BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0029.0350.p (274 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311120|ref|YP_003775212.1| metal ion ABC transporter perip... 548 e-157 gi|300310325|ref|YP_003774417.1| metal ion ABC transporter perip... 275 3e-75 gi|300314030|ref|YP_003778122.1| metal ion ABC transporter perip... 273 1e-74 gi|300310887|ref|YP_003774979.1| metal ion ABC transporter perip... 189 3e-49 gi|300311637|ref|YP_003775729.1| metal ion ABC transporter perip... 160 1e-40 >gi|300311120|ref|YP_003775212.1| metal ion ABC transporter periplasmic component/surface antigen protein [Herbaspirillum seropedicae SmR1] Length = 274 Score = 548 bits (1412), Expect = e-157, Method: Compositional matrix adjust. Identities = 273/274 (99%), Positives = 273/274 (99%) Query: 1 MHFASCFKNIKRLAAIGALSLALVPQLVQAQDKQKIKVGISVGSAEAIFDVVKKVAARDG 60 MHFASCFKNIKRLAAIGALSLALVPQL QAQDKQKIKVGISVGSAEAIFDVVKKVAARDG Sbjct: 1 MHFASCFKNIKRLAAIGALSLALVPQLGQAQDKQKIKVGISVGSAEAIFDVVKKVAARDG 60 Query: 61 LEIQTVVFSDYLQPNAALAAGDLDANAFQHRPYLESQIAARGYRIVPVGLTITAPLGIYS 120 LEIQTVVFSDYLQPNAALAAGDLDANAFQHRPYLESQIAARGYRIVPVGLTITAPLGIYS Sbjct: 61 LEIQTVVFSDYLQPNAALAAGDLDANAFQHRPYLESQIAARGYRIVPVGLTITAPLGIYS 120 Query: 121 RKFRSVEQLPQGAAIGIQNDPSNGNRALLLLQKAGLIRLKPGVGENGVNATPLDVIENPR 180 RKFRSVEQLPQGAAIGIQNDPSNGNRALLLLQKAGLIRLKPGVGENGVNATPLDVIENPR Sbjct: 121 RKFRSVEQLPQGAAIGIQNDPSNGNRALLLLQKAGLIRLKPGVGENGVNATPLDVIENPR 180 Query: 181 KLKLVELDAAQLPRSLDDLAAASINNDYAFKAGLSLQKDTIAVEDPRGRYANIIATRAED 240 KLKLVELDAAQLPRSLDDLAAASINNDYAFKAGLSLQKDTIAVEDPRGRYANIIATRAED Sbjct: 181 KLKLVELDAAQLPRSLDDLAAASINNDYAFKAGLSLQKDTIAVEDPRGRYANIIATRAED 240 Query: 241 KDRPWVRKLVQAYHSEEVRKFIETEFKGSLVPAF 274 KDRPWVRKLVQAYHSEEVRKFIETEFKGSLVPAF Sbjct: 241 KDRPWVRKLVQAYHSEEVRKFIETEFKGSLVPAF 274 >gi|300310325|ref|YP_003774417.1| metal ion ABC transporter periplasmic component/surface antigen protein [Herbaspirillum seropedicae SmR1] Length = 265 Score = 275 bits (702), Expect = 3e-75, Method: Compositional matrix adjust. Identities = 146/265 (55%), Positives = 200/265 (75%), Gaps = 6/265 (2%) Query: 10 IKRLAAIGALSLALVPQLVQAQDKQKIKVGISVGSAEAIFDVVKKVAARDGLEIQTVVFS 69 I+ +A +G L+ LV AQD+ IK+G++ G I + VKK+ +DG++++ + F+ Sbjct: 7 IQFIAGLG-LAAGLVSAPAFAQDQ--IKMGVTAGPHAEIMEQVKKLLEKDGVQMKVIEFT 63 Query: 70 DYLQPNAALAAGDLDANAFQHRPYLESQIAARGYRIVPVGLTITAPLGIYSRKFRSVEQL 129 DY+QPNAALAAGDLDAN++QH+PYL++QI RGY+ V VG TIT P+G+YS+K +S+ L Sbjct: 64 DYIQPNAALAAGDLDANSYQHQPYLDAQIKDRGYKFVSVGSTITFPMGVYSKKIKSLNDL 123 Query: 130 PQGAAIGIQNDPSNGNRALLLLQKAGLIRLKPGVGENGVNATPLDVIENPRKLKLVELDA 189 QGA +G+ NDP+NG RALL+LQ G+I+LK + G+ ATPLD++ENP+K+K+VELDA Sbjct: 124 KQGARVGVPNDPTNGGRALLVLQAKGVIKLK---ADAGLKATPLDIVENPKKIKIVELDA 180 Query: 190 AQLPRSLDDLAAASINNDYAFKAGLSLQKDTIAVEDPRGRYANIIATRAEDKDRPWVRKL 249 AQLPRSLDD AA IN +YA AGLS KD IAVE G YAN+IA R DKD+PWV KL Sbjct: 181 AQLPRSLDDFDAAVINGNYAESAGLSPTKDAIAVEASTGPYANVIAVRIADKDKPWVAKL 240 Query: 250 VQAYHSEEVRKFIETEFKGSLVPAF 274 V+AYHS EV+KF+ ++KGS++ ++ Sbjct: 241 VKAYHSPEVKKFVLEKYKGSVITSW 265 >gi|300314030|ref|YP_003778122.1| metal ion ABC transporter periplasmic component/surface antigen protein [Herbaspirillum seropedicae SmR1] Length = 276 Score = 273 bits (698), Expect = 1e-74, Method: Compositional matrix adjust. Identities = 134/268 (50%), Positives = 191/268 (71%), Gaps = 3/268 (1%) Query: 10 IKRLAAIGALSLALVPQLVQAQDKQKIKVGISVGSAEAIFDVVKKVAARD--GLEIQTVV 67 ++RL+ + LA + QA Q I+VG++ G IF+ ++V RD G +++ + Sbjct: 9 LRRLSTLMVSMLASLVLNAQAAADQVIRVGVTSGPHAQIFEQARRVFERDNPGYKVKIIE 68 Query: 68 FSDYLQPNAALAAGDLDANAFQHRPYLESQIAARGYRIVPVGLTITAPLGIYSRKFRSVE 127 F+DY+QPNAAL AG+LDAN++QHRP+L +QI RGY++ G T+ P+ IYSRK+R +E Sbjct: 69 FNDYIQPNAALDAGELDANSYQHRPFLNAQIKTRGYKLYAEGKTMIGPMAIYSRKYRKLE 128 Query: 128 QLPQGAAIGIQNDPSNGNRALLLLQKAGLIRLKPGVGE-NGVNATPLDVIENPRKLKLVE 186 +P GA IGI NDP+N +R LLLLQK G I+L+ G+ G NATP+D+ ENP+K K VE Sbjct: 129 DVPVGARIGIPNDPANESRVLLLLQKHGFIKLRAGIDPLTGTNATPIDITENPKKWKFVE 188 Query: 187 LDAAQLPRSLDDLAAASINNDYAFKAGLSLQKDTIAVEDPRGRYANIIATRAEDKDRPWV 246 +DAAQLPR+LDDL A+++N DYA KAGL+ +D++ VE YA +IA R +D+ +PW+ Sbjct: 189 IDAAQLPRTLDDLDASAVNADYAAKAGLNPARDSLVVESGDSPYACLIAVREKDRAQPWL 248 Query: 247 RKLVQAYHSEEVRKFIETEFKGSLVPAF 274 KLVQAY S EV+++IETEFKG ++ A+ Sbjct: 249 GKLVQAYQSPEVKRYIETEFKGGILAAW 276 >gi|300310887|ref|YP_003774979.1| metal ion ABC transporter periplasmic component/surface antigen protein [Herbaspirillum seropedicae SmR1] Length = 263 Score = 189 bits (479), Expect = 3e-49, Method: Compositional matrix adjust. Identities = 96/228 (42%), Positives = 144/228 (63%), Gaps = 4/228 (1%) Query: 48 IFDVVKKVAARDGLEIQTVVFSDYLQPNAALAAGDLDANAFQHRPYLESQIAARGYRI-V 106 I + +K A++G+++Q VF+DY+QP A +D N F H+PYL+ + I V Sbjct: 39 ILEHIKPALAKEGIDLQVKVFTDYVQPAAQTNEKQVDGNFFLHQPYLDQFKKSHKNDIEV 98 Query: 107 PVGLTITAPLGIYSRKFRSVEQLPQGAAIGIQNDPSNGNRALLLLQKAGLIRLKPGVGEN 166 PV P YS+K++ + +P GA I I NDPSN RALLLL + GLI+LK Sbjct: 99 PVAKVHVEPFAAYSQKYKKIADIPNGATIAIPNDPSNSGRALLLLARNGLIKLK---DNT 155 Query: 167 GVNATPLDVIENPRKLKLVELDAAQLPRSLDDLAAASINNDYAFKAGLSLQKDTIAVEDP 226 ++AT D++ENP+KLK L+AA LPR L+ + A IN +YA +A L+ KD++ +ED Sbjct: 156 NISATQKDIVENPKKLKFRALEAATLPRVLNQVDVALINTNYALEAKLNPVKDSLFIEDA 215 Query: 227 RGRYANIIATRAEDKDRPWVRKLVQAYHSEEVRKFIETEFKGSLVPAF 274 YAN++ R ++KD P ++KL A +S +V+KFIE +++G++VPAF Sbjct: 216 NSPYANLLVAREDNKDSPAIKKLAAALNSPDVKKFIEEKYQGAIVPAF 263 >gi|300311637|ref|YP_003775729.1| metal ion ABC transporter periplasmic component/surface antigen protein [Herbaspirillum seropedicae SmR1] Length = 268 Score = 160 bits (405), Expect = 1e-40, Method: Compositional matrix adjust. Identities = 106/256 (41%), Positives = 146/256 (57%), Gaps = 18/256 (7%) Query: 19 LSLALVPQLVQAQDKQKIKVGISVGSAEAIFDVVKKVAARDGLEIQTVVFSDYLQPNAAL 78 LSL ++ L AQ K+++G+ G+ V K A R G+++Q V F+D+ PN AL Sbjct: 9 LSLTVL-SLQAAQAADKLRIGVVPGAYGDSVAVAAKEAKRQGIDVQVVEFTDWTTPNVAL 67 Query: 79 AAGDLDANAFQHRPYLESQIAARGYRIVPVGLTITAPLGIYSRKFRSVEQLPQGAAIGIQ 138 AGDLD N FQH+P+L++ I GY++ G I + +G+YS K++S+ LPQGA +GI Sbjct: 68 DAGDLDLNYFQHQPFLDNAIQKNGYKLASAGTGILSNVGLYSLKYKSLTALPQGAKVGIA 127 Query: 139 NDPSNGNRALLLLQKAGLIRLKPGVGENGVNATPLDVIENPRKLKLVELDAAQLPRSLDD 198 NDP N RALLLLQ GLI+LKP VG G + D+I+NPRKL VE++ QL R D Sbjct: 128 NDPVNQGRALLLLQNVGLIKLKPKVGYLG---SIDDIIDNPRKLSFVEVEGPQLVRITPD 184 Query: 199 LAAAS-----INNDYAF--KAGLSLQKDTIAVEDPRGRYANIIATRAEDKDRPWVRKLVQ 251 + A I AF +GL+ +ED R+A TRA + P V+K +Q Sbjct: 185 VDLAQGYPHFIVAAKAFDPSSGLAYS----GIED--ARFAIQFVTRANRVNDPVVQKFIQ 238 Query: 252 AY-HSEEVRKFIETEF 266 Y +S VR I F Sbjct: 239 IYQNSAAVRADIGRAF 254 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.135 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 773,408 Number of extensions: 30588 Number of successful extensions: 125 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 118 Number of HSP's successfully gapped: 5 Length of query: 274 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 190 Effective length of database: 1,222,130 Effective search space: 232204700 Effective search space used: 232204700 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)