BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0015.p (653 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311882|ref|YP_003775974.1| long-chain acyl-CoA synthetase ... 1353 0.0 gi|300313294|ref|YP_003777386.1| long chain acyl-CoA synthetase ... 95 2e-20 gi|300313986|ref|YP_003778078.1| acyl-CoA synthetase/AMP-acid li... 87 5e-18 gi|300313157|ref|YP_003777249.1| long-chain-fatty-acid-CoA ligas... 81 3e-16 gi|300310928|ref|YP_003775020.1| medium-chain-fatty-acid CoA lig... 75 1e-14 >gi|300311882|ref|YP_003775974.1| long-chain acyl-CoA synthetase [Herbaspirillum seropedicae SmR1] Length = 653 Score = 1353 bits (3501), Expect = 0.0, Method: Compositional matrix adjust. Identities = 651/653 (99%), Positives = 653/653 (100%) Query: 1 MVETRQDKEAATFPRLLLAHARQRPERAAFREKDLGIWQTTSWRQVAEEVRSFACGLAAL 60 MVETRQD+EAATFPRLLLAHARQRPERAAFREKDLGIWQTTSWRQVAEEVRSFACGLAAL Sbjct: 1 MVETRQDREAATFPRLLLAHARQRPERAAFREKDLGIWQTTSWRQVAEEVRSFACGLAAL 60 Query: 61 GFRRGMSLAIIGNNCPRLYWAMSAAQALGGMPVPLYQDAPAADMAYVLADANVDFVMAED 120 GFRRGMSLAIIGNNCPRLYWAMSAAQALGGMPVPLYQDAPAADMAYVLADANVDFVMAED Sbjct: 61 GFRRGMSLAIIGNNCPRLYWAMSAAQALGGMPVPLYQDAPAADMAYVLADANVDFVMAED 120 Query: 121 QEQVDKVFEIKESLPRIAHVIYDDERGMRNYHQPELLSFARVQELGRAYERAHPDFFDQE 180 QEQVDKVFEIKESLPRIAHVIYDDERGMRNYHQPELLSFARVQELGRAYERAHPDFFDQE Sbjct: 121 QEQVDKVFEIKESLPRIAHVIYDDERGMRNYHQPELLSFARVQELGRAYERAHPDFFDQE 180 Query: 181 IAAGSPDDVAIILYTSGTTGKPKGVCHSHRAMITTATTLVEFDHLDENDDILCYLPLAWV 240 IAAGSPDDVAIILYTSGTTGKPKGVCHSHRAMITTATTLVEFDHLDENDDILCYLPLAWV Sbjct: 181 IAAGSPDDVAIILYTSGTTGKPKGVCHSHRAMITTATTLVEFDHLDENDDILCYLPLAWV 240 Query: 241 GDFLYSFAQQHVAGFCLNCPESPNTVMTDLREIGPTYYFAPPRVYENILTQVMIRIEDAG 300 GDFLYSFAQQHVAGFCLNCPESPNTVMTDLREIGPTYYFAPPRVYENILTQVMIRIEDAG Sbjct: 241 GDFLYSFAQQHVAGFCLNCPESPNTVMTDLREIGPTYYFAPPRVYENILTQVMIRIEDAG 300 Query: 301 WLKRKMFHGFMKVARRVGMRILDGKPGVSLLDRLQYGLGSLLVYGPLKNVLGMSRLRVAY 360 WLKRKMFHGFMKVARRVGMRILDGKPGVSLLDRLQYGLGSLLVYGPLKNVLGMSRLRVAY Sbjct: 301 WLKRKMFHGFMKVARRVGMRILDGKPGVSLLDRLQYGLGSLLVYGPLKNVLGMSRLRVAY 360 Query: 361 TGGEAIGPDLFDFYRSLGINLKQLYGMTETCVTVCMQPSGDVKLDSVGRPMKGVEVRIDD 420 TGGEAIGPDLFDFYRSLGINLKQLYGMTETCVTVCMQPSGDVKLDSVGRPMKGVEVRID+ Sbjct: 361 TGGEAIGPDLFDFYRSLGINLKQLYGMTETCVTVCMQPSGDVKLDSVGRPMKGVEVRIDE 420 Query: 421 NGEVLVRSPGLMKEYFKRPDATAEAIDANGYFHTGDAGFFDSDGHLKIIDRAKDVGKMAS 480 NGEVLVRSPGLMKEYFKRPDATAEAIDANGYFHTGDAGFFDSDGHLKIIDRAKDVGKMAS Sbjct: 421 NGEVLVRSPGLMKEYFKRPDATAEAIDANGYFHTGDAGFFDSDGHLKIIDRAKDVGKMAS 480 Query: 481 GSMFAPKYIENKLKFFPFIKEAVTFGNGREQCMAFINIDMDAVGNWAERRNLAYSGYTDL 540 GSMFAPKYIENKLKFFPFIKEAVTFGNGREQCMAFINIDMDAVGNWAERRNLAYSGYTDL Sbjct: 481 GSMFAPKYIENKLKFFPFIKEAVTFGNGREQCMAFINIDMDAVGNWAERRNLAYSGYTDL 540 Query: 541 AANPAVYALLRECVEKVNADLAADPLLADSQIHRFLILHKELDPDDEELTRTRKVRRGFI 600 AANPAVYALLRECVEKVNADLAADPLLADSQIHRFLILHKELDPDDEELTRTRKVRRGFI Sbjct: 541 AANPAVYALLRECVEKVNADLAADPLLADSQIHRFLILHKELDPDDEELTRTRKVRRGFI 600 Query: 601 ADKYAVLIEALYAGRASQYIETQVKFEDGRQGMISADLKIAEAKTFKTLQSAA 653 ADKYAVLIEALYAGRASQYIETQVKFEDGRQGMISADLKIAEAKTFKTLQSAA Sbjct: 601 ADKYAVLIEALYAGRASQYIETQVKFEDGRQGMISADLKIAEAKTFKTLQSAA 653 >gi|300313294|ref|YP_003777386.1| long chain acyl-CoA synthetase [Herbaspirillum seropedicae SmR1] Length = 620 Score = 94.7 bits (234), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 111/479 (23%), Positives = 184/479 (38%), Gaps = 92/479 (19%) Query: 175 DFFDQEIAAGSPDDVAIILYTSGTTGKPKGVCHSHRAMITTATTLVE-FDHLDENDDILC 233 D ++ AA P+ + L+TSG+T PK V ++ R M + +V+ F L E IL Sbjct: 197 DEVERAFAATGPETIVKFLFTSGSTKMPKAVINTQRMMCSNLQMIVQTFPFLGEEPPILI 256 Query: 234 -YLPLAWVGDFLYSFAQQHVAGFCL------------NCPESPNTVMTDLREIGPTYYFA 280 +LP + ++F H G L P+ T + +L +I PT YF Sbjct: 257 DWLP------WNHTFGANHNVGIVLYNGGTMYIDEGKPTPQGLATTLANLHDISPTVYFN 310 Query: 281 PPRVYENILTQVMIRIEDAGWLKRKMFHGFMKVARRVGMRILDGKPGVSLLDRLQYGLGS 340 P +E I +E L+ K + Sbjct: 311 VPVGWEGIAHA----LEKDQALREKFY--------------------------------- 333 Query: 341 LLVYGPLKNVLGMSRLRVAYTGGEAIGPDLFDFYRSLG-------INLKQLYGMTETCVT 393 SRLR+ + G A+ ++D + I + GMTET + Sbjct: 334 -------------SRLRMQFYAGAALAQPVWDKLHATAEATCGERIVMCCGLGMTETSPS 380 Query: 394 VCMQPSGDVKLDSVGRPMKGVEVRIDDNG---EVLVRSPGLMKEYFKRPDATAEAIDANG 450 +V+ +G P G+E+++ NG E+ R P + Y++ P+ TAEA D G Sbjct: 381 ALFVADLEVQAGQIGIPTPGMEIKLVPNGDKIEIRYRGPNVTPGYWRAPEQTAEAFDEEG 440 Query: 451 YFHTGDAGFFDSDGH----LKIIDRAKDVGKMASGSMFAPKYIENKLKF--FPFIKEAVT 504 YF +GDA + H R + K+ +G+ + + + P++++AV Sbjct: 441 YFRSGDAVRWLDPAHPDRGFMFDGRVAEDFKLYTGTWVSVGPLRALIAHEGAPYLQDAVI 500 Query: 505 FGNGREQCMAFINIDMDAVGNWAERRNLAYSGYTDLAANPAVYALLRECVEKVNADLAAD 564 G R++ I M V N R LA A A +R+ + V L + Sbjct: 501 TGQDRKE------IGMLIVPNIERCRQLARLPAEASRAEVLNAAPVRDFFQGVLDRLQSH 554 Query: 565 PLLADSQIHRFLILHKELDPDDEELTRTRKVRRGFIADKYAVLIEALYAGRASQYIETQ 623 + +++ R L+L D E+T + + + A L+E LYAG + Q Sbjct: 555 ATGSSNRVTRALVLVDPPSFDRGEITDKGSINQRAVLTHRAALVETLYAGTDPAVLTVQ 613 >gi|300313986|ref|YP_003778078.1| acyl-CoA synthetase/AMP-acid ligase II protein [Herbaspirillum seropedicae SmR1] Length = 546 Score = 86.7 bits (213), Expect = 5e-18, Method: Compositional matrix adjust. Identities = 120/465 (25%), Positives = 193/465 (41%), Gaps = 57/465 (12%) Query: 43 WRQVAEEVRSFACGLAALGFRRGMSLAIIGNNCPRLYWAMSAAQALGGMPVPLYQDAPAA 102 W Q R A L LG + +++ + N P + A G + L A Sbjct: 46 WDQTYRRTRRLASALQGLGVGKNDTVSAMLPNTPAMVEAHFGVPMAGAVLNALNIRLDAE 105 Query: 103 DMAYVLADANVDFVMAEDQEQVDKVFEIKESLPRIAHVIYDDERGMRNYHQPELLSFARV 162 + ++L V+ D E +++ LP + V DE G P ++ R Sbjct: 106 SIVFMLRHGEAK-VLLIDSEFAALAQQLRTQLPALKIVEVFDELG------PPPVAGERF 158 Query: 163 QELG-RAYERAHPDFFDQEIAAGSPDDVAIILYTSGTTGKPKGVCHSHR-AMITTATTLV 220 L A + FD ++ A D +A+ YTSGTTG PKGV + HR A + + ++ Sbjct: 159 GHLEYEALLAGGDEHFDWQMPADEWDAIALN-YTSGTTGDPKGVVYHHRGAALNAVSNIL 217 Query: 221 EFDHLDENDDILCYLPL----AWVGDFLYSFAQQHVAGFCLNCPESPNTVMTDLREIGPT 276 E+D L ++ L LP+ W F ++ A + CL E P V + E G T Sbjct: 218 EWD-LPKHPVYLWTLPMFHCNGWC--FPWTVAARAGVNVCLRKFE-PKLVFDLIAEHGIT 273 Query: 277 YYFAPPRVYENILTQVMIRIEDAGWLKRKMFHGFMKVARRVGMRILDGKPGVSLLDRLQY 336 +Y A P V+ + GW R G +K + P ++L +++ Sbjct: 274 HYCAAPIVHAALANA------PEGW--RAGIRGPVKA------MVAGAPPPAAVLAKME- 318 Query: 337 GLGSLLVYGPLKNVLGMSRLRVAYTGGEAIGPDLF----DFYRSLGINLKQLYGMTETCV 392 + L +V G++ E GP D + +L ++ + + + V Sbjct: 319 -----AMQFELTHVYGLT---------EVYGPAAVCAEQDEWNTLSVDQRAVQ-KSRQGV 363 Query: 393 TVCMQPSGDVKLDSVGRPMKGVEVRIDDNGEVLVRSPGLMKEYFKRPDATAEAIDANGYF 452 +Q SG L M V ++ GE++ R MK Y K AT EA A G+F Sbjct: 364 RYHLQ-SGVTVLSP--DTMAPVAADGEEIGEIMFRGNICMKGYLKNDRATHEAF-AGGWF 419 Query: 453 HTGDAGFFDSDGHLKIIDRAKDVGKMASGSMFAPKYIENKLKFFP 497 HTGD G ++DG++KI DR+KD+ ++ G + +E+ L P Sbjct: 420 HTGDLGVMNADGYIKIKDRSKDI-IISGGENISSVEVEDVLYRHP 463 >gi|300313157|ref|YP_003777249.1| long-chain-fatty-acid-CoA ligase [Herbaspirillum seropedicae SmR1] Length = 566 Score = 80.9 bits (198), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 52/168 (30%), Positives = 80/168 (47%), Gaps = 13/168 (7%) Query: 351 LGMSRLRVAYTGGEAIGPDLFDFYRSL-GINLKQLYGMTETCVTVCMQPSG-DVKLDSVG 408 L S R GG A+ + D ++ L G + + YG++ET P D ++G Sbjct: 325 LDFSGYRCCVGGGMAVQKSVADKWKKLTGCPIIEGYGLSETSPIASANPCTIDEYTGTIG 384 Query: 409 RPMKGVEVRIDDN----------GEVLVRSPGLMKEYFKRPDATAEAIDANGYFHTGDAG 458 P+ E I D+ GE+ +R P +M Y++RPD TA+ + +G+F TGD G Sbjct: 385 LPLPSTEFAILDDDGQPVPLGQPGEIAIRGPQVMPGYWQRPDETAKVMTPDGFFKTGDIG 444 Query: 459 FFDSDGHLKIIDRAKDVGKMASGSMFAPKYIENKLKFFPFIKEAVTFG 506 D G+ KI+DR KD+ + SG P +E + P + E G Sbjct: 445 VMDERGYTKIVDRKKDM-ILVSGFNVYPNEVEGVVAQHPGVLECACVG 491 >gi|300310928|ref|YP_003775020.1| medium-chain-fatty-acid CoA ligase [Herbaspirillum seropedicae SmR1] Length = 545 Score = 75.5 bits (184), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 56/186 (30%), Positives = 92/186 (49%), Gaps = 27/186 (14%) Query: 362 GGEAIGPDLFDFYRSLGINLKQLYGMTETC--VTVCMQPSGDVKLD-------SVGRPMK 412 GG A+ L R GI++ YGM+ETC +T+ P+ ++ + GRP+ Sbjct: 306 GGSAMTGSLAKMARDRGIDVFTGYGMSETCPVLTLAQVPTEELGGEREAAIRVKTGRPLP 365 Query: 413 GVEVRIDD------------NGEVLVRSPGLMKEYFKRPDATAEAIDANGYFHTGDAGFF 460 V++R D GEV+VR+P L + Y + +A+ E + G+ HT D G Sbjct: 366 LVDLRTVDGEMQDCPRDGSTTGEVVVRAPWLTQGYLHQAEASEE-LWRGGWLHTQDIGHL 424 Query: 461 DSDGHLKIIDRAKDVGKMASGSMFAPKYIENKLKFFPFIKEAVTFG--NGR--EQCMAFI 516 D++G+L++ DR KDV K G + IE+ + P + E G +G+ E+ +A + Sbjct: 425 DAEGYLQVTDRIKDVIK-TGGEWVSSLQIEDIIARHPAVAETAVIGIQDGKWGERPVALV 483 Query: 517 NIDMDA 522 + DA Sbjct: 484 VLKKDA 489 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.138 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,062,415 Number of extensions: 89306 Number of successful extensions: 290 Number of sequences better than 1.0e-08: 8 Number of HSP's gapped: 282 Number of HSP's successfully gapped: 9 Length of query: 653 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 562 Effective length of database: 1,188,985 Effective search space: 668209570 Effective search space used: 668209570 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 134 (56.2 bits)