BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0029.0329.p (772 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311101|ref|YP_003775193.1| NADH dehydrogenase I subunit G ... 1573 0.0 gi|300310808|ref|YP_003774900.1| NAD-dependent formate dehydroge... 220 3e-58 >gi|300311101|ref|YP_003775193.1| NADH dehydrogenase I subunit G [Herbaspirillum seropedicae SmR1] Length = 772 Score = 1573 bits (4074), Expect = 0.0, Method: Compositional matrix adjust. Identities = 767/772 (99%), Positives = 771/772 (99%) Query: 1 MVEIEIDGKKVEVQEGSMVMDAANKLGTYIPHFCYHKKLSIAANCRMCLVEVEKAPKPLP 60 MVEIEIDGKKVEVQEGSMVMDAANKLGTYIPHFCYHKKLSIAANCRMCLVEVEKAPKPLP Sbjct: 1 MVEIEIDGKKVEVQEGSMVMDAANKLGTYIPHFCYHKKLSIAANCRMCLVEVEKAPKPLP 60 Query: 61 ACATPVNAGMIVRTASDKAVKAQKGVMEFLLINHPLDCPICDQGGECQLQDLAVGYGGSS 120 ACATPVNAGMIVRTASDKAVKAQKGVMEFLLINHPLDCPICDQGGECQLQDLAVGYGGSS Sbjct: 61 ACATPVNAGMIVRTASDKAVKAQKGVMEFLLINHPLDCPICDQGGECQLQDLAVGYGGSS 120 Query: 121 SRYQEEKRVVEPKDVGPLISMKEMNRCIHCTRCVRFGQEVAGVMELGMLGRGEHSEITTF 180 SRYQEEKRVVEPKDVGPLISMKEMNRCIHCTRCVRFGQEVAGVMELGMLGRGEHSEITTF Sbjct: 121 SRYQEEKRVVEPKDVGPLISMKEMNRCIHCTRCVRFGQEVAGVMELGMLGRGEHSEITTF 180 Query: 181 VGKTVDSELSGNMIDLCPVGALTSKPFRYSARTWELSRRKSVSPHDSVGANLIVQVKNGK 240 VGKTVDSELSGNMIDLCPVGALTSKPFRYSARTWELSRRKSVSPHDSVGANLIVQVKNGK Sbjct: 181 VGKTVDSELSGNMIDLCPVGALTSKPFRYSARTWELSRRKSVSPHDSVGANLIVQVKNGK 240 Query: 241 VMRVLPFENEEVNECWISDKDRFAYEALDSEQRLTKPMLKQDGKWQEVDWQTALEYVAHG 300 VMRVLPFENEEVNECWISDKDRFAYEALDSEQRLTKPMLKQDGKWQEVDWQTALEYVAHG Sbjct: 241 VMRVLPFENEEVNECWISDKDRFAYEALDSEQRLTKPMLKQDGKWQEVDWQTALEYVAHG 300 Query: 301 LRNIRHEHGADAIAALGTPYSTLEELSLLQKIVRGVGSDNVDFRLRQSDFGLDGQIKPWL 360 LRNIRHEHGADAIAALGTPYSTLEELSLLQKIVRGVGS+N+DFRLRQSDFGLDGQIKPWL Sbjct: 301 LRNIRHEHGADAIAALGTPYSTLEELSLLQKIVRGVGSENIDFRLRQSDFGLDGQIKPWL 360 Query: 361 GMSIVEFSQLNRVFVIGSFLRKDHPLLSARLRQSAKSGAKVSVLHATDDDLLMPVANKII 420 GMSIVEFSQLNRVFVIGSFLRKDHPLLSARLRQSAKSGAKVSVLHATDDDLLMPVANKII Sbjct: 361 GMSIVEFSQLNRVFVIGSFLRKDHPLLSARLRQSAKSGAKVSVLHATDDDLLMPVANKII 420 Query: 421 AAPSAWLSALAEVAVAVAEAKGVAAPSAYAGVTASDAAKATAASLLSGDAKGVFLGNAAV 480 AAPSAWLSALAEVAVAVAEAKGVAAPSAYAGVTASDAAKATAASLLSGDAKGVFLGNAAV Sbjct: 421 AAPSAWLSALAEVAVAVAEAKGVAAPSAYAGVTASDAAKATAASLLSGDAKGVFLGNAAV 480 Query: 481 QHPQAAQLHAAAQWIAEQTGAKLGVLTEGGNAVGGYLANAVPAAGKAAAVFAQPRKAYLL 540 QHPQAAQLHAAAQWIAEQTGAKLGVLTEGGNAVGGYLANAVPAAGKAAAVFAQPRKAYLL Sbjct: 481 QHPQAAQLHAAAQWIAEQTGAKLGVLTEGGNAVGGYLANAVPAAGKAAAVFAQPRKAYLL 540 Query: 541 LNAEPELDSYDPQAASAALKQADMVVVMSAFKHGADYADVLLPIAPFTETSGTFVNAEGR 600 LNAEPELDSYDPQAASAALKQADMVVVMSAFKHGADYADVLLPIAPFTETSGTFVN EGR Sbjct: 541 LNAEPELDSYDPQAASAALKQADMVVVMSAFKHGADYADVLLPIAPFTETSGTFVNTEGR 600 Query: 601 AQSFNGTVKPAGDARPGWKVLRVLGNLLELENFEYETSESIRDELLGKETDLSARLNNLA 660 AQSFNGTVKPAGDARPGWKVLRVLGNLLELENFEYETSESIRDELLGKETDLSARLNNLA Sbjct: 601 AQSFNGTVKPAGDARPGWKVLRVLGNLLELENFEYETSESIRDELLGKETDLSARLNNLA 660 Query: 661 SVQLQAVSTAAAEVERVADVPIYFADPIARRSAPLLETRDGQAPKAWLSAALASKLGVAE 720 SVQL+AVSTAAAEVERVADVPIYFADPIARRSAPLLETRDGQAP+AWLSAALASKLGVAE Sbjct: 661 SVQLRAVSTAAAEVERVADVPIYFADPIARRSAPLLETRDGQAPRAWLSAALASKLGVAE 720 Query: 721 GDQVKVKQGSGSAALTAAIDKKLPENVVRVAAAHASTAALGGMFGAIVVEKA 772 GDQVKVKQGSGSAALTAAIDKKLPENVVRVAAAHASTAALGGMFGAIVVEKA Sbjct: 721 GDQVKVKQGSGSAALTAAIDKKLPENVVRVAAAHASTAALGGMFGAIVVEKA 772 >gi|300310808|ref|YP_003774900.1| NAD-dependent formate dehydrogenase subunit alpha [Herbaspirillum seropedicae SmR1] Length = 957 Score = 220 bits (560), Expect = 3e-58, Method: Compositional matrix adjust. Identities = 233/882 (26%), Positives = 370/882 (41%), Gaps = 148/882 (16%) Query: 1 MVEIEIDGKKVEVQEGSMVMDAANKLGTYIPHFCYHKKLSIAANCRMCLVEVEKAPKPL- 59 MV +EIDG V V G+ VM A+ + G +P C L +CR+CLVE+EK + + Sbjct: 19 MVTLEIDGVAVTVPAGTSVMRASVEAGINVPKLCATDSLEPFGSCRLCLVEIEKDGRKMK 78 Query: 60 --PA-CATPVNAGMIVRTASDKAVKAQKGVMEFLLINHPLDCPICDQGGECQLQDLA--V 114 PA C TP GM V+T + K + ++GVME + +HPLDC C G C+LQD+A V Sbjct: 79 GYPASCTTPCEPGMKVQTQTPKLAEIRRGVMELYISDHPLDCLTCPTNGNCELQDMAGVV 138 Query: 115 GYGGSSSRYQEEKRVVEPKDVGPLISMKEMNRCIHCTRCVRFGQEVAGVMELGMLGRGEH 174 G Y+ E KD + ++CI C RCVR +E G L + GRG Sbjct: 139 GLREVRYGYEGENHTKMKKDESNPYFTYDPSKCIVCNRCVRACEETQGTFALTINGRGFE 198 Query: 175 SEITTF-VGKTVDSEL--SGNMIDLCPVGALTSKPFRYSARTWELSRRKSVSPHDSVGAN 231 S ++ + ++SE G ++ CP LT K + E S+ + + + VG + Sbjct: 199 SRVSAGQMEAFMESECVSCGACVEACPTATLTEKTVIMLGQA-EHSKVTTCA-YCGVGCS 256 Query: 232 LIVQVKNGKVMRVLPFENEEVNECWISDKDRFAYEALDSEQRLTKPMLKQ--DGKWQEVD 289 ++K +V+R++P+++ + N+ K RFA+ + R+ PM+++ + W+EV Sbjct: 257 FKAEMKGNEVVRMVPYKDGKANQGHSCVKGRFAWGYATHKDRILNPMIRKSINDPWREVS 316 Query: 290 WQTALEYVAHGLRNIRHEHGADAIAALGTPYSTLEELSLLQKIVR-GVGSDNVDFRLR-- 346 W+ AL Y A R I+ +HG D+I + + T EE L+QK+VR G++NVD R Sbjct: 317 WEEALSYAASEFRRIQAKHGKDSIGGITSSRCTNEETYLVQKLVRAAFGNNNVDTCARVC 376 Query: 347 --QSDFGLDGQIKPWLGMSIVE-FSQLNRVFVIGSFLRKDHPLLSARLRQSAKSGAKVSV 403 + +GL + G E + + + ++G+ HP+ ++++++ + GAK+ V Sbjct: 377 HSPTGYGLKQTLGESAGTQTFESVMKSDVILIMGANPAAGHPVFASQIKRRVRQGAKLIV 436 Query: 404 LHATDDDLLMPVANKIIAAP---------------SAWLSALAEVAVA--------VAEA 440 + P +++ +P +A ++ALA V ++ + E Sbjct: 437 ID--------PRTTEMVKSPHIQADYHLKLRPGTNTAIITALAHVILSEGLQDEAYIQER 488 Query: 441 KGVA--------------APSAYAGVTASDAAKATAASLL--SGDAKGVFLGNAAVQHPQ 484 + +P A A +T A A+ L +G ++ G +H Q Sbjct: 489 CDLQSYQDWKEFVLREDNSPEAMAAITGVPAEIIRGAARLYATGGNAAIYYGLGVTEHAQ 548 Query: 485 AAQLHAAAQWIAEQTG--AKLGV----LTEGGNAVG----GYLANAVPAAGKA--AAVFA 532 + +A TG + GV L N G G + +P + V Sbjct: 549 GSTTVMGIANLAMCTGNVGREGVGVNPLRGQNNVQGSCDMGSFPHELPGYRHISDSTVRN 608 Query: 533 QPRKAY-LLLNAEPELD------------------------SYDP--QAASAALKQADMV 565 Q +A+ + LN EP L DP Q +AAL+ + + Sbjct: 609 QFEQAWGVTLNPEPGLRIPNMFDAALDGSFKGLYCEGEDIVQSDPNTQHVAAALQAMECI 668 Query: 566 VVMSAF-KHGADYADVLLPIAPFTETSGTFVNAEGRAQSFNGTVKPAGDARPGWKVLRVL 624 VV F A YA V LP + F E GTF NAE R V P + W+V L Sbjct: 669 VVQDLFLNETAKYAHVFLPGSSFLEKDGTFTNAERRISRVR-KVMPPKAGKSDWEVTVAL 727 Query: 625 GNLLELENFEYETSESIRDELLGKETDLSA----RLNNLASVQLQAVSTAAAEVERVADV 680 L Y I DE+ +L L S+Q + +A E + V Sbjct: 728 AEALGYP-MPYNHPSEIMDEIAALTPSFHGVSYDKLEKLGSIQW-PCNESAPEGTPIMHV 785 Query: 681 ------------PIYFA-DPIARRSAPLL--------------ETRDGQAPKAWLSAAL- 712 YFA D + PL+ +TR + + W S Sbjct: 786 GSFIRGKGKFINTQYFATDEKVTQRYPLILTTGRILSQYNVGAQTRRTENSQ-WHSEDRL 844 Query: 713 ------ASKLGVAEGDQVKVKQGSGSAALTAAIDKKLPENVV 748 A G+ EGD V V+ +G L A + +K+ VV Sbjct: 845 EIHPHDAEDRGIREGDWVGVESRAGQTVLRATVTEKVQPGVV 886 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.131 0.377 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,018,475 Number of extensions: 78039 Number of successful extensions: 309 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 304 Number of HSP's successfully gapped: 2 Length of query: 772 Length of database: 1,619,870 Length adjustment: 93 Effective length of query: 679 Effective length of database: 1,179,515 Effective search space: 800890685 Effective search space used: 800890685 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 134 (56.2 bits)