BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0029.0302.p (308 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311076|ref|YP_003775168.1| sugar kinase,ribokinase family ... 615 e-177 gi|300310831|ref|YP_003774923.1| 2-ketogluconate kinase [Herbasp... 114 9e-27 gi|300310353|ref|YP_003774445.1| ribokinase [Herbaspirillum sero... 99 5e-22 gi|300311612|ref|YP_003775704.1| sugar kinase [Herbaspirillum se... 67 2e-12 gi|300311742|ref|YP_003775834.1| transferase kinase [Herbaspiril... 67 2e-12 >gi|300311076|ref|YP_003775168.1| sugar kinase,ribokinase family protein [Herbaspirillum seropedicae SmR1] Length = 308 Score = 615 bits (1587), Expect = e-177, Method: Compositional matrix adjust. Identities = 304/307 (99%), Positives = 306/307 (99%) Query: 1 MSKTILAYGEAMVEFNQRIDAPRQFLQGYGGDTSNFAIAAARQGAASAYLSAVGDDHFGQ 60 MSKTILAYGEAMVEFNQRIDAPRQFLQGYGGDTSNFAIAAARQGAASAYLSAVGDDHFGQ Sbjct: 1 MSKTILAYGEAMVEFNQRIDAPRQFLQGYGGDTSNFAIAAARQGAASAYLSAVGDDHFGQ 60 Query: 61 DLLALWQREEVDVRHVAIAPGGSTGIYFVTHDAAGHHFHYRRAGSAASAFQTKALPLEAI 120 DLLALWQREEVDVRHVAIAPGGSTGIYFVTHDAAGHHFHYRRAGSAASA+Q KALPLEAI Sbjct: 61 DLLALWQREEVDVRHVAIAPGGSTGIYFVTHDAAGHHFHYRRAGSAASAYQAKALPLEAI 120 Query: 121 AAAGALHLSGISLAISESACEAGLAAMAHARASGVTTSLDTNLRLRLWPLERARARMEEA 180 AAAGALHLSGISLAISESACEAGLAAMAHARASGVTTSLDTNLRLRLWPLERARARMEEA Sbjct: 121 AAAGALHLSGISLAISESACEAGLAAMAHARASGVTTSLDTNLRLRLWPLERARARMEEA 180 Query: 181 FRLCDICLPSWEDVSLLTGLEDRDAIVDRLLSYGVKLVAFKLGKEGCYVATSDQRRMVAP 240 FRLCDICLPSWEDVSLLTGLEDRDAIVDRLLSYGVKLVAFKLGKEGCYVATSDQRRMVAP Sbjct: 181 FRLCDICLPSWEDVSLLTGLEDRDAIVDRLLSYGVKLVAFKLGKEGCYVATSDQRRMVAP 240 Query: 241 YPVDALDATGAGDCFGGAFMAELMAGRDAFAAARYANVAAALSTTGYGAVEPIPRRAQVE 300 YPVD+LDATGAGDCFGGAFMAELMAGRDAFAAARYANVAAALSTTGYGAVEPIPRRAQVE Sbjct: 241 YPVDSLDATGAGDCFGGAFMAELMAGRDAFAAARYANVAAALSTTGYGAVEPIPRRAQVE 300 Query: 301 AVLAKTS 307 AVLAKTS Sbjct: 301 AVLAKTS 307 >gi|300310831|ref|YP_003774923.1| 2-ketogluconate kinase [Herbaspirillum seropedicae SmR1] Length = 313 Score = 114 bits (286), Expect = 9e-27, Method: Compositional matrix adjust. Identities = 97/309 (31%), Positives = 131/309 (42%), Gaps = 8/309 (2%) Query: 1 MSKTILAYGEAMVEFNQRIDAPRQFLQGY----GGDTSNFAIAAARQGAASAYLSAVGDD 56 M ++ +GEA+ P + ++ Y G +N AI AR G + S VG+D Sbjct: 3 MQLDVVTWGEALALLVADEVGPFEEVEKYTRRLAGAETNVAIGLARLGLKVGWASRVGND 62 Query: 57 HFGQDLLALWQREEVDVRHVAIAPGGSTGIYFVTHDAAGHH--FHYRRAGSAASAFQTKA 114 FG+ + +E V+V V T I G Y R GSAAS Sbjct: 63 AFGRFIRQRVAQEGVEVSRVITDMEFRTAIQLKAKAVGGADPAIEYYRKGSAASHLSVDD 122 Query: 115 LPLEAIAAAGALHLSGISLAISESACEAGLAAMAHARASGVTTSLDTNLRLRLWPLERAR 174 AA LH +GI+ A+S + AM R G T S D NLR LWP + Sbjct: 123 FDAGYFGAARHLHATGIAPALSATTMAFAHQAMDFMRGQGKTISFDPNLRPMLWPSQEVM 182 Query: 175 ARMEEAFRL-CDICLPSWEDVSLLTGLEDRDAIVDRLLSYGVKLVAFKLGKEGCYVATSD 233 A+ A D LP + +LTG +D I L GVKLV KLG EG Y Sbjct: 183 AQQLNALAFKADWVLPGLSEGKILTGHDDAREIAGFYLERGVKLVVIKLGAEGAYWRNGQ 242 Query: 234 QRRMVAPYPV-DALDATGAGDCFGGAFMAELMAGRDAFAAARYANVAAALSTTGYGAVEP 292 VA PV + +D GAGD F ++ ++ G A N A + G +E Sbjct: 243 GEGRVAGVPVKEVVDTVGAGDGFAVGVISGMLEGLPVPQAVMRGNRIGAFAIQVVGDMEG 302 Query: 293 IPRRAQVEA 301 +P RA++EA Sbjct: 303 LPTRAELEA 311 >gi|300310353|ref|YP_003774445.1| ribokinase [Herbaspirillum seropedicae SmR1] Length = 319 Score = 98.6 bits (244), Expect = 5e-22, Method: Compositional matrix adjust. Identities = 97/286 (33%), Positives = 134/286 (46%), Gaps = 22/286 (7%) Query: 25 FLQGYGGDTSNFAIAAARQGAASAYLSAVGDDHFGQDLLALWQREEVDVRHVAIAPGGST 84 F G GG SN A+AAAR GA ++SA+G D FG L+LW+RE + HV + G T Sbjct: 39 FAMGPGGKGSNQAVAAARVGAPVRFISAIGKDAFGDFALSLWRRETI-APHVRVIDGAPT 97 Query: 85 GIYFV-THDAAGHHFHYRRAGSAASAFQTKALPLEAIAAAGALHLSGISLAISESACEAG 143 G F+ +DA+G + G AAS + + E A+ + + + E EA Sbjct: 98 GAAFIYVNDASGDNAIIVVPG-AASQLSEQDVERE----RQAIEAAKVFVTQLEQPPEAA 152 Query: 144 LAAMAHARASGVTTSLDTNLRLRLWPLERARARMEEAFRLCDICLPSWEDVSLLTGLE-- 201 LA + ARA+G TT + L A + + LCD P+ + +LLTG+E Sbjct: 153 LAGLQIARAAGTTTVFNPAPAL---------AFPAQVYGLCDFITPNEHEAALLTGIEIH 203 Query: 202 ---DRDAIVDRLLSYGVKLVAFKLGKEGCYVATSDQR-RMVAPYPVDALDATGAGDCFGG 257 D D LL GV LG +G + + Q + A ++ GAGD F G Sbjct: 204 HIDDARRAADVLLERGVGCALITLGAQGSLLHSRSQSLHLPALCAGTVVETAGAGDAFNG 263 Query: 258 AFMAELMAGRDAFAAARYANVAAALSTTGYGAVEPIPRRAQVEAVL 303 AF A L G A AAR A AA+S T G +P R + A+L Sbjct: 264 AFAAGLAEGMSAEEAARLATAVAAISVTRPGTAPSMPSRQEALALL 309 >gi|300311612|ref|YP_003775704.1| sugar kinase [Herbaspirillum seropedicae SmR1] Length = 299 Score = 67.0 bits (162), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 85/283 (30%), Positives = 125/283 (44%), Gaps = 22/283 (7%) Query: 24 QFLQGYGGDTSNFAIAAAR---QGAASAYLSAVGDDHFGQDLLALWQREEVDVRHVAIAP 80 +F+ GG +N A+A AR G A ++ VGDD FG + +D R++ Sbjct: 30 RFMTIPGGKGANQAVACARLAAPGTRVAMVACVGDDAFGGQMRQSITACGIDDRYIDEVA 89 Query: 81 GGSTGIYFVTHDAAGHHFHYRRAGSAASAFQTKALPLEAIAAAGAL-HLSGISLAISESA 139 G +TGI + DA + AG+ L +E I A AL + I L E Sbjct: 90 GEATGIASIMVDANAQNSIVIAAGANGR------LDVERIERARALIEQASIVLLQLEVP 143 Query: 140 CEAGLAAMAHARASGVTTSLDTNLRLRLWPLERARARMEEAFRLCDICLPSWEDVSLLT- 198 + ++ A A G T L+ A+A + D + + + ++L Sbjct: 144 MATVIHSIELAHALGKTVVLNP---------APAQALPRALLQKIDYLILNEIEAAMLAE 194 Query: 199 -GLEDRDAIVDRLLSYGVKLVAFKLGKEGCYVATSD-QRRMVAPYPVDALDATGAGDCFG 256 ED + +L G + V LG++G Y + +D Q+R + V A+D T AGD F Sbjct: 195 EQSEDIPMLARKLHDLGARNVVVTLGEKGVYGSFADGQQRHLPARKVQAVDTTAAGDTFI 254 Query: 257 GAFMAELMAGRDAFAAARYANVAAALSTTGYGAVEPIPRRAQV 299 G F+ + GRD F A YA AAALS T GA IP R +V Sbjct: 255 GGFIGAIAQGRDQFEAIAYAQAAAALSVTRVGAQTSIPTRDEV 297 >gi|300311742|ref|YP_003775834.1| transferase kinase [Herbaspirillum seropedicae SmR1] Length = 657 Score = 66.6 bits (161), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 75/304 (24%), Positives = 119/304 (39%), Gaps = 25/304 (8%) Query: 15 FNQRIDAP----RQFLQGYGGDTSNFAIAAARQGAASAYLSAVGDDHFGQDLLALWQREE 70 + Q++ AP F + GG ++N A AR G SA L+ +GD+ GQ L A E Sbjct: 34 YAQQVGAPLSDVSSFAKYLGGSSANIAFGCARLGLKSAMLARIGDEANGQFLKAELAAEG 93 Query: 71 VDVRHVAIAPGGSTGIYFV-THDAAGHHFHYRRAGSAASAFQTKALPLEAIAAAGALHLS 129 DV HV + P T + + D + R A A + + IA++ AL ++ Sbjct: 94 CDVSHVTVDPQRLTALVMLGLKDKDSFPLIFYRDNCADMALAPEDIDEAFIASSKALLIT 153 Query: 130 GISLAISESACEAGLAAMAHARASGVTTSLDTNLRLRLWPL-------------ERARAR 176 G + + + L A+ +AR V T LD + R LW L + Sbjct: 154 GTHFSTPQVHRSSSL-ALQYARRHNVRTVLDIDYRPVLWGLTSKGDGETRFIASDGVTQH 212 Query: 177 MEEAFRLCDICLPSWEDVSLLTGLEDRDAIVDRLLSYGVKLVAFKLGKEGCYVATS---- 232 ++ D+ + + E+ + G D A + + + + K G GC V Sbjct: 213 LQGILPQFDLVVGTEEEFMIAGGSTDIMAALRAVRALCAATLVVKRGPLGCVVLHGAIPE 272 Query: 233 --DQRRMVAPYPVDALDATGAGDCFGGAFMAELMAGRDAFAAARYANVAAALSTTGYGAV 290 D V+ L+ GAGD F F+ + D RYAN AL + + Sbjct: 273 RLDDGFHYRGVQVEVLNVLGAGDAFISGFLKGWLNEEDDEHCCRYANACGALVVSRHACA 332 Query: 291 EPIP 294 +P Sbjct: 333 PAMP 336 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.133 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 776,490 Number of extensions: 24773 Number of successful extensions: 103 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 96 Number of HSP's successfully gapped: 6 Length of query: 308 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 223 Effective length of database: 1,217,395 Effective search space: 271479085 Effective search space used: 271479085 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)