BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0029.0269.p (527 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313115|ref|YP_003777207.1| long-chain-fatty-acid-CoA ligas... 1090 0.0 gi|300313157|ref|YP_003777249.1| long-chain-fatty-acid-CoA ligas... 162 5e-41 gi|300309365|ref|YP_003773457.1| acetyl-coenzyme A synthetase [H... 154 1e-38 gi|300313986|ref|YP_003778078.1| acyl-CoA synthetase/AMP-acid li... 151 1e-37 gi|300312417|ref|YP_003776509.1| propionyl-CoA ligase [Herbaspir... 141 1e-34 >gi|300313115|ref|YP_003777207.1| long-chain-fatty-acid-CoA ligase [Herbaspirillum seropedicae SmR1] Length = 556 Score = 1090 bits (2818), Expect = 0.0, Method: Compositional matrix adjust. Identities = 525/527 (99%), Positives = 527/527 (100%) Query: 1 MNLGRLLSDVARRFPDEPGLILAQGAADGSDRLIPWREINARVDALAHALTRRGVKKGDK 60 MNLGRLLSDVARRFPDEPGLILAQGAADGSDRLIPWREINARVDALAHALTRRGVKKGDK Sbjct: 30 MNLGRLLSDVARRFPDEPGLILAQGAADGSDRLIPWREINARVDALAHALTRRGVKKGDK 89 Query: 61 MLVHSRNNQQIFESAWAAFKMGVVWVPTNFRITPPEAAYLGQSSGACVMLYDRGFAQYVD 120 MLVHSRNNQQIFESAWAAFKMGVVWVPTNFRITPPEAAYLGQSSGACVMLYDRGFAQYVD Sbjct: 90 MLVHSRNNQQIFESAWAAFKMGVVWVPTNFRITPPEAAYLGQSSGACVMLYDRGFAQYVD 149 Query: 121 AVKAASPALEHVVALADARAGELDYEALVREGSGAAFREAEVDYDDPLWFFYTSGTTGHP 180 AVKAASPALEHVVALADARAGELDYEALVREGSGAAFREAEVDYDDPLWFFYTSGTTGHP Sbjct: 150 AVKAASPALEHVVALADARAGELDYEALVREGSGAAFREAEVDYDDPLWFFYTSGTTGHP 209 Query: 181 KAGMLSHGQMAFVVTNHLADLMPGLTHQSRSLVVAPLSHGAGVHAVINTARGAASILLSS 240 KAGMLSHGQMAFVVTNHLADLMPGLTHQSRSLVVAPLSHGAGVHAVINTARGAASILLSS Sbjct: 210 KAGMLSHGQMAFVVTNHLADLMPGLTHQSRSLVVAPLSHGAGVHAVINTARGAASILLSS 269 Query: 241 ERLVVEEAWQLVEKYRVDNMFTVPTIVKILVEDESVDRYDHSSLKHVIYAGAPMYRADQV 300 ERLVVEEAWQLVEKYRVDN+FTVPTIVKILVEDESVDRYDHSSLKHVIYAGAPMYRADQV Sbjct: 270 ERLVVEEAWQLVEKYRVDNLFTVPTIVKILVEDESVDRYDHSSLKHVIYAGAPMYRADQV 329 Query: 301 YALKKLGKVLVQYYGLGEVTGNITFLPPYLHFEDDAHPQARVGTCGMPRTGMQIAILNDD 360 YALKKLGKVLVQYYGLGEVTGNITFLPPYLHFEDDAHPQARVGTCGMPRTGMQIAILNDD Sbjct: 330 YALKKLGKVLVQYYGLGEVTGNITFLPPYLHFEDDAHPQARVGTCGMPRTGMQIAILNDD 389 Query: 361 GQELAPFETGEICVRGPAVFMGYHNNPEANAKAFKHDWFHTGDLGHVDQDGFLYITGRSS 420 GQELAPFETGEICVRGPAVFMGYHNNPEANAKAFKHDWFHTGDLGHVDQDGFLYITGRSS Sbjct: 390 GQELAPFETGEICVRGPAVFMGYHNNPEANAKAFKHDWFHTGDLGHVDQDGFLYITGRSS 449 Query: 421 DMYISGGSNVYPREIEEALLTHPAVSEVAVLGVPDPKWGESGLAVIVCKAGQQVDGEALL 480 DMYISGGSNVYPREIEEALLTHPAVSEVAVLGVPDPKWGESGLAVIVCKAGQQVDGEALL Sbjct: 450 DMYISGGSNVYPREIEEALLTHPAVSEVAVLGVPDPKWGESGLAVIVCKAGQQVDGEALL 509 Query: 481 AHLETRIGKYKWPRRFIYWDSMPKSGYGKIVKKQIKALLEEQGEYRL 527 AHLE+RIGKYKWPRRFIYWDSMPKSGYGKIVKKQIKALLEEQGEYRL Sbjct: 510 AHLESRIGKYKWPRRFIYWDSMPKSGYGKIVKKQIKALLEEQGEYRL 556 >gi|300313157|ref|YP_003777249.1| long-chain-fatty-acid-CoA ligase [Herbaspirillum seropedicae SmR1] Length = 566 Score = 162 bits (411), Expect = 5e-41, Method: Compositional matrix adjust. Identities = 137/519 (26%), Positives = 229/519 (44%), Gaps = 45/519 (8%) Query: 32 RLIPWREINARVDALAHALTRRGVKKGDKMLVHSRNNQQIFESAWAAFKMGVVWVPTNFR 91 + + +RE+++ AL L G++KG ++ + N Q + A + G V N Sbjct: 47 KYLSYRELDSLSRALGGWLQGLGLQKGARVAIMMPNVLQYPIAIAAILRAGYTVVNVNPL 106 Query: 92 ITPPEAAYLGQSSGACVMLYDRGFAQYVDAVKAASPALEHVVA-----LADARAGEL--- 143 T E + + SGA + FA + V A + ++H+V L A G L Sbjct: 107 YTARELEHQLKDSGAEAIFVLENFACTLQKVVAHT-NIKHIVVASMGELLGALKGTLVNL 165 Query: 144 ------------------DYEALVREGSGAAFREAEVDYDDPLWFFYTSGTTGHPKAGML 185 + + + G + + +DD + YT GTTG K +L Sbjct: 166 VVRHVKKLVPAWSLPNAITFSQALSQARGKTLQPVTLTHDDIAFLQYTGGTTGVAKGAVL 225 Query: 186 SHGQMAFVVTNHLADLMPGL-----THQSRSLVVAPLSH--GAGVHAVINTARGAASILL 238 +H + V L P L Q + PL H V ++ T GA ++L+ Sbjct: 226 THRNLVANVLQAEEWLTPALKAGKPIDQLVFVCALPLYHIFALTVCNMLGTREGALNLLI 285 Query: 239 SSERLVVEEAWQLVEKYRVDNMFTVPTIVKILVEDESVDRYDHSSLKHVIYAGAPMYRAD 298 + R + +L KY+V+ V T+ L+ + R D S + + G + ++ Sbjct: 286 PNPRDIPGFVKELA-KYKVNTFPAVNTLYNALLNNADFARLDFSGYRCCVGGGMAVQKSV 344 Query: 299 QVYALKKLGKVLVQYYGLGEVTGNITFLPPYLHFEDDAHPQARVGTCGMPRTGMQIAILN 358 K G +++ YGL E + + P + GT G+P + AIL+ Sbjct: 345 ADKWKKLTGCPIIEGYGLSETSPIASANPCTI--------DEYTGTIGLPLPSTEFAILD 396 Query: 359 DDGQELAPFETGEICVRGPAVFMGYHNNPEANAKAFKHD-WFHTGDLGHVDQDGFLYITG 417 DDGQ + + GEI +RGP V GY P+ AK D +F TGD+G +D+ G+ I Sbjct: 397 DDGQPVPLGQPGEIAIRGPQVMPGYWQRPDETAKVMTPDGFFKTGDIGVMDERGYTKIVD 456 Query: 418 RSSDMYISGGSNVYPREIEEALLTHPAVSEVAVLGVPDPKWGESGLAVIVCKAGQQVDGE 477 R DM + G NVYP E+E + HP V E A +GVPD GE+ + + V + + + Sbjct: 457 RKKDMILVSGFNVYPNEVEGVVAQHPGVLECACVGVPDEHTGEA-VKLFVVRKDKTLSAA 515 Query: 478 ALLAHLETRIGKYKWPRRFIYWDSMPKSGYGKIVKKQIK 516 L+ + + + YK P+ + D +PK+ GKI++++++ Sbjct: 516 QLMDYCKEQFTGYKKPKYIEFRDELPKTNVGKILRRELR 554 >gi|300309365|ref|YP_003773457.1| acetyl-coenzyme A synthetase [Herbaspirillum seropedicae SmR1] Length = 549 Score = 154 bits (390), Expect = 1e-38, Method: Compositional matrix adjust. Identities = 141/510 (27%), Positives = 227/510 (44%), Gaps = 36/510 (7%) Query: 27 ADGSDRLIPWREINARVDALAHALTRRGVKKGDKMLVHSRNNQQIFESAWAAFKMGVVWV 86 ADGS + ++ D A+AL GV +GD++ ++ + + AA+K+G + V Sbjct: 56 ADGSVTSYSYDQLKILSDRFANALRAAGVGRGDRIGIYLSQRIETVITHLAAYKLGAITV 115 Query: 87 PTNFRITPPEAAYLGQSSGACVMLYDRGFAQYVDAVKAASPALEHVVALADARAGELDYE 146 P + P AY +SGA ++ D + + PAL V + + +L Sbjct: 116 PLFYLFGPDAIAYRLDNSGAVALVTDASGMEKA-TLAGELPALRLVFCV---ESSDLVLP 171 Query: 147 ALVREGSGAAFREAEVD-----YDDPLWFFYTSGTTGHPKAGMLSH----GQMAFVVTNH 197 AE+D DDP YTSGTTG K + +H G + V +H Sbjct: 172 QTTDFWDRLHAAPAELDPVLTLADDPAMIIYTSGTTGKAKGALHAHRVLLGHLPGVEVSH 231 Query: 198 LADLMPGLTHQSRSLVVAPLSHGAGVHAVI--NTARGAASILLSSERLVVEEAWQLVEKY 255 D P R A + G+ V+ + G A + E+ E + L+ ++ Sbjct: 232 --DSFP--QEGDRFWTPADWAWIGGLLDVLLPSLYHGVAVVARRLEKFDAAEVFGLLARH 287 Query: 256 RVDNMFTVPTIVKILVEDESVDRYDHSSLKHVIYAGAPMYRADQVYALKKLGKVLVQYYG 315 ++ N+F PT +K+L +V SL+ V G + + + LG + ++YG Sbjct: 288 QIRNVFFPPTALKMLRGAATVRAQADFSLRSVASGGETLGDDLIAWGREALGVTINEFYG 347 Query: 316 LGEVTGNITFLPPYLHFEDDAHPQARV-GTCGMPRTGMQIAILNDDGQELAPFETGEICV 374 E L +H V G+ G G + I+++ GQ L G I + Sbjct: 348 QTECN---------LVVSSSSHCYPSVSGSMGRAVPGHVVQIVDEQGQVLPHGTVGNIAI 398 Query: 375 RGP--AVFMGYHNNPEANAKAFKHDWFHTGDLGHVDQDGFLYITGRSSDMYISGGSNVYP 432 R P +F+ Y N EA + F D+ TGDLG +D+ G++ GR+ D+ S G + P Sbjct: 399 RAPDPVMFLRYWRNEEATREKFAGDFLLTGDLGSMDEQGYIRYLGRNDDVITSAGYRIGP 458 Query: 433 REIEEALLTHPAVSEVAVLGVPDPKWGESGLAVIVCKAGQQVDGEALLA----HLETRIG 488 IEE L+ HPAV AV+GV D E A +V K G D EAL A H+ ++ Sbjct: 459 AAIEECLMRHPAVRIAAVVGVKDALRTEVVKAFVVLKDGVTPD-EALKAELQQHVRAQLA 517 Query: 489 KYKWPRRFIYWDSMPKSGYGKIVKKQIKAL 518 +++PR + ++P + GKI++K +K + Sbjct: 518 AHEYPRLISFVAALPTTATGKIMRKTLKEM 547 >gi|300313986|ref|YP_003778078.1| acyl-CoA synthetase/AMP-acid ligase II protein [Herbaspirillum seropedicae SmR1] Length = 546 Score = 151 bits (381), Expect = 1e-37, Method: Compositional matrix adjust. Identities = 142/504 (28%), Positives = 216/504 (42%), Gaps = 29/504 (5%) Query: 36 WREINARVDALAHALTRRGVKKGDKMLVHSRNNQQIFESAWAAFKMGVVWVPTNFRITPP 95 W + R LA AL GV K D + N + E+ + G V N R+ Sbjct: 46 WDQTYRRTRRLASALQGLGVGKNDTVSAMLPNTPAMVEAHFGVPMAGAVLNALNIRLDAE 105 Query: 96 EAAYLGQSSGACVMLYDRGFAQYVDAVKAASPALEHVVA--------LADARAGELDYEA 147 ++ + A V+L D FA ++ PAL+ V +A R G L+YEA Sbjct: 106 SIVFMLRHGEAKVLLIDSEFAALAQQLRTQLPALKIVEVFDELGPPPVAGERFGHLEYEA 165 Query: 148 LVREGSGAAFREAEVDYDDPLWFFYTSGTTGHPKAGMLSHGQMAFVVTNHLADLMPGLTH 207 L+ G + D D + YTSGTTG PK + H A +++ + L Sbjct: 166 LLAGGDEHFDWQMPADEWDAIALNYTSGTTGDPKGVVYHHRGAALNAVSNILEW--DLPK 223 Query: 208 QSRSLVVAPLSHGAG-VHAVINTARGAASILLSSERLVVEEAWQLVEKYRVDNMFTVPTI 266 L P+ H G AR ++ L + + + L+ ++ + + P + Sbjct: 224 HPVYLWTLPMFHCNGWCFPWTVAARAGVNVCL--RKFEPKLVFDLIAEHGITHYCAAPIV 281 Query: 267 VKILVEDESVDRYDHSSLKHVIYAGAPMYRADQVYALKKLGKVLVQYYGLGEVTGNITFL 326 L R + AGAP A + ++ + L YGL EV G Sbjct: 282 HAALANAPEGWRAGIRGPVKAMVAGAPP-PAAVLAKMEAMQFELTHVYGLTEVYGPAAVC 340 Query: 327 PPY-----LHFEDDAHPQARVGTCGMPRTGMQIAILNDDGQELAPF-----ETGEICVRG 376 L + A ++R G ++G + +L+ D +AP E GEI RG Sbjct: 341 AEQDEWNTLSVDQRAVQKSRQGVRYHLQSG--VTVLSPD--TMAPVAADGEEIGEIMFRG 396 Query: 377 PAVFMGYHNNPEANAKAFKHDWFHTGDLGHVDQDGFLYITGRSSDMYISGGSNVYPREIE 436 GY N A +AF WFHTGDLG ++ DG++ I RS D+ ISGG N+ E+E Sbjct: 397 NICMKGYLKNDRATHEAFAGGWFHTGDLGVMNADGYIKIKDRSKDIIISGGENISSVEVE 456 Query: 437 EALLTHPAVSEVAVLGVPDPKWGESGLAVIVCKAGQQVDGEALLAHLETRIGKYKWPRRF 496 + L HPAV AV+ PD KWGE+ A + K G +V L + + +K P+ Sbjct: 457 DVLYRHPAVLAAAVVAQPDEKWGETPCAFVELKEGAEVGAAELTEFCRSHLAGFKVPKA- 515 Query: 497 IYWDSMPKSGYGKIVKKQIKALLE 520 IY+ +PK+ GKI K +++ ++ Sbjct: 516 IYFGPLPKTSTGKIQKFELRKRMK 539 >gi|300312417|ref|YP_003776509.1| propionyl-CoA ligase [Herbaspirillum seropedicae SmR1] Length = 628 Score = 141 bits (355), Expect = 1e-34, Method: Compositional matrix adjust. Identities = 142/566 (25%), Positives = 248/566 (43%), Gaps = 75/566 (13%) Query: 10 VARRFPD--EPGLILAQGAADGSDRLIPWREINARVDALAHALTRRGVKKGDKMLVHSRN 67 V R P+ + ++A S+R+ + E+ V A+A ++ GV++GD++L++ Sbjct: 58 VDRHLPERAQQAALIAISTETNSERVYTYAELQQEVMAMAASMQALGVQRGDRVLIYMPM 117 Query: 68 NQQIFESAWAAFKMGVVWVPTNFRITPPEAAYLGQSSGACVMLYDRGFAQYVDAVKAASP 127 + + A ++G V + G +S + D + + + A S Sbjct: 118 IAEAVFAMLACARIGAVH----------SVVFGGFASNSLASRIDDAQPRLIVSADAGSR 167 Query: 128 A---------LEHVVALADARAGELDYEALVREGSGAAFREAEVDYD-DPL--------- 168 L+ +A+A R + LV G R A+ D D PL Sbjct: 168 GGKVIPYKGLLDEAIAMAQHRPQHV---LLVDRGLAPMARTAQRDVDYAPLRTQHLGQQV 224 Query: 169 -----------WFFYTSGTTGHPKAGMLSHGQMAFVVTNHLADLM---PGLTHQSRSLVV 214 + YTSGTTG PK G A + + + + PG T+ S + Sbjct: 225 PVTWLESNASSYILYTSGTTGKPKGVQRDVGGYAVALASSMQHIFCGQPGETYFCTSDIG 284 Query: 215 APLSHGAGVHAVINTARGAASILLSSERLVVEEA--WQLVEKYRVDNMFTVPTIVKILVE 272 + H V+ + G A++L + + W +VEKY+V MF+ PT +++L + Sbjct: 285 WVVGHSYIVYGPLIA--GMATVLYEGLPIRPDAGIWWSIVEKYKVTRMFSAPTAIRVLKK 342 Query: 273 D--ESVDRYDHSSLKHVIYAGAPMYRADQVYALKKLGKVLVQYYGLGEVTGNITFLPPYL 330 E ++RYD SSLK + AG P+ + L ++ Y E I + + Sbjct: 343 QPPECMERYDTSSLKALYLAGEPLDETTSSWISGALKVPVIDNYWQTESGWPIISIAKGI 402 Query: 331 HFEDDAHPQARVGTCGMPRTGMQIAILND-DGQELAPFETGEICVRGP-------AVFMG 382 D P R+G+ G+P G ++AIL++ G+ P + G + + GP V+ Sbjct: 403 ----DDKP-TRLGSPGVPMPGYRLAILDEATGEPCGPDQKGVVAIEGPLPPGCMQTVYGD 457 Query: 383 YHNNPEANAKAFKHDWFHTGDLGHVDQDGFLYITGRSSDMYISGGSNVYPREIEEALLTH 442 ++ + + T D G D+DG+ +I GR+ D+ G + REIEE++ +H Sbjct: 458 DERFVNTYWRSLPREVYSTFDWGIRDKDGYYFILGRTDDVINVAGHRLGTREIEESISSH 517 Query: 443 PAVSEVAVLGVPDPKWGESGLAVIVCKAG-------QQVDGEA-LLAHLETRIGKYKWPR 494 P VSEVAV+GV D G+ A + K Q+ EA ++A ++ +IG P Sbjct: 518 PNVSEVAVVGVEDKLKGQVAFAFAIPKQAYTIATPEQRKALEAEIMAVVDKQIGAVGRPA 577 Query: 495 RFIYWDSMPKSGYGKIVKKQIKALLE 520 R + +PK+ GK++++ I+++ E Sbjct: 578 RVFFVSGLPKTRSGKLLRRAIQSICE 603 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.136 0.415 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,798,885 Number of extensions: 79596 Number of successful extensions: 300 Number of sequences better than 1.0e-08: 12 Number of HSP's gapped: 261 Number of HSP's successfully gapped: 17 Length of query: 527 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 437 Effective length of database: 1,193,720 Effective search space: 521655640 Effective search space used: 521655640 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)