BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0029.0361.p (457 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311130|ref|YP_003775222.1| Type I antifreeze protein:HlyD ... 905 0.0 gi|300312349|ref|YP_003776441.1| hemolysin-type secretion transm... 182 6e-47 gi|300312188|ref|YP_003776280.1| hemolysin secretion-related tra... 108 7e-25 >gi|300311130|ref|YP_003775222.1| Type I antifreeze protein:HlyD family secretion protein [Herbaspirillum seropedicae SmR1] Length = 457 Score = 905 bits (2340), Expect = 0.0, Method: Compositional matrix adjust. Identities = 456/457 (99%), Positives = 456/457 (99%) Query: 1 MTLSLKPNATHGIGARGQGFMRRLAPRLDRRLGELATMPRPRLAVLAVTVLLALLVLWSV 60 MTLSLKPNAT GIGARGQGFMRRLAPRLDRRLGELATMPRPRLAVLAVTVLLALLVLWSV Sbjct: 1 MTLSLKPNATPGIGARGQGFMRRLAPRLDRRLGELATMPRPRLAVLAVTVLLALLVLWSV 60 Query: 61 FAPIDMVVRGSGRIVASEHNQVIQHLEGGIVSAILVREGAAVKKGQTLISISDVRANADL 120 FAPIDMVVRGSGRIVASEHNQVIQHLEGGIVSAILVREGAAVKKGQTLISISDVRANADL Sbjct: 61 FAPIDMVVRGSGRIVASEHNQVIQHLEGGIVSAILVREGAAVKKGQTLISISDVRANADL 120 Query: 121 GEGRVKILGLRAKIARLSAEASGASSLQMPDDLERGDPAVQSEQAAFAARSSKLMQEMSV 180 GEGRVKILGLRAKIARLSAEASGASSLQMPDDLERGDPAVQSEQAAFAARSSKLMQEMSV Sbjct: 121 GEGRVKILGLRAKIARLSAEASGASSLQMPDDLERGDPAVQSEQAAFAARSSKLMQEMSV 180 Query: 181 LREQSAQRQAELGEAISRQKSQASELELAQRQYKLIHDMLVRNAASQLEDIQAASRVQDA 240 LREQSAQRQAELGEAISRQKSQASELELAQRQYKLIHDMLVRNAASQLEDIQAASRVQDA Sbjct: 181 LREQSAQRQAELGEAISRQKSQASELELAQRQYKLIHDMLVRNAASQLEDIQAASRVQDA 240 Query: 241 QSKLAATNAMIPRLNAAIAEAQAKQGEAASRFRSDARTELTAAETELARLQEEIKSRNDR 300 QSKLAATNAMIPRLNAAIAEAQAKQGEAASRFRSDARTELTAAETELARLQEEIKSRNDR Sbjct: 241 QSKLAATNAMIPRLNAAIAEAQAKQGEAASRFRSDARTELTAAETELARLQEEIKSRNDR 300 Query: 301 VSRSEVKAPMDGVVNRVMINTIGGVVRPGDPIIEMTPSDDKLVIEARISPTDRAELVTGA 360 VSRSEVKAPMDGVVNRVMINTIGGVVRPGDPIIEMTPSDDKLVIEARISPTDRAELVTGA Sbjct: 301 VSRSEVKAPMDGVVNRVMINTIGGVVRPGDPIIEMTPSDDKLVIEARISPTDRAELVTGA 360 Query: 361 RARVKVSAYDYGVYGAMDGVVTEISADTVPDEGSPRGERFYRLKIEVKRGHDELQQKLAL 420 RARVKVSAYDYGVYGAMDGVVTEISADTVPDEGSPRGERFYRLKIEVKRGHDELQQKLAL Sbjct: 361 RARVKVSAYDYGVYGAMDGVVTEISADTVPDEGSPRGERFYRLKIEVKRGHDELQQKLAL 420 Query: 421 MPGMTASADIIVGRRTVWQYLMSPLSRFGQGALREPR 457 MPGMTASADIIVGRRTVWQYLMSPLSRFGQGALREPR Sbjct: 421 MPGMTASADIIVGRRTVWQYLMSPLSRFGQGALREPR 457 >gi|300312349|ref|YP_003776441.1| hemolysin-type secretion transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 386 Score = 182 bits (461), Expect = 6e-47, Method: Compositional matrix adjust. Identities = 133/420 (31%), Positives = 210/420 (50%), Gaps = 58/420 (13%) Query: 42 RLAVLAVTVLLALLVLWSVFAPIDMVVRGSGRIVASEHNQVIQHLEGGIVSAILVREGAA 101 RL++ +V VLL LW+ + ID V R G+++A Q +Q +GG V ILV+EG A Sbjct: 18 RLSMASVLVLL----LWAAVSQIDQVTRAQGQVIAVSRTQSVQAPDGGPVKQILVKEGEA 73 Query: 102 VKKGQTLISISDVRANADLGEGRVKILGLRAKIARLSAEASGASSLQMPDDLERGDPAVQ 161 VK+GQ L+ + R L + R K+ LR +ARL AE G PD L+ + Sbjct: 74 VKQGQLLMVLEQDRVQTSLNDSRAKVAALRISLARLRAEVYGHPLNFEPDLLDYKE---- 129 Query: 162 SEQAAFAARSSKLMQEMSVLREQSAQRQAELGEAISRQKSQASELELAQRQYKLIHDMLV 221 + A L + +L +Q +LA Q DML Sbjct: 130 -----YIANQKDLYVKRKILIDQ----------------------DLASLQ-----DMLG 157 Query: 222 RNAASQLEDIQAASRVQDAQSKLAATNAMIPRLNAAIAEAQAKQGEAASRFRSDARTELT 281 A +LE ++ R D A + RL +A+ +A+ +++ +A ++T Sbjct: 158 L-ANQELEMNKSLERTGDV------GRADVLRLQRNVADIRAQINNKRNKYFQEALVDMT 210 Query: 282 AAETELARLQEEIKSRNDRVSRSEVKAPMDGVVNRVMINTIGGVVRPGDPIIEMTPSDDK 341 + +L +E+++ R + +E+KAP DGVV + + TIGGVVR GD ++E+ P D+ Sbjct: 211 KVQEDLNTQREQLEDRTQVLEHTELKAPADGVVKNIKVTTIGGVVRSGDVVMEILPVDN- 269 Query: 342 LVIEARISPTDRAELVTGARARVKVSAYDYGVYGAMDGVVTEISADTVPDEGSPRGERFY 401 L++EA++S D A + G A VK+ AYDY +YG ++G V +S DT+ DE +Y Sbjct: 270 LIVEAKVSTADSAYVKVGQAANVKLDAYDYSIYGTLNGHVVYVSPDTLLDETRQGAMPYY 329 Query: 402 RLKIEVK------RGHDELQQKLALMPGMTASADIIVGRRTVWQYLMSPLSRFGQGALRE 455 R+ I ++ R D +Q K PGMTA+ +I RTV +L P+++ Q ++ E Sbjct: 330 RVHIALEEAVFKGRKADAIQVK----PGMTATVEIKANTRTVLSFLTKPIAKTLQESMGE 385 >gi|300312188|ref|YP_003776280.1| hemolysin secretion-related transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 447 Score = 108 bits (271), Expect = 7e-25, Method: Compositional matrix adjust. Identities = 116/411 (28%), Positives = 195/411 (47%), Gaps = 39/411 (9%) Query: 50 VLLALLVLWSVFAPIDMVVRGSGRIVASEHNQVIQHLEGGIVSAILVREGAAVKKGQTLI 109 V+ ALL WS+ ID+VV +G+++ S + I ++ V AILV EG VK G+ LI Sbjct: 70 VIFALL--WSLLGRIDIVVSANGKVIPSARTKTIASVDVSAVRAILVSEGQLVKAGEVLI 127 Query: 110 SISDVRANADLGEGRVKILGLRAKIARLSAEASGASSLQMPDDLERGDPAVQSEQAAFAA 169 + ++A+ + + R + AR A +L+ P+ + P V QA + A Sbjct: 128 ELDSSSSDAERDKAADAVAQARLQAARAQAMMEAIQNLRTPELAQV--PGV--AQAQWEA 183 Query: 170 RSSKLMQEMSVLREQSAQRQAELGEAISRQKSQASELELAQRQ---YK-LIHDMLVRNAA 225 S+L+ + R R L + ISR + L+LA ++ Y+ L+ D V A Sbjct: 184 VRSQLVAQYQDFRA----RLGRLDDEISR---YGAALKLATQRASDYQTLVADHTVSRHA 236 Query: 226 SQLEDIQAASRVQDAQSKLAATNAMIPRLNAAIAEAQAKQGEAASRFRSDARTELTAAET 285 LE QA RV L + +A+ ++ ++ R DA A+ Sbjct: 237 -WLEKEQA--RVD---------------LQGQLDDARNQRATLVAQTRKDAHDSRIEADK 278 Query: 286 ELARLQEEIKSRNDRVSRSEVKAPMDGVVNRVMINTIGGVVRPGDPIIEMTPSDDKLVIE 345 + Q++ + ++ ++ AP+ G V ++ ++TIGGVV P++++ P D ++ +E Sbjct: 279 TIEASQQDQRRADEHSKLLKLVAPVTGTVQQLTVHTIGGVVPAAQPLMQIVPQDSQIEVE 338 Query: 346 ARISPTDRAELVTGARARVKVSAYDYGVYGAMDGVVTEISADTVPDEGSPRGERFYRLKI 405 A + D + G A VK+ A+DY YG + V IS D + DE + Y +I Sbjct: 339 AMLENKDVGFVEIGQAAEVKIDAFDYTKYGTLPARVVHISRDAIQDE---KKGLLYSARI 395 Query: 406 EVKRGHDELQ-QKLALMPGMTASADIIVGRRTVWQYLMSPLSRFGQGALRE 455 + + + +K+ L GM A+ +I G R V +Y +SPL R + AL E Sbjct: 396 VLDQSSLIVDGRKVQLSAGMAANVEIKTGNRRVIEYALSPLIRHQKEALHE 446 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.130 0.351 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,253,412 Number of extensions: 48247 Number of successful extensions: 312 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 311 Number of HSP's successfully gapped: 5 Length of query: 457 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 369 Effective length of database: 1,203,190 Effective search space: 443977110 Effective search space used: 443977110 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)