BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0057.p (309 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311849|ref|YP_003775941.1| LysR family transcription regul... 618 e-178 gi|300312870|ref|YP_003776962.1| transcription regulator protein... 113 2e-26 gi|300313810|ref|YP_003777902.1| LysR family transcription regul... 93 3e-20 gi|300312305|ref|YP_003776397.1| LysR family transcription regul... 78 9e-16 gi|300312244|ref|YP_003776336.1| LysR family transcription regul... 76 4e-15 >gi|300311849|ref|YP_003775941.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 309 Score = 618 bits (1593), Expect = e-178, Method: Compositional matrix adjust. Identities = 309/309 (100%), Positives = 309/309 (100%) Query: 1 MNIERLSLDQLRVFVQVADSGSFLAAARKLARAQSAVSYAIASLEEQLGLLLFDRSGYRP 60 MNIERLSLDQLRVFVQVADSGSFLAAARKLARAQSAVSYAIASLEEQLGLLLFDRSGYRP Sbjct: 1 MNIERLSLDQLRVFVQVADSGSFLAAARKLARAQSAVSYAIASLEEQLGLLLFDRSGYRP 60 Query: 61 QLTAPGEALLADARQVLDHVDSLQVRASSYARGQELEMALAVDVFFPLDCLVDLLQRFRA 120 QLTAPGEALLADARQVLDHVDSLQVRASSYARGQELEMALAVDVFFPLDCLVDLLQRFRA Sbjct: 61 QLTAPGEALLADARQVLDHVDSLQVRASSYARGQELEMALAVDVFFPLDCLVDLLQRFRA 120 Query: 121 AFPAVTVRLEVEALGAVAERVIDGRAMLGILGTLPTTPPNLMRITLPSVRLVAVVAPHHP 180 AFPAVTVRLEVEALGAVAERVIDGRAMLGILGTLPTTPPNLMRITLPSVRLVAVVAPHHP Sbjct: 121 AFPAVTVRLEVEALGAVAERVIDGRAMLGILGTLPTTPPNLMRITLPSVRLVAVVAPHHP 180 Query: 181 LAKVKGRIAEKMLLQQTQLVLSDRSELTARQDFSVHSRLTWRMSDLGTKHALLRAGMGWG 240 LAKVKGRIAEKMLLQQTQLVLSDRSELTARQDFSVHSRLTWRMSDLGTKHALLRAGMGWG Sbjct: 181 LAKVKGRIAEKMLLQQTQLVLSDRSELTARQDFSVHSRLTWRMSDLGTKHALLRAGMGWG 240 Query: 241 NMPLHVVKEDLAAGRLVSISTIHHPPQGAELPIQCVYKPDHRAGPALSWWLDALANLGSL 300 NMPLHVVKEDLAAGRLVSISTIHHPPQGAELPIQCVYKPDHRAGPALSWWLDALANLGSL Sbjct: 241 NMPLHVVKEDLAAGRLVSISTIHHPPQGAELPIQCVYKPDHRAGPALSWWLDALANLGSL 300 Query: 301 EAQPAAGSA 309 EAQPAAGSA Sbjct: 301 EAQPAAGSA 309 >gi|300312870|ref|YP_003776962.1| transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 312 Score = 113 bits (282), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 90/300 (30%), Positives = 146/300 (48%), Gaps = 14/300 (4%) Query: 5 RLSLDQLRVFVQVADSGSFLAAARKLARAQSAVSYAIASLEEQLGLLLFDRSGYRPQLTA 64 L+L+ L + + GSF AAA L R SA++Y++ LE+ L +LLFDR G+R QLT Sbjct: 2 NLTLESLLILDMIDRKGSFAAAAVALDRVPSALTYSVRKLEDDLDVLLFDRRGHRAQLTP 61 Query: 65 PGEALLADARQVLDHVDSLQVRASSYARGQELEMALAVDVFFPLDCLVDLLQRFRAAFPA 124 G LL + R +L + L+ R A G+E E+ + ++ P D ++ ++ F Sbjct: 62 AGLQLLQEGRHLLLAANDLEQRVKRTATGRETELHIVLNSLIPFDKMLPIIAAFDREQSG 121 Query: 125 VTVRLEVEALGAVAERVIDGRAMLGILGTLPTTPPNLMRIT-------LPSVRLVAVVAP 177 +R L E++I+GRA L I TL P ++R + L +V V VVAP Sbjct: 122 TRLRFTPGVLTGAWEKLIEGRANLVIGVTLDG--PEVVRTSGRFQMQELGAVDWVFVVAP 179 Query: 178 HHPLAKVKGRIAEKMLLQQTQLVLSDRSELTARQDFSVHS-RLTWRMSDLGTKHALLRAG 236 HPLA V+ ++ +++ + + D S+ + S + T ++ + K AG Sbjct: 180 QHPLAGVEEPLSAELVRSHRAIAVGDNSQSLPTLTMGLLSGQETLTVATVADKLQAQLAG 239 Query: 237 MGWGNMPLHVVKEDLAAGRLVSISTIHHPPQGAELPIQCVYKPDHRAGPALSWWLDALAN 296 +G G++P + LA+G LV T+ P + V P G +L W++ LA Sbjct: 240 LGCGHLPRIWAEPYLASGALVEKQTLAAKPNDNFM----VAWPKAEQGKSLKWFIQHLAQ 295 >gi|300313810|ref|YP_003777902.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 301 Score = 92.8 bits (229), Expect = 3e-20, Method: Compositional matrix adjust. Identities = 86/259 (33%), Positives = 124/259 (47%), Gaps = 10/259 (3%) Query: 6 LSLDQLRVFVQVADSGSFLAAARKLARAQSAVSYAIASLEEQLGLLLFDRSGYRPQLTAP 65 S D +RVF+ V DSGSF AAAR+L R S+VS I+ LE +L L LFDR+ + T Sbjct: 3 FSSDNVRVFLAVLDSGSFSAAARELGRVPSSVSMTISQLEAELDLQLFDRTARDARPTDM 62 Query: 66 GEALLADARQVLDHVDSLQVRASSYARGQELEMALAVDVFFPLDCLVDLLQRFRAAFPAV 125 AL DAR + + L A + +G E ++ LAV L FP+ Sbjct: 63 ARALEPDARLLASTLRQLDAHALAMHQGLERKLTLAVAPELLSAPWAKPLAVLAQEFPS- 121 Query: 126 TVRLEVEALG---AVAERVI-DGRAMLGILGTLPTTPPNLMRITLPSVRLVAVVAPHHP- 180 LEVE L A A R++ D A L ++ P S LVAV+AP HP Sbjct: 122 ---LEVEVLSSPQADAMRMLYDDTAQLALVFERPAGNDREAFQEFSSEMLVAVIAPDHPA 178 Query: 181 LAKVKGRIAEKMLLQQTQLVLSDRSELTARQDFSVHSRLTWRMSDLGTKHALLRAGMGWG 240 L + K ++ + L Q+ ++ R + F + +R WR + +L+R G+GW Sbjct: 179 LRRKKRKLRYEDLYDIRQIAVASRDARVSDPRFFL-ARQIWRTDNHLATLSLVREGLGWA 237 Query: 241 NMPLHVVKEDLAAGRLVSI 259 +P +V+ + AG L I Sbjct: 238 YLPQSLVQAQIEAGALTVI 256 >gi|300312305|ref|YP_003776397.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 297 Score = 78.2 bits (191), Expect = 9e-16, Method: Compositional matrix adjust. Identities = 70/225 (31%), Positives = 108/225 (48%), Gaps = 9/225 (4%) Query: 4 ERLSLDQLRVFVQVADSGSFLAAARKLARAQSAVSYAIASLEEQLGLLLFDRSGYRPQLT 63 R L LR+F+ + +SGS A++ A ++ S I ++E+ LG L +R Q T Sbjct: 3 NRFELSDLRLFLHIVESGSITGGAQRAHMALASASARIRNMEDALGTPLLERGRRGVQTT 62 Query: 64 APGEALLADARQVLDHVDSLQVRASSYARGQELEMALAVDVFFPLDCLVDLLQRFRAAFP 123 G AL AR + ++ L+ YARG + + L + + L ++L RF A P Sbjct: 63 DAGRALAHHARMMAQQMEQLRDELGQYARGLKGHIRLLCNTSAMTEFLPEVLARFLARHP 122 Query: 124 AVTVRLEVEALGAVAERVIDGRAMLGILGTLPTTPPNLMRITLPSVRLVAVVAPHHPLAK 183 + LE + V + VIDG A +GI+ + P L LVAV++ HPLA+ Sbjct: 123 HTDIELEEKLSYEVVQAVIDGVADVGIIADSTDSGP-LQTYPFRQDHLVAVLSKRHPLAR 181 Query: 184 VKGRIAEKMLLQQTQLVLSDRSELTARQDFSVHS-----RLTWRM 223 K +A LL + + LS S L +Q S H+ RL +R+ Sbjct: 182 RKS-LAFAELLGEDFVGLSGDSAL--QQHLSGHATRAGKRLQYRV 223 >gi|300312244|ref|YP_003776336.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 293 Score = 75.9 bits (185), Expect = 4e-15, Method: Compositional matrix adjust. Identities = 89/299 (29%), Positives = 134/299 (44%), Gaps = 22/299 (7%) Query: 6 LSLDQLRVFVQVADSGSFLAAARKLARAQSAVSYAIASLEEQLGLLLFDRSGYRPQLTAP 65 L ++LR FV V ++G+ AAA LA+ S VS A++ LE++LG L R+ R +LT Sbjct: 3 LQSEELRTFVAVVEAGTLSAAAELLAQTTSGVSRALSRLEDKLGASLLTRTTRRMELTEE 62 Query: 66 GEALLADARQVLDHVDSLQVRASSYARGQELEMALAVDVFFPLDCLVDLLQRFRAAFPAV 125 G A L AR +L ++S Q R ++ + F L C+V + +FRAA+P + Sbjct: 63 GRAFLEQARAILAAMESAQDSIRLRTRKPVGKLRVDASAPFMLHCVVPHVGQFRAAYPDI 122 Query: 126 TVRLEVEALGAVAERVID---GRAMLGI-LGTLPTTPPNLMRITLPSVRLVAVVAPHHPL 181 + L +R ID RA + I +G L + +L L S RL V +P + L Sbjct: 123 ELELTTN------DRFIDLVEHRADIAIRIGELQDS--SLHARALSSARLNVVASPAY-L 173 Query: 182 AKVKGRIAEKMLLQQTQLVLSDRSE------LTARQDFSVHSRLTWRMSDLGTKHALLRA 235 A+ G L+Q QL+ + + L +R R S T L Sbjct: 174 AR-HGEPESVEALEQHQLIGFSQPDQLNHWPLRHSAGDRYQTRPAIRASSGETIRHLALQ 232 Query: 236 GMGWGNMPLHVVKEDLAAGRLVSISTIHHPPQGAELPIQCVYKPDHRAGPALSWWLDAL 294 G G + + D+AAGRLV + + G I VY + +S +LD L Sbjct: 233 GQGIACLSNFMSDADIAAGRLVRL--LPQADSGYRQKISAVYYYSTQLSRRISCFLDFL 289 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.135 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 963,377 Number of extensions: 40189 Number of successful extensions: 255 Number of sequences better than 1.0e-08: 49 Number of HSP's gapped: 238 Number of HSP's successfully gapped: 49 Length of query: 309 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 224 Effective length of database: 1,217,395 Effective search space: 272696480 Effective search space used: 272696480 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)