BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0010.p (487 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311886|ref|YP_003775978.1| multi-sensor signal transductio... 974 0.0 gi|300310534|ref|YP_003774626.1| transmembrane sensor histidine ... 134 2e-32 gi|300311282|ref|YP_003775374.1| transmembrane sensor histidine ... 113 4e-26 gi|300312939|ref|YP_003777031.1| transmembrane nitrate/nitrite s... 89 1e-18 gi|300312076|ref|YP_003776168.1| signal transduction histidine k... 64 3e-11 >gi|300311886|ref|YP_003775978.1| multi-sensor signal transduction histidine kinase [Herbaspirillum seropedicae SmR1] Length = 475 Score = 974 bits (2519), Expect = 0.0, Method: Compositional matrix adjust. Identities = 474/475 (99%), Positives = 475/475 (100%) Query: 13 MEEDAFLMVRELKRGGFEPAWERVDDEQALLAALQAQPWDIVISDYSMPMFSGVEALKLV 72 MEEDAFLMVRELKRGGFEPAWERVDDEQALLAALQAQPWDIVISDYSMPMFSGVEALKLV Sbjct: 1 MEEDAFLMVRELKRGGFEPAWERVDDEQALLAALQAQPWDIVISDYSMPMFSGVEALKLV 60 Query: 73 KAHNDQTPFIIVSGVIGEQTAVEVMKAGAQDYFLKSAIGRLPHAVDRELRDAQARRKQRA 132 KAHNDQTPFIIVSGVIGEQTAVEVMKAGAQDYFLKSAIGRLPHAVDRELRDAQARRKQRA Sbjct: 61 KAHNDQTPFIIVSGVIGEQTAVEVMKAGAQDYFLKSAIGRLPHAVDRELRDAQARRKQRA 120 Query: 133 SAQALREMQARFNAFMNAAPMPTWIKDAQLRYSYVNPAQSAFYGITPGDMDGLTDLELMS 192 SAQALREMQARFNAFMNAAPMPTWIKDAQLRYSYVNPAQSAFYG+TPGDMDGLTDLELMS Sbjct: 121 SAQALREMQARFNAFMNAAPMPTWIKDAQLRYSYVNPAQSAFYGMTPGDMDGLTDLELMS 180 Query: 193 SGAAAASQENDRKVLEQKCELHTQETVFDHNHNARILDVVRFPISGEGKGDMVAGLAIDV 252 SGAAAASQENDRKVLEQKCELHTQETVFDHNHNARILDVVRFPISGEGKGDMVAGLAIDV Sbjct: 181 SGAAAASQENDRKVLEQKCELHTQETVFDHNHNARILDVVRFPISGEGKGDMVAGLAIDV 240 Query: 253 TEREQSRRDLELAIQRQQLLSSRVIEVQERERQHLARGLHDDVGQSLTALKINLETIKKM 312 TEREQSRRDLELAIQRQQLLSSRVIEVQERERQHLARGLHDDVGQSLTALKINLETIKKM Sbjct: 241 TEREQSRRDLELAIQRQQLLSSRVIEVQERERQHLARGLHDDVGQSLTALKINLETIKKM 300 Query: 313 GSLEGPSLQNGIDIVTAVLAQVRSLSLDLRPPQLDNLGLIAALRSYVEQKSKLANVNGWF 372 GSLEGPSLQNGIDIVTAVLAQVRSLSLDLRPPQLDNLGLIAALRSYVEQKSKLANVNGWF Sbjct: 301 GSLEGPSLQNGIDIVTAVLAQVRSLSLDLRPPQLDNLGLIAALRSYVEQKSKLANVNGWF 360 Query: 373 ESSGHDEQLHHDIENTAFRIVQEAVTNILRHARCQNIWVKIDRQHDQVYLSIRDDGRGFD 432 ESSGHDEQLHHDIENTAFRIVQEAVTNILRHARCQNIWVKIDRQHDQVYLSIRDDGRGFD Sbjct: 361 ESSGHDEQLHHDIENTAFRIVQEAVTNILRHARCQNIWVKIDRQHDQVYLSIRDDGRGFD 420 Query: 433 IERARSNAISGTSFGLLNMEERAVLVGGSMEITSAPGAGTEIVMHLPVAPIVRRM 487 IERARSNAISGTSFGLLNMEERAVLVGGSMEITSAPGAGTEIVMHLPVAPIVRRM Sbjct: 421 IERARSNAISGTSFGLLNMEERAVLVGGSMEITSAPGAGTEIVMHLPVAPIVRRM 475 >gi|300310534|ref|YP_003774626.1| transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 677 Score = 134 bits (336), Expect = 2e-32, Method: Compositional matrix adjust. Identities = 111/376 (29%), Positives = 183/376 (48%), Gaps = 41/376 (10%) Query: 133 SAQALREMQARFNAFMNAAPMPTWIKDAQLRYSYVNPAQSAFYGITPGDMDGLTDLEL-M 191 +AQ L E A NA + P ++K+ Q + NPA G T + + EL + Sbjct: 310 NAQKLAETSALLNAVSESTSDPIFVKNRQGLMIFANPATLHKLGKTWEETMYRSSRELFL 369 Query: 192 SSGAAAASQENDRKVL----EQKCE--LHTQETVFDHNHNARILDVVRFPISG-EGKGDM 244 A +DR+V+ +K E LH E V + P G +G Sbjct: 370 VPEEADTIDRDDRRVMASGKPEKLEQTLHLPEGVVT-------FQTAKVPWFGKDGSVQG 422 Query: 245 VAGLAIDVTEREQSRRDL--------ELAIQRQQLL---SSRVIEVQERERQHLARGLHD 293 V G++ D+TER+Q+ +L + IQR LL ++ + V+E E++ +AR LHD Sbjct: 423 VIGISTDITERKQAEDELRQRESQLEKTVIQRTALLRELTNHLETVREEEKRAIARELHD 482 Query: 294 DVGQSLTALKINLETIKKMGSLEGPSLQNGID-------IVTAVLAQVRSLSLDLRPPQL 346 ++G SLTAL ++LE + ++ P + D ++ +++A R + +LRP L Sbjct: 483 NMGASLTALSMHLEGVYQIL----PPDEKWADRKVRMQGLMKSLVATTRRIQTELRPNTL 538 Query: 347 DNLGLIAALRSYVEQKSKLANVNGWFESSGHDEQLHHDIENTAFRIVQEAVTNILRHARC 406 D GL AA+ +++ + + D ++ H++E +R++QE + N+ +HA+ Sbjct: 539 DLFGLKAAISEQLDELHERTGIACHASLPDEDVEVGHEMEIAIYRMLQEMLNNVTKHAKA 598 Query: 407 QNIWVKIDRQHDQVYLSIRDDGRGFDIERARSNAISGTSFGLLNMEERAVLVGGSMEITS 466 + V +D D V L++RDDG G ER R N ++GL + ERA GG ++I S Sbjct: 599 SKVDVILDIDEDHVALTVRDDGVGIPEER-RDNH---KTYGLRGLRERATFFGGEVDIRS 654 Query: 467 APGAGTEIVMHLPVAP 482 PG G I + LP+ P Sbjct: 655 TPGKGALITISLPIRP 670 >gi|300311282|ref|YP_003775374.1| transmembrane sensor histidine kinase transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 464 Score = 113 bits (282), Expect = 4e-26, Method: Compositional matrix adjust. Identities = 82/215 (38%), Positives = 118/215 (54%), Gaps = 15/215 (6%) Query: 272 LSSRVIEVQERERQHLARGLHDDVGQSLTALKINLET--IKKMGSLEGPS-----LQNGI 324 L+ RV+ QE ER L+R LHD + Q L ++K+ +E ++ G+ E + + Sbjct: 238 LAQRVVRSQEEERARLSRDLHDGISQWLVSIKLQIEAALVRLNGTPEQAATARAGFEKTA 297 Query: 325 DIVTAVLAQVRSLSLDLRPPQLDNLGLIAALRSYVEQ--KSKLANVNGWFESSGH--DEQ 380 + VLA+VR +S DLRP LD+LGL AAL + + A V FE+ D Sbjct: 298 TQINGVLAEVRRISHDLRPAILDDLGLAAALEHLCGEWRDAAGAGVEIVFENQAQADDGA 357 Query: 381 LHHDIENTA-FRIVQEAVTNILRHARCQNIWVKIDRQHDQVYLSIRDDGRGFDIERARSN 439 D+ NT FRI QEA+TNI RHA I V++ + H+ + L ++D+G GFD+ + + Sbjct: 358 TLPDVVNTVLFRIAQEALTNIARHAGASRIKVRLYQGHNLLMLQVQDNGHGFDLGQVDDH 417 Query: 440 AISGTSFGLLNMEERAVLVGGSMEITSAPGAGTEI 474 + G GL NM ER VGG + I S+P AGT I Sbjct: 418 SRHG--IGLRNMRERIEAVGGELGIDSSP-AGTSI 449 >gi|300312939|ref|YP_003777031.1| transmembrane nitrate/nitrite sensor histidine kinase transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 650 Score = 88.6 bits (218), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 68/227 (29%), Positives = 115/227 (50%), Gaps = 15/227 (6%) Query: 260 RDLELAIQRQQLLSSRVIEVQERERQHLARGLHDDVGQSLTALKINLETIKKMGSLE--- 316 R L AI+ Q+L++ ER LA+GLHD + Q L L + ++ ++ + + Sbjct: 423 RHLGSAIENQRLIAREKEFAIANERNLLAQGLHDSIAQGLNFLNLQVQMLEDSLARDRLD 482 Query: 317 -----GPSLQNGIDIVTAVLAQVRSLSLDLRPPQLDNLGLIAALRSYVEQKSKLANVNGW 371 P L+ G V VR L L+ R +L + L + +R +++ + + + G Sbjct: 483 EIRDIAPLLRTG---VQENYEDVRELLLNFRT-RLQDSNLDSEMRKALDKLQRQSGLQGQ 538 Query: 372 FESSGHDEQLHHDIENTAFRIVQEAVTNILRHARCQNIWVKIDRQHDQVYLSIRDDGRGF 431 +G L + + I+QEA++NI +HAR Q + + +D + D +++ DDG GF Sbjct: 539 ITLTGDGPPLAPEQQLQVLFILQEALSNIRKHARAQQVQISVDNRQD-FRMTVSDDGHGF 597 Query: 432 DIERARSNAISGTSFGLLNMEERAVLVGGSMEITSAPGAGTEIVMHL 478 +E AR+ + GL M+ERA +G ++EI S PG GT I + L Sbjct: 598 TLESARAKGLE--HVGLRIMQERAERLGANLEIDSQPGHGTRIALTL 642 >gi|300312076|ref|YP_003776168.1| signal transduction histidine kinase [Herbaspirillum seropedicae SmR1] Length = 356 Score = 63.5 bits (153), Expect = 3e-11, Method: Compositional matrix adjust. Identities = 57/210 (27%), Positives = 99/210 (47%), Gaps = 7/210 (3%) Query: 277 IEVQERERQHLARGLHDDVGQSLTALKINLETIKKMGSLEG-----PSLQNGIDIVTAVL 331 I ++E+ER+ +AR LHDD+G L + + L +++ + +G ++ + Sbjct: 148 IHIREQERERIARDLHDDLGGVLAGVGMALGRLQEQLAAQGLAAPLAEADEARQLLQQAM 207 Query: 332 AQVRSLSLDLRPPQLDNLGLIAALRSYVEQKSKLANVNGWFESSGHDEQLHHDIENTAFR 391 A + S+ +L PP ++ GL+ AL+ Q + ++ + EQ + R Sbjct: 208 ASMHSIIDELHPPIVE-FGLVDALQWQCRQIQREYRLHCQLDCPPTLEQEDRFLILGLLR 266 Query: 392 IVQEAVTNILRHARCQNIWVKIDRQHDQVYLSIRDDGRGFDIERARSNAISGTSFGLLNM 451 I++EA +N RH R + V + + ++ + I DDG G A+ A GL NM Sbjct: 267 ILREAASNAARHGRASALRVDLRMRQARLEMQIADDGCGIAPLHAKEVA-PRHGHGLRNM 325 Query: 452 EERAVLVGGSMEITSAPGAGTEIVMHLPVA 481 ERA +GG + I + G I +PVA Sbjct: 326 RERAQALGGELFIEAGQDGGVVIRTLIPVA 355 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.134 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,386,925 Number of extensions: 53303 Number of successful extensions: 291 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 290 Number of HSP's successfully gapped: 5 Length of query: 487 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 398 Effective length of database: 1,198,455 Effective search space: 476985090 Effective search space used: 476985090 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)