BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0028.p (235 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311872|ref|YP_003775964.1| glutathione S-transferase famil... 481 e-137 gi|300312317|ref|YP_003776409.1| glutathione S-transferase [Herb... 228 4e-61 gi|300310406|ref|YP_003774498.1| glutathione S-transferase [Herb... 63 2e-11 gi|300311017|ref|YP_003775109.1| glutathione S-transferase famil... 62 5e-11 >gi|300311872|ref|YP_003775964.1| glutathione S-transferase family protein [Herbaspirillum seropedicae SmR1] Length = 235 Score = 481 bits (1239), Expect = e-137, Method: Compositional matrix adjust. Identities = 235/235 (100%), Positives = 235/235 (100%) Query: 1 MPHALSSFAIDQKWPAQHPDRIQLYSLPTPNGVKVSIMLEETGLPYEAHLVSFETNDQMS 60 MPHALSSFAIDQKWPAQHPDRIQLYSLPTPNGVKVSIMLEETGLPYEAHLVSFETNDQMS Sbjct: 1 MPHALSSFAIDQKWPAQHPDRIQLYSLPTPNGVKVSIMLEETGLPYEAHLVSFETNDQMS 60 Query: 61 PEFLSLNPNNKIPAIIDPNGPGGQPLPLFESGAILLYLAEKTGQFLPADAAARYQVIQWV 120 PEFLSLNPNNKIPAIIDPNGPGGQPLPLFESGAILLYLAEKTGQFLPADAAARYQVIQWV Sbjct: 61 PEFLSLNPNNKIPAIIDPNGPGGQPLPLFESGAILLYLAEKTGQFLPADAAARYQVIQWV 120 Query: 121 MFQMGGVGPMFGQLGFFHRFAGKEYEDKRPRDRYVNESKRLLGVMDKRLAQSQWLGGDAY 180 MFQMGGVGPMFGQLGFFHRFAGKEYEDKRPRDRYVNESKRLLGVMDKRLAQSQWLGGDAY Sbjct: 121 MFQMGGVGPMFGQLGFFHRFAGKEYEDKRPRDRYVNESKRLLGVMDKRLAQSQWLGGDAY 180 Query: 181 SIADIATFPWIRNLIGFYEAADLVGFQDFPHVARALEAFVARPAVVRGLNIPARG 235 SIADIATFPWIRNLIGFYEAADLVGFQDFPHVARALEAFVARPAVVRGLNIPARG Sbjct: 181 SIADIATFPWIRNLIGFYEAADLVGFQDFPHVARALEAFVARPAVVRGLNIPARG 235 >gi|300312317|ref|YP_003776409.1| glutathione S-transferase [Herbaspirillum seropedicae SmR1] Length = 230 Score = 228 bits (581), Expect = 4e-61, Method: Compositional matrix adjust. Identities = 113/212 (53%), Positives = 144/212 (67%), Gaps = 7/212 (3%) Query: 22 IQLYSLPTPNGVKVSIMLEETGLPYEAHLVSFETNDQMSPEFLSLNPNNKIPAIIDPNGP 81 I +YS TPNG KV IMLEE GLPY AH + DQ +PEFL ++PNNKIPAI+DP+GP Sbjct: 2 IDVYSWATPNGHKVHIMLEECGLPYTAHAIDIGAGDQFTPEFLKISPNNKIPAIVDPDGP 61 Query: 82 GGQPLPLFESGAILLYLAEKTGQFLPADAAARYQVIQWVMFQMGGVGPMFGQLGFFHRFA 141 G+P+ LFESGAIL+YLA KTG+FL +Y+V+QWVMFQMGG+GPM GQ F +A Sbjct: 62 DGEPISLFESGAILIYLAGKTGRFLGETTREKYEVLQWVMFQMGGLGPMLGQAHHFRLYA 121 Query: 142 GKEYEDKRPRDRYVNESKRLLGVMDKRLAQSQWLGGDAYSIADIATFPWIRNLIGFYEAA 201 ++ E +RY NE++RL VMDK+L Q +L G+ Y+IADIA FPW R+ Sbjct: 122 PEQIE--YAVNRYTNEARRLYQVMDKQLGQHAYLAGEHYTIADIACFPWTRS-----HKN 174 Query: 202 DLVGFQDFPHVARALEAFVARPAVVRGLNIPA 233 + DFP+V R +A ARPAV RG+ + A Sbjct: 175 QGIDLDDFPNVKRWYDAISARPAVQRGVTVLA 206 >gi|300310406|ref|YP_003774498.1| glutathione S-transferase [Herbaspirillum seropedicae SmR1] Length = 206 Score = 62.8 bits (151), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 56/185 (30%), Positives = 83/185 (44%), Gaps = 22/185 (11%) Query: 43 GLPYEAHLVSFETNDQMSPEFLSLNPNNKIPAIIDPNGPGGQPLPLFESGAILLYLAEKT 102 GLP E V P FL+LNP ++P + D + + +S AIL+YLA+K Sbjct: 23 GLPAELVEVDLAAGAHKKPAFLALNPFGQVPVLEDGE------VVIADSNAILVYLAKKA 76 Query: 103 GQ--FLPADAAARYQVIQWVMFQMG--GVGPMFGQLG--FFHRFAGKEYEDKRPRDRYVN 156 G+ +LP DA V +W+ G GP +L F F +E + Sbjct: 77 GRSDWLPEDAQGAAAVQRWLSVAAGELAYGPAAARLVTVFGAAFNAEEV---------IQ 127 Query: 157 ESKRLLGVMDKRLAQSQWLGGDAYSIADIATFPWI-RNLIGFYEAADLVGFQDFPHVARA 215 + LLG +++ LA+ WL D +IAD+A + ++ R G + G Q F A Sbjct: 128 RAHLLLGRLEQHLAKRDWLAADHPTIADVAVYSYLARAPEGNVDLRGYPGVQGFLQRIEA 187 Query: 216 LEAFV 220 L FV Sbjct: 188 LPGFV 192 >gi|300311017|ref|YP_003775109.1| glutathione S-transferase family protein [Herbaspirillum seropedicae SmR1] Length = 223 Score = 62.0 bits (149), Expect = 5e-11, Method: Compositional matrix adjust. Identities = 67/227 (29%), Positives = 91/227 (40%), Gaps = 32/227 (14%) Query: 22 IQLYSLPTPNGVKVSIMLEETGLPYEAHLVSFETNDQMSP-EFLSLNPNNKIPAIIDPNG 80 I ++ L +V +LEE GL YE + ++P E L ++P K P I D Sbjct: 2 ITVHHLNNSRSQRVLWLLEELGLDYEIKFYQRDPKTMLAPPELLKVHPLGKSPVITDGE- 60 Query: 81 PGGQPLPLFESGAILLYLAEKTGQ---FLPADAA-ARYQVIQWVMFQMGGVGPMFGQLGF 136 L + ESG I+ YL E+ G LPA R + W+ F G P Sbjct: 61 -----LTVAESGNIIEYLLERYGAHSGLLPASGTPERLRYRYWLHFAEGSAMPWLVMQLI 115 Query: 137 FHRFAGKEYEDKRPRDRYVNE----------SKRLLGVMDKRLAQSQWLGGDAYSIADI- 185 F + RP R + E +R L M+ L Q W G +S ADI Sbjct: 116 FAKMPKAVPTLIRPIARMLAEGMRSRVVAPQQERQLRFMEDELGQQGWFAGAQFSGADIQ 175 Query: 186 ATFPWIRNLIGFYEAADLVGFQD--FPHVARALEAFVARPAVVRGLN 230 +FP EA G D +P++ + LEA ARPA R L Sbjct: 176 MSFP--------VEALRARGGLDGRYPNLLKYLEAIHARPAYQRALE 214 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.140 0.433 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 844,836 Number of extensions: 37627 Number of successful extensions: 103 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 98 Number of HSP's successfully gapped: 4 Length of query: 235 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 153 Effective length of database: 1,231,600 Effective search space: 188434800 Effective search space used: 188434800 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 129 (54.3 bits)