BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0037.p (260 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311864|ref|YP_003775956.1| enoyl-CoA hydratase [Herbaspiri... 528 e-151 gi|300313175|ref|YP_003777267.1| enoyl-CoA hydratase [Herbaspiri... 131 5e-32 gi|300313422|ref|YP_003777514.1| enoyl-CoA hydratase/isomerase [... 126 2e-30 gi|300313417|ref|YP_003777509.1| enoyl-CoA hydratase II protein ... 124 5e-30 gi|300312380|ref|YP_003776472.1| enoyl-CoA hydratase/carnithine ... 104 8e-24 >gi|300311864|ref|YP_003775956.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 260 Score = 528 bits (1360), Expect = e-151, Method: Compositional matrix adjust. Identities = 259/260 (99%), Positives = 260/260 (100%) Query: 1 MTTYTNLKLEKTGHTAVLTLSNPPANTWTRAALADLTRLVKELNADRDIYTLVVTGEGEK 60 MTTYTNLKLEKTGHTAVLTLSNPPANTWTRAALADLTRLVKELNADRDIYTLVVTGEGEK Sbjct: 1 MTTYTNLKLEKTGHTAVLTLSNPPANTWTRAALADLTRLVKELNADRDIYTLVVTGEGEK 60 Query: 61 FFSAGADLKLFADGDKAMAREMARRFGEAFETLSAFRGVSIAAINGYAMGGGLECALACD 120 FFSAGADLKLFADGDKAMAREMARRFGEAFETLSAFRGVSIAAINGYAMGGGLECALACD Sbjct: 61 FFSAGADLKLFADGDKAMAREMARRFGEAFETLSAFRGVSIAAINGYAMGGGLECALACD 120 Query: 121 IRIAEEQAQMALPEAAVGLLPCAGGTQNLPWLVGEGWAKRMILCGERVNAETALRIGLVE 180 IRIAEEQAQMALPEAAVGLLPCAGGTQNLPWLVGEGWAKRMILCGERVNAETALRIGLVE Sbjct: 121 IRIAEEQAQMALPEAAVGLLPCAGGTQNLPWLVGEGWAKRMILCGERVNAETALRIGLVE 180 Query: 181 EVVPRGQARERALALAQQAEKQSPSSVAASKQLIQGARHNPLGTILQTERELFVDLFDTQ 240 EVVPRGQARERALALAQQAEKQSPSSVAASKQLIQGARHNPLGTILQTERELFVDLFDTQ Sbjct: 181 EVVPRGQARERALALAQQAEKQSPSSVAASKQLIQGARHNPLGTILQTERELFVDLFDTQ 240 Query: 241 DQQEGVQAFLEKRSPQWKNQ 260 DQ+EGVQAFLEKRSPQWKNQ Sbjct: 241 DQREGVQAFLEKRSPQWKNQ 260 >gi|300313175|ref|YP_003777267.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 258 Score = 131 bits (330), Expect = 5e-32, Method: Compositional matrix adjust. Identities = 90/258 (34%), Positives = 133/258 (51%), Gaps = 3/258 (1%) Query: 3 TYTNLKLEKTGHTAVLTLSNPPA-NTWTRAALADLTRLVKELNADRDIYTLVVTGEGEKF 61 +Y N+++E ++ L P A N + + +L + +A +I +++TG EK Sbjct: 2 SYENIQVETHEKVGLIRLHRPKALNALSDGLMTELGEALLAFDAQEEIGCIIITGS-EKA 60 Query: 62 FSAGADLKLFADGDKAMAREMARRFGEAFETLSAFRGVSIAAINGYAMGGGLECALACDI 121 F+AGAD+ A G M +ETL R IAA+ GYA+GGG E A+ CD Sbjct: 61 FAAGADISAMA-GYDYMDVFKGEFITRNWETLRRIRKPVIAAVAGYALGGGCELAMMCDF 119 Query: 122 RIAEEQAQMALPEAAVGLLPCAGGTQNLPWLVGEGWAKRMILCGERVNAETALRIGLVEE 181 IA + A+ PE +G++P AGGTQ LP V + A + L G ++AE A R GLV Sbjct: 120 IIAADNAKFGQPEIKLGIVPGAGGTQRLPRAVSKAKAMDLALTGRMMDAEEAERAGLVSR 179 Query: 182 VVPRGQARERALALAQQAEKQSPSSVAASKQLIQGARHNPLGTILQTERELFVDLFDTQD 241 +V + E A+A A + K + A L ++ ER LF F T+D Sbjct: 180 IVAADKLLEEAMAAAIIIAEMPRQVAMMVKDSVNRAYETTLSEGMKYERALFYSCFATED 239 Query: 242 QQEGVQAFLEKRSPQWKN 259 Q+EG++AFLEKR P +K+ Sbjct: 240 QKEGMKAFLEKRQPVFKH 257 >gi|300313422|ref|YP_003777514.1| enoyl-CoA hydratase/isomerase [Herbaspirillum seropedicae SmR1] Length = 262 Score = 126 bits (317), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 92/261 (35%), Positives = 139/261 (53%), Gaps = 5/261 (1%) Query: 2 TTYTNLKLEKTG-HTAVLTLSNPPA-NTWTRAALADLTRLVKELNADRDIYTLVVTGEGE 59 Y +L + G H LTL+ P N +L ++ + D + +V++G E Sbjct: 5 VAYEDLLAARHGEHVLHLTLNRPALRNALRNQSLREIVAALARAEEDDSVRVVVISGN-E 63 Query: 60 KFFSAGADLKLFADGDKAMAREMARRFGEAFETLSAFRGVSIAAINGYAMGGGLECALAC 119 K F+AGADL D A+A ++ R + + ++ F +AA+NGYA+G G E + Sbjct: 64 KAFAAGADLNEMIHKD-AIATQLDVR-AQYWRAIARFPKPILAAVNGYALGAGCELLMHA 121 Query: 120 DIRIAEEQAQMALPEAAVGLLPCAGGTQNLPWLVGEGWAKRMILCGERVNAETALRIGLV 179 DI IA A++ PE VG LP AGGTQ L VG+ A +M+L GE ++A+ AL+ GLV Sbjct: 122 DIAIAARGAKIGQPEINVGTLPGAGGTQRLIRTVGKPLAMKMVLSGEFISADQALQAGLV 181 Query: 180 EEVVPRGQARERALALAQQAEKQSPSSVAASKQLIQGARHNPLGTILQTERELFVDLFDT 239 EVV ER LALA ++SP +V +K+ + + L L ER+ F + + Sbjct: 182 AEVVDDDATLERTLALAHSIAQKSPLAVRLAKEAMLQSFELGLEAGLLFERKSFSLMAAS 241 Query: 240 QDQQEGVQAFLEKRSPQWKNQ 260 D+QEG+ AF EKR+ + + Sbjct: 242 ADRQEGIAAFQEKRAAVFSGR 262 >gi|300313417|ref|YP_003777509.1| enoyl-CoA hydratase II protein [Herbaspirillum seropedicae SmR1] Length = 263 Score = 124 bits (312), Expect = 5e-30, Method: Compositional matrix adjust. Identities = 91/260 (35%), Positives = 132/260 (50%), Gaps = 6/260 (2%) Query: 6 NLKLEKTGHTAVLTLSNPPA-NTWTRAALADLTRLVKELNADRDIYTLVVTGEGEKFFSA 64 ++ LE AV+TL+ P N++T A +L + L AD + L++TG G + F A Sbjct: 5 SILLENRDGIAVITLNRPDKLNSFTVAMHLELRDAIATLQADPSVRVLLLTGAG-RGFCA 63 Query: 65 GADL--KLFADGDKA--MAREMARRFGEAFETLSAFRGVSIAAINGYAMGGGLECALACD 120 G DL + GD + + + +G ++L A I A+NG A G G LACD Sbjct: 64 GQDLGDRAVKPGDDGVDLGESIDKYYGPLVKSLRALPFPVICAVNGVAAGAGANLPLACD 123 Query: 121 IRIAEEQAQMALPEAAVGLLPCAGGTQNLPWLVGEGWAKRMILCGERVNAETALRIGLVE 180 I +A A +GL+P GGT LP LVG A M L GE+++AE A R GL+ Sbjct: 124 IVLAARSASFVEVFCKLGLIPDTGGTYFLPRLVGTARAMGMALLGEKISAEQAERWGLIW 183 Query: 181 EVVPRGQARERALALAQQAEKQSPSSVAASKQLIQGARHNPLGTILQTERELFVDLFDTQ 240 + V Q E A A+A + +AA+K + + + L L ER+ L ++ Sbjct: 184 KCVDDDQLMEEAHAMAAHFARAPTKGLAATKATLYASPAHTLPEQLDLERDTMRALGRSR 243 Query: 241 DQQEGVQAFLEKRSPQWKNQ 260 D +EGV AFLEKR+PQ+ Q Sbjct: 244 DYREGVTAFLEKRAPQFTGQ 263 >gi|300312380|ref|YP_003776472.1| enoyl-CoA hydratase/carnithine racemase [Herbaspirillum seropedicae SmR1] Length = 269 Score = 104 bits (259), Expect = 8e-24, Method: Compositional matrix adjust. Identities = 85/270 (31%), Positives = 129/270 (47%), Gaps = 18/270 (6%) Query: 1 MTTYTNLKLEKTGHTAVLTLSNPPA-NTWTRAALADLTRLVKELNADRDIYTLVVTGEGE 59 M++ +L + G A +TL+ P N + +A L L+ + D L++ G Sbjct: 1 MSSAAHLDITPRG-LATITLNRPEIHNAFDDQLIAHLIDLLTQARDDTRTCLLLLASTG- 58 Query: 60 KFFSAGADL---KLFADGDKAMAREMARRFGEAFETLSAFRGVSIAAINGYAMGGGLECA 116 K FSAGADL + AD + AR+ ETL++F ++A + G MGGG+ Sbjct: 59 KSFSAGADLAWMRRMADASREDNLRDARKLALLMETLNSFPTPTLAKVQGAVMGGGVGLV 118 Query: 117 LACDIRIAEEQAQMALPEAAVGLLPCAGGTQNLPWLV---GEGWAKRMILCGERVNAETA 173 CDI +A + A AL E +GL P A P+++ G A+R + ER A A Sbjct: 119 SCCDIVVASDAAFFALSEVKLGLAPAAIS----PYVIAKMGVSQARRYFVSAERFPAARA 174 Query: 174 LRIGLVEEVVPRGQARERALALAQQAEKQSPSSVAASKQLIQGARHNPLGTILQTERE-- 231 +GLV EV P + R L P ++ A+KQL+Q A NPL + Sbjct: 175 AAMGLVHEVAPPDELDARTAQLIDTLLANGPQAMRAAKQLVQVA--NPLQVTPELSEYTA 232 Query: 232 -LFVDLFDTQDQQEGVQAFLEKRSPQWKNQ 260 + DL + + +EG++AFL+K +P W Q Sbjct: 233 GVIADLRASAEGREGLRAFLDKDAPAWTRQ 262 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.132 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 774,045 Number of extensions: 31251 Number of successful extensions: 127 Number of sequences better than 1.0e-08: 10 Number of HSP's gapped: 117 Number of HSP's successfully gapped: 10 Length of query: 260 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 177 Effective length of database: 1,226,865 Effective search space: 217155105 Effective search space used: 217155105 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)