BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0029.0266.p (431 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313118|ref|YP_003777210.1| proton glutamate symport protei... 856 0.0 gi|300311930|ref|YP_003776022.1| C4-dicarboxylate transport tran... 245 4e-66 gi|300313823|ref|YP_003777915.1| C4-dicarboxylate transport tran... 209 3e-55 >gi|300313118|ref|YP_003777210.1| proton glutamate symport protein [Herbaspirillum seropedicae SmR1] Length = 431 Score = 856 bits (2211), Expect = 0.0, Method: Compositional matrix adjust. Identities = 429/431 (99%), Positives = 431/431 (100%) Query: 1 MKKRIPQAAYIFAAMLLGILVGYLIFSHNDKAQAKELAGYISIASDLFLRLIKMVIAPLV 60 MKKRIPQAAYIFAAMLLGILVGYLIFSHNDKAQAKELAGYISIASDLFLRLIKMVIAPLV Sbjct: 1 MKKRIPQAAYIFAAMLLGILVGYLIFSHNDKAQAKELAGYISIASDLFLRLIKMVIAPLV 60 Query: 61 FSTLVVGIAHMGDAKSVGRIFGKSLAWFFIASLVSLALGMIMANLLQPGAGVALPSPDAA 120 FSTLVVGIAHMGDAKSVGRIFGKSLAWFFIASLVSLALGMIMANLLQPGAGVALPSPDAA Sbjct: 61 FSTLVVGIAHMGDAKSVGRIFGKSLAWFFIASLVSLALGMIMANLLQPGAGVALPSPDAA 120 Query: 121 GAGLATNKFTVKEFFNHLVPKSIVEAMAQNEVLQVVVFSMFFGIALASLGERGKHLLAVI 180 GAGLAT+KFTVKEFFNHLVPKSIVEAMAQNEVLQVVVFSMFFGIALASLGERGKHLLAVI Sbjct: 121 GAGLATSKFTVKEFFNHLVPKSIVEAMAQNEVLQVVVFSMFFGIALASLGERGKHLLAVI 180 Query: 181 DDLSHTMLKITVYVMKFAPVAVFAAMAATVAVNGLEILVSFAVFMRDFYFSLVLLWVILI 240 DDLSHTMLKITVYVMKFAPVAVFAAMAATVAVNGLEILVSFAVFMRDFYFSLVLLWVILI Sbjct: 181 DDLSHTMLKITVYVMKFAPVAVFAAMAATVAVNGLEILVSFAVFMRDFYFSLVLLWVILI 240 Query: 241 AVGFIFLKKRIFHLLALIKEAFLLAFATASSEAAYPKLLDALDRFGVKRKISSFVMPMGY 300 AVGFIFLKKRIFHLLALIKEAFLLAFATASSEAAYPKLLDALDRFGVKRKISSFVMPMGY Sbjct: 241 AVGFIFLKKRIFHLLALIKEAFLLAFATASSEAAYPKLLDALDRFGVKRKISSFVMPMGY 300 Query: 301 SFNLDGSMIYCTFATLFIAQAYGIHMPLSTQITMMLVLMLTSKGIAGVPRASLVVIAATL 360 SFNLDGSMIYCTFATLFIAQAYGIHMP+STQITMMLVLMLTSKGIAGVPRASLVVIAATL Sbjct: 301 SFNLDGSMIYCTFATLFIAQAYGIHMPISTQITMMLVLMLTSKGIAGVPRASLVVIAATL 360 Query: 361 HQFNIPEAGLLVILGIDTFLDMGRSATNAVGNSIASAVVAKWEGGLMTEEEAARAEVEIE 420 HQFNIPEAGLLVILGIDTFLDMGRSATNAVGNSIASAVVAKWEGGLMTEEEAARAEVEIE Sbjct: 361 HQFNIPEAGLLVILGIDTFLDMGRSATNAVGNSIASAVVAKWEGGLMTEEEAARAEVEIE 420 Query: 421 QEAEAKLHTEA 431 QEAEAKLHTEA Sbjct: 421 QEAEAKLHTEA 431 >gi|300311930|ref|YP_003776022.1| C4-dicarboxylate transport transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 441 Score = 245 bits (626), Expect = 4e-66, Method: Compositional matrix adjust. Identities = 158/434 (36%), Positives = 249/434 (57%), Gaps = 25/434 (5%) Query: 11 IFAAMLLGILVGYLIFSHNDKAQAKELAGYISIASDLFLRLIKMVIAPLVFSTLVVGIAH 70 + A+++GIL+G+ + +A + G D F++LIKM+IAP++F T+V+GIA Sbjct: 16 VLVAIVIGILLGHF---YPSTGEAMKPLG------DGFVKLIKMIIAPVIFCTVVIGIAG 66 Query: 71 MGDAKSVGRIFGKSLAWFFIASLVSLALGMIMANLLQPGAGV-----ALPSPDAAGAGLA 125 M D K VG+ G +L +F + S V+L +G+++ N LQPG G+ +L + A Sbjct: 67 MEDMKKVGKTGGLALLYFEVVSTVALIIGLLLVNFLQPGVGMNVDPASLDTKSIAAYTAP 126 Query: 126 TNKFTVKEFFNHLVPKSIVEAMAQNEVLQVVVFSMFFGIALASLGERGKHLLAVIDDLSH 185 +V +F ++P S+V+A A+ +VLQV++ ++ FG AL G RG + I+ +SH Sbjct: 127 GKMGSVTDFVLGIIPTSMVDAFAKGDVLQVLLVAVLFGFALHKFGGRGTLVFDFIEKISH 186 Query: 186 TMLKITVYVMKFAPVAVFAAMAATVAVNGLEILVSFAVFMRDFYFSLVLLWVILIAVGFI 245 + + +MK AP+ F AM+ T+ G+ L S A M FY + +L +++ + Sbjct: 187 VLFSVVGAIMKVAPIGAFGAMSFTIGKYGVGSLFSLAKLMGTFYLTCLLFIFVVLGIITR 246 Query: 246 FLKKRIFHLLALIKEAFLLAFATASSEAAYPKLLDALDRFGVKRKISSFVMPMGYSFNLD 305 I+ + IKE L+ T+SSE+ P++L ++ G K+ + V+P GYSFNLD Sbjct: 247 LHGFSIWKFVKYIKEELLIVLGTSSSESVLPRMLAKMENAGAKKTVVGLVIPTGYSFNLD 306 Query: 306 GSMIYCTFATLFIAQAYGIHMPLSTQITMMLVLMLTSKGIAGVPRASLVVIAATLHQF-N 364 G+ IY T A +FIAQA M ++T++ VL+LTSKG AG+ + +V+AATL + Sbjct: 307 GTAIYLTMAAVFIAQATNTPMTFVQELTLLGVLLLTSKGAAGITGSGFIVLAATLSAVGH 366 Query: 365 IPEAGLLVILGIDTFLDMGRSATNAVGNSIASAVVAKWEGGLMTEE----------EAAR 414 +P AGL +ILGID F+ R+ TN +GN +A+ VVAKW G L +E E A+ Sbjct: 367 VPVAGLALILGIDRFMSEARALTNTIGNGVATLVVAKWSGELDSERLTKVLNNETVEDAQ 426 Query: 415 AEVEIEQEAEAKLH 428 A I +AK+H Sbjct: 427 APESILDRTDAKMH 440 >gi|300313823|ref|YP_003777915.1| C4-dicarboxylate transport transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 445 Score = 209 bits (532), Expect = 3e-55, Method: Compositional matrix adjust. Identities = 144/399 (36%), Positives = 227/399 (56%), Gaps = 23/399 (5%) Query: 46 DLFLRLIKMVIAPLVFSTLVVGIAHMGDAKSVGRIFGKSLAWFFIASLVSLALGMIMANL 105 D F++LIKM+I +VF +V GI D K VG + K++ +F + + ++L LG+++A + Sbjct: 39 DGFIKLIKMIIPVIVFCVVVQGICGASDLKKVGSVGVKAIIYFEVVTTIALLLGLVLALV 98 Query: 106 LQPGAGVAL-PSP-DAAG-AGLATNKFTVKE-----FFNHLVPKSIVEAMAQNEVLQVVV 157 +QPGAG+ + PS DA+ +G N VKE F L+P + V A +VLQV++ Sbjct: 99 VQPGAGMNIDPSNLDASSLSGYMANAGKVKETGFAEFIMKLIPATAVSAFTSGDVLQVLL 158 Query: 158 FSMFFGIALASLGERGKHLLAVIDDLSHTMLKITVYVMKFAPVAVFAAMAATVAVNGLEI 217 S+ FG AL +GE+G ++A++ LS K + ++ AP+ V A+A TV G+ Sbjct: 159 ISVTFGCALLLIGEKGAPVVALVASLSDAFFKCMSFFIRLAPLGVLGAIAFTVGKYGIGS 218 Query: 218 LVSFAVFMRDFY-----FSLVLLWVILIAVGFIFLKKRIFHLLALIKEAFLLAFATASSE 272 L A+ + FY F LV+L IL A G IF L+ ++E ++ AT SS+ Sbjct: 219 LKQLALLVLLFYGAVIFFVLVVLGGILRASGL-----SIFKLIRYLREELVVVLATTSSD 273 Query: 273 AAYPKLLDALDRFGVKRKISSFVMPMGYSFNLDGSMIYCTFATLFIAQAYGIHMPLSTQI 332 + P+++ L+ G+K+ V+P GYSFNLD IY T A LFIAQA H+ + Sbjct: 274 SVLPQIMKKLEHMGIKKSTVGLVIPTGYSFNLDAFSIYLTMAALFIAQATNTHLSMGDLA 333 Query: 333 TMMLVLMLTSKGIAGVPRASLVVIAATLHQFN-IPEAGLLVILGIDTFLDMGRSATNAVG 391 ++ + ++TSKG GVP +++V++AATL IP GL+++L ID F+ + R+ N +G Sbjct: 334 AILAIALVTSKGAHGVPGSAIVILAATLTTIPAIPVVGLVLVLSIDWFIGIARALGNLLG 393 Query: 392 NSIASAVVAKWEGGLMTEEEAARAEVEIEQEAEAKLHTE 430 N +A+ V+A WE + + RA + E+ A L E Sbjct: 394 NCVATVVIASWE----RDIDKVRARAVLNGESVAPLVEE 428 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.139 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,106,848 Number of extensions: 41400 Number of successful extensions: 219 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 213 Number of HSP's successfully gapped: 3 Length of query: 431 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 343 Effective length of database: 1,203,190 Effective search space: 412694170 Effective search space used: 412694170 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)