BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0029.0227.p (347 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311029|ref|YP_003775121.1| protein-glutamate methylesteras... 677 0.0 gi|300311338|ref|YP_003775430.1| chemotaxis-specific methylester... 207 7e-55 gi|300309961|ref|YP_003774053.1| chemotaxis-specific methylester... 163 2e-41 gi|300312321|ref|YP_003776413.1| protein-glutamate methylesteras... 144 8e-36 gi|300312675|ref|YP_003776767.1| protein-glutamate methylesteras... 63 4e-11 >gi|300311029|ref|YP_003775121.1| protein-glutamate methylesterase [Herbaspirillum seropedicae SmR1] Length = 353 Score = 677 bits (1746), Expect = 0.0, Method: Compositional matrix adjust. Identities = 341/349 (97%), Positives = 342/349 (97%), Gaps = 2/349 (0%) Query: 1 LIIDDSALMRRQLMQVFEQAGGFDICLGRNGREAVELNRSFAPDVVTLDINMPEMDGLSA 60 LIIDDSALMRRQLMQVFEQAGGFDICLGRNGREAVELNRSFAPDVVTLDINMPEMDGLSA Sbjct: 5 LIIDDSALMRRQLMQVFEQAGGFDICLGRNGREAVELNRSFAPDVVTLDINMPEMDGLSA 64 Query: 61 LSLMMLDRPVPVVMVSSLTEQGALATFEAMNLGAVDYVAKPSGTISLSLQEIEQDLLAKV 120 LSLMMLDRPVPVVMVSSLTEQGALATFEAMNLGAVDYVAKPSGTISLSL EIEQDLLAKV Sbjct: 65 LSLMMLDRPVPVVMVSSLTEQGALATFEAMNLGAVDYVAKPSGTISLSLHEIEQDLLAKV 124 Query: 121 RVAAKARIRSRAP--ELARVREPLAAPVAEAARRRRPASDGLVLIGVSTGGPSTLEEILP 178 RVAAKARIRSRAP ELAR REPLAAPVAEAARR RPASDGLVLIGVSTGGPSTLEEILP Sbjct: 125 RVAAKARIRSRAPAPELARAREPLAAPVAEAARRGRPASDGLVLIGVSTGGPSTLEEILP 184 Query: 179 ALPASFPWPILVAQHMPAAFTRSFAERMDKLCALNVLEVAAPTVVQGGHVYIARGGADML 238 ALPASFPWPILVAQHMPAAFTRSFAERMDKLCALNVLEVAAPTVVQGGHVYIARGGADML Sbjct: 185 ALPASFPWPILVAQHMPAAFTRSFAERMDKLCALNVLEVAAPTVVQGGHVYIARGGADML 244 Query: 239 VAKRGSYLSVLPKPESPAHLWHPSVELLARSALQSMPARQLIGVMLTGMGNDGAAAFAEI 298 VAKRGS+LSVLPKPESP HLWHPSVELLARSALQSM ARQLIGVMLTGMGNDGAAAFAEI Sbjct: 245 VAKRGSHLSVLPKPESPTHLWHPSVELLARSALQSMAARQLIGVMLTGMGNDGAAAFAEI 304 Query: 299 CGDGGRTIAESESSCVVFGMPKDLIDRGGASVVLPASKIATQLRVWLEQ 347 CGDGGRTIAESESSCVVFGMPKDLIDRGGASVVLPASKIATQLRVWLEQ Sbjct: 305 CGDGGRTIAESESSCVVFGMPKDLIDRGGASVVLPASKIATQLRVWLEQ 353 >gi|300311338|ref|YP_003775430.1| chemotaxis-specific methylesterase [Herbaspirillum seropedicae SmR1] Length = 359 Score = 207 bits (528), Expect = 7e-55, Method: Compositional matrix adjust. Identities = 139/361 (38%), Positives = 207/361 (57%), Gaps = 29/361 (8%) Query: 1 LIIDDSALMRRQLMQVFEQAGGFDIC-LGRNGREAVELNRSFAPDVVTLDINMPEMDGLS 59 LI+DDSAL+R + ++ ++ + + A EL + PDV+TLD+ MP MDGL Sbjct: 6 LIVDDSALIRSVMREIIGSQPDMEVVGVAPDPIIARELIKQTNPDVLTLDVEMPRMDGLD 65 Query: 60 ALSLMMLDRPVPVVMVSSLTEQGALATFEAMNLGAVDYVAKPSGTISLSLQEIEQDLLAK 119 L +M RP+PVVMVSSLTE+G+ T A+ LGA+D+V KP +I +QE + K Sbjct: 66 FLEKLMRLRPMPVVMVSSLTERGSEITLRALELGAIDFVTKPKISIQSGMQEYADMIADK 125 Query: 120 VRVAAKARIRSR------------APELARVREPLAAPVAEAARRRRPASDGLVLIGVST 167 +R AAKAR+R+R + L ++R PL +S+ L++IG ST Sbjct: 126 IRAAAKARVRARPARSAEQAAGGGSEALPQIRNPLT------------SSEKLIIIGAST 173 Query: 168 GGPSTLEEILPALPASFPWPILVAQHMPAAFTRSFAERMDKLCALNVLEVAAPTVVQGGH 227 GG ++E L +P+ P IL+ QHMP FTRSFA+R++ LC ++V+E A V GH Sbjct: 174 GGTEAIKEFLIRMPSDCPG-ILITQHMPEGFTRSFAQRLNNLCKISVVESAGNERVLPGH 232 Query: 228 VYIARGGADMLVAKRG-SYLSVLPKPESPAHLWHPSVELLARSALQSMPARQLIGVMLTG 286 YIA G + + + + G +Y++ L + P + PSV++L +SA + + +GV+LTG Sbjct: 233 AYIAPGHSHLKLVRSGANYMTQLDQ-GPPVNRHRPSVDVLFQSA-ATCAGKNAVGVILTG 290 Query: 287 MGNDGAAAFAEICGDGGRTIAESESSCVVFGMPKDLIDRGGASVVLPASKIATQLRVWLE 346 MG DGA E+ G A+ E+SCVVFGMP++ I G A VL + ++ WL Sbjct: 291 MGKDGAQGMLEMRNAGAYNFAQDEASCVVFGMPREAIAVGAAHEVLALQAMPGRVLAWLA 350 Query: 347 Q 347 + Sbjct: 351 E 351 >gi|300309961|ref|YP_003774053.1| chemotaxis-specific methylesterase [Herbaspirillum seropedicae SmR1] Length = 381 Score = 163 bits (412), Expect = 2e-41, Method: Compositional matrix adjust. Identities = 121/324 (37%), Positives = 164/324 (50%), Gaps = 32/324 (9%) Query: 43 PDVVTLDINMPEMDGLSALSLMMLDRPVPVVMVSSLTEQGALATFEAMNLGAVDYVAKPS 102 PDV+ LDI MP MDG+S L +M RP PV+M S+ EA+ GAV +A+P Sbjct: 53 PDVIVLDIKMPRMDGVSFLRQIMATRPTPVIMCSAHVGPDNALAAEALAAGAVSVIARPQ 112 Query: 103 GTISLSLQEIEQDLLAKVRVAAKARIRSRAPEL----------ARVREPLAAPVAEAARR 152 + LQ+ ++L + +R A +AR RAP+ A RE L A + Sbjct: 113 LGVRDFLQQAVEELASAIRAAMQAREGQRAPKAPARLGLSPVKAGAREMLHDAAWGAVLQ 172 Query: 153 R----------------RPASDGLVLIGVSTGGPSTLEEILPALPASFPWPILVAQHMPA 196 R RPA+ +V IG STGGP LE +L L P I+V QHMP Sbjct: 173 RYTADAVLQPPGPEDGLRPATARIVAIGASTGGPQALERVLGKLSQRCPG-IVVVQHMPQ 231 Query: 197 AFTRSFAERMDKLCALNVLEVAAPTVVQGGHVYIARGGADMLVAKRG--SYLSVLPKPES 254 FTRSFAER+ ++ V E + G V IA GG ++V + G Y+ L P Sbjct: 232 RFTRSFAERLHRISGAEVKEAEHLDRILPGRVLIAPGGRHLVVRREGLQYYVETLDGPLV 291 Query: 255 PAHLWHPSVELLARSALQSMPARQLIGVMLTGMGNDGAAAFAEICGDGGRTIAESESSCV 314 H PSV++L RS L +G+++TGMG+DGA E+ GG T A+ E+S V Sbjct: 292 SRH--KPSVDVLFRS-LAKAAGSNAVGIIMTGMGDDGARGMRELADCGGATYAQDEASSV 348 Query: 315 VFGMPKDLIDRGGASVVLPASKIA 338 VFGMPK+ I +GG V+ I+ Sbjct: 349 VFGMPKEAIQQGGVGDVITLEHIS 372 >gi|300312321|ref|YP_003776413.1| protein-glutamate methylesterase [Herbaspirillum seropedicae SmR1] Length = 342 Score = 144 bits (364), Expect = 8e-36, Method: Compositional matrix adjust. Identities = 107/344 (31%), Positives = 177/344 (51%), Gaps = 13/344 (3%) Query: 2 IIDDSALMRRQLMQVFEQAGGFD-ICLGRNGREAVELNRSFAPDVVTLDINMPEMDGLSA 60 I++D+ +M L +V ++G + I + R+G +AV++ PDVV +D+ MP++DG A Sbjct: 5 IVNDTPMMVEVLRRVIAESGRHELIWIARDGEQAVQMCAWQLPDVVLMDLLMPKVDGAEA 64 Query: 61 LSLMMLDRPVPVVMVSSLTEQGALATFEAMNLGAVDYVAKPSGTISLSLQEIEQDLLAKV 120 +M P P+++V+S + A +EAM GA+D P+ I + + L+ K+ Sbjct: 65 TRRIMEATPCPILVVTSDMDTSADKIYEAMGYGALDATQTPA-LIGAAGKRDAAALIEKI 123 Query: 121 RVAAKARIRSRAPELAR--VREPLAAPVAEAARRRRPASDGLVLIGVSTGGPSTLEEILP 178 + +PE+ VR A +A A R + LV +G S GGP+ L +L Sbjct: 124 D-----NLGRLSPEMVSPPVRGGRAETLAPAQPARADSLPPLVAVGASAGGPAALATLLK 178 Query: 179 ALPASFPWPILVAQHMPAAFTRSFAERMDKLCALNVLEVAAPTVVQGGHVYIARGGADML 238 A+P F +++ QH+ AAF A+ + + AL V Q G V +A L Sbjct: 179 AIPQGFAGAVVIVQHIDAAFAPGMAQWLQQQSALKVGLAQEGQAPQRGAVLLAASNRH-L 237 Query: 239 VAKRGSYLSVLPKPESPAHLWHPSVELLARSALQSMPARQLIGVMLTGMGNDGAAAFAEI 298 V K G L + +P ++ PSV++ +S ++ P + + G++LTGMG DGAA + Sbjct: 238 VVKEGGRLGYVEG--APNDVYRPSVDVFFQSIVRHWPGK-VAGLLLTGMGRDGAAGLKAL 294 Query: 299 CGDGGRTIAESESSCVVFGMPKDLIDRGGASVVLPASKIATQLR 342 G T+A+ + SC V+GMPK + A VLP + +A +L+ Sbjct: 295 RDSGAHTMAQDQKSCAVYGMPKAAVALNAAVEVLPVTGMAARLQ 338 >gi|300312675|ref|YP_003776767.1| protein-glutamate methylesterase [Herbaspirillum seropedicae SmR1] Length = 211 Score = 62.8 bits (151), Expect = 4e-11, Method: Compositional matrix adjust. Identities = 55/186 (29%), Positives = 82/186 (44%), Gaps = 5/186 (2%) Query: 161 VLIGVSTGGPSTLEEILPALPASFPWPILVAQHMPAAFTRSFAERMDKLCALNVLEVAAP 220 ++IG S GG L EILPAL +V H+P A+ C V+E Sbjct: 17 IVIGASAGGVEALSEILPALRRGAAVATMVVLHLPRERPSLLAQIFADRCRQPVVEAQDK 76 Query: 221 TVVQGGHVYIARGGADMLV--AKRGSYLSVLPKPESPAHLWHPSVELLARSALQSMPARQ 278 Q G ++ A +LV ++ G L++ + + P++++L SA R Sbjct: 77 EPAQAGVIHFAPPDYHLLVDRSEDGPILAM--SNDELVNYSRPAIDVLFESAADVYGPR- 133 Query: 279 LIGVMLTGMGNDGAAAFAEICGDGGRTIAESESSCVVFGMPKDLIDRGGASVVLPASKIA 338 L+G++LTG +DGAA I GG T+ +S V MP + G VL +IA Sbjct: 134 LLGILLTGGNHDGAAGMQAIARAGGVTVVQSPQQAQVPYMPAQALAMGNIDYVLTLQQIA 193 Query: 339 TQLRVW 344 LR Sbjct: 194 DLLRTL 199 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.134 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,042,329 Number of extensions: 42989 Number of successful extensions: 259 Number of sequences better than 1.0e-08: 8 Number of HSP's gapped: 271 Number of HSP's successfully gapped: 8 Length of query: 347 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 261 Effective length of database: 1,212,660 Effective search space: 316504260 Effective search space used: 316504260 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)