BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0036.p (386 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311865|ref|YP_003775957.1| acyl-CoA dehydrogenase [Herbasp... 797 0.0 gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbasp... 287 8e-79 gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [H... 216 4e-57 gi|300312109|ref|YP_003776201.1| acyl-CoA dehydrogenase [Herbasp... 145 7e-36 gi|300313303|ref|YP_003777395.1| acyl-CoA dehydrogenase [Herbasp... 135 6e-33 >gi|300311865|ref|YP_003775957.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 386 Score = 797 bits (2059), Expect = 0.0, Method: Compositional matrix adjust. Identities = 385/386 (99%), Positives = 385/386 (99%) Query: 1 MDFELNQDQLAFQQSARDFAAGEMAPFAAKWDEEAHFPLDVIAKAGELGFCGLYTPEADG 60 MDFELNQDQLAFQQSARDFAAGEMAPFAAKWDEEAHFPLDVIAKAGELGFCGLYTPEADG Sbjct: 1 MDFELNQDQLAFQQSARDFAAGEMAPFAAKWDEEAHFPLDVIAKAGELGFCGLYTPEADG 60 Query: 61 GLGLSRLDATVVFEELARACTSTAAYLTIHNMVSWMIASWGQPSLKAQWCEQLATGRKIG 120 GLGLSRLDATVVFEELARACTSTAAYLTIHNMVSWMIASWGQPSLKAQWCEQLA GRKIG Sbjct: 61 GLGLSRLDATVVFEELARACTSTAAYLTIHNMVSWMIASWGQPSLKAQWCEQLAAGRKIG 120 Query: 121 SYCLTEPGSGSDAASLKTTARLEDGHYVLNGSKAFISGAGSTDVLVVMARTGGEGARGVS 180 SYCLTEPGSGSDAASLKTTARLEDGHYVLNGSKAFISGAGSTDVLVVMARTGGEGARGVS Sbjct: 121 SYCLTEPGSGSDAASLKTTARLEDGHYVLNGSKAFISGAGSTDVLVVMARTGGEGARGVS 180 Query: 181 AIVVPGNAPGVSYGKKESKMGWNSQPTRTISFDNVKVPADHLLGQEGQGFIFAMKGLDGG 240 AIVVPGNAPGVSYGKKESKMGWNSQPTRTISFDNVKVPADHLLGQEGQGFIFAMKGLDGG Sbjct: 181 AIVVPGNAPGVSYGKKESKMGWNSQPTRTISFDNVKVPADHLLGQEGQGFIFAMKGLDGG 240 Query: 241 RINIATCSVGAAQAALDAAHAYMKERKQFGRPIADFQALQFKLADMQTELVAARQMVRLA 300 RINIATCSVGAAQAALDAAHAYMKERKQFGRPIADFQALQFKLADMQTELVAARQMVRLA Sbjct: 241 RINIATCSVGAAQAALDAAHAYMKERKQFGRPIADFQALQFKLADMQTELVAARQMVRLA 300 Query: 301 ATKLDAGSPEATTYCAMAKRLATDLGFRVCNEALQLHGGYGYIREYPLERYFRDVRVHQI 360 ATKLDAGSPEATTYCAMAKRLATDLGFRVCNEALQLHGGYGYIREYPLERYFRDVRVHQI Sbjct: 301 ATKLDAGSPEATTYCAMAKRLATDLGFRVCNEALQLHGGYGYIREYPLERYFRDVRVHQI 360 Query: 361 LEGTNEIMRVIISRKLLDRDNLDDIR 386 LEGTNEIMRVIISRKLLDRDNLDDIR Sbjct: 361 LEGTNEIMRVIISRKLLDRDNLDDIR 386 >gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 382 Score = 287 bits (735), Expect = 8e-79, Method: Compositional matrix adjust. Identities = 150/374 (40%), Positives = 225/374 (60%), Gaps = 3/374 (0%) Query: 5 LNQDQLAFQQSARDFAAGEMAPFAAKWDEEAHFPLDVIAKAGELGFCGLYTPEADGGLGL 64 L+ + + + R FA + PFAA WD FP + + + ELG G+ PE GG G+ Sbjct: 3 LSPEHEMIRDAMRHFAQERLLPFAADWDRNHTFPAEALKELAELGAMGMCVPEQWGGAGM 62 Query: 65 SRLDATVVFEELARACTSTAAYLTIHNMVSWMIA-SWGQPSLKAQWCEQLATGRKIGSYC 123 + + EE+A +T+ +++ N ++ I +G P+ K QW + LA G +G +C Sbjct: 63 DYMSLVLALEEIAAGDGATSTIVSVQNSLACGITEKYGSPTQKEQWLKPLARGEMLGCFC 122 Query: 124 LTEPGSGSDAASLKTTARLEDGHYVLNGSKAFISGAGSTDVLVVMARTG-GEGARGVSAI 182 LTEP +GSDAA+++T A + ++LNG+K FI+ V +V A T G +G+S Sbjct: 123 LTEPHTGSDAAAIRTRAERDGDDFILNGTKQFITSGKHAGVAIVFAVTDRSAGKKGISCF 182 Query: 183 VVPGNAPGVSYGKKESKMGWNSQPTRTISFDNVKVPADHLLGQEGQGFIFAMKGLDGGRI 242 +VP + PG G+ E KMG ++ T I DN +VPA LLG+EG+G+ A+ L+ GRI Sbjct: 183 LVPCDTPGFVVGRSEEKMGQHASDTVQIMLDNCRVPATALLGKEGEGYRIALSNLEAGRI 242 Query: 243 NIATCSVGAAQAALDAAHAYMKERKQFGRPIADFQALQFKLADMQTELVAARQMVRLAAT 302 IA SVG A+AA +AA +Y ++R+ FG PI + QA+ F+LADM T L AAR MV AA Sbjct: 243 GIAAQSVGMARAAFEAAVSYARQRESFGVPIIEHQAVNFRLADMNTLLDAARLMVWRAAQ 302 Query: 303 KLDAGSPEATTYCAMAKRLATDLGFRVCNEALQLHGGYGYIREYPLERYFRDVRVHQILE 362 D G P +MAK A++ ++ ++A+Q+HGG GY ++P+ER +RDVR+ QI E Sbjct: 303 LKDQGRP-CLKEASMAKMFASEAAEKIASDAIQIHGGVGYTSDFPVERIYRDVRICQIYE 361 Query: 363 GTNEIMRVIISRKL 376 G N+I R++I R + Sbjct: 362 GANDIQRLVIGRAI 375 >gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 394 Score = 216 bits (549), Expect = 4e-57, Method: Compositional matrix adjust. Identities = 136/385 (35%), Positives = 206/385 (53%), Gaps = 9/385 (2%) Query: 1 MDFELNQDQLAFQQSARDFAAGEMAPFAAKWDEEAHFPLDVIAKAGELGFCGLYTPEADG 60 + F+ +D A +++ FA E+AP AA+ D FP+D+ K G+LG G+ E G Sbjct: 7 LSFDHGEDIAALREAVAAFAHSEIAPRAAEIDRSDQFPMDLWKKLGDLGVLGITVSEEYG 66 Query: 61 GLGLSRLDATVVFEELARACTSTAAYLTIH-NMVSWMIASWGQPSLKAQWCEQLATGRKI 119 G GL L + EE++RA S H N+ I G K ++ +L +G I Sbjct: 67 GAGLGYLAHIIAMEEISRASASVGLSYGAHSNLCVNQIKRNGNEEQKRKYLPRLISGDFI 126 Query: 120 GSYCLTEPGSGSDAASLKTTARLEDGHYVLNGSKAFISGAGSTDVLVVMARTGGE-GARG 178 G+ ++EP +GSD S+K A + YVLNGSK +I+ DVLVV A+T E GARG Sbjct: 127 GALAMSEPNAGSDVVSMKLRADKKGDRYVLNGSKMWITNGPDADVLVVYAKTDLEAGARG 186 Query: 179 VSAIVVPGNAPGVSYGKKESKMGWNSQPTRTISFDNVKVPADHLLGQEGQGFIFAMKGLD 238 ++A +V G S +K K+G T + F + +VP +++LG G+G M GLD Sbjct: 187 MTAFLVEKGYKGFSVAQKLDKLGMRGSHTGELVFQDCEVPEENVLGGVGRGVNVLMSGLD 246 Query: 239 GGRINIATCSVGAAQAALDAAHAYMKERKQFGRPIADFQALQFKLADMQTELVAARQMVR 298 R ++ +G QA +D Y+ +RKQFG+ I +FQ +Q KLADM + ++A + V Sbjct: 247 FERSVLSGGPLGIMQACMDVVVPYVHDRKQFGQAIGEFQLMQGKLADMYSTMMACKAYVY 306 Query: 299 LAATKLDAGSPEATTYCAMAKRLATDLGFR------VCNEALQLHGGYGYIREYPLERYF 352 D + A A+ K A + + + EA+Q GG GYI EYP+ R + Sbjct: 307 AVGQACDR-ADSADKVRALRKDAAGAILYSAEKATWMAGEAIQSLGGNGYINEYPVGRLW 365 Query: 353 RDVRVHQILEGTNEIMRVIISRKLL 377 RD ++++I GT+EI R++I R+L Sbjct: 366 RDAKLYEIGAGTSEIRRMLIGRELF 390 >gi|300312109|ref|YP_003776201.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 388 Score = 145 bits (365), Expect = 7e-36, Method: Compositional matrix adjust. Identities = 109/374 (29%), Positives = 188/374 (50%), Gaps = 12/374 (3%) Query: 12 FQQSARDFAAGEMAPFAAKWDEEAHFPLDVIAKAGELGFCGLYTPEADGGLGLSRLDATV 71 + + RD + K DE +P + + G+ P+ GG GL +A+V Sbjct: 13 LRDAVRDLCKTFPPEYWRKIDEARGYPEQFVDALTKAGWLAALIPQEYGGSGLGLTEASV 72 Query: 72 VFEELARAC-TSTAAYLTIHNMVSWMIASWGQPSLKAQWCEQLATGR-KIGSYCLTEPGS 129 + EE+ R S A + ++NM + + G P K ++ ++A+G ++ S +TEP + Sbjct: 73 IMEEINRTGGNSGACHGQMYNMGTLL--RHGSPEQKERYLPRIASGELRLQSMAVTEPTT 130 Query: 130 GSDAASLKTTARLEDGHYVLNGSKAFISGAGSTDVLVVMARTG-----GEGARGVSAIVV 184 G+D +KT A + YV+NG K +IS +D+++++ART + G+S +V Sbjct: 131 GTDTTRIKTVAVKKGDRYVINGQKVWISRVQHSDLMILLARTTPLEQVSRKSEGMSIFLV 190 Query: 185 PGNAPGVSYGKK-ESKMGWNSQPTRTISFDNVKVPADHLLGQEGQGFIFAMKGLDGGRIN 243 + +G + + + T + FDN+++PA++L+G+EG+GF + + GL+ R Sbjct: 191 DLKE-AIGHGMEVRPILNMVNHETNELFFDNLEIPAENLIGEEGRGFRYILDGLNAERTL 249 Query: 244 IATCSVGAAQAALDAAHAYMKERKQFGRPIADFQALQFKLADMQTELVAARQMVRLAATK 303 IA +G +D A Y +ER F RPI Q +QF LA+ E+ AA ++R A Sbjct: 250 IAAECIGDGYWFIDKATQYARERVVFDRPIGMNQGVQFPLAEAYIEVEAA-NLMRYQACA 308 Query: 304 LDAGSPEATTYCAMAKRLATDLGFRVCNEALQLHGGYGYIREYPLERYFRDVRVHQILEG 363 L MAK LA + N LQ HGG+G+ EY +ER FR+ R++Q+ Sbjct: 309 LFDAQQSCGAQANMAKYLAAKAAWEAANVCLQTHGGFGFACEYDVERKFRETRLYQVAPI 368 Query: 364 TNEIMRVIISRKLL 377 + ++ ++ +L Sbjct: 369 STNLIFSYVAEHVL 382 >gi|300313303|ref|YP_003777395.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 591 Score = 135 bits (340), Expect = 6e-33, Method: Compositional matrix adjust. Identities = 114/372 (30%), Positives = 180/372 (48%), Gaps = 39/372 (10%) Query: 49 GFCGLYTPEADGGLGLSRLDATVVFEELARACTSTAAYLTIHNMVSWMIASWGQPSLKAQ 108 G+ L GG GL + ++E L+ A Y + + +A+ G +L+A+ Sbjct: 91 GWPALACAPEHGGQGLPHVLDCALYEMLSAANHGWTMYPGLLHGAYACLAAHGSAALQAR 150 Query: 109 WCEQLATGRKIGSYCLTEPGSGSDAASLKTTA-RLEDGHYVLNGSKAFISGAGSTD---- 163 + +++A+G + + CLTE +GSD L+T A +DG Y L+G K FISG G D Sbjct: 151 YLDKIASGEWLATMCLTEAQAGSDLGQLRTRAVPQDDGSYRLSGDKIFISG-GEHDLTPN 209 Query: 164 -VLVVMART--GGEGARGVSAIVVP-----GNAPGVSYGKKESKMGWNSQPTRTISFDNV 215 V +V+AR G++G+S + P G+ GV E KMG + T ++ F+ Sbjct: 210 IVHLVLARLPDAPAGSKGLSLFLAPRVLDDGSRNGVHCTGIEHKMGIHGSATCSLHFEQA 269 Query: 216 KVPADHLLGQEGQGFIFAMKGLDGGRINIATCSVGAAQAALDAAHAYMKERKQFGRP--- 272 + L+G+ G+G ++ R+++ +G A+AA A AY +ER+Q P Sbjct: 270 QA---WLVGEAGRGLAAMFVMMNAARLHVGAQGLGLAEAAYQRASAYARERRQSRAPGSS 326 Query: 273 ----IADFQALQFKLADMQTELVAARQMVRLAATKLDAGS--PEATTYCAMAKRLA---- 322 I A+Q L + + + AR + A LD PEA +RL Sbjct: 327 GNDLIVHHPAVQKLLMEQRCRIEGARLLTCWAGLLLDLAEHDPEAALRSRHHERLEFITP 386 Query: 323 ------TDLGFRVCNEALQLHGGYGYIREYPLERYFRDVRVHQILEGTNEIMRV-IISRK 375 TD GF+ + ALQ+ GG+GY+ E +E++ RD R+ I EGTNEI + + RK Sbjct: 387 VIKAFLTDQGFQCASRALQVFGGHGYVVETGIEQFMRDARITMIYEGTNEIQAIDFLMRK 446 Query: 376 LLDRD--NLDDI 385 +L + LDD Sbjct: 447 VLGDEGRRLDDF 458 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.134 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,225,164 Number of extensions: 52503 Number of successful extensions: 184 Number of sequences better than 1.0e-08: 13 Number of HSP's gapped: 157 Number of HSP's successfully gapped: 13 Length of query: 386 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 299 Effective length of database: 1,207,925 Effective search space: 361169575 Effective search space used: 361169575 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)