BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0029.0235.p (750 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311036|ref|YP_003775128.1| signal transduction protein [He... 1528 0.0 gi|300314042|ref|YP_003778134.1| signal transduction protein con... 397 e-111 gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodies... 386 e-108 gi|300311871|ref|YP_003775963.1| EAL domain-containing protein [... 351 2e-97 gi|300312606|ref|YP_003776698.1| EAL domain transmembrane protei... 337 2e-93 >gi|300311036|ref|YP_003775128.1| signal transduction protein [Herbaspirillum seropedicae SmR1] Length = 750 Score = 1528 bits (3957), Expect = 0.0, Method: Compositional matrix adjust. Identities = 748/750 (99%), Positives = 748/750 (99%) Query: 1 MNQDVLRDISEKYCGLLRAFLLSPEGNAASEQLQLAHHLGGSLVMGRIAPAELLGIHQAA 60 MNQDVLRDISEKYCGLLRAFLLSPEGNAASEQLQLAHHLGGSLVMGRIAPAELLGIHQAA Sbjct: 1 MNQDVLRDISEKYCGLLRAFLLSPEGNAASEQLQLAHHLGGSLVMGRIAPAELLGIHQAA 60 Query: 61 VESLANCCPALPLAQIGARLNMPLVTMYQAYESAFRERIAVRYQDLAASFFCASGEQELI 120 VESLANCCPAL LAQIGARLNMPLVTMYQAYESAFRERIAVRYQDLAASFFCASGEQELI Sbjct: 61 VESLANCCPALQLAQIGARLNMPLVTMYQAYESAFRERIAVRYQDLAASFFCASGEQELI 120 Query: 121 ARQIGDVFNDFNTHLGSIIGFAEMVEDELSRKSSGQQSLHLLMQNLTALLKAEHAIQQRI 180 ARQIGDVFNDFNTHLGSIIGFAEMVEDELSRKSSGQQSLHLLMQNLTALLKAEHAIQQRI Sbjct: 121 ARQIGDVFNDFNTHLGSIIGFAEMVEDELSRKSSGQQSLHLLMQNLTALLKAEHAIQQRI 180 Query: 181 RSSERQFRTLVDNSPDVILRYDMEGRCVFANPAFTRETGLMADQDINTILDRSANRRWDD 240 RSSERQFRTLVDNSPDVILRYDMEGRCVFANPAFTRETGLMADQDINTILDRSANRRWDD Sbjct: 181 RSSERQFRTLVDNSPDVILRYDMEGRCVFANPAFTRETGLMADQDINTILDRSANRRWDD 240 Query: 241 ESCSFGERVRQVAATGVADVVVLEWRHPQGHLVSHEMHIVPEYDADEQIFGTLAIGRDVT 300 ESCSFGERVRQVAATGVADVVVLEWRHPQGHLVSHEMHIVPEYDADEQIFGTLAIGRDVT Sbjct: 241 ESCSFGERVRQVAATGVADVVVLEWRHPQGHLVSHEMHIVPEYDADEQIFGTLAIGRDVT 300 Query: 301 RRKEVEFKLRHQANYDPLSGLPNRRLLGDMLQREVASAKKHAYPLAVVFIDLDRFKDVND 360 RRKEVEFKLRHQANYDPLSGLPNRRLLGDMLQREVASAKKHAYPLAVVFIDLDRFKDVND Sbjct: 301 RRKEVEFKLRHQANYDPLSGLPNRRLLGDMLQREVASAKKHAYPLAVVFIDLDRFKDVND 360 Query: 361 SLGHSVGDQLLVVAAQRVRASVREHDLVARLAGDEFVAVLPRLEASAALTRIGDDIVAAL 420 SLGHSVGDQLLVVAAQRVRASVREHDLVARLAGDEFVAVLPRLEASAALTRIGDDIVAAL Sbjct: 361 SLGHSVGDQLLVVAAQRVRASVREHDLVARLAGDEFVAVLPRLEASAALTRIGDDIVAAL 420 Query: 421 AEPFQLGEHRVRISASVGIAIYPHDADTVTTLIGCADQAMFAAKGGGRNGLAFFTEDMRE 480 AEPFQLGEHRVRISASVGIAIYPHDADTVTTLIGCADQAMFAAKGGGRNGLAFFTEDMRE Sbjct: 421 AEPFQLGEHRVRISASVGIAIYPHDADTVTTLIGCADQAMFAAKGGGRNGLAFFTEDMRE 480 Query: 481 KSFERRRLLNDLYDALQEGQFELHFQPALELQSGVIHKAEALVRWRHPAFGMISPDRFIP 540 KSFERRRLLNDLYDALQEGQFELHFQPALELQSGVIHKAEALVRWRHPAFGMISPDRFIP Sbjct: 481 KSFERRRLLNDLYDALQEGQFELHFQPALELQSGVIHKAEALVRWRHPAFGMISPDRFIP 540 Query: 541 LAEESGLIFDLGRWILQEAARVSVRWNAQGTSPFRKQISVNLSPVQFLNGHYDAIVMDQL 600 LAEESGLIFDLGRWILQEAARVSVRWNAQGTSPFRKQISVNLSPVQFLNGHYDAIVMDQL Sbjct: 541 LAEESGLIFDLGRWILQEAARVSVRWNAQGTSPFRKQISVNLSPVQFLNGHYDAIVMDQL 600 Query: 601 SLQGCQPDLIAIEITEGLLLDDSEAVRSQLNNLRAAGIQVALDDFGTGYSAMSYLKKFSM 660 SLQGCQP LIAIEITEGLLLDDSEAVRSQLNNLRAAGIQVALDDFGTGYSAMSYLKKFSM Sbjct: 601 SLQGCQPGLIAIEITEGLLLDDSEAVRSQLNNLRAAGIQVALDDFGTGYSAMSYLKKFSM 660 Query: 661 DYLKIDRSFVQDMVDADSNKAIVEAIIVMAHRLGMKVIAEGVETMVQAQLLRDAGCEYVQ 720 DYLKIDRSFVQDMVDADSNKAIVEAIIVMAHRLGMKVIAEGVETMVQAQLLRDAGCEYVQ Sbjct: 661 DYLKIDRSFVQDMVDADSNKAIVEAIIVMAHRLGMKVIAEGVETMVQAQLLRDAGCEYVQ 720 Query: 721 GYLYSRPLPEDDFLNFAASAHSPADAAAAR 750 GYLYSRPLPEDDFLNFAASAHSPADAAAAR Sbjct: 721 GYLYSRPLPEDDFLNFAASAHSPADAAAAR 750 >gi|300314042|ref|YP_003778134.1| signal transduction protein containing a membrane domain, an EAL and a GGDEF domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 948 Score = 397 bits (1021), Expect = e-111, Method: Compositional matrix adjust. Identities = 236/577 (40%), Positives = 330/577 (57%), Gaps = 18/577 (3%) Query: 161 LLMQNLTALLKAEHAIQ---QRIRSSERQFRTLVDNSPDVILRYDMEGRCVFANPAFTRE 217 LL ++ A AE A++ Q I +S L PD + Y NPAF R Sbjct: 375 LLSDDIKARRLAERALRLRHQAIEASANAILILSAQEPDYTIEY--------VNPAFARI 426 Query: 218 TGLMADQDINTILDRSANRRWDDESCSFGERVRQVAATGVADVVVLEWRHPQGHLVSHEM 277 TG + + + + R D E E V VVL H G + ++ Sbjct: 427 TGYSSTEAVGKSIRFLEGRDSDPEGF---ETVWIAMREQREHNVVLRNYHKDGSELWNDF 483 Query: 278 HIVPEYDADEQIFGTLAIGRDVTRRKEVEFKLRHQANYDPLSGLPNRRLLGDMLQREVAS 337 +I P D ++ +A D+T K E +L QA+ D L+GL NR +L L++ + Sbjct: 484 YIAPVRDESGEVTHFVAALYDITSMKRYEAELEFQASRDTLTGLINRHMLRHHLEQAIGV 543 Query: 338 AKKHAYPLAVVFIDLDRFKDVNDSLGHSVGDQLLVVAAQRVRASVREHDLVARLAGDEFV 397 A++ L VVF+DLDRFK VND+LGH GD LL A R+RA+VRE D VARL GDEF+ Sbjct: 544 AERQKQALWVVFVDLDRFKFVNDTLGHKAGDTLLRTVAGRLRAAVREADTVARLGGDEFI 603 Query: 398 AVLPRLEASAALTRIGDDIVAALAEPFQLGEHRVRISASVGIAIYPHDADTVTTLIGCAD 457 +LP + TR I+ A+A P LG H + ++ SVG+A YP+D TLI AD Sbjct: 604 MILPEQDELTLDTRSLQRIMDAVAAPVTLGGHTLFMTCSVGVACYPNDGTDGETLIKHAD 663 Query: 458 QAMFAAKGGGRNGLAFFTEDMREKSFERRRLLNDLYDALQEGQFELHFQPALELQSGVIH 517 AM+ K GRN F+T M E++ ER R+ DL +AL G+F LH+QP ++L SG + Sbjct: 664 IAMYRGKQTGRNNFQFYTPAMNEQALERLRIEGDLRNALDHGEFLLHYQPKVDLASGRVI 723 Query: 518 KAEALVRWRHPAFGMISPDRFIPLAEESGLIFDLGRWILQEAARVSVRWNAQGTSPFRKQ 577 +EAL+RW+HP GM+SP RFI LAEE+GLI +G W+L+ A +W G + Sbjct: 724 GSEALIRWQHPQLGMVSPARFIGLAEETGLILPIGAWVLRAACTQCRQWQLGGYADI--S 781 Query: 578 ISVNLSPVQFLNGHYDAIVMDQLSLQGCQPDLIAIEITEGLLLDDSEAVRSQLNNLRAAG 637 +SVNLS QFL + + D L G P+ + +E+TE L++ D E L L+ G Sbjct: 782 VSVNLSVGQFLQKDLVSSIADVLEDTGLPPESLELELTESLMMTDVEHAIGILAGLKKLG 841 Query: 638 IQVALDDFGTGYSAMSYLKKFSMDYLKIDRSFVQDM-VDADSNKAIVEAIIVMAHRLGMK 696 +++++DDFGTGYS+++YLK+F +D LKIDRSFV+D+ +DAD + AI +II +A L ++ Sbjct: 842 VRLSIDDFGTGYSSLAYLKRFPIDVLKIDRSFVRDITIDAD-DAAITASIISLARHLKLR 900 Query: 697 VIAEGVETMVQAQLLRDAGCEYVQGYLYSRPLPEDDF 733 VIAEGVET Q LR GC+ +QGYL+S P+P D F Sbjct: 901 VIAEGVETDEQLDYLRHYGCDEIQGYLFSPPVPADKF 937 >gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodiesterase with PAS/PAC sensor domains protein [Herbaspirillum seropedicae SmR1] Length = 701 Score = 386 bits (992), Expect = e-108, Method: Compositional matrix adjust. Identities = 225/580 (38%), Positives = 344/580 (59%), Gaps = 12/580 (2%) Query: 152 KSSGQ-QSLHLLMQNLTALLKAEHAIQQRIRSSERQFRTLVDNSPDVILRYDMEGRCVFA 210 ++GQ +++ +Q++T +A A+Q+ +ER++R++ +N+ + + + +G + Sbjct: 109 NANGQVEAIEGFLQDVTERKEAAQALQE----AERRYRSIFENAIEGVFQTTPDGTYIAV 164 Query: 211 NPAFTRETGLMADQDINTILDRSANRRWDDESCSFGERVRQVAATGVADVVVLEWRHPQG 270 NPA R G + +D+ L R + + E E +R + G G Sbjct: 165 NPALARIYGYHSPEDLIVGL-RDISHQLYVEPERRQEFMRLMEEQGSVSNFESRVYRRDG 223 Query: 271 HLVSHEMHIVPEYDADEQIFGTLAIGRDVTRRKEVEFKLRHQANYDPLSGLPNRRLLGDM 330 ++ + YD ++ +T RK E ++RHQA +D L+GLPNR +L + Sbjct: 224 EIIWISENARAVYDEAGKLSCYEGTVEAITERKAHEAEMRHQATHDALTGLPNRNMLHEH 283 Query: 331 LQREVASAKKHAYPLAVVFIDLDRFKDVNDSLGHSVGDQLLVVAAQRVRASVREHDLVAR 390 LQR + A++ AV F+DLD+FK +NDSLGH VGD+LL AQR++A +RE D+VAR Sbjct: 284 LQRALRVARQKGALTAVAFVDLDQFKFINDSLGHQVGDELLKTVAQRLQACLREADMVAR 343 Query: 391 LAGDEFVAVLP-RLEASAALTRIGDDIVAALAEPFQLGEHRVRISASVGIAIYPHDADTV 449 GDEFV VL + E A + + I+AA+A P+Q G+ +++AS+G++ YP D V Sbjct: 344 QGGDEFVLVLQNQTEGEAGIAEVMQRILAAVARPWQAGDREFQVTASIGVSRYPADGKDV 403 Query: 450 TTLIGCADQAMFAAKGGGRNGLAFFTEDMREKSFERRRLLNDLYDALQEGQFELHFQPAL 509 +L+ AD AM+ AK GRN FF M + R +L +L AL + +F+L++QP L Sbjct: 404 ESLLKHADSAMYRAKEQGRNNFQFFAPWMDSQVSNRLEMLINLRRALDQDEFKLYYQPKL 463 Query: 510 ELQSGVIHKAEALVRWRHPAFGMISPDRFIPLAEESGLIFDLGRWILQEAARVSVRWNAQ 569 LQ G + AEAL+RW P GM+ PDRFIP AEE+GLI +G W+L+ A R + W Sbjct: 464 SLQDGRLIGAEALIRWESPQQGMVPPDRFIPFAEEAGLIVPIGEWVLRTACRQNQAWQQA 523 Query: 570 GTSPFRKQISVNLSPVQFLNGHYDAIVMDQLSLQGCQPDLIAIEITEGLLLDDSEAVRSQ 629 G P ++VNLSP Q LN V L+ G + +EITE +++ D+E + Sbjct: 524 GLPPI--PVAVNLSPRQ-LNQSLPDFVAGVLAQSGLAASWLELEITENVVMKDAEKSVAT 580 Query: 630 LNNLRAAGIQVALDDFGTGYSAMSYLKKFSMDYLKIDRSFVQDMV-DADSNKAIVEAIIV 688 L+ L+ G+Q+++DDFGTGYS++SYL++F +D LKID+SFV+D+V DADS AIV+AII Sbjct: 581 LHALKRLGLQISVDDFGTGYSSLSYLRRFPVDALKIDKSFVRDIVRDADS-AAIVKAIIS 639 Query: 689 MAHRLGMKVIAEGVETMVQAQLLRDAGCEYVQGYLYSRPL 728 +AH L ++VIAEGVE Q L++ C+ VQGY + +P+ Sbjct: 640 LAHILNLRVIAEGVEDEEQHAFLKENACDEVQGYYFGKPM 679 >gi|300311871|ref|YP_003775963.1| EAL domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 1036 Score = 351 bits (900), Expect = 2e-97, Method: Compositional matrix adjust. Identities = 211/552 (38%), Positives = 314/552 (56%), Gaps = 14/552 (2%) Query: 193 NSPDVILRYDMEGRCVFANPAFTRETGLMADQDINTILDRSANR-RWDDESCSFGERVRQ 251 +S + +L D + + N AFT TG A++ I+ + N R D +F + +R+ Sbjct: 482 DSREGMLITDANSKILRVNKAFTEITGYSAEE----IVGKHPNLLRSDRHGPAFFQEMRE 537 Query: 252 -VAATGVADVVVLEWRHPQGHLVSHEMHIVPEYDADEQIFGTLAIGRDVTRRKEVEFKLR 310 + G + + R G + + I + D+Q+ ++ D++ RK E ++R Sbjct: 538 SIRRFGKWQGEIWD-RRKNGEVYPEWLTISAVKNKDQQVTHYVSTHHDISDRKLAEERIR 596 Query: 311 HQANYDPLSGLPNRRLLGDMLQREVASAKKHAYPLAVVFIDLDRFKDVNDSLGHSVGDQL 370 A +D L+ LPNR LL D L++ +A + + A++FIDLD FK +ND++GH GD L Sbjct: 597 ELAFFDALTRLPNRTLLLDRLKQAIALSLRTKTCAALLFIDLDHFKTLNDTIGHEKGDTL 656 Query: 371 LVVAAQRVRASVREHDLVARLAGDEFVAVLPRL-----EASAALTRIGDDIVAALAEPFQ 425 L AQR+ A+VRE+D VAR+ GDEFV VL L EA+ IG+ I+ AL +Q Sbjct: 657 LKQVAQRLLANVRENDTVARVGGDEFVVVLEGLNELQQEAATQTRAIGEKILTALGSTYQ 716 Query: 426 LGEHRVRISASVGIAIYPHDADTVTTLIGCADQAMFAAKGGGRNGLAFFTEDMREKSFER 485 L + R +AS+G ++ A +V L+ AD AM+ AK GRN L FF M+ ER Sbjct: 717 LDDVEYRTTASIGATVFLGRAASVDELMKQADLAMYKAKETGRNALRFFDPAMQTAVLER 776 Query: 486 RRLLNDLYDALQEGQFELHFQPALELQSGVIHKAEALVRWRHPAFGMISPDRFIPLAEES 545 L L A+ Q LH+QP + ++ G + AE LVRW+HP GM+ P FIPLAEE+ Sbjct: 777 ATLEAGLRKAIDGNQLLLHYQPQV-VEGGRVTGAEVLVRWQHPQRGMVPPGDFIPLAEET 835 Query: 546 GLIFDLGRWILQEAARVSVRWNAQGTSPFRKQISVNLSPVQFLNGHYDAIVMDQLSLQGC 605 GLI LG W+L+ A R W AQ I+VN+S Q + V+D + G Sbjct: 836 GLILSLGNWVLETACRQLATWAAQPQMA-DLSIAVNVSAPQLREPDFVQTVLDTIERTGA 894 Query: 606 QPDLIAIEITEGLLLDDSEAVRSQLNNLRAAGIQVALDDFGTGYSAMSYLKKFSMDYLKI 665 P + +E+TE LL+D+ E + ++ L+A G++ +LDDFGTGYS++SYLK+ ++ LKI Sbjct: 895 NPRRLKLELTESLLVDNVEDIIQKMFALKAYGVEFSLDDFGTGYSSLSYLKRLPLNQLKI 954 Query: 666 DRSFVQDMVDADSNKAIVEAIIVMAHRLGMKVIAEGVETMVQAQLLRDAGCEYVQGYLYS 725 D+SFV+D++ ++ +I + I+ +A LG+ VIAEGVET Q L DAGC QGY + Sbjct: 955 DQSFVRDVLIDPNDASIAKTIVNLAQSLGLGVIAEGVETDAQRSFLADAGCHAYQGYFFC 1014 Query: 726 RPLPEDDFLNFA 737 RP+ + F FA Sbjct: 1015 RPVTVEAFEKFA 1026 >gi|300312606|ref|YP_003776698.1| EAL domain transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 695 Score = 337 bits (864), Expect = 2e-93, Method: Compositional matrix adjust. Identities = 185/437 (42%), Positives = 262/437 (59%), Gaps = 3/437 (0%) Query: 308 KLRHQANYDPLSGLPNRRLLGDMLQREVASAKKHAYPLAVVFIDLDRFKDVNDSLGHSVG 367 +L +D L+ LPNR LL D L + + A + A++F+DLD FK +NDSLGH VG Sbjct: 261 ELTQMVLHDQLTRLPNRTLLEDRLNQAINKAAREQGHFALMFMDLDGFKAINDSLGHHVG 320 Query: 368 DQLLVVAAQRVRASVREHDLVARLAGDEFVAVLPRLEASAALTRIGDDIVAALAEPFQLG 427 D+LLV AQR+ +S R +D VARL GDEFV +L L + + +V A+ +PF++ Sbjct: 321 DRLLVEVAQRLTSSSRANDTVARLGGDEFV-ILVDLNTPEDAMGVAEKLVEAVNQPFKVD 379 Query: 428 EHRVRISASVGIAIYPHDADTVTTLIGCADQAMFAAKGGGRNGLAFFTEDMREKSFERRR 487 +H +R+SASVGIAIYP D T L+ AD AM+ K GRNG FF M + ++ + Sbjct: 380 QHELRVSASVGIAIYPEDGATRHDLVINADAAMYHTKRSGRNGYHFFEPSMNANAQQQLQ 439 Query: 488 LLNDLYDALQEGQFELHFQPALELQSGVIHKAEALVRWRHPAFGMISPDRFIPLAEESGL 547 L DL AL+ GQF L +QP L +G + AEAL+RW HP G+++PD+FI LAE SGL Sbjct: 440 WLQDLRVALERGQFRLVYQPKFSLPAGPVLGAEALLRWEHPVHGLVAPDQFIGLAERSGL 499 Query: 548 IFDLGRWILQEAARVSVRWNAQGTSPFRKQISVNLSPVQFLNGHYDAIVMDQLSLQGCQP 607 I +G W+L EA R W A G + +SVNLS +QF + H +V L P Sbjct: 500 ILPIGAWVLDEACRQMREWFALGYVDW--TMSVNLSALQFADPHLLEVVRTTLERHQLPP 557 Query: 608 DLIAIEITEGLLLDDSEAVRSQLNNLRAAGIQVALDDFGTGYSAMSYLKKFSMDYLKIDR 667 + +E+TE + D+EA L + G+++++DDFGTGYS++ YLK+ + LKIDR Sbjct: 558 PCLTLEVTESTAMHDTEASLQTLRQIADLGVEISIDDFGTGYSSLLYLKRLPANELKIDR 617 Query: 668 SFVQDMVDADSNKAIVEAIIVMAHRLGMKVIAEGVETMVQAQLLRDAGCEYVQGYLYSRP 727 FV+++ + AIV AI+ + L ++++AEGVET+ Q L AGC+ +QGYL RP Sbjct: 618 GFVRELRQDGEDAAIVSAIVALGRTLNLRIVAEGVETVEQQDFLTAAGCDALQGYLMGRP 677 Query: 728 LPEDDFLNFAASAHSPA 744 + + FL H A Sbjct: 678 MTPELFLQTMRERHKSA 694 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.136 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,210,932 Number of extensions: 93063 Number of successful extensions: 440 Number of sequences better than 1.0e-08: 45 Number of HSP's gapped: 375 Number of HSP's successfully gapped: 45 Length of query: 750 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 658 Effective length of database: 1,184,250 Effective search space: 779236500 Effective search space used: 779236500 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 134 (56.2 bits)