BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0029.0226.p (516 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311028|ref|YP_003775120.1| chemotaxis signal transduction ... 1003 0.0 gi|300312285|ref|YP_003776377.1| CheW chemotaxis protein [Herbas... 80 5e-16 gi|300309959|ref|YP_003774051.1| positive regulator of CheA prot... 77 4e-15 >gi|300311028|ref|YP_003775120.1| chemotaxis signal transduction protein [Herbaspirillum seropedicae SmR1] Length = 516 Score = 1003 bits (2594), Expect = 0.0, Method: Compositional matrix adjust. Identities = 510/516 (98%), Positives = 511/516 (99%) Query: 1 MTTDIVTLTETSEPDRPSSQERIATRQFVTFVAGDEVFAVEMAPVQEIIRSPDVVRVPLA 60 MTTDIVTLTETS+PDRPSSQERIATRQFVTFVAGDEVFAVEMAPVQEIIRSPDVVRVPLA Sbjct: 1 MTTDIVTLTETSDPDRPSSQERIATRQFVTFVAGDEVFAVEMAPVQEIIRSPDVVRVPLA 60 Query: 61 PSTLDGLANLRGKILPIVSLRRIFGFPERDKDDATRAVVINVGQPLGFVVDRVASVLDVE 120 P TLDGLANLRGKILPIVSLRRIFGFPERDKDDATRAVVINVGQPLGFVVDRVASVLDVE Sbjct: 61 PPTLDGLANLRGKILPIVSLRRIFGFPERDKDDATRAVVINVGQPLGFVVDRVASVLDVE 120 Query: 121 ADKIEAPEGLQSTIDTELLAGLLKNVGGYPVIMVLDFEKLVSREFSQIAASARNDVRLHA 180 ADKIEAPEGLQSTIDTELLAGLLKNVGGYPVIMVLDFEKLVSREFSQIAASARNDVRLHA Sbjct: 121 ADKIEAPEGLQSTIDTELLAGLLKNVGGYPVIMVLDFEKLVSREFSQIAASARNDVRLHA 180 Query: 181 GLEVHNDDAEAIRNDELQLVSFRLSGQEYAIDIADVQEIVQVPSGIVRLPHSAEHVIGVM 240 GLEVHND AEA RNDELQLVSFRLSGQEYAIDIADVQEIVQVPSGIVRLPHSAEHVIGVM Sbjct: 181 GLEVHNDGAEATRNDELQLVSFRLSGQEYAIDIADVQEIVQVPSGIVRLPHSAEHVIGVM 240 Query: 241 ALRSRLLMLVDLRRMFGLPAQELDDKCRIVVLAHGASSIGLAVDSVSEVLRVPKGLVDPL 300 ALRSRLLMLVDLRRMFGLPAQELDDKCRIVVLAHGASSIGLAVDSVSEVLRVPKGLVDPL Sbjct: 241 ALRSRLLMLVDLRRMFGLPAQELDDKCRIVVLAHGASSIGLAVDSVSEVLRVPKGLVDPL 300 Query: 301 PVALAKSDDAPEIACVCRLDEGRRLVSVITSKNLLDHSALKDAFNIMSEDHSDDAQPGEE 360 PVALAKSDDAPEIACVCRLDEGRRLVSVITSKNLLDHSALKDAFNIMSEDHSD AQPGEE Sbjct: 301 PVALAKSDDAPEIACVCRLDEGRRLVSVITSKNLLDHSALKDAFNIMSEDHSDHAQPGEE 360 Query: 361 IDTAIDDDEQMVIFRLDKEEFGVPIHSVQEIVRVPDELVHVPGAPAFVEGVINLRGSVLP 420 IDTAIDDDEQMVIFRLDKEEFGVPIHSVQEIVRVPDELVHVPGAPAFVEGVINLRGSVLP Sbjct: 361 IDTAIDDDEQMVIFRLDKEEFGVPIHSVQEIVRVPDELVHVPGAPAFVEGVINLRGSVLP 420 Query: 421 VIDLRARLGLPAIERSDGQRIMVFVIADVRTGFIVDQVAEVLRIAKDAIEPAPRFSTQQS 480 VIDLRARLGLPAIERSDGQRIMVFVIADVRTGFIVDQVAEVLRIAKDAIEPAPRFSTQQS Sbjct: 421 VIDLRARLGLPAIERSDGQRIMVFVIADVRTGFIVDQVAEVLRIAKDAIEPAPRFSTQQS 480 Query: 481 ALLARMANMEKQGRMVQLLEPAQLINADEMAGLASL 516 ALLARMANMEKQGRMVQLLEPAQLINADEM GLASL Sbjct: 481 ALLARMANMEKQGRMVQLLEPAQLINADEMTGLASL 516 >gi|300312285|ref|YP_003776377.1| CheW chemotaxis protein [Herbaspirillum seropedicae SmR1] Length = 168 Score = 79.7 bits (195), Expect = 5e-16, Method: Compositional matrix adjust. Identities = 47/155 (30%), Positives = 88/155 (56%), Gaps = 7/155 (4%) Query: 358 GEEIDTAIDDDEQMVIFRLDKEEFGVPIHSVQEIVRVPDELVHVPGAPAFVEGVINLRGS 417 GE+ D+ + + + F L KEE+G+ I VQEI R + + + +P F++GV+NLRG Sbjct: 13 GEKTDS---NGNEFLAFTLGKEEYGIDILKVQEI-RGYEAVTRIANSPDFIKGVVNLRGI 68 Query: 418 VLPVIDLRARLGLPAIERSDGQRIMVFVIADVRTGFIVDQVAEVLRIAKDAIEPAPRFST 477 ++P++D+R + L +++ I G +VD V++V+ + ++ I+PAP T Sbjct: 69 IVPIVDMRIKFQLGEPTYDQFTVVIILNIGGRVVGMVVDSVSDVITLTQEQIKPAPEMGT 128 Query: 478 Q-QSALLARMANMEKQGRMVQLLEPAQLINADEMA 511 S L + +E+ RM+ L++ +L+++ EM Sbjct: 129 TFDSDYLIGLGTLEQ--RMLILVDIDKLMSSAEMG 161 Score = 70.9 bits (172), Expect = 3e-13, Method: Compositional matrix adjust. Identities = 42/119 (35%), Positives = 70/119 (58%), Gaps = 3/119 (2%) Query: 26 RQFVTFVAGDEVFAVEMAPVQEIIRSPDVVRVPLAPSTLDGLANLRGKILPIVSLRRIFG 85 +F+ F G E + +++ VQEI V R+ +P + G+ NLRG I+PIV +R F Sbjct: 21 NEFLAFTLGKEEYGIDILKVQEIRGYEAVTRIANSPDFIKGVVNLRGIIVPIVDMRIKFQ 80 Query: 86 FPERDKDDATRAVVINV-GQPLGFVVDRVASVLDVEADKIE-APEGLQSTIDTELLAGL 142 E D T +++N+ G+ +G VVD V+ V+ + ++I+ APE + +T D++ L GL Sbjct: 81 LGEPTYDQFTVVIILNIGGRVVGMVVDSVSDVITLTQEQIKPAPE-MGTTFDSDYLIGL 138 Score = 60.8 bits (146), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 34/104 (32%), Positives = 59/104 (56%), Gaps = 1/104 (0%) Query: 198 QLVSFRLSGQEYAIDIADVQEIVQVPSGIVRLPHSAEHVIGVMALRSRLLMLVDLRRMFG 257 + ++F L +EY IDI VQEI + + R+ +S + + GV+ LR ++ +VD+R F Sbjct: 22 EFLAFTLGKEEYGIDILKVQEI-RGYEAVTRIANSPDFIKGVVNLRGIIVPIVDMRIKFQ 80 Query: 258 LPAQELDDKCRIVVLAHGASSIGLAVDSVSEVLRVPKGLVDPLP 301 L D +++L G +G+ VDSVS+V+ + + + P P Sbjct: 81 LGEPTYDQFTVVIILNIGGRVVGMVVDSVSDVITLTQEQIKPAP 124 >gi|300309959|ref|YP_003774051.1| positive regulator of CheA protein activity protein [Herbaspirillum seropedicae SmR1] Length = 194 Score = 76.6 bits (187), Expect = 4e-15, Method: Compositional matrix adjust. Identities = 55/158 (34%), Positives = 84/158 (53%), Gaps = 14/158 (8%) Query: 367 DDEQMVIFRLDKEEFGVPIHSVQEIVRVPDELVHVPGAPAFVEGVINLRGSVLPVIDLRA 426 + Q + F + E F +PI S++EI+ L VP PAF+ GVINLRGSV+PVIDL Sbjct: 21 ESRQFLTFLVGTESFAMPIASIREIIEF-SGLTEVPLMPAFLRGVINLRGSVVPVIDLSV 79 Query: 427 RLG-LPAIERSDGQRIMVFVI------ADVRTGFIVDQVAEVLRIAKDAIEPAPRFSTQQ 479 R G P + +R + ++ + G +VD V+ VL I D IEP P F Sbjct: 80 RFGRAPTVV---AKRTCIIIMELQQDEQQLLLGVMVDAVSAVLSIGVDQIEPRPSFGAGI 136 Query: 480 SA-LLARMANMEKQGRMVQLLEPAQLINADEMAGLASL 516 A + M N+ + R + +L+ ++++ DE+A L + Sbjct: 137 RADFVDAMINVNE--RFLVVLDVPRVLSVDELASLLGM 172 Score = 65.1 bits (157), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 42/117 (35%), Positives = 65/117 (55%), Gaps = 9/117 (7%) Query: 16 RPSSQERIATRQFVTFVAGDEVFAVEMAPVQEIIRSPDVVRVPLAPSTLDGLANLRGKIL 75 R + +E + +RQF+TF+ G E FA+ +A ++EII + VPL P+ L G+ NLRG ++ Sbjct: 13 RRAQEEVVESRQFLTFLVGTESFAMPIASIREIIEFSGLTEVPLMPAFLRGVINLRGSVV 72 Query: 76 PIVSLRRIFGFPERDKDDATRAVVI-------NVGQPLGFVVDRVASVLDVEADKIE 125 P++ L FG A R +I LG +VD V++VL + D+IE Sbjct: 73 PVIDLSVRFG--RAPTVVAKRTCIIIMELQQDEQQLLLGVMVDAVSAVLSIGVDQIE 127 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.137 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,569,820 Number of extensions: 68173 Number of successful extensions: 225 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 207 Number of HSP's successfully gapped: 6 Length of query: 516 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 427 Effective length of database: 1,198,455 Effective search space: 511740285 Effective search space used: 511740285 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)