BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0024.p (431 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311875|ref|YP_003775967.1| aspartate/tyrosine/aromatic ami... 879 0.0 gi|300312878|ref|YP_003776970.1| aspartate/tyrosine/aromatic ami... 122 6e-29 gi|300311478|ref|YP_003775570.1| succinyldiaminopimelate aminotr... 121 1e-28 gi|300312316|ref|YP_003776408.1| pyridoxal phosphate dependent a... 114 2e-26 gi|300311409|ref|YP_003775501.1| aspartate aminotransferase [Her... 109 4e-25 >gi|300311875|ref|YP_003775967.1| aspartate/tyrosine/aromatic aminotransferase [Herbaspirillum seropedicae SmR1] Length = 431 Score = 879 bits (2271), Expect = 0.0, Method: Compositional matrix adjust. Identities = 428/431 (99%), Positives = 430/431 (99%) Query: 1 MTQSADSRDPSRSPDVSAASAASSASAASPVVPPDAFPVSFPRIERLPPYVFNITAELKM 60 MTQSADSRDPSRSPD SAAS+AS+ASAASPVVPPDAFPVSFPRIERLPPYVFNITAELKM Sbjct: 1 MTQSADSRDPSRSPDASAASSASAASAASPVVPPDAFPVSFPRIERLPPYVFNITAELKM 60 Query: 61 AARRRGEDIIDMSMGNPDGATPPHIVEKLVEVAQRPDTHGYSSSKGIPRLRRAIAHWYRS 120 AARRRGEDIIDMSMGNPDGATPPHIVEKLVEVAQRPDTHGYSSSKGIPRLRRAIAHWYRS Sbjct: 61 AARRRGEDIIDMSMGNPDGATPPHIVEKLVEVAQRPDTHGYSSSKGIPRLRRAIAHWYRS 120 Query: 121 RYEVDFDPDSEAIVTIGSKEGLAHLMLATLDKGDTVLVPNPSYPIHIYGAVIAGANIRSV 180 RYEVDFDPDSEAIVTIGSKEGLAHLMLATLDKGDTVLVPNPSYPIHIYGAVIAGANIRSV Sbjct: 121 RYEVDFDPDSEAIVTIGSKEGLAHLMLATLDKGDTVLVPNPSYPIHIYGAVIAGANIRSV 180 Query: 181 RMSPGVDFFDELERAVRESYPKPKMMILGFPSNPTAQCVELEFFERVVKLAREHQILVVH 240 RMSPGVDFFDELERAVRESYPKPKMMILGFPSNPTAQCVELEFFERVVKLAREHQILVVH Sbjct: 181 RMSPGVDFFDELERAVRESYPKPKMMILGFPSNPTAQCVELEFFERVVKLAREHQILVVH 240 Query: 241 DLAYADITFDGWKAPSIMQVPGAREVAVEFFTLSKSYNMAGWRIGFMVGNARLVAALARI 300 DLAYADITFDGWKAPSIMQVPGAREVAVEFFTLSKSYNMAGWRIGFMVGNARLVAALARI Sbjct: 241 DLAYADITFDGWKAPSIMQVPGAREVAVEFFTLSKSYNMAGWRIGFMVGNARLVAALARI 300 Query: 301 KSYHDYGSFTPVQVAAIAALEGDQSCVEEIRANYERRRNVLVKGLHEAGWMVDVPKASMY 360 KSYHDYGSFTPVQVAAIAALEGDQSCVEEIRANYERRRNVLVKGLHEAGWMVDVPKASMY Sbjct: 301 KSYHDYGSFTPVQVAAIAALEGDQSCVEEIRANYERRRNVLVKGLHEAGWMVDVPKASMY 360 Query: 361 IWARIPEPYRQFGSLEFARILLEQAKVCVSPGIGFGEYGDEYVRFALIENESRIRQAIRG 420 IWARIPEPYRQFGSLEFARILLEQAKVCVSPGIGFGEYGDEYVRFALIENESRIRQAIRG Sbjct: 361 IWARIPEPYRQFGSLEFARILLEQAKVCVSPGIGFGEYGDEYVRFALIENESRIRQAIRG 420 Query: 421 IKAMFKNAKGS 431 IKAMFKNAKGS Sbjct: 421 IKAMFKNAKGS 431 >gi|300312878|ref|YP_003776970.1| aspartate/tyrosine/aromatic aminotransferase [Herbaspirillum seropedicae SmR1] Length = 393 Score = 122 bits (306), Expect = 6e-29, Method: Compositional matrix adjust. Identities = 105/395 (26%), Positives = 189/395 (47%), Gaps = 21/395 (5%) Query: 43 RIERLPPYVFNITAELKMAARRRGEDIIDMSMGNPDGATPPHIVEKLVEVAQRPDTHG-- 100 R++ + P+ ++ ++G +I M +G PD PP +VE A R T G Sbjct: 9 RLQHIAPFHVMELSKKAAVLEQQGRHLIHMGIGEPDFTAPPAVVE----AATRAMTEGKM 64 Query: 101 -YSSSKGIPRLRRAIAHWYRSRYEVDFDPDSEAIVTIGSKEGLAHLMLATLDKGDTVLVP 159 Y+S+ G+P+LR AI+ YRS Y ++ P+ +VT G+ L A +++ VL+P Sbjct: 65 QYTSATGLPQLRAAISDHYRSVYGLEIAPE-RIVVTAGASAALLLACAALVERDSEVLMP 123 Query: 160 NPSYPIHIYGAVIAGANIRSVRMSPGVDF-FDELERAVRESY-PKPKMMILGFPSNPTAQ 217 +PSYP + + +A R+ ++ G + F + VRE + + ++L PSNPT Sbjct: 124 DPSYPCNRH--FVAAFEGRAKLIASGPEHRFQLSAQMVREHWGSATRGVLLASPSNPTGT 181 Query: 218 CVELEFFERVVKLAREHQILVVHDLAYADITFDGWKAPSIMQVPGAREVAVEFFTLSKSY 277 + + +V R+ + D Y ++++G AP G V + F SK + Sbjct: 182 SILPDELRAIVGEVRQRGGFTIVDEIYQGLSYEG--APFSALSLGDDVVVINSF--SKYF 237 Query: 278 NMAGWRIGFMVGNARLVAALARIKSYHDYGSFTPVQVAAIAALEGDQ-SCVEEIRANYER 336 NM GWR+G++V LV + ++ + + Q AA+A + EE +A ++R Sbjct: 238 NMTGWRLGWLVLPPALVPQVEKLAQNLLICASSIAQHAAVACFTPQTLALYEERKAEFKR 297 Query: 337 RRNVLVKGLHEAGWMVDV-PKASMYIWARIPEPYRQFGSLEFARILLEQAKVCVSPGIGF 395 RR+ +V L G+ V V P + Y++A L +L +A V + PG+ F Sbjct: 298 RRDYIVPALESLGFTVPVMPDGAFYVYADCSALADDADQLSLD--MLNEAGVVLVPGLDF 355 Query: 396 GEY-GDEYVRFALIENESRIRQAIRGIKAMFKNAK 429 G + Y+R + + +++A+ ++ F+ + Sbjct: 356 GPFTARRYIRLSYATSMENLQEAVARLRVFFEKRR 390 >gi|300311478|ref|YP_003775570.1| succinyldiaminopimelate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 420 Score = 121 bits (303), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 104/406 (25%), Positives = 183/406 (45%), Gaps = 24/406 (5%) Query: 42 PRIERLPPYVFNITAELKMAARRR-GEDIIDMSMGNPDGATPPHIVEKLVEVAQRPDTHG 100 P +++L PY F +L I + +G P TP I + L++ Sbjct: 20 PLLDQLQPYPFEKLKKLFAGITPDPAYRPISLGIGEPKHPTPAFIQQALID--NLAGLAS 77 Query: 101 YSSSKGIPRLRRAIAHWYRSRYEVDF-DPDSEAIVTIGSKEGLAHLMLATLDK---GDTV 156 Y ++ G LR AIA W + RY++ DP +E + GS+E L L +D G V Sbjct: 78 YPATLGSDALRGAIADWLQKRYDIPRPDPATEILPVNGSREALFSLTQTVVDPTRPGALV 137 Query: 157 LVPNPSYPIHIYGAVIAGANIRSVRMSPGVDFFDELERAVRESYPKPKMMILGFPSNPTA 216 + PNP Y I+ A +AGA V P +F + + ++ + + +++ + P NPT Sbjct: 138 MCPNPFYQIYEGSAYLAGAQPYFVNSDPKRNFAPDFSQVPQDVWERVQLLFVCSPGNPTG 197 Query: 217 QCVELEFFERVVKLAREHQILVVHDLAYADITFDGWKAPSIMQVPGAREVA------VEF 270 + LE ++ + L+ + ++ D Y++I F+ + P + + AR + + F Sbjct: 198 AVLTLEDWKELFALSDRYGFVIASDECYSEIYFN--EDPPLGGMQAARLLGRDYRRLISF 255 Query: 271 FTLSKSYNMAGWRIGFMVGNARLVAALARIKSYHDYGSFTPVQVAAIAALEGDQSCVEEI 330 +LSK N+ G R GF+ G+A ++ ++YH +Q A++ A + D++ V+ Sbjct: 256 SSLSKRSNVPGMRSGFVAGDAAILKKFLLYRTYHGAAMSPVIQAASVLAWQ-DETHVQGN 314 Query: 331 RANYERRRNVLVKGLHEAGWMVDVPKASMYIWARIPEPYRQFGSLEFARILLEQAKVCVS 390 A Y + + + L E V +P A+ Y+WA++ + EFA+ L + V V Sbjct: 315 IAKYVTKFSQVTPLLQEV-LEVALPDAAFYLWAKV-DKLVNISDTEFAQRLYAEYNVTVL 372 Query: 391 PGIGFGEY------GDEYVRFALIENESRIRQAIRGIKAMFKNAKG 430 PG G +R AL+ +A + I A + G Sbjct: 373 PGSYLAREAHGVNPGAGRIRMALVAEVEECLEAAQRIVAFTRKLAG 418 >gi|300312316|ref|YP_003776408.1| pyridoxal phosphate dependent aspartate/tyrosine/aromatic aminotransferase [Herbaspirillum seropedicae SmR1] Length = 385 Score = 114 bits (284), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 93/382 (24%), Positives = 165/382 (43%), Gaps = 14/382 (3%) Query: 44 IERLPPYVFNITAELKMAARRRGEDIIDMSMGNPDGATPPHIVEKLVEVAQRPDTHGYSS 103 + +LP I + A +G +++ G PD P +V + + Q + Y Sbjct: 7 VTKLPKVGTTIFTVMSALASEKGA--VNLGQGFPDFHCDPALVAAVTQAMQE-GLNQYPP 63 Query: 104 SKGIPRLRRAIAHWYRSRYEVDFDPDSEAIVTIGSKEGLAHLMLATLDKGDTVLVPNPSY 163 G+P LR AIA Y +DP SE VT G+ +G+ +L + GD V+V P Y Sbjct: 64 MAGVPVLREAIAEKVEKLYGHRYDPVSEITVTAGATQGILTSVLCAVHPGDEVIVIEPVY 123 Query: 164 PIHIYGAVIAGANIRSVRMSPGVDFF----DELERAVRESYPKPKMMILGFPSNPTAQCV 219 ++ +AG V+M G + D+++ AV PK +M+++ P NPT + Sbjct: 124 DCYVPAIELAGGVPVFVQMEVGAQGYSIPWDKVKAAVT---PKTRMIMVNTPHNPTGSVM 180 Query: 220 ELEFFERVVKLAREHQILVVHDLAYADITFDGWKAPSIMQVPGAREVAVEFFTLSKSYNM 279 + + R IL++ D Y + +DG S+ + P E + + K+Y++ Sbjct: 181 RAADVAALADIVRGTDILILSDEVYEHMVYDGQPHESLARHPELAERSFINSSFGKTYHV 240 Query: 280 AGWRIGFMVGNARLVAALARIKSYHDYGSFTPVQVAAIAALEGDQSCVEEIRANYERRRN 339 GW++G++ A L A ++ ++ + TPVQ +AA D + ++ A Y+ +R+ Sbjct: 241 TGWKVGYVAAPAALTAEFRKVHQFNVFTVNTPVQY-GLAAYMKDPAPYLDLPAFYQHKRD 299 Query: 340 VLVKGLHEAGWMVDVPKASMYIWARIPEPYRQFGSLEFARILLEQAKVCVSPGIGFGEYG 399 + GL + + +P Y EF + L + V P F Sbjct: 300 LFRTGLANTRFEL-LPSQGTYFQCVKYGAISALPEAEFCKWLTSEIGVAAIPVSAFYNTP 358 Query: 400 DE--YVRFALIENESRIRQAIR 419 E VRF + + ++ A+ Sbjct: 359 RESGIVRFCFAKKDETLQLALE 380 >gi|300311409|ref|YP_003775501.1| aspartate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 409 Score = 109 bits (273), Expect = 4e-25, Method: Compositional matrix adjust. Identities = 96/390 (24%), Positives = 183/390 (46%), Gaps = 21/390 (5%) Query: 45 ERLPPYVFNITAELKMAARR---RGEDIIDMSMGNPDG---ATPPHIVEKLVEVAQRPDT 98 ++L ++I + AR+ G II +++GN P IV+ ++ + Sbjct: 8 KKLADVCYDIRGPVLEKARQMEEEGHKIIKLNIGNLAAFGFDAPDEIVQDMIR--NMGNA 65 Query: 99 HGYSSSKGIPRLRRAIAHWYRSRYEVDFDPDSEAIVTIGSKEGLAHLMLATLDKGDTVLV 158 GY+ SKG+ R+++ H Y + +++ + + G+ E + + A L+ GD VLV Sbjct: 66 SGYTDSKGLFAPRKSVMH-YTQQKKIEGVTIDDIYLGNGASELIVMSVNALLNTGDEVLV 124 Query: 159 PNPSYPIHIYGAVIAGANIRSVRMSPGVDFFDELERAVRESYPKPKMMILGFPSNPTAQC 218 P+P YP+ ++G + ++ ++ P K +++ P+NPT Sbjct: 125 PSPDYPLWTAAVSLSGGTPVHYVCDEQQGWQPDIADIKKKITPNTKAIVVINPNNPTGAL 184 Query: 219 VELEFFERVVKLAREHQILVVHDLAYADITFDGWKAPSIMQVPGAREVA-VEFFTLSKSY 277 +E + +++LAR+HQ++++ D Y + +DG S+ + A +V + F LSK+Y Sbjct: 185 YSVEVLKEIIELARQHQLIILADEIYDKVLYDGNTHTSLASL--ADDVLFITFNGLSKNY 242 Query: 278 NMAGWRIGFMVGNARLVAALARIKSYHDYGSF-----TPVQVAAIAALEGDQSCVEEI-- 330 G+R G+MV + A I+ + S P Q A AL G QS + + Sbjct: 243 RSCGYRAGWMVVSGEKKHARDYIEGLNMLASMRLCANAPGQFAIQTALGGYQSINDLVGP 302 Query: 331 RANYERRRNVLVKGLHEA-GWMVDVPKASMYIWARI-PEPYRQFGSLEFARILLEQAKVC 388 + ++R++ + L + G PK+++Y++ R+ PE Y EFA LL + KV Sbjct: 303 QGRLTKQRDLAHQLLTDIPGVSCVKPKSALYMFPRLDPEIYPIKDDQEFAYELLAEEKVL 362 Query: 389 VSPGIGFGEYGDEYVRFALIENESRIRQAI 418 + G GF ++ R + N + +++ Sbjct: 363 IVQGTGFNCPTPDHFRVVFLPNTDDLTESM 392 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.135 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,263,605 Number of extensions: 53832 Number of successful extensions: 200 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 192 Number of HSP's successfully gapped: 11 Length of query: 431 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 343 Effective length of database: 1,203,190 Effective search space: 412694170 Effective search space used: 412694170 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)