BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0029.0231.p (834 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311033|ref|YP_003775125.1| chemotaxis histidine kinase [He... 1546 0.0 gi|300309957|ref|YP_003774049.1| chemotaxis histidine kinase [He... 255 1e-68 gi|300312286|ref|YP_003776378.1| signal-transducing histidine ki... 236 5e-63 gi|300312396|ref|YP_003776488.1| chemotaxis CheA protein [Herbas... 232 1e-61 gi|300312322|ref|YP_003776414.1| two component hybrid sensor his... 189 1e-48 >gi|300311033|ref|YP_003775125.1| chemotaxis histidine kinase [Herbaspirillum seropedicae SmR1] Length = 837 Score = 1546 bits (4004), Expect = 0.0, Method: Compositional matrix adjust. Identities = 813/839 (96%), Positives = 816/839 (97%), Gaps = 7/839 (0%) Query: 1 MTLLRDQFIIETRDLLQGISEKLMQLEDAPEHVELLESLFRMVHTLKGNSGLFDVPEMTR 60 MTLLRDQFI+ETRDLLQGISEKLMQLEDAPEHVELLESLFRMVHTLKGNSGLFDVPEMTR Sbjct: 1 MTLLRDQFILETRDLLQGISEKLMQLEDAPEHVELLESLFRMVHTLKGNSGLFDVPEMTR 60 Query: 61 VLHACEDLMDAVRRRQVAYSEVLADRLLDAMDFVAMLCDGLEMEAEQADPHAQEAVQIAS 120 VLHACEDLMDAVRRRQVAYSEVLADRLLDAMDFVAMLCDGLEMEAEQADPHAQEAVQIAS Sbjct: 61 VLHACEDLMDAVRRRQVAYSEVLADRLLDAMDFVAMLCDGLEMEAEQADPHAQEAVQIAS 120 Query: 121 SLRQLIGEHAGVESAVGDLARIGDAQDRGAAIVAAPAAGQALPLRAIPEPLRRQAFAQAQ 180 SLRQLIGEHAGVESAVGDLARIGDAQDRGAAIVAAPAAGQALPLRAIPEPLRRQAFAQAQ Sbjct: 121 SLRQLIGEHAGVESAVGDLARIGDAQDRGAAIVAAPAAGQALPLRAIPEPLRRQAFAQAQ 180 Query: 181 AGGELHWIAYRPSRECFFQGDDPFHRAREVPGFIWGRIDAPAGWPPLAELNAYDCVLHFQ 240 AGGELHWIAYRPSRECFFQGDDPFHRAREVPGFIWGRI+APA WPPLAELNAYDCVLHFQ Sbjct: 181 AGGELHWIAYRPSRECFFQGDDPFHRAREVPGFIWGRIEAPAAWPPLAELNAYDCVLHFQ 240 Query: 241 IVAAASQAELMEYFRYVPDQVELTQLAAQWLAPAPEPAERASAGAHAEPAQDPIDAVMAE 300 IVAAASQAELMEYFRYVPDQVELTQLAAQWLAPAPE AERASAGAHAEPAQDPIDAVMAE Sbjct: 241 IVAAASQAELMEYFRYVPDQVELTQLAAQWLAPAPESAERASAGAHAEPAQDPIDAVMAE 300 Query: 301 IFAIQRQILQSEANMGWQDGRTQAVASTLMNCCRSIGNLELIGELEIARQQSLQLRSCDP 360 IFAIQRQILQSEANMGWQDGRTQAVASTLMNCCRSIGNLELIGELEIARQQSLQLRSCDP Sbjct: 301 IFAIQRQILQSEANMGWQDGRTQAVASTLMNCCRSIGNLELIGELEIARQQSLQLRSCDP 360 Query: 361 LLAWLNANPLPLQRGPAVPAASVESAQAEAVAVEM-----RQESLPQPEALSRQESPQVQ 415 LLAWLNANPLPLQRGP ASV SA+A A A E RQESLPQPEALSRQE PQVQ Sbjct: 361 LLAWLNANPLPLQRGPVASVASVASAEAGAEAAEAVAVEVRQESLPQPEALSRQEPPQVQ 420 Query: 416 LQPPAPPLRESLPEESGRAGRRAEESLSGNKSVKVEQVKIDRLMNLIGEMVVSKNALPYL 475 P LRESLPEESGRAGRRAEESLSGNKSVKVEQVKIDRLMNLIGEMVVSKNALPYL Sbjct: 421 PPAPP--LRESLPEESGRAGRRAEESLSGNKSVKVEQVKIDRLMNLIGEMVVSKNALPYL 478 Query: 476 AQRAEDQFGVRELAREIKAQYAVINRIAEEMQDAIMQVRMMPVSVVFQRFPRLVRDISRK 535 AQRAEDQFGVRELAREIKAQYAVINRIAEEMQDAIMQVRMMPVSVVFQRFPRLVRDISRK Sbjct: 479 AQRAEDQFGVRELAREIKAQYAVINRIAEEMQDAIMQVRMMPVSVVFQRFPRLVRDISRK 538 Query: 536 LGKEVQLVLVGEETEADKNIIEALADPLIHIVRNSLDHGLEGPEQRRASGKPALGSLTIS 595 LGKEVQLVLVGEETEADKNIIEALADPLIHIVRNSLDHGLEGPEQRRASGKPALGSLTIS Sbjct: 539 LGKEVQLVLVGEETEADKNIIEALADPLIHIVRNSLDHGLEGPEQRRASGKPALGSLTIS 598 Query: 596 AAHEGDQVVIVIEDDGKGIDPALIKRKAYEKGLIDEAQLERISDQDAVNLVFSSGFSTAE 655 AAHEGDQVVIVIEDDGKGIDPALIKRKAYEKGLIDEAQLERISDQDAVNLVFSSGFSTAE Sbjct: 599 AAHEGDQVVIVIEDDGKGIDPALIKRKAYEKGLIDEAQLERISDQDAVNLVFSSGFSTAE 658 Query: 656 VVSDLSGRGVGMDVVRNAVEKVNGSLALHSVSGKGTRLQISLPLSMAVTKVMIIESDGQL 715 VVSDLSGRGVGMDVVRNAVEKVNGSLALHSVSGKGTRLQISLPLSMAVTKVMIIESDGQL Sbjct: 659 VVSDLSGRGVGMDVVRNAVEKVNGSLALHSVSGKGTRLQISLPLSMAVTKVMIIESDGQL 718 Query: 716 FGVPMEHVMETVRVPRSSLHFANSSAKMVLRGHILGLKGINQILGIAAEQKTNADGEVAV 775 FGVPMEHVMETVRVPRSSLHFANSSAKMVLRGHILGLKGINQILGIAAEQKTNADGEVAV Sbjct: 719 FGVPMEHVMETVRVPRSSLHFANSSAKMVLRGHILGLKGINQILGIAAEQKTNADGEVAV 778 Query: 776 LVVRVGGQSVGLVIDDFRETVDVIQKPLLGILGGLSLYSGSALMGDGSVLMVLNTREMF 834 LVVRVGGQSVGLVIDDFRETVDVIQKPLLGILGGLSLYSGSALMGDGSVLMVLNTREMF Sbjct: 779 LVVRVGGQSVGLVIDDFRETVDVIQKPLLGILGGLSLYSGSALMGDGSVLMVLNTREMF 837 >gi|300309957|ref|YP_003774049.1| chemotaxis histidine kinase [Herbaspirillum seropedicae SmR1] Length = 725 Score = 255 bits (652), Expect = 1e-68, Method: Compositional matrix adjust. Identities = 145/397 (36%), Positives = 243/397 (61%), Gaps = 28/397 (7%) Query: 436 RRAEESLSGNKSVKVEQVKIDRLMNLIGEMVVSKNALPYLAQRAEDQFGVRELAREIKAQ 495 RRA+E K +K+E K+D+L++L+GE+V++ + +A+R +D + + Sbjct: 326 RRAQE----QKFIKIEVSKLDQLIDLVGELVIAGASARLIARRRKDS--------QFEEA 373 Query: 496 YAVINRIAEEMQDAIMQVRMMPVSVVFQRFPRLVRDISRKLGKEVQLVLVGEETEADKNI 555 I+ + E+++DA + +RM+ ++ VFQRFPR+VRDI+R LGK++ LV+ G +TE DK++ Sbjct: 374 TQAIDTLMEQIRDAALTLRMVQINEVFQRFPRIVRDIARDLGKDIDLVMTGSDTELDKSM 433 Query: 556 IEALADPLIHIVRNSLDHGLEGPEQRRASGKPALGSLTISAAHEGDQVVIVIEDDGKGID 615 +E LADPL+HIVRN++DHG+E +R A+GKPA SL ++A HE VVI + DDG+G+D Sbjct: 434 VERLADPLMHIVRNAIDHGIEPAAERLAAGKPAKASLQLNAIHESGSVVIEVMDDGRGMD 493 Query: 616 PALIKRKAYEKGLID-EAQLERISDQDAVNLVFSSGFSTAEVVSDLSGRGVGMDVVRNAV 674 I KA +GL +A+L SD + VF GFSTA V++LSGRGVGMDVVR + Sbjct: 494 REAILAKAVAQGLASPDAEL---SDAEIYRFVFEPGFSTARQVTELSGRGVGMDVVRRNI 550 Query: 675 EKVNGSLALHSVSGKGTRLQISLPLSMAVTKVMIIESDGQLFGVPMEHVMETVRVP--RS 732 E ++G +++ + G+GT +++ LPL++A+ + +F +P++ V+E + +P + Sbjct: 551 EALHGEVSIDTGRGRGTVVRLRLPLTLAIISGFQVVVGNAVFVIPLDMVVECIDMPPQQP 610 Query: 733 SLHFANSSAKMVLRGHILGLKGINQILGIAAEQKTNADGEVAVLVVRVGGQSVGLVIDDF 792 H N +R L + ++ + A ++ G ++++V+ G GL++D Sbjct: 611 GSHIVN------VRDEPLPFVPLRELFDLPARER----GRNSLVIVQYGPLRAGLLVDGL 660 Query: 793 RETVDVIQKPLLGILGGLSLYSGSALMGDGSVLMVLN 829 + KPL + G + SGS ++GDG V ++L+ Sbjct: 661 LGECQAVIKPLGRLFGKVKGLSGSTILGDGRVALILD 697 Score = 58.9 bits (141), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 72/282 (25%), Positives = 115/282 (40%), Gaps = 31/282 (10%) Query: 5 RDQFIIETRDLLQGISEKLMQLEDAPEHVELLESLFRMVHTLKGNSGLFDVPEMTRVLHA 64 R + E R+LL + L+Q E + + ++FR HT+KG++GLF + + H Sbjct: 7 RHAMVQEARELLVAMEAALLQTEMEGPSRDSIHAIFRAAHTIKGSAGLFAFDSIVQFTHH 66 Query: 65 CEDLMDAVRRRQVAYSEVLADRLLDAMDFVAMLCDGLEMEAEQADPHAQEAVQIASSLRQ 124 E +++ VR ++A L LL D++ L D +E E +P+A+ + + L Sbjct: 67 LEHVLERVREERLALDSHLMSLLLQCSDYIGELVDAIERGQEAEEPNAERRAALLAEL-- 124 Query: 125 LIGEHAGVESAVGDLARIGDAQDRGAAIVAAPAAGQALPLRAIPEPLRRQAFAQAQAGGE 184 AV + D G A V P A AL A E A A A Sbjct: 125 ---------DAVAAF-----SVDHGPASVPVPLA--ALRGAAGSE----SATADQAATRV 164 Query: 185 LHW-IAYRPSRECFFQGDDP---FHRAREVPGFIWGRIDAPAGWPPLAELNAYDCVLHFQ 240 +W ++ + + G DP H R + G I + + P A ++A C L + Sbjct: 165 PYWHLSLQLNENVLRDGLDPLSFLHYLRSL-GRIVALVTVESAIPEAAAMDAESCYLGME 223 Query: 241 IVAAASQA--ELMEYFRYVPDQVELTQLAAQWLAPAPEPAER 280 + A+ L F +V D + L + AP AER Sbjct: 224 VCLASDSTLQTLENVFEFVRDDSRIDILPPR--APLSAYAER 263 >gi|300312286|ref|YP_003776378.1| signal-transducing histidine kinase CheA protein [Herbaspirillum seropedicae SmR1] Length = 697 Score = 236 bits (602), Expect = 5e-63, Method: Compositional matrix adjust. Identities = 137/388 (35%), Positives = 229/388 (59%), Gaps = 15/388 (3%) Query: 445 NKSVKVEQVKIDRLMNLIGEMVVSKNALPYLAQRAE--DQFGVRELAREIKAQYAVINRI 502 + S++V K+D+L+NL+GE+V+++ + QR D L + ++R Sbjct: 304 SSSIRVSVEKVDQLINLVGELVITQ---AMIEQRVGKLDPMTHEHLLNSVSQ----LSRN 356 Query: 503 AEEMQDAIMQVRMMPVSVVFQRFPRLVRDISRKLGKEVQLVLVGEETEADKNIIEALADP 562 ++Q+++M +RMMP+ VF RFPR+VRD++ KLGK+V+ V G TE DK +IE + DP Sbjct: 357 TRDLQESVMSIRMMPMDYVFSRFPRMVRDLAHKLGKQVEFVTHGAATELDKGLIERIVDP 416 Query: 563 LIHIVRNSLDHGLEGPEQRRASGKPALGSLTISAAHEGDQVVIVIEDDGKGIDPALIKRK 622 L H+VRNS+DHG+E P+ RRASGK G+L++SA H+G ++I + DDG G++ I K Sbjct: 417 LTHLVRNSIDHGIELPDARRASGKSEAGTLSLSAEHQGGNIIIEVTDDGGGLNREKILAK 476 Query: 623 AYEKGLIDEAQLERISDQDAVNLVFSSGFSTAEVVSDLSGRGVGMDVVRNAVEKVNGSLA 682 A ++G+ + +SD + L+F GFSTAE V+D+SGRGVGMDVV+ + + G++ Sbjct: 477 AKQQGM---PVTDNLSDAEVWQLIFEPGFSTAEQVTDVSGRGVGMDVVKRNILAMGGAVD 533 Query: 683 LHSVSGKGTRLQISLPLSMAVTKVMIIESDGQLFGVPMEHVMETVR-VPRSSLHFANSSA 741 + S G GT + ISLPL++A+ M I +++ +P+ +V+E+++ + + + Sbjct: 534 IRSSKGFGTTISISLPLTLAILDGMSIRIGEEVYILPLGYVVESLQPLAQDIKEISGQGQ 593 Query: 742 KMVLRGHILGLKGINQILGIAAEQKTNADGEVAVLVVRVGGQSVGLVIDDFRETVDVIQK 801 + +RG L L + QI I + G V +L G+ L +DD ++ K Sbjct: 594 VLRVRGEYLPLIPLYQIFNIEPSFTDPSQGLVVIL--ESDGKKAALFVDDLIGQQQIVVK 651 Query: 802 PLLGILGGLSLYSGSALMGDGSVLMVLN 829 L ++ SG+ ++GDG V ++++ Sbjct: 652 NLESNYRKVAGISGATILGDGGVALIVD 679 >gi|300312396|ref|YP_003776488.1| chemotaxis CheA protein [Herbaspirillum seropedicae SmR1] Length = 638 Score = 232 bits (591), Expect = 1e-61, Method: Compositional matrix adjust. Identities = 147/400 (36%), Positives = 233/400 (58%), Gaps = 23/400 (5%) Query: 436 RRAEESLSGNK--SVKVEQVKIDRLMNLIGEMVVSKNALPYLAQRAEDQFGVRELAREIK 493 RRA +S G K S++++ K+D L+ + GE V + N L ++ + QF A + Sbjct: 237 RRASDSPGGAKEESIRIDAGKLDALLEVAGESVQAANQAAVLLEKLQ-QFKFEGTAAALM 295 Query: 494 AQYAVI----NRIAEEMQDAIMQVRMMPVSVVFQRFPRLVRDISRKLGKEVQLVLVGEET 549 + A +R + E+Q A + RM PV +FQ+FPRLVR++++ LGK+V LV+ G ET Sbjct: 296 STLAETLGRASRYSSELQRATLSTRMQPVGRLFQKFPRLVRELAKDLGKDVDLVITGAET 355 Query: 550 EADKNIIEALADPLIHIVRNSLDHGLEGPEQRRASGKPALGSLTISAAHEGDQVVIVIED 609 E D+ ++++L DPL+H++RNSLDHG+E R A+GKP +++++A G V+I + D Sbjct: 356 EVDRVVVDSLYDPLVHMLRNSLDHGIE--VDRVAAGKPTKATISLNAWQAGSSVMIEVFD 413 Query: 610 DGKGIDPALIKRKAYEKGLIDEAQLERISDQDAVNLVFSSGFSTAEVVSDLSGRGVGMDV 669 DGKG+DP +++ KA KGLI+E Q ++ +A+ LVF GFST EV S +SGRGVGMDV Sbjct: 414 DGKGMDPDMLRSKALAKGLINEHQ--ALTADEALQLVFLPGFSTKEVASSVSGRGVGMDV 471 Query: 670 VRNAVEKVNGSLALHSVSGKGTRLQISLPLSMAVTKVMIIESDGQLFGVPMEHVMETVRV 729 V+ AVEK G++ + S GKGT+ I LP+ +++ M++ G +PM V V + Sbjct: 472 VKTAVEKHRGTIRIESTIGKGTKFSIRLPIELSIIPTMLVRCAGAPLALPMAVVERVVEL 531 Query: 730 PRSSLHFANSSAKMVLRGHILGLKGINQILGIAAEQKTNADGEVAVLVVRVGGQSVGLVI 789 P +F VLR L +N + G+ + N E +V+ V + L + Sbjct: 532 PE---NFDEVGGAPVLRDQGRPLP-VNSLAGVLGYEPGN---ERVGIVMAVPHPYI-LGV 583 Query: 790 DDFRETVDVIQKPLLGI-LGGLSLYSGSALMGDGSVLMVL 828 + T D++ KPL I G+ +G+A +G +++V+ Sbjct: 584 ESVEGTADLVIKPLTAIHTAGI---TGTARSAEGELVLVV 620 Score = 67.0 bits (162), Expect = 7e-12, Method: Compositional matrix adjust. Identities = 32/93 (34%), Positives = 54/93 (58%), Gaps = 1/93 (1%) Query: 3 LLRDQFIIETRDLLQGISEKLMQLEDAPEHVELLESLFRMVHTLKGNSGLFDVPEMTRVL 62 +L+D F++E +L + E L+ LE P+ +E+L ++FR HT+KG +G ++P M Sbjct: 5 MLKD-FVVEALELATNVEEHLLTLERHPDDLEILNAVFRAFHTIKGGAGFVNLPAMVSAC 63 Query: 63 HACEDLMDAVRRRQVAYSEVLADRLLDAMDFVA 95 H E+L DA+R + + + + L A FVA Sbjct: 64 HLTENLFDALRTGKAPVTPLAIEAALQASGFVA 96 >gi|300312322|ref|YP_003776414.1| two component hybrid sensor histidine kinase/response regulator protein [Herbaspirillum seropedicae SmR1] Length = 775 Score = 189 bits (479), Expect = 1e-48, Method: Compositional matrix adjust. Identities = 125/434 (28%), Positives = 227/434 (52%), Gaps = 44/434 (10%) Query: 443 SGNKSVKVEQVKIDRLMNLIGEMVV-SKNALPY------------------------LAQ 477 SG ++V+V +DRL+ L GE +V S+ P+ LA Sbjct: 193 SGARAVRVSADSLDRLLGLTGESLVESRQLKPFSASMLRMKRTQREAMQALDLLQQKLAT 252 Query: 478 RAEDQFG------VRELARE----IKAQYAVIN-------RIAEEMQDAIMQVRMMPVSV 520 D+F +R L ++ + Q + ++ +++ + D + RM P + Sbjct: 253 TQLDEFAQTAFAELRTLMQQNQQLLGEQLSALDAFDRRSVNLSQRLYDEALACRMRPFAD 312 Query: 521 VFQRFPRLVRDISRKLGKEVQLVLVGEETEADKNIIEALADPLIHIVRNSLDHGLEGPEQ 580 R+VRD+ LGK V+L +VG T+ D++I+E L P+ H++RN++DHG+E Q Sbjct: 313 GTAGLARMVRDVGNTLGKSVRLDIVGNTTQIDRDILEKLEAPIGHLLRNAVDHGIESSAQ 372 Query: 581 RRASGKPALGSLTISAAHEGDQVVIVIEDDGKGIDPALIKRKAYEKGLIDEAQLERISDQ 640 RRA+GKP G + + A H ++I + DDG GID ++ +GL ++ ER+S+ Sbjct: 373 RRAAGKPEQGVIRLEARHSAGMLLIEVADDGAGIDIEQVRSAVVARGLSNQETAERLSEA 432 Query: 641 DAVNLVFSSGFSTAEVVSDLSGRGVGMDVVRNAVEKVNGSLALHSVSGKGTRLQISLPLS 700 + + + FS + V+++SGRGVG+DVV + +++V G++ + + +G+G+R + LPL+ Sbjct: 433 ELLEFLLLPSFSLRDTVTEISGRGVGLDVVADMLKQVRGTIRITTQAGQGSRFLMQLPLT 492 Query: 701 MAVTKVMIIESDGQLFGVPMEHVMETVRVPRSSLHFANSSAKMVLRGHILGLKGINQILG 760 ++V + ++++ G+ + P+ +V T+R+P +L G +GL +Q+L Sbjct: 493 LSVIRSLLVDIAGEPYAFPLAYVNRTLRLPAEALQTLEGYQHFSHGGRQVGLVSAHQVL- 551 Query: 761 IAAEQKTNADGEVAVLVVRVGGQSVGLVIDDFRETVDVIQKPLLGILGGLSLYSGSALMG 820 Q + DG V V+V+ + GL +D F ++ +PL LG ++ ALM Sbjct: 552 -QKGQWRSRDGSVCVVVIGEQEHTYGLAVDAFLGERMLVVQPLDPRLGKIADVLAGALME 610 Query: 821 DGSVLMVLNTREMF 834 DG+ L++L+ +M Sbjct: 611 DGTPLLILDVADML 624 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.134 0.377 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,084,498 Number of extensions: 80569 Number of successful extensions: 372 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 360 Number of HSP's successfully gapped: 9 Length of query: 834 Length of database: 1,619,870 Length adjustment: 93 Effective length of query: 741 Effective length of database: 1,179,515 Effective search space: 874020615 Effective search space used: 874020615 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 135 (56.6 bits)