BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0029.0240.p (483 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311040|ref|YP_003775132.1| major facilitator superfamily p... 870 0.0 gi|300310856|ref|YP_003774948.1| major facilitator superfamily p... 514 e-147 gi|300313188|ref|YP_003777280.1| major facilitator superfamily p... 319 3e-88 gi|300312683|ref|YP_003776775.1| major facilitator superfamily p... 241 1e-64 gi|300312017|ref|YP_003776109.1| multidrug resistance B (translo... 119 7e-28 >gi|300311040|ref|YP_003775132.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 441 Score = 870 bits (2249), Expect = 0.0, Method: Compositional matrix adjust. Identities = 440/441 (99%), Positives = 441/441 (100%) Query: 43 MDATVLATSLPALARDLHQDPITLKLAMTAYVAALGVFIPISGWMADKLGARKVFSAAMI 102 MDATVLATSLPALARDLHQDPITLKLAMTAYVAALGVFIPISGWMADKLGARKVFSAAMI Sbjct: 1 MDATVLATSLPALARDLHQDPITLKLAMTAYVAALGVFIPISGWMADKLGARKVFSAAMI 60 Query: 103 VFMTGSILCAVSGSLVTFVAARFLQGIGGAMMVPVGRVIIARSVDKSELVKAVSYLTLPA 162 VFMTGSILCAVSGSLVTFVAARFLQGIGGAMMVPVGRVIIARSVDKSELVKAVSYLTLPA Sbjct: 61 VFMTGSILCAVSGSLVTFVAARFLQGIGGAMMVPVGRVIIARSVDKSELVKAVSYLTLPA 120 Query: 163 LLGPVVGPLLGGFITTYFHWRWVFLINIPFCFLGLYLARRFIQNFKEDNPAPLDVKGFVL 222 LLGPVVGPLLGGFITTYFHWRWVFLINIPFCFLGLYLARRFIQNFKEDNPAPLDVKGFVL Sbjct: 121 LLGPVVGPLLGGFITTYFHWRWVFLINIPFCFLGLYLARRFIQNFKEDNPAPLDVKGFVL 180 Query: 223 TSVGAAMIMLGLSLFGSHLVDGATPVAMCSIGAIALVMYYRHSTQIEFPLLDFRLLRIPT 282 TSVGAAMIMLGLSLFGSHLVDGATPVAMCSIGAIALVMYYRHSTQIEFPLLDFRLLRIPT Sbjct: 181 TSVGAAMIMLGLSLFGSHLVDGATPVAMCSIGAIALVMYYRHSTQIEFPLLDFRLLRIPT 240 Query: 283 VHASVVGGSLFRIGLGAVPFLLPLALQEGLGMTAFQAGSITCASAFGSLFIRLLAGRVLR 342 +HASVVGGSLFRIGLGAVPFLLPLALQEGLGMTAFQAGSITCASAFGSLFIRLLAGRVLR Sbjct: 241 LHASVVGGSLFRIGLGAVPFLLPLALQEGLGMTAFQAGSITCASAFGSLFIRLLAGRVLR 300 Query: 343 RYGFRTVLMVNAVFAGIALAAYGIFSIHTPYLLIVSIVLMGGVFPALQFTCLNSIAYADI 402 RYGFRTVLMVNAVFAGIALAAYGIFSIHTPYLLIVSIVLMGGVFPALQFTCLNSIAYADI Sbjct: 301 RYGFRTVLMVNAVFAGIALAAYGIFSIHTPYLLIVSIVLMGGVFPALQFTCLNSIAYADI 360 Query: 403 DSRDAGRVTSLASVIQQLSLGLGVTVAGIVLQLSNHLQGHATIVEADFWPAFLVIGLFSV 462 DSRDAGRVTSLASVIQQLSLGLGVTVAGIVLQLSNHLQGHATIVEADFWPAFLVIGLFSV Sbjct: 361 DSRDAGRVTSLASVIQQLSLGLGVTVAGIVLQLSNHLQGHATIVEADFWPAFLVIGLFSV 420 Query: 463 ASVPISGKLPHNAGLELSRGK 483 ASVPISGKLPHNAGLELSRGK Sbjct: 421 ASVPISGKLPHNAGLELSRGK 441 >gi|300310856|ref|YP_003774948.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 446 Score = 514 bits (1323), Expect = e-147, Method: Compositional matrix adjust. Identities = 251/438 (57%), Positives = 331/438 (75%) Query: 43 MDATVLATSLPALARDLHQDPITLKLAMTAYVAALGVFIPISGWMADKLGARKVFSAAMI 102 MD+TV++TSLPALA D++ DPI+LKLA+T+Y+ +L VFIPISGWMAD+ G+ +F +A+ Sbjct: 1 MDSTVISTSLPALALDMNVDPISLKLALTSYLVSLAVFIPISGWMADRFGSLTIFRSAIC 60 Query: 103 VFMTGSILCAVSGSLVTFVAARFLQGIGGAMMVPVGRVIIARSVDKSELVKAVSYLTLPA 162 VFM GSILC VS +L FVAARFLQGIGGAMMVPVGR++I RSV+K LVKA+SYLT+PA Sbjct: 61 VFMLGSILCGVSSTLPQFVAARFLQGIGGAMMVPVGRLVILRSVEKVNLVKALSYLTIPA 120 Query: 163 LLGPVVGPLLGGFITTYFHWRWVFLINIPFCFLGLYLARRFIQNFKEDNPAPLDVKGFVL 222 L GPV+GP LGGFITTYFHWRW+F INIP LGLYLA RFI + K + APLD+KGF+L Sbjct: 121 LFGPVLGPPLGGFITTYFHWRWIFFINIPISILGLYLATRFIPDLKAEQVAPLDLKGFLL 180 Query: 223 TSVGAAMIMLGLSLFGSHLVDGATPVAMCSIGAIALVMYYRHSTQIEFPLLDFRLLRIPT 282 +++G +++M+GL+ G HLV G + G + L Y H+ + PL+D LL+IPT Sbjct: 181 SALGCSLLMMGLATAGRHLVSGEVALTCVLAGLVFLAAYVWHARRASAPLIDLALLKIPT 240 Query: 283 VHASVVGGSLFRIGLGAVPFLLPLALQEGLGMTAFQAGSITCASAFGSLFIRLLAGRVLR 342 + A V GGS +R+G+GA+PFLLPL LQ G G T F +G +TCASA G++F++ +A R LR Sbjct: 241 LRAGVFGGSFYRVGIGAIPFLLPLMLQLGFGYTPFHSGMLTCASAAGAMFMKTIAARFLR 300 Query: 343 RYGFRTVLMVNAVFAGIALAAYGIFSIHTPYLLIVSIVLMGGVFPALQFTCLNSIAYADI 402 RYGFR VL+VN + L A F+ TPYLL+V I+L+GG F +LQFT LN+I+YAD+ Sbjct: 301 RYGFRRVLLVNGALGVLGLWAIATFTPQTPYLLMVVILLIGGCFRSLQFTSLNAISYADV 360 Query: 403 DSRDAGRVTSLASVIQQLSLGLGVTVAGIVLQLSNHLQGHATIVEADFWPAFLVIGLFSV 462 D + TS+ASV QQLS+G+GV +AG LQ+SN +QGHAT+V DFWPAFLV+G + Sbjct: 361 DKLRLSQATSMASVAQQLSIGMGVVLAGFALQMSNEIQGHATLVAQDFWPAFLVVGAVAA 420 Query: 463 ASVPISGKLPHNAGLELS 480 ++ + +LPH+AG E++ Sbjct: 421 CALLMLRQLPHDAGAEMA 438 >gi|300313188|ref|YP_003777280.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 498 Score = 319 bits (817), Expect = 3e-88, Method: Compositional matrix adjust. Identities = 190/465 (40%), Positives = 283/465 (60%), Gaps = 16/465 (3%) Query: 29 LIPLIVGCALFMENMDATVLATSLPALARDLHQDPITLKLAMTAYVAALGVFIPISGWMA 88 ++P IV FME +D T++AT+LP +A+ P L L MTAY+ L VFIPISGW+A Sbjct: 31 MLPYIVAATFFMEYLDTTIIATALPQMAQSFGVGPNELSLGMTAYMLTLAVFIPISGWVA 90 Query: 89 DKLGARKVFSAAMIVFMTGSILCAVSGSLVTFVAARFLQGIGGAMMVPVGRVIIARSVDK 148 D+ G+R VF A+ +F SILC S ++ F AAR LQG+GGAMMVPVGR+I+ RS K Sbjct: 91 DRFGSRTVFGMAIAIFTLASILCGFSQNVWEFTAARVLQGLGGAMMVPVGRMIVVRSTGK 150 Query: 149 SELVKAVSYLTLPALLGPVVGPLLGGFITTYFHWRWVFLINIPFCFLGLYLARRFIQNFK 208 L+KA++ +T PA++ PVVGP +GGFITTY W W+FL+N+PF ++N Sbjct: 151 DRLMKAIATITWPAIVAPVVGPSIGGFITTYASWHWIFLMNVPFGLAAFTAVMMVVRNEH 210 Query: 209 EDNPAPLDVKGFVLTSVGAAMIMLGLSLFGSHLVDGATPVAMCSIGAIALVMY-----YR 263 + PLD+ GF+L+ GAA+ L L+G+ + ++G +AL + +R Sbjct: 211 GVSKRPLDLVGFLLS--GAALTSL---LYGTEIASHMESNIKVALGFVALGVVLGYGAWR 265 Query: 264 HSTQIEFPLLDFRLLRIPTVHASVVGGSLFRIGLGAVPFLLPLALQEGLGMTAFQAGSIT 323 H +IE PLLDF LRIPT +V+ GSL RIG+ AVP+L+PL Q G G+TAFQ+G + Sbjct: 266 HMQRIEHPLLDFSNLRIPTYSVTVLTGSLTRIGVDAVPYLMPLMFQIGFGLTAFQSGLLL 325 Query: 324 CASAFGSLFIRLLAGRVLRRYGFRTVLMVNAVFAGIALAAYGIFSIHTPYLLIVSIVLMG 383 A+A G+L ++ R+L+R+GFR+V + NA AG + A G+ + TP +I+ ++ Sbjct: 326 LATALGNLGMKAFTTRILQRFGFRSVAVNNAAIAGGLIIACGLLTPATPLPVILGVIFCY 385 Query: 384 GVFPALQFTCLNSIAYADIDSRDAGRVTSLASVIQQLSLGLGVTVAGIVLQLSNHLQGHA 443 G+ ++QF+ L ++AYADI ++L SV QQ+++G+G+ + L+++ L+G A Sbjct: 386 GLSRSMQFSTLATLAYADISDARKSSASTLWSVAQQMTIGMGIAFGALALRIAAALRGEA 445 Query: 444 TIVE------ADFWPAFLVIGLFSVASVPISGKLPHNAGLELSRG 482 DF AF + G+ ++ SV L +AG + G Sbjct: 446 IDASGAHFTLGDFQWAFAMAGVVTLISVIGYRGLARDAGSNIGGG 490 >gi|300312683|ref|YP_003776775.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 498 Score = 241 bits (614), Expect = 1e-64, Method: Compositional matrix adjust. Identities = 148/442 (33%), Positives = 246/442 (55%), Gaps = 9/442 (2%) Query: 33 IVGCALFMENMDATVLATSLPALARDLHQDPITLKLAMTAYVAALGVFIPISGWMADKLG 92 IV FM+ +D T++ T+LPA+AR L + + + + AY + P SGW+AD+ G Sbjct: 57 IVASGFFMQTLDTTIVNTALPAMARSLGEPVLNMHPVVVAYTLTMASLTPASGWLADRFG 116 Query: 93 ARKVFSAAMIVFMTGSILCAVSGSLVTFVAARFLQGIGGAMMVPVGRVIIARSVDKSELV 152 R+++ AA+++F GS+LCA++ SL +AAR +QG+GG+M++PVGR+ + R+V ++ + Sbjct: 117 TRRMYFAAILLFSLGSLLCAMASSLPMLLAARVVQGLGGSMLLPVGRLAVLRAVPGAQYI 176 Query: 153 KAVSYLTLPALLGPVVGPLLGGFITTYFHWRWVFLINIPFCFLGLYLARRFIQNFKEDNP 212 A++ +++ LGPVVGP+LGG++ W W+FLIN+P LG+ F+ + Sbjct: 177 AALASISIAGQLGPVVGPVLGGWLVESLSWHWIFLINLPVGVLGMGAVLYFLPDDSLAGT 236 Query: 213 APLDVKGFVLTSVGAAMIMLGLSL---FGSHLVDGATPVAMCSIGAIALVMYYRHSTQIE 269 D+ G VL S A M+ L+L F SH + +A+ + A+ ++ Y+RH++ Sbjct: 237 PRFDLLGCVLLS--ACMVAFSLALDVPFDSHRTGWS--IALFVVAALTVLGYWRHASVHP 292 Query: 270 FPLLDFRLLRIPTVHASVVGGSLFRIGLGAVPFLLPLALQEGLGMTAFQAGSITCASAFG 329 PL L P + G + RIG AVPFLLPL LQ LG + AG + +A Sbjct: 293 RPLFRLGLFGEPNFSIGLAGNLVCRIGSSAVPFLLPLLLQVRLGYSPLHAGMVLLPAAIA 352 Query: 330 SLFIRLLAGRVLRRYGFRTVLMVNAVFAGIALAAYGIFSIHTPYLLIVSIVLMGGVFPAL 389 + +++R+G+ L+VN G A+ ++ + + P + + + G ++ Sbjct: 353 GTIAKPWIAPLVKRFGYDRFLLVNTAIVGAAIMSFALITPDWPLTAAIVQLAIFGASNSM 412 Query: 390 QFTCLNSIAYADIDSRDAGRVTSLASVIQQLSLGLGVTVAGIVLQLSNHLQGHATIVEAD 449 QF +NS+ +D RDAG SL S++Q L++GLGVT+ G ++ L G AT A Sbjct: 413 QFAAMNSVTLKGLDKRDAGSGNSLFSMVQMLAIGLGVTIGGTMVNLFKSEVGTAT--NAS 470 Query: 450 FWPAFLVIGLFSVASVPISGKL 471 F F ++G ++AS + K+ Sbjct: 471 FQITFAIVGAITLASAWVFRKM 492 >gi|300312017|ref|YP_003776109.1| multidrug resistance B (translocase) transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 524 Score = 119 bits (297), Expect = 7e-28, Method: Compositional matrix adjust. Identities = 112/432 (25%), Positives = 200/432 (46%), Gaps = 31/432 (7%) Query: 32 LIVGCALFMENMDATVLATSLPALARDLHQDPITLKLAMTAYVAALGVFIPISGWMADKL 91 L V A+FM +D+++ ++P ++ +L +T++ AA + IP++GW+ +L Sbjct: 27 LSVSLAVFMNVLDSSIANVAIPTISGNLGVSVDEGTWVITSFAAANAISIPLTGWLTQRL 86 Query: 92 GARKVFSAAMIVFMTGSILCAVSGSLVTFVAARFLQGIGGAMMVPVGRVIIARSVDKSEL 151 GA ++F ++++F+ S LC ++ +L +AAR LQG+ MVP+ + ++ + KS+ Sbjct: 87 GAVRLFVTSVLLFVLASWLCGLAPTLPILLAARVLQGLVAGPMVPLSQSLLLGAYPKSKS 146 Query: 152 VKAVSYLTLPALLGPVVGPLLGGFITTYFHWRWVFLINIPFCF------LGLYLARRFIQ 205 A++ + A++ PV GP LGG+IT + W W+F INIP L +Y +R Sbjct: 147 SFALALWGMTAVVAPVAGPALGGWITDSYTWPWIFYINIPVGLIAAGVTLAIYRSRE--- 203 Query: 206 NFKEDNPAPLDVKGFVL--TSVGAAMIMLGLSLFGSHLVDGATP--VAMCSIGAIALVMY 261 + P+D G L T V A IML G L A+P V + I IA + Sbjct: 204 --TPTHKLPIDKIGLALLVTWVAAFQIMLDK---GKDLDWFASPTIVTLGIIALIAFAYF 258 Query: 262 YRHSTQIEFPLLDFRLLRIPTVHASVVGGSL-FRIGLGAV---PFLLPLALQEGLGMTAF 317 + P++D RL + GG++ IG G +LP LQ+ LG + Sbjct: 259 VVWELNNDHPVVDLRLFG----RRNFAGGTIAISIGYGVFFGNLVILPQWLQQYLGYRSI 314 Query: 318 QAGSITCASAFGSLFIRLLAGRVLRRYGFRTVLMVNAVFAGIALAAY--GIFSIHTPYLL 375 +G T ++ + L GR L R V A F G A+ + +++ + Sbjct: 315 DSGLSTAPIGIFAVILMPLIGRFLPLVDARKV--ATASFIGFAIVFWLRSRYNLQVDQMT 372 Query: 376 IVSIVLMGGVFPALQFTCLNSIAYADIDSRDAGRVTSLASVIQQLSLGLGVTVAGIVLQL 435 ++ L+ G+ A+ F L + + + L+S ++ +G +++ Sbjct: 373 VILPTLLQGIPTAMFFVPLTVLLLSGLPPERIPAAAGLSSFVRVFCGAVGTSISTTAWN- 431 Query: 436 SNHLQGHATIVE 447 + + HA + E Sbjct: 432 NRSIMHHAQLTE 443 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.328 0.143 0.427 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,466,530 Number of extensions: 62070 Number of successful extensions: 397 Number of sequences better than 1.0e-08: 20 Number of HSP's gapped: 381 Number of HSP's successfully gapped: 20 Length of query: 483 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 394 Effective length of database: 1,198,455 Effective search space: 472191270 Effective search space used: 472191270 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 132 (55.5 bits)