BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0030.0008.p
         (185 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300311888|ref|YP_003775980.1| signal peptide protein [Herbasp...   253   5e-69
gi|300311892|ref|YP_003775984.1| signal peptide protein [Herbasp...    88   5e-19

>gi|300311888|ref|YP_003775980.1| signal peptide protein
           [Herbaspirillum seropedicae SmR1]
          Length = 159

 Score =  253 bits (647), Expect = 5e-69,   Method: Compositional matrix adjust.
 Identities = 141/144 (97%), Positives = 141/144 (97%)

Query: 1   MTMKTSPLLLLLAALPLAGIGNALAAAPTGNLEISAIVTQQCSVQSAALPFGVYGSSALQ 60
           MTMKTSPLLLLLAALPLAGIGNALAAAPTGNLEISAIVTQQCSVQSAALPFGVYGSSALQ
Sbjct: 1   MTMKTSPLLLLLAALPLAGIGNALAAAPTGNLEISAIVTQQCSVQSAALPFGVYGSSALQ 60

Query: 61  QNAQIGVTCSNGTSYTVGLDQGAAGSANRRMASADGASWIDYGLYQDAARTTAWNTGSAA 120
           QNAQIGVTCSNGTSYTVGLDQGAAGSANRRMASADGASWIDYGLYQDAARTTAWNTGSAA
Sbjct: 61  QNAQIGVTCSNGTSYTVGLDQGAAGSANRRMASADGASWIDYGLYQDAARTTAWNTGSAA 120

Query: 121 VAGVGTGNAQNFPVYGYVPAVSRP 144
           VAGVGTGNAQNFPVYGYVPA   P
Sbjct: 121 VAGVGTGNAQNFPVYGYVPAGQSP 144


>gi|300311892|ref|YP_003775984.1| signal peptide protein
           [Herbaspirillum seropedicae SmR1]
          Length = 166

 Score = 87.8 bits (216), Expect = 5e-19,   Method: Compositional matrix adjust.
 Identities = 49/119 (41%), Positives = 70/119 (58%), Gaps = 6/119 (5%)

Query: 32  LEISAIVTQQCSVQSAALPFGVYGSSALQQNAQIGVTCSNGTSYTVGLDQGAAGSA---N 88
           L ISA V   C+V  +++ FG Y  + + Q   I V C+NGT+Y VGLD G    A   N
Sbjct: 35  LTISASVVASCTVVGSSIAFGAYTQAVVNQTGSITVQCTNGTAYNVGLDAGTGTGATVSN 94

Query: 89  RRMASADGASWIDYGLYQDAARTTAWNT--GSAAVAGVGTGNAQNFPVYGYVPAVSRPV 145
           R+M SA G   ++Y LY+D+AR++ W +  G+  + G G+G AQ   VYG +PA   P+
Sbjct: 95  RKM-SASGGGTLNYALYRDSARSSNWGSTIGTDTLTGTGSGLAQTLTVYGQIPAAQTPL 152


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.313    0.125    0.359 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 342,331
Number of extensions: 10556
Number of successful extensions: 50
Number of sequences better than 1.0e-08: 3
Number of HSP's gapped: 49
Number of HSP's successfully gapped: 3
Length of query: 185
Length of database: 1,619,870
Length adjustment: 80
Effective length of query: 105
Effective length of database: 1,241,070
Effective search space: 130312350
Effective search space used: 130312350
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 128 (53.9 bits)