BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0029.0229.p (280 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311031|ref|YP_003775123.1| chemotaxis protein methyltransf... 578 e-166 gi|300311336|ref|YP_003775428.1| chemotaxis protein methyltransf... 136 1e-33 gi|300309960|ref|YP_003774052.1| chemotaxis protein methyltransf... 135 3e-33 gi|300312324|ref|YP_003776416.1| methyltransferase of chemotaxis... 79 5e-16 gi|300312676|ref|YP_003776768.1| protein-glutamate methyltransfe... 73 2e-14 >gi|300311031|ref|YP_003775123.1| chemotaxis protein methyltransferase [Herbaspirillum seropedicae SmR1] Length = 280 Score = 578 bits (1490), Expect = e-166, Method: Compositional matrix adjust. Identities = 278/280 (99%), Positives = 279/280 (99%) Query: 1 MNETVISEADFLKFREYFYRKTGISFEATKRYFVDKRLIERIEATGSGSFRHYFSLLRFE 60 MNETVISEADFLKFREYFYRKTGISFEATKRYFVDKRLIERIEATGSGSFRHYFSLLRFE Sbjct: 1 MNETVISEADFLKFREYFYRKTGISFEATKRYFVDKRLIERIEATGSGSFRHYFSLLRFE 60 Query: 61 ADGKELQLLTNLMTVNETYFLREEYQFQCLVNSMLPEITGNRRSTAPIRIWCIPCSSGEE 120 ADGKELQLLTNLMTVNETYFLREEYQFQCLVNSMLPEIT NRRSTAPIRIWCIPCSSGEE Sbjct: 61 ADGKELQLLTNLMTVNETYFLREEYQFQCLVNSMLPEITRNRRSTAPIRIWCIPCSSGEE 120 Query: 121 AYTLAMYLMERWPGIDQWDVEIISSDIDTGVLRKAQLGRYSARSVQHVPADWMSKYFLRQ 180 AYTLAMYLMERWPGIDQWDVEIISSDIDTGVLRKAQLGRYSARSVQHVPADWMSKYFLRQ Sbjct: 121 AYTLAMYLMERWPGIDQWDVEIISSDIDTGVLRKAQLGRYSARSVQHVPADWMSKYFLRQ 180 Query: 181 GDEYQICDALRGAVEFTRVNLAKSEDTKAYRNFDVIFCRNLLIYFDDVSRKEAAETFYEA 240 GDEYQICDALRGAVEFTRVNLAKS+DTKAYRNFDVIFCRNLLIYFDDVSRKEAAETFYEA Sbjct: 181 GDEYQICDALRGAVEFTRVNLAKSQDTKAYRNFDVIFCRNLLIYFDDVSRKEAAETFYEA 240 Query: 241 LNLNGFICLGHSESMSRISSLYRVRKFPEAIVYQKTAEAQ 280 LNLNGFICLGHSESMSRISSLYRVRKFPEAIVYQKTAEAQ Sbjct: 241 LNLNGFICLGHSESMSRISSLYRVRKFPEAIVYQKTAEAQ 280 >gi|300311336|ref|YP_003775428.1| chemotaxis protein methyltransferase [Herbaspirillum seropedicae SmR1] Length = 290 Score = 136 bits (343), Expect = 1e-33, Method: Compositional matrix adjust. Identities = 81/260 (31%), Positives = 131/260 (50%), Gaps = 13/260 (5%) Query: 10 DFLKFREYFYRKTGISFEATKRYFVDKRLIERIEATGSGSFRHYFSLLRFEADGKELQLL 69 DF + R Y++ GI+ +K+ V RL R+ ATG SF+ Y +L D +E + Sbjct: 22 DFERVRGLIYQRAGIALADSKQEMVYSRLARRLRATGITSFQQYLDMLERTHDSEEWEAF 81 Query: 70 TNLMTVNETYFLREEYQFQCLVNSMLPEITGNRRSTAPIRIWCIPCSSGEEAYTLAMYLM 129 TN +T N T F RE + F L + + + P IWC S+GEE YT+AM Sbjct: 82 TNALTTNLTSFFREAHHFPILADFL-------KTLKEPATIWCSAASTGEEPYTIAMTAC 134 Query: 130 ERWPGIDQWDVEIISSDIDTGVLRKAQLGRYSARSVQHVPADWMSKYFLRQGDEYQ---- 185 E + G V+I+++DIDT VL G Y ++ + + + K+FLR ++ Sbjct: 135 EAY-GSLTPPVQIVATDIDTNVLATGSNGVYPMERIEKMSPERVKKFFLRGKGSHEGYVR 193 Query: 186 ICDALRGAVEFTRVNLAKSEDTKAYRNFDVIFCRNLLIYFDDVSRKEAAETFYEALNLNG 245 + LR + F ++NL ++ FDVIFCRN++IYFD ++ + F + + Sbjct: 194 VRPELRKLITFKQLNLL-ADGWPLSGQFDVIFCRNVMIYFDKPTQSKILSRFVPLMKPHA 252 Query: 246 FICLGHSESMSRISSLYRVR 265 + GHSE+ +S +++ Sbjct: 253 LLFAGHSENFLYVSDALKLK 272 >gi|300309960|ref|YP_003774052.1| chemotaxis protein methyltransferase [Herbaspirillum seropedicae SmR1] Length = 277 Score = 135 bits (341), Expect = 3e-33, Method: Compositional matrix adjust. Identities = 85/254 (33%), Positives = 129/254 (50%), Gaps = 12/254 (4%) Query: 6 ISEADFLKFREYFYRKTGISFEATKRYFVDKRLIERIEATGSGSFRHYFSLLRFEADGKE 65 +S+ D L F+ F ++ G+ +K+ + RL R+ G Y +L+ EA +E Sbjct: 13 VSDHDMLLFQRLFKQQIGLHLPLSKKALLQSRLAGRLNELGLRHLGQYHALISSEAGAQE 72 Query: 66 LQLLTNLMTVNETYFLREEYQFQCLVNSMLPEITGNRRSTAPIRIWCIPCSSGEEAYTLA 125 Q +L+T NETYF RE F +V LP++ +RIW ++GEEAYTLA Sbjct: 73 RQRAIDLITTNETYFFREPGHFDFVVGQWLPQLA----QQPQLRIWSAASATGEEAYTLA 128 Query: 126 MYLMERWPGIDQWDVEIISSDIDTGVLRKAQLGRYSARSVQHVPADWMSKYFLRQGDEYQ 185 M L PG W V +SDI T VLR A+ Y Q++P ++ +Y L EY+ Sbjct: 129 MLLDHHRPGAS-WSV--FASDISTRVLRFARRALYPMVRGQNIPRAYLRRYCLMGVGEYE 185 Query: 186 ----ICDALRGAVEFTRVNLAKSEDTKAYRNFDVIFCRNLLIYFDDVSRKEAAETFYEAL 241 + LR V+F + NL + D +D+IF RN++IYFD ++ + L Sbjct: 186 GHFLVQADLRAKVQFAQRNLTRL-DEHDDGQYDLIFLRNVIIYFDCETKLQVLRDVIARL 244 Query: 242 NLNGFICLGHSESM 255 G++ +GHSES+ Sbjct: 245 RPGGYLVVGHSESL 258 >gi|300312324|ref|YP_003776416.1| methyltransferase of chemotaxis proteins protein [Herbaspirillum seropedicae SmR1] Length = 433 Score = 79.0 bits (193), Expect = 5e-16, Method: Compositional matrix adjust. Identities = 57/189 (30%), Positives = 92/189 (48%), Gaps = 6/189 (3%) Query: 67 QLLTNLMTVNETYFLREEYQFQCLVNSMLPEITGNRRSTAPIRIWCIPCSSGEEAYTLAM 126 Q L L V ET+F R+ L + L + A IRI +PCS+GEE Y+LAM Sbjct: 58 QALIELAVVPETWFFRDREAI--LAVARLASDKLAAQPAAVIRILSLPCSTGEEPYSLAM 115 Query: 127 YLMERWPGIDQWDVEIISSDIDTGVLRKAQLGRYSARSVQHVPADWMSKYF-LRQGDEYQ 185 L++ G+ I + DI T L A G Y S + + ++F L QG +++ Sbjct: 116 ALLDA--GLTPGQFVIDAYDIRTRSLEIAAAGLYGRNSFRGQELAFRDRHFDLEQG-QWR 172 Query: 186 ICDALRGAVEFTRVNLAKSEDTKAYRNFDVIFCRNLLIYFDDVSRKEAAETFYEALNLNG 245 + + +R V F NL + + + +D IFCRN+LIYF+ +++ + + + Sbjct: 173 LHERIRSQVRFAPGNLLAPDFLRHSQPYDFIFCRNVLIYFERDVQRQVVQLLASVMKPDA 232 Query: 246 FICLGHSES 254 I +G +E Sbjct: 233 LIFVGPAEG 241 >gi|300312676|ref|YP_003776768.1| protein-glutamate methyltransferase chemotaxis response regulator protein [Herbaspirillum seropedicae SmR1] Length = 283 Score = 73.2 bits (178), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 64/213 (30%), Positives = 100/213 (46%), Gaps = 23/213 (10%) Query: 58 RFEADGKELQLLTNLMTVNETYFLREEYQFQCLVNSMLPEITGNRRSTAPIRIWCIPCSS 117 R D LL +TV + R+ F L ++P + R+ ++IW CSS Sbjct: 63 RVLHDAAVFPLLMEYLTVQVSEMFRDPSYFLALREKVVPLL----RTYPSLKIWVAGCSS 118 Query: 118 GEEAYTLAMYLMERWPGIDQWDVEIISSDIDTGVLRKAQLGRYSARSVQHVPADWMSKYF 177 GEE Y+LA+ L R G+ + + I ++DI+ L+KA+ G Y + + Sbjct: 119 GEEVYSLAILL--REEGLLERSI-IYATDINQSALKKAEAGVYDLERIAGFTNNHQ---- 171 Query: 178 LRQGDEYQICD---ALRGAVEF---TRVNLAKSEDTKA----YRNFDVIFCRNLLIYFDD 227 + G + D A GAV F R N+ S+ + A + ++ CRN+LIYF+ Sbjct: 172 -KSGARTSLSDYYTAAYGAVVFDKSLRKNVVFSDHSLATDSVFAEMHLVSCRNVLIYFNR 230 Query: 228 VSRKEAAETFYEALNLNGFICLGHSESMSRISS 260 ++ A F E+L GF+ LG ES+ R SS Sbjct: 231 QLQERALGLFRESLVRKGFLGLGSKESL-RFSS 262 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.137 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 754,429 Number of extensions: 27894 Number of successful extensions: 64 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 54 Number of HSP's successfully gapped: 5 Length of query: 280 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 196 Effective length of database: 1,222,130 Effective search space: 239537480 Effective search space used: 239537480 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 130 (54.7 bits)