicarbonate A... 568 e-163 gi|300309401|ref|YP_003773493.1| nitrate/sulfonate/bicarbonate A... 335 2e-93 gi|300309716|ref|YP_003773808.1| nitrate/sulfonate/bicarbonate A... 95 7e-21 gi|300310979|ref|YP_003775071.1| alkanesulfonates ABC transporte... 90 2e-19 gi|300309985|ref|YP_003774077.1| nitrate/sulfonate/bicarbonate A... 84 1e-17 >gi|300311840|ref|YP_003775932.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 289 Score = 568 bits (1465), Expect = e-163, Method: Compositional matrix adjust. Identities = 289/289 (100%), Positives = 289/289 (100%) Query: 1 MHTNLPFVRPEILVDPNPHATAEVQRPLSAFESLYRMGWLRKTVILLVLAAIWEVYGRWL 60 MHTNLPFVRPEILVDPNPHATAEVQRPLSAFESLYRMGWLRKTVILLVLAAIWEVYGRWL Sbjct: 1 MHTNLPFVRPEILVDPNPHATAEVQRPLSAFESLYRMGWLRKTVILLVLAAIWEVYGRWL 60 Query: 61 DNALLFPSFSETVQAFLSGIGSGVLLQRAAVSMQTLLVGYGLGILLAGLLTTVAISSRIG 120 DNALLFPSFSETVQAFLSGIGSGVLLQRAAVSMQTLLVGYGLGILLAGLLTTVAISSRIG Sbjct: 61 DNALLFPSFSETVQAFLSGIGSGVLLQRAAVSMQTLLVGYGLGILLAGLLTTVAISSRIG 120 Query: 121 NDLLETLTSMFNPLPAIALLPLALIWFGLGTGSIIFVLVHSVLWAVALNTHGGFRAVSNT 180 NDLLETLTSMFNPLPAIALLPLALIWFGLGTGSIIFVLVHSVLWAVALNTHGGFRAVSNT Sbjct: 121 NDLLETLTSMFNPLPAIALLPLALIWFGLGTGSIIFVLVHSVLWAVALNTHGGFRAVSNT 180 Query: 181 LRMVGQNYGLRRFSLVKSILIPAAFPSILTGLKVGWAFAWRTLIAAELVFGVSSGAGGLG 240 LRMVGQNYGLRRFSLVKSILIPAAFPSILTGLKVGWAFAWRTLIAAELVFGVSSGAGGLG Sbjct: 181 LRMVGQNYGLRRFSLVKSILIPAAFPSILTGLKVGWAFAWRTLIAAELVFGVSSGAGGLG 240 Query: 241 WYIFENRNQLETANVFSGLFFVIIIGLVVENLIFAQIEKRTIRRWGMQH 289 WYIFENRNQLETANVFSGLFFVIIIGLVVENLIFAQIEKRTIRRWGMQH Sbjct: 241 WYIFENRNQLETANVFSGLFFVIIIGLVVENLIFAQIEKRTIRRWGMQH 289 >gi|300309401|ref|YP_003773493.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 288 Score = 335 bits (860), Expect = 2e-93, Method: Compositional matrix adjust. Identities = 169/284 (59%), Positives = 214/284 (75%) Query: 6 PFVRPEILVDPNPHATAEVQRPLSAFESLYRMGWLRKTVILLVLAAIWEVYGRWLDNALL 65 P +R E + P +QR S + L++ GW+RK ILL+LA +WE RW +N LL Sbjct: 5 PPIRDEYEIPLTPLPGLVLQRQFSLPQRLWQQGWVRKGAILLLLALVWEGVARWQNNDLL 64 Query: 66 FPSFSETVQAFLSGIGSGVLLQRAAVSMQTLLVGYGLGILLAGLLTTVAISSRIGNDLLE 125 PSF +T QAF+ G+ SG L + +S+Q LL Y LGI+ A LTT+A+S++ G DLL Sbjct: 65 LPSFLQTAQAFIDGLRSGELFGKTWLSLQVLLKAYVLGIVAAFALTTLAVSTQFGRDLLG 124 Query: 126 TLTSMFNPLPAIALLPLALIWFGLGTGSIIFVLVHSVLWAVALNTHGGFRAVSNTLRMVG 185 TLTSMFNPLPAIALLP+AL+WFGLG GS+IFVLVHSVLWA+A+NT+ GF VS+TLRM G Sbjct: 125 TLTSMFNPLPAIALLPIALLWFGLGQGSLIFVLVHSVLWALAVNTYAGFLGVSDTLRMAG 184 Query: 186 QNYGLRRFSLVKSILIPAAFPSILTGLKVGWAFAWRTLIAAELVFGVSSGAGGLGWYIFE 245 +NYGLR V +L+PAA PSIL GL++GWAFAWRTLIAAELVFG +SG GGLGWYIF+ Sbjct: 185 RNYGLRGLGFVLQVLVPAALPSILAGLRIGWAFAWRTLIAAELVFGATSGKGGLGWYIFQ 244 Query: 246 NRNQLETANVFSGLFFVIIIGLVVENLIFAQIEKRTIRRWGMQH 289 NRN+L T VF+GL VI+IGL+VENL+F +E+ T+++WGMQ Sbjct: 245 NRNELYTDKVFAGLAMVILIGLLVENLVFNTLERVTVKKWGMQR 288 >gi|300309716|ref|YP_003773808.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 273 Score = 95.1 bits (235), Expect = 7e-21, Method: Compositional matrix adjust. Identities = 66/243 (27%), Positives = 125/243 (51%), Gaps = 8/243 (3%) Query: 28 LSAFESLYRMGWLRKTVILLVLAAIWEVYGRWLDNALLFPSFSETVQAFLSGIGSGVLLQ 87 ++ +E R GW+ + L + W V +W++ A + ++L+ SG L+ Sbjct: 20 IALWEGASRAGWINPQI----LPSPWAVLRKWVEYATPLKPYDPEAGSWLAWAFSGELVM 75 Query: 88 RAAVSMQTLLVGYGLGILLAGLLTTVAISSRIGNDLLETLTSMFNPLPAIALLPLALIWF 147 A S+ ++ G+ +G LA L + SS+ L+ + P+P IA +PLA++WF Sbjct: 76 DAIGSLYRVVAGFLIGAGLALPLGLLMGSSQRIYGLMNLTVQVIRPIPPIAYIPLAILWF 135 Query: 148 GLGTGSIIFVLVHSVLWAVALNTHGGFRAVSNTLRMVGQNYGLRRFSLVKSILIPAAFPS 207 GLG +F++ + V +NT G R V + +N G + +L +++PAA P Sbjct: 136 GLGNPPALFLISLGAFFPVLMNTIAGVRQVDSIYLRAARNLGASQSTLFLRVMLPAAVPY 195 Query: 208 ILTGLKVGWAFAWRTLIAAELVFGVSSGAGGLGWYIFENRNQLETANVFSGLFFVIIIGL 267 IL+G+++G A+ +I AE++ + + GLG+ I E R + + +G+ + ++GL Sbjct: 196 ILSGVRIGIGTAFIVVIVAEMI----AVSNGLGFRIMEAREYFWSDKIIAGMITIGLLGL 251 Query: 268 VVE 270 ++ Sbjct: 252 AID 254 >gi|300310979|ref|YP_003775071.1| alkanesulfonates ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 279 Score = 90.1 bits (222), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 68/221 (30%), Positives = 111/221 (50%), Gaps = 7/221 (3%) Query: 53 WEVYGR--WLDNALLFPSFSETVQAFLSGIGSGVLLQRAAVSMQTLLVGYGLGILLAGLL 110 WEV + WL + +L P ++A SG L + AVS G+ +G L +L Sbjct: 47 WEVAAQAGWLSSRIL-PEPLAVLKAAWELSASGELWRHLAVSSGRAASGFAVGAGLGLIL 105 Query: 111 TTVAISSRIGNDLLETLTSMFNPLPAIALLPLALIWFGLGTGSIIFVLVHSVLWAVALNT 170 ++ S R LL+T M +PA+AL+PL ++WFG+ + +F++ V + V LNT Sbjct: 106 GLLSGSLRSAELLLDTTLQMVRNIPALALIPLVILWFGIDETAKLFLVSVGVFFPVYLNT 165 Query: 171 HGGFRAVSNTLRMVGQNYGLRRFSLVKSILIPAAFPSILTGLKVGWAFAWRTLIAAELVF 230 G R+V L + ++YGL + L + +++P A P+IL GL+ W LI AE + Sbjct: 166 FHGIRSVDQGLIEMAKSYGLSGWPLYRDVILPGALPAILVGLRFALGLVWVLLIVAETI- 224 Query: 231 GVSSGAGGLGWYIFENRNQLETANVFSGLFFVIIIGLVVEN 271 S G+G+ R L+T V G+ ++G + + Sbjct: 225 ---SAQAGIGYMTMNAREFLQTDVVLVGILLYALLGKLADT 262 >gi|300309985|ref|YP_003774077.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 278 Score = 84.0 bits (206), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 77/279 (27%), Positives = 122/279 (43%), Gaps = 9/279 (3%) Query: 16 PNPHATAEVQRPLSAFESLYRMGWLRKTVILLVLAAIWEVYGRW-LDNALLFPSFSETVQ 74 P +TA R + YR G ++ L + E R L A L P SE Sbjct: 2 PTSTSTARTHRRGWPHDDAYRPGRFTGWIVPLAFLLLVEAGARLELIPARLLPPPSELAH 61 Query: 75 AFLSGIGSGVLLQRAAVSMQTLLVGYGLGILLAGLLTTVAISSRIGNDLLETLTSMFNPL 134 S G LL A S+ + G+ +G LA L + SR LLE + Sbjct: 62 TLWSLAQRGELLPHIAASLARVAAGFVIGSALAVALGLLVGISRRAEALLEPSFQALRAI 121 Query: 135 PAIALLPLALIWFGLGTGSIIFVLVHSVLWAVALNTHGGFRAVSNTLRMVGQNYGLRRFS 194 P++A +PL L+W G+ + ++ + V LN G R + + L VG GL + Sbjct: 122 PSLAWIPLLLLWLGIDETPKVVLIAIGAFFPVYLNLVAGIRDIDHKLIEVGTTSGLNTQA 181 Query: 195 LVKSILIPAAFPSILTGLKVGWAFAWRTLIAAELVFGVSSGAGGLGWYIFENRNQLETAN 254 L+ IL+PA+ P + GL+ G + AW L+AAEL+ + G+G+ + + R Sbjct: 182 LIGHILLPASLPYLFAGLRGGLSLAWMFLVAAELI----AATKGVGYLLSDGRESSRPDL 237 Query: 255 VFSGLFFVIIIGLVVENLIFAQIEK----RTIRRWGMQH 289 V + + + ++G + + L+ A +K R RR Q Sbjct: 238 VLAAILILALLGKLSDGLLRAVEQKSLGWREARRQSSQQ 276 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.141 0.435 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 793,156 Number of extensions: 29436 Number of successful extensions: 158 Number of sequences better than 1.0e-08: 15 Number of HSP's gapped: 153 Number of HSP's successfully gapped: 15 Length of query: 289 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 205 Effective length of database: 1,222,130 Effective search space: 250536650 Effective search space used: 250536650 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 130 (54.7 bits)