BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0029.0237.p (598 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311038|ref|YP_003775130.1| hypothetical protein Hsero_1709... 1214 0.0 gi|300310696|ref|YP_003774788.1| hypothetical protein Hsero_1364... 608 e-175 gi|300312012|ref|YP_003776104.1| phosphodiesterase transmembrane... 217 2e-57 gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodies... 195 1e-50 gi|300312026|ref|YP_003776118.1| signal transduction protein con... 193 4e-50 >gi|300311038|ref|YP_003775130.1| hypothetical protein Hsero_1709 [Herbaspirillum seropedicae SmR1] Length = 598 Score = 1214 bits (3141), Expect = 0.0, Method: Compositional matrix adjust. Identities = 595/598 (99%), Positives = 597/598 (99%) Query: 1 VLLEKEDNTEVEAAVRQESQRLAQLHGYCVLDTPPDKLCDQVTQLACSMFDVEIALVALV 60 +LLEKEDNTEVEAAVRQESQRLAQLHGYC LDTPPDKLCDQVTQLACSMFDVEIALVALV Sbjct: 1 MLLEKEDNTEVEAAVRQESQRLAQLHGYCALDTPPDKLCDQVTQLACSMFDVEIALVALV 60 Query: 61 DEHRQWFKSRVGLEVSETPRADSFCNHAIQTRHGMVVTDALQDARFSRHALVTGAPFIRF 120 DEHRQWFKSRVGLEVSETPRADSFCNHAIQTRHGMVVTDALQDARFSRHALVTGAPFIRF Sbjct: 61 DEHRQWFKSRVGLEVSETPRADSFCNHAIQTRHGMVVTDALQDARFSRHALVTGAPFIRF 120 Query: 121 YAGAPLVTPQGLSLGTLCIIDSRPRPDFGASQFAQLNQLAAIVMERLEQLRNRGYIDHVT 180 YAGAPLVTPQGLSLGTLCIIDSRPRPDFGASQFAQLNQLAAIVMERLEQLRNRGYIDHVT Sbjct: 121 YAGAPLVTPQGLSLGTLCIIDSRPRPDFGASQFAQLNQLAAIVMERLEQLRNRGYIDHVT 180 Query: 181 ALPNRARLMEVLRVRGHARPGASGADAAVAVDVCPREYLFDMVKALGWDYAEGFLMEVSR 240 ALPNRARLMEVLRVRGHARPGASGADAAVAVD+CPREYLFDMVKALGWDYAEGFLMEVSR Sbjct: 181 ALPNRARLMEVLRVRGHARPGASGADAAVAVDICPREYLFDMVKALGWDYAEGFLMEVSR 240 Query: 241 TLRRVLGTLPLYRVTTTTFVYLDETAPAQRRRRIADIEQRFTPALEFQGIPHLLDIAIGV 300 TLRRVLGTLPLYRVTTTTFVYLDETAPAQRRRRIADIEQRFTPALEFQGIPHLLDIAIGV Sbjct: 241 TLRRVLGTLPLYRVTTTTFVYLDETAPAQRRRRIADIEQRFTPALEFQGIPHLLDIAIGV 300 Query: 301 LPMEEDVSGGDVVRSLLTVIDVSREEGVSPRLFESVHAEKQRYAFWLLAALPMALTTPDE 360 LPMEEDVSGGDVVRSLLTVIDVSREEGVSPRLFESVHAEKQRYAFWLLAALPMALTTPDE Sbjct: 301 LPMEEDVSGGDVVRSLLTVIDVSREEGVSPRLFESVHAEKQRYAFWLLAALPMALTTPDE 360 Query: 361 LVLHFQPKVRLRDGACVGVEALIRWHHPIHGNIPPDVFIPLAEKTGQIRQVTAWVLRQVV 420 LVLHFQPKVRLRDGACVGVEALIRWHHPIHGNIPPDVFIPLAEKTGQIRQVTAWVLRQVV Sbjct: 361 LVLHFQPKVRLRDGACVGVEALIRWHHPIHGNIPPDVFIPLAEKTGQIRQVTAWVLRQVV 420 Query: 421 AQVARWLKSGRALPVAFNASARDFDSDELRELLAQQLKLHKVPPALIEIEFTEHAMANHP 480 AQVARWLKSGRALPVAFNASARDFDSDELRELLAQQLKLHKVPPALIEIEFTEHAMANHP Sbjct: 421 AQVARWLKSGRALPVAFNASARDFDSDELRELLAQQLKLHKVPPALIEIEFTEHAMANHP 480 Query: 481 QQVRQRVQALRELGVKVAIDDFGAGFSNLSYLKNIPADTMKIDRSFIHRLLDDEADQLIV 540 QQVRQRVQALRELGVKVAIDDFGAGFSNLSYLKNIPADTMKIDRSFIHRLLDDEADQLIV Sbjct: 481 QQVRQRVQALRELGVKVAIDDFGAGFSNLSYLKNIPADTMKIDRSFIHRLLDDEADQLIV 540 Query: 541 PSILHLAHNLGFSVVAEGLESERTATLLRHLGCDYGQGYAIARPMPLAELEAWLERRG 598 PSILHLAHNLGFSVVAEGLESERTATLLRHLGCDYGQGYAIARPMPLAELEAWLERRG Sbjct: 541 PSILHLAHNLGFSVVAEGLESERTATLLRHLGCDYGQGYAIARPMPLAELEAWLERRG 598 >gi|300310696|ref|YP_003774788.1| hypothetical protein Hsero_1364 [Herbaspirillum seropedicae SmR1] Length = 606 Score = 608 bits (1567), Expect = e-175, Method: Compositional matrix adjust. Identities = 316/588 (53%), Positives = 411/588 (69%), Gaps = 12/588 (2%) Query: 18 ESQRLAQLHGYCVLDTPPDKLCDQVTQLACSMFDVEIALVALVDEHRQWFKSRVGLEVSE 77 E QRLA L YC+LDT PD LCD +T+LA +FDVEIALV+LV EHRQWFKSR GL+ Sbjct: 22 EQQRLAVLDAYCLLDTQPDPLCDMITELATRLFDVEIALVSLVAEHRQWFKSRRGLDAPG 81 Query: 78 TPRADSFCNHAIQTRHGMVVTDALQDARFSRHALVTGAPFIRFYAGAPLVTPQGLSLGTL 137 TPR+ SFC HAI MV+ DA QD RF+ + LV G P IRFYAGA L T G++LGTL Sbjct: 82 TPRSQSFCAHAIVQAQTMVIPDARQDERFAGNPLVLGEPGIRFYAGAALRTADGVALGTL 141 Query: 138 CIIDSRPRPDFGASQFAQLNQLAAIVMERLEQLRNRGYIDHVTALPNRARLM-EVLRVRG 196 C+I PR F +L +LAAIVM RL+ LR Y+D +T+LPNR+RL+ +V ++ Sbjct: 142 CLIGKSPRSQFSEVDQDRLERLAAIVMARLDMLRMGSYVDSLTSLPNRSRLVADVQLMQA 201 Query: 197 HARPGASGAD------AAVAVDVCPREYLFDMVKALGWDYAEGFLMEVSRTLRRVLGTLP 250 HA G +G D AAVAVDVC YL DMVKALGWDY EGFL++ L + LG + Sbjct: 202 HA-TGLAGLDELPRRLAAVAVDVCDHTYLTDMVKALGWDYVEGFLIQAKERLCQALGEMA 260 Query: 251 LYRVTTTTFVYLDETAPAQRRRRIAD-IEQRFTPALEFQGIPHLLDIAIGVLPMEEDVSG 309 LYR+ T+F YLD R + AD I Q F +E QGIPH L++++G++P+ + Sbjct: 261 LYRIGATSFAYLDRC---DRWQDQADRIRQAFAATVEHQGIPHHLEVSVGIVPLSQCPDA 317 Query: 310 GDVVRSLLTVIDVSREEGVSPRLFESVHAEKQRYAFWLLAALPMALTTPDELVLHFQPKV 369 ++V SLLT DV+R +G +E + Q+++F LL+++P AL + D+L L FQPKV Sbjct: 318 AELVPSLLTAADVARADGHLQCQYEQPLGQMQKHSFELLSSIPAALLSTDQLWLEFQPKV 377 Query: 370 RLRDGACVGVEALIRWHHPIHGNIPPDVFIPLAEKTGQIRQVTAWVLRQVVAQVARWLKS 429 L G C GVEAL+RW HPIHG I P +FIPLAEKT IRQVT WVL++ + Q W + Sbjct: 378 SLSTGRCAGVEALLRWRHPIHGVISPALFIPLAEKTALIRQVTLWVLQRGIEQATAWARR 437 Query: 430 GRALPVAFNASARDFDSDELRELLAQQLKLHKVPPALIEIEFTEHAMANHPQQVRQRVQA 489 G A+PVA N SARDFD+D+L +LL + L+ H++P LIEIEFTE AM+ +P+++ R+Q Sbjct: 438 GVAIPVAINVSARDFDNDDLLQLLGRLLEQHRIPAGLIEIEFTESAMSRNPEKLYGRIQE 497 Query: 490 LRELGVKVAIDDFGAGFSNLSYLKNIPADTMKIDRSFIHRLLDDEADQLIVPSILHLAHN 549 ++ LGVKVAIDDFGAGFSNLSYLK+IPAD +K+D+SFI R+ D +DQ IVPS++HL Sbjct: 498 IKRLGVKVAIDDFGAGFSNLSYLKHIPADYLKLDQSFILRMESDYSDQQIVPSMIHLGQR 557 Query: 550 LGFSVVAEGLESERTATLLRHLGCDYGQGYAIARPMPLAELEAWLERR 597 LGF+VVAEG+E+E+T +L+HLGCD+GQG+ IARPM A+ W+ R Sbjct: 558 LGFAVVAEGVENEKTRRILQHLGCDFGQGFGIARPMNAADCWDWIGAR 605 >gi|300312012|ref|YP_003776104.1| phosphodiesterase transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 797 Score = 217 bits (553), Expect = 2e-57, Method: Compositional matrix adjust. Identities = 106/238 (44%), Positives = 147/238 (61%) Query: 359 DELVLHFQPKVRLRDGACVGVEALIRWHHPIHGNIPPDVFIPLAEKTGQIRQVTAWVLRQ 418 DE +L+ QPK+ L G VG EAL+RW HP G I PD FIP AE TG IR +T W++ Q Sbjct: 545 DEFMLYLQPKLELCSGQVVGAEALVRWIHPEKGFIGPDNFIPFAETTGFIRMLTLWMMEQ 604 Query: 419 VVAQVARWLKSGRALPVAFNASARDFDSDELRELLAQQLKLHKVPPALIEIEFTEHAMAN 478 V + + G L + N S RD EL A L+ H++ PA + +E TE A+ + Sbjct: 605 VARLSEQLQQQGVQLKFSVNLSTRDLLDQELPTAFAAILQRHRLQPAALCLEITESAIMD 664 Query: 479 HPQQVRQRVQALRELGVKVAIDDFGAGFSNLSYLKNIPADTMKIDRSFIHRLLDDEADQL 538 P + + ++ L +G ++IDDFG G+S+L+YLK +P D +KID+SF+ ++ D+ D Sbjct: 665 DPARAQNTLERLSNMGFALSIDDFGTGYSSLAYLKRLPVDELKIDKSFVMKMAQDQDDAK 724 Query: 539 IVPSILHLAHNLGFSVVAEGLESERTATLLRHLGCDYGQGYAIARPMPLAELEAWLER 596 IV S + L HNLG VVAEG+ESE LLR +GCD GQGY + +PMP+ AW E+ Sbjct: 725 IVRSTVDLGHNLGLRVVAEGVESEEVWFLLRQMGCDQGQGYFMGKPMPVQHFLAWREQ 782 >gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodiesterase with PAS/PAC sensor domains protein [Herbaspirillum seropedicae SmR1] Length = 701 Score = 195 bits (495), Expect = 1e-50, Method: Compositional matrix adjust. Identities = 148/443 (33%), Positives = 224/443 (50%), Gaps = 32/443 (7%) Query: 169 QLRNRGYIDHVTALPNRARLMEVLRVRGHARPGASGADAAVA-VDVCPREYLFDMVKALG 227 ++R++ D +T LPNR L E L+ R GA AVA VD+ +++ D +LG Sbjct: 261 EMRHQATHDALTGLPNRNMLHEHLQ-RALRVARQKGALTAVAFVDLDQFKFIND---SLG 316 Query: 228 WDYAEGFLMEVSRTLRRVLGTLPLY-RVTTTTFVYLDETAPAQRRRRIADIEQRFTPAL- 285 + L V++ L+ L + R FV + + + IA++ QR A+ Sbjct: 317 HQVGDELLKTVAQRLQACLREADMVARQGGDEFVLVLQN-QTEGEAGIAEVMQRILAAVA 375 Query: 286 --------EFQGIPHLLDIAIGVLPMEEDVSGGDVVRSLLTVIDVS----REEGVSPRLF 333 EFQ + +IGV D G V SLL D + +E+G + F Sbjct: 376 RPWQAGDREFQ-----VTASIGVSRYPAD---GKDVESLLKHADSAMYRAKEQGRNNFQF 427 Query: 334 ESVHAEKQ-RYAFWLLAALPMALTTPDELVLHFQPKVRLRDGACVGVEALIRWHHPIHGN 392 + + Q +L L AL DE L++QPK+ L+DG +G EALIRW P G Sbjct: 428 FAPWMDSQVSNRLEMLINLRRALDQ-DEFKLYYQPKLSLQDGRLIGAEALIRWESPQQGM 486 Query: 393 IPPDVFIPLAEKTGQIRQVTAWVLRQVVAQVARWLKSGRA-LPVAFNASARDFDSDELRE 451 +PPD FIP AE+ G I + WVLR Q W ++G +PVA N S R + L + Sbjct: 487 VPPDRFIPFAEEAGLIVPIGEWVLRTACRQNQAWQQAGLPPIPVAVNLSPRQLNQS-LPD 545 Query: 452 LLAQQLKLHKVPPALIEIEFTEHAMANHPQQVRQRVQALRELGVKVAIDDFGAGFSNLSY 511 +A L + + +E+E TE+ + ++ + AL+ LG+++++DDFG G+S+LSY Sbjct: 546 FVAGVLAQSGLAASWLELEITENVVMKDAEKSVATLHALKRLGLQISVDDFGTGYSSLSY 605 Query: 512 LKNIPADTMKIDRSFIHRLLDDEADQLIVPSILHLAHNLGFSVVAEGLESERTATLLRHL 571 L+ P D +KID+SF+ ++ D IV +I+ LAH L V+AEG+E E L+ Sbjct: 606 LRRFPVDALKIDKSFVRDIVRDADSAAIVKAIISLAHILNLRVIAEGVEDEEQHAFLKEN 665 Query: 572 GCDYGQGYAIARPMPLAELEAWL 594 CD QGY +PM +A AW+ Sbjct: 666 ACDEVQGYYFGKPMEVAVFTAWM 688 >gi|300312026|ref|YP_003776118.1| signal transduction protein containing a membrane domain, an EAL and a GGDEF domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 1106 Score = 193 bits (490), Expect = 4e-50, Method: Compositional matrix adjust. Identities = 145/495 (29%), Positives = 241/495 (48%), Gaps = 51/495 (10%) Query: 125 PLVTPQGLSLGTLC-IIDSRPRPDFGASQFAQLN-QLAAIVMERL---EQLRNRGYIDHV 179 P++ +G LG ++ P Q A + LA++ +E E++R+ + D + Sbjct: 616 PIIGKEGQLLGVFAALLKENQAPSNEYLQLANIAADLASVAIESRRADERIRHLAHYDEL 675 Query: 180 TALPNRARLMEVLRVRGHARPGASGADAAVAVDVCPREYLFDMVKALGWDYAEGFLMEVS 239 T LPNR + + +A A + VAV + + ++ G + E L E++ Sbjct: 676 TGLPNRFLCTQHV---SNAITHAEHRNGMVAVILMDLDRFKNINDTFGHETGEAVLQEIA 732 Query: 240 RTLRRVLGTLP-LYRVTTTTFVYL--DETAPAQRRRRIADIEQRF-TPALE---FQGIPH 292 + R L L L RV F+ L D P Q + +I Q+ T A + G Sbjct: 733 QRFRNSLRELDILARVGGDEFIVLVDDFDDPVQ----LGEIAQKLLTEARKPFIIDGQEA 788 Query: 293 LLDIAIGVLPMEEDVSGGDVVRSLLTVIDVSREEGVSPRLFESVHAEKQRYAFW------ 346 +L ++IG+ D GD ++L+ D++ ++ + H K Y F+ Sbjct: 789 MLSVSIGIATYPGD---GDNAQTLIKNADIA--------MYRAKHHGKDDYRFFSDEINT 837 Query: 347 -------LLAALPMALTTPDELVLHFQPKVRLRDGACVGVEALIRWHHPIHGNIPPDVFI 399 L A L A+ E V+H+QPK+ L +G VG EAL+RW HP+ G +PP FI Sbjct: 838 NTVERIALEAELRRAIERA-EFVVHYQPKIELAEGRIVGAEALVRWQHPLRGLLPPGEFI 896 Query: 400 PLAEKTGQIRQVTAWVL----RQVVAQVARWLKSGRALPVAFNASARDFDSDELRELLAQ 455 LAE+T I Q+ VL R + + + GR ++ N + F + L + + + Sbjct: 897 TLAEETRLIDQIGLLVLDVACRDIGLMRQQQMTCGR---ISINLTGSQFGDERLLDDIKE 953 Query: 456 QLKLHKVPPALIEIEFTEHAMANHPQQVRQRVQALRELGVKVAIDDFGAGFSNLSYLKNI 515 + H PP+ +E E TE + ++ ++ + +R G ++IDDFG G+S+L+YLK Sbjct: 954 VVDRHGTPPSCLEFEITESMVMHNRERAIAIMDGIRAEGFTISIDDFGTGYSSLAYLKRF 1013 Query: 516 PADTMKIDRSFIHRLLDDEADQLIVPSILHLAHNLGFSVVAEGLESERTATLLRHLGCDY 575 P +T+KID+SFI+ + D+ IV +I+ ++H L VV EG+E+E+ LR G D Sbjct: 1014 PVNTLKIDKSFINDIPDNPNSSAIVQAIIAMSHALNLKVVTEGVETEKQLQALREFGSDE 1073 Query: 576 GQGYAIARPMPLAEL 590 QGY ++P+P A+ Sbjct: 1074 YQGYYFSKPVPYAQF 1088 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.138 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,873,335 Number of extensions: 77771 Number of successful extensions: 263 Number of sequences better than 1.0e-08: 23 Number of HSP's gapped: 239 Number of HSP's successfully gapped: 23 Length of query: 598 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 507 Effective length of database: 1,188,985 Effective search space: 602815395 Effective search space used: 602815395 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 133 (55.8 bits)