BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0047.p (287 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311859|ref|YP_003775951.1| CobW/P47K family protein [Herba... 442 e-125 gi|300310263|ref|YP_003774355.1| cobalamin synthesis P47K protei... 233 1e-62 gi|300314043|ref|YP_003778135.1| cobalamin synthesis protein/P47... 122 4e-29 gi|300311960|ref|YP_003776052.1| G3E family GTPase [Herbaspirill... 84 1e-17 >gi|300311859|ref|YP_003775951.1| CobW/P47K family protein [Herbaspirillum seropedicae SmR1] Length = 404 Score = 442 bits (1136), Expect = e-125, Method: Compositional matrix adjust. Identities = 220/221 (99%), Positives = 221/221 (100%) Query: 5 MTSSQRQAADRRLPVTVLSGFLGAGKTTLLNHILHNREGRRVAVIVNDMSEVNIDAALVR 64 MTSSQRQAADRRLPVTVLSGFLGAGKTTLLNHILHNREGRRVAVIVNDMSEVNIDAALVR Sbjct: 1 MTSSQRQAADRRLPVTVLSGFLGAGKTTLLNHILHNREGRRVAVIVNDMSEVNIDAALVR 60 Query: 65 DGGADLSRTEEKLVEMSNGCICCTLREDLLMEVRKLARERRFDQLVIESTGISEPLPVAE 124 DGGADL+RTEEKLVEMSNGCICCTLREDLLMEVRKLARERRFDQLVIESTGISEPLPVAE Sbjct: 61 DGGADLARTEEKLVEMSNGCICCTLREDLLMEVRKLARERRFDQLVIESTGISEPLPVAE 120 Query: 125 TFTFTDEDGASLSDVARLDTMVTVVDAFNFLKDYSSQDSLDQRGQSMGEEDNRTVVDLLI 184 TFTFTDEDGASLSDVARLDTMVTVVDAFNFLKDYSSQDSLDQRGQSMGEEDNRTVVDLLI Sbjct: 121 TFTFTDEDGASLSDVARLDTMVTVVDAFNFLKDYSSQDSLDQRGQSMGEEDNRTVVDLLI 180 Query: 185 EQIEFCDVIVLNKLDLVAADDLERLHAILHKLNPRARIVRA 225 EQIEFCDVIVLNKLDLVAADDLERLHAILHKLNPRARIVRA Sbjct: 181 EQIEFCDVIVLNKLDLVAADDLERLHAILHKLNPRARIVRA 221 >gi|300310263|ref|YP_003774355.1| cobalamin synthesis P47K protein [Herbaspirillum seropedicae SmR1] Length = 413 Score = 233 bits (595), Expect = 1e-62, Method: Compositional matrix adjust. Identities = 123/212 (58%), Positives = 155/212 (73%), Gaps = 5/212 (2%) Query: 12 AADRRLPVTVLSGFLGAGKTTLLNHILHNREGRRVAVIVNDMSEVNIDAALVRDGGADLS 71 A +R+PVTVLSGFLG+GKTTLLNHIL NR G +VAVIVNDMSEVN+D VR +L Sbjct: 12 AQAQRIPVTVLSGFLGSGKTTLLNHILRNRAGMKVAVIVNDMSEVNMDGDDVRRN-TELY 70 Query: 72 RTEEKLVEMSNGCICCTLREDLLMEVRKLARERRFDQLVIESTGISEPLPVAETFTFTDE 131 R ++LVEMSNGCICCTLR DLL +V LAR RFD L+IESTGISEP+PVAETF F D Sbjct: 71 RGNDELVEMSNGCICCTLRADLLEQVSALARSGRFDYLLIESTGISEPIPVAETFAFLDS 130 Query: 132 DGASLSDVARLDTMVTVVDAFNFLKDYSSQDSLDQRGQSMGEEDNRTVVDLLIEQIEFCD 191 DG SLS++ARLDT+VTVV+ F + + ++ Q + R + DLLIEQ+E+ + Sbjct: 131 DGFSLSELARLDTLVTVVNGETFEQQLADHVAIAQADGA----STRPLSDLLIEQVEYAN 186 Query: 192 VIVLNKLDLVAADDLERLHAILHKLNPRARIV 223 VI+++++DL+ A L A+L KLNP ARI+ Sbjct: 187 VILVSRVDLIDASAFASLKAVLQKLNPTARIL 218 >gi|300314043|ref|YP_003778135.1| cobalamin synthesis protein/P47K family protein [Herbaspirillum seropedicae SmR1] Length = 388 Score = 122 bits (306), Expect = 4e-29, Method: Compositional matrix adjust. Identities = 78/216 (36%), Positives = 117/216 (54%), Gaps = 35/216 (16%) Query: 12 AADRRLPVTVLSGFLGAGKTTLLNHILHNREGRRVAVIVNDMSEVNIDAALVRDGGADLS 71 A D +PV++L+GFLG+GKTTLLNH++ E + VI+N+ E+ +D LV Sbjct: 7 AGDAPIPVSLLTGFLGSGKTTLLNHLITQPEMKDTLVIINEFGEIGLDHLLVAHS----- 61 Query: 72 RTEEKLVEMSNGCICCTLREDLLMEVRKLAR------ERRFDQLVIESTGISEPLPVAET 125 EE +VEMS+GC+CCT+R DL ++ + R+F+++VIE+TG++ P P+ T Sbjct: 62 -QEETIVEMSSGCLCCTIRGDLKKTLKDITWRFAEGGRRKFNRVVIETTGLASPAPILHT 120 Query: 126 FTFTDEDGASLSDVARLDTMVTVVDAFNFLKDYSSQDSLDQRGQSMGEEDNRTVVDLLIE 185 TD A +++ RLD ++ VDA N +LD ++ ++ Sbjct: 121 L-MTD---AFIAERYRLDGVIVTVDAVN------GMSTLDHHEEA-------------VQ 157 Query: 186 QIEFCDVIVLNKLDLVAADDLERLHAILHKLNPRAR 221 Q D ++L K DLV A L L A L LNP AR Sbjct: 158 QAAVADRLLLTKTDLVDAATLAALQARLRTLNPGAR 193 >gi|300311960|ref|YP_003776052.1| G3E family GTPase [Herbaspirillum seropedicae SmR1] Length = 377 Score = 84.3 bits (207), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 66/223 (29%), Positives = 114/223 (51%), Gaps = 20/223 (8%) Query: 3 IAMTSSQRQAADRRLPVTVLSGFLGAGKTTLLNHILHNREGRRVAVIVNDMSEVNIDAAL 62 +A++ + A +PVTV++GFLGAGKTTLL +L R+ RR+A+++N+ EV ID L Sbjct: 3 VALSLPELAPALPPIPVTVVTGFLGAGKTTLLRGLLQRRQSRRLALLINEFGEVAIDGDL 62 Query: 63 VRDGGADLSRTEEKLVEMSNGCICCTLREDLL-MEVRKLARERRFDQLVIESTGISEPLP 121 +R G ++ + ++ + ++G I + + AR + D ++IE++G++ P Sbjct: 63 LRAEGD--AQGQVQIQDFAHGLIAYGDDAHFVPAMLALAARRAQIDHVLIETSGLALPSA 120 Query: 122 VAETFTFTDEDGASLSDVARLDTMVTVVDAFNFLKD-YSSQDSLDQRGQSMGEEDNRTVV 180 V E GA ++ LD + VVD L+D ++S D++ Sbjct: 121 VMEVL-----QGAEMARHFVLDATLAVVDTPLLLEDAFASADAV-----------GAATA 164 Query: 181 DLLIEQIEFCDVIVLNKLDLVAADDLERLHAILHKLNPRARIV 223 L +Q+E DV+VLNK+D + L + + L P R + Sbjct: 165 QLFRQQLECADVVVLNKIDGMEEAPLLQAEQAVRALAPNVRFL 207 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.136 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 779,130 Number of extensions: 29192 Number of successful extensions: 162 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 156 Number of HSP's successfully gapped: 4 Length of query: 287 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 203 Effective length of database: 1,222,130 Effective search space: 248092390 Effective search space used: 248092390 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)