BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0029.0274.p (266 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313109|ref|YP_003777201.1| short-chain alcohol dehydrogena... 541 e-155 gi|300311568|ref|YP_003775660.1| toluenesulfonate Zinc-independe... 133 1e-32 gi|300311900|ref|YP_003775992.1| short chain dehydrogenase [Herb... 128 6e-31 gi|300313172|ref|YP_003777264.1| short-chain alcohol dehydrogena... 126 2e-30 gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum s... 124 1e-29 >gi|300313109|ref|YP_003777201.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 266 Score = 541 bits (1393), Expect = e-155, Method: Compositional matrix adjust. Identities = 262/266 (98%), Positives = 265/266 (99%) Query: 1 MDKLMQGKAIIVTGAGSVGEGWGNGKAAAVLYAREGGQVLAVDRSIEAALQTQDIIRSEG 60 MDKLMQGKAIIVTGAGSVGEGWGNGKAAAVLYAREGG+VLAVDRSIEAALQTQDIIRSEG Sbjct: 1 MDKLMQGKAIIVTGAGSVGEGWGNGKAAAVLYAREGGRVLAVDRSIEAALQTQDIIRSEG 60 Query: 61 GVCEVLAADVSRSAEVKAIAEAAMDHFGRVDVLHNNVGIADVGGPVETSEESWNRLVAVN 120 GVCEVLAADVSR+ EVKAIAEAAMDHFGRVDVLHNNVGIADVGGPVETSEESWNRLVAVN Sbjct: 61 GVCEVLAADVSRNTEVKAIAEAAMDHFGRVDVLHNNVGIADVGGPVETSEESWNRLVAVN 120 Query: 121 QTSLFLTHKHVLPIMEKQKKGAIVNISSLAALRWIGFPYLGYTATKAAILAFTKNVAMQY 180 QTSLFLTHKHVLPIMEKQ+KGAIVNISSLAALRWIGFPYLGYTATKAAILAFTKNVAMQY Sbjct: 121 QTSLFLTHKHVLPIMEKQRKGAIVNISSLAALRWIGFPYLGYTATKAAILAFTKNVAMQY 180 Query: 181 APMGIRANCVLPGMMDTPMIREPLKASYGGDIEQMRAKRNAQCPMGFMGDAWDVAHASLF 240 APMGIRANCVLPGMMDTPMIREPLKASYGGDIEQMRAKRNAQCPMGFMGDAWDVAHASLF Sbjct: 181 APMGIRANCVLPGMMDTPMIREPLKASYGGDIEQMRAKRNAQCPMGFMGDAWDVAHASLF 240 Query: 241 LASDHARYITGLDMIVDGGLSLRCAG 266 LASDHARYITGLDMIVDGGLSLRCAG Sbjct: 241 LASDHARYITGLDMIVDGGLSLRCAG 266 >gi|300311568|ref|YP_003775660.1| toluenesulfonate Zinc-independent alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 252 Score = 133 bits (335), Expect = 1e-32, Method: Compositional matrix adjust. Identities = 81/237 (34%), Positives = 128/237 (54%), Gaps = 14/237 (5%) Query: 32 YAREGGQVLAVDRSIEAALQTQDIIRSEGGVCEVLAADVSRSAEVKAIAEAAMDHFGRVD 91 YAREG ++ D L+ + I++ GG ADVS+ A++ + A++HFG++D Sbjct: 25 YAREGAAIVVADIGEAGGLRVVEEIKAAGGRAVFAKADVSKRADMDQLLATALEHFGKLD 84 Query: 92 VLHNNVGIADVGGP-VETSEESWNRLVAVNQTSLFLTHKHVLPIMEKQKKGAIVNISSLA 150 ++ NN G P +E E+ ++R+ AVN S+FL+ K +P + GA +NI+S A Sbjct: 85 IVVNNAGTTHRNRPMLEVEEDEFDRVYAVNVKSIFLSAKTFVPYFRQVGGGAFINIASTA 144 Query: 151 ALRWIGFPYLG---YTATKAAILAFTKNVAMQYAPMGIRANCVLPGMMDTPMIREPLKAS 207 +R P G Y +K A++ +K++A + P IR NCV P + T ++ E + Sbjct: 145 GIR----PRPGLTWYNGSKGAVITTSKSMAAELGPDKIRVNCVNPVISATGLLSEFMGVP 200 Query: 208 YGGDIEQMRAKRNAQCPMGFMGDAWDVAHASLFLASDHARYITGLDMIVDGGLSLRC 264 D + R K A PMG D+A+A L+L SD A ++TG+ + VDGG RC Sbjct: 201 ---DTPENRKKFVATIPMGRFSTPEDIANACLYLGSDEAEFVTGVCIEVDGG---RC 251 >gi|300311900|ref|YP_003775992.1| short chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 128 bits (321), Expect = 6e-31, Method: Compositional matrix adjust. Identities = 91/259 (35%), Positives = 132/259 (50%), Gaps = 18/259 (6%) Query: 5 MQGKAIIVTGAGSVGEGWGNGKAAAVLYAREGGQVLAVDRSIEAALQTQDIIRSEGGVCE 64 +QGK ++TGA + G G+A A+L+AR G ++ R +A Q +I ++GG Sbjct: 4 LQGKVALITGASA-----GIGRATALLFARHGAALILTARRQDALEQVAALICAQGGRAS 58 Query: 65 VLAADVSRSAEVKAIAEAAMDHFGRVDVLHNNVGIADVGGPV-ETSEESWNRLVAVNQTS 123 V+A DV +A + A+D FG +D+ NN G P+ E S + W + N TS Sbjct: 59 VVAGDVGEAATHERGVAMALDEFGGLDIAINNAGAVGACKPLAEISPQQWQDTLHANLTS 118 Query: 124 LFLTHKHVLPIMEKQKKGAIVNISSLAALRWIGFPYL-GYTATKAAILAFTKNVAMQYAP 182 FL + +P M + GAIV SS G P + Y A KA ++ K +A YA Sbjct: 119 AFLGARCQIPAMLARGAGAIVFTSSFVG-SSAGLPGMAAYGAAKAGLMGLVKGIAADYAV 177 Query: 183 MGIRANCVLPGMMDTPMIREPLKASYGGDIEQMRAKRNAQCPMGFMGDAWDVAHASLFLA 242 GIRAN +LPG +DT M +P + + + M KR AQ ++A A+LFLA Sbjct: 178 QGIRANALLPGGVDTDMGGDPQQKQWAAGLHAM--KRIAQPE--------EIAAAALFLA 227 Query: 243 SDHARYITGLDMIVDGGLS 261 S A ++TG + DGG S Sbjct: 228 SPMASFVTGTALYADGGNS 246 >gi|300313172|ref|YP_003777264.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 254 Score = 126 bits (316), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 87/260 (33%), Positives = 138/260 (53%), Gaps = 17/260 (6%) Query: 5 MQGKAIIVTGAGSVGEGWGNGKAAAVLYAREGGQVLAVDRSIEAALQTQDIIRSEGGVCE 64 + GK +++T A G G+A+ L+AREG +V+A D S D + GV E Sbjct: 8 LAGKTVLITAAAQ-----GIGRASTELFAREGARVIATDIS----KPHLDELAGIAGV-E 57 Query: 65 VLAADVSRSAEVKAIAEAAMDHFGRVDVLHNNVGIADVGGPVETSEESWNRLVAVNQTSL 124 DV+ A +KA+ + G +DVL N G G +E +++W+ +N ++ Sbjct: 58 THLLDVTDDAAIKAL----VAKIGTIDVLFNCAGYVAAGNILECDDKAWDFSFNLNAKAM 113 Query: 125 FLTHKHVLPIMEKQKKGAIVNISSLAALRWIGFPYLGYTATKAAILAFTKNVAMQYAPMG 184 F T + VLP M +K G+IVNI+S A+ Y A+KAA++ TK+VA + G Sbjct: 114 FHTIRAVLPGMLAKKAGSIVNIASAASSVKGVANRFAYGASKAAVVGLTKSVAADFVAQG 173 Query: 185 IRANCVLPGMMDTPMIREPLKA---SYGGDIEQMRAKRNAQCPMGFMGDAWDVAHASLFL 241 IR N + PG +++P + + + G E++RA + PMG +G A +VA +L+L Sbjct: 174 IRCNAICPGTIESPSLNQRISTQAKETGKSEEEVRAAFVGRQPMGRIGKAEEVAALALYL 233 Query: 242 ASDHARYITGLDMIVDGGLS 261 ASD + + TG ++DGG S Sbjct: 234 ASDESNFTTGSIHMIDGGWS 253 >gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 124 bits (310), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 81/260 (31%), Positives = 136/260 (52%), Gaps = 18/260 (6%) Query: 4 LMQGKAIIVTGAGSVGEGWGNGKAAAVLYAREGGQVLAVDRSIEAALQTQDIIRSEGGVC 63 L++ K ++TG + G G A A L A +G +V+ +D + I S+ Sbjct: 2 LLKDKVAVITGGAGIN---GLGYATARLMAEQGARVVVLDLAQADPAAAAARIGSQH--- 55 Query: 64 EVLAADVSRSAEVKAIAEAAMDHFGRVDVLHNNVGIADVGGPVETSEESWNRLVAVNQTS 123 L ADV+ A + A A + FGR+D+L NN GI +E + ++R++ V+ Sbjct: 56 LGLVADVTDKASCETAAAAILQQFGRIDILVNNAGITQPVKTLEITGADYDRILDVSLRG 115 Query: 124 LFLTHKHVLPIMEKQKKGAIVNISSLAALR---WIGFPYLGYTATKAAILAFTKNVAMQY 180 + VLP M Q+ G+I+ ISS++A R +G P+ Y+A KA +L + +A ++ Sbjct: 116 TLYMSQAVLPAMRAQQNGSIICISSVSAQRGGGILGGPH--YSAAKAGVLGLARAMAREF 173 Query: 181 APMGIRANCVLPGMMDTPMIREPLKASYGGDIEQMRAKRNAQCPMGFMGDAWDVAHASLF 240 P GIR N + PG++ T +I+ L E+ +A+ P+ +G A D+ A +F Sbjct: 174 GPEGIRVNSITPGLIGTDIIKGKLT-------EEKKAEIAETIPLARLGRADDIGGACVF 226 Query: 241 LASDHARYITGLDMIVDGGL 260 L SD ++Y TG+ + V+GG+ Sbjct: 227 LGSDLSQYCTGITLDVNGGM 246 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.134 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 848,261 Number of extensions: 34651 Number of successful extensions: 266 Number of sequences better than 1.0e-08: 45 Number of HSP's gapped: 181 Number of HSP's successfully gapped: 46 Length of query: 266 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 182 Effective length of database: 1,222,130 Effective search space: 222427660 Effective search space used: 222427660 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)