BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0029.0225.p (643 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311027|ref|YP_003775119.1| methyl-accepting chemotaxis pro... 1198 0.0 gi|300310584|ref|YP_003774676.1| methyl-accepting chemotaxis tra... 86 1e-17 gi|300309796|ref|YP_003773888.1| methyl-accepting chemotaxis pro... 82 1e-16 gi|300311037|ref|YP_003775129.1| methyl-accepting chemotaxis tra... 81 2e-16 gi|300310169|ref|YP_003774261.1| methyl-accepting chemotaxis tra... 81 3e-16 >gi|300311027|ref|YP_003775119.1| methyl-accepting chemotaxis protein [Herbaspirillum seropedicae SmR1] Length = 643 Score = 1198 bits (3100), Expect = 0.0, Method: Compositional matrix adjust. Identities = 638/643 (99%), Positives = 641/643 (99%) Query: 1 VALVKKNIPSLSAASFHDNDANRKGGVSVREAEAQRKRARTLAKQQQAAERIAAATGELS 60 +ALVKKNIPSLSAASFHDNDANRKGGVSVREAEAQRKRARTLAKQQQAAERIAAATGELS Sbjct: 1 MALVKKNIPSLSAASFHDNDANRKGGVSVREAEAQRKRARTLAKQQQAAERIAAATGELS 60 Query: 61 SGINEAASAAEQLRRAANQISAGAEEASGAAQESTSAFQLVTESIGIQLQSAEVSESKVN 120 SGINEAASAAEQLRRAANQISAGAEEASGAAQESTSAF LVTESIGIQLQSAEVSESKVN Sbjct: 61 SGINEAASAAEQLRRAANQISAGAEEASGAAQESTSAFHLVTESIGIQLQSAEVSESKVN 120 Query: 121 ACQLVITSTSSDVANLIANVTVAGQRQQGSVAMVSELEAQASNIGDIVKAVARIADQTNL 180 ACQLVITSTSSDVANLIANVTVAGQRQQGSVAMVSELEAQASNIGDIVKAVARIADQTNL Sbjct: 121 ACQLVITSTSSDVANLIANVTVAGQRQQGSVAMVSELEAQASNIGDIVKAVARIADQTNL 180 Query: 181 LALNAAIEAARAGKHGKGFAVVADEVRTLAETSERSAKQIQDLVGQIQQDVKSIAEGINN 240 LALNAAIEAARAGKHGKGFAVVADEVRTLAETSERSAKQIQDLVGQIQQDVKSIAEGINN Sbjct: 181 LALNAAIEAARAGKHGKGFAVVADEVRTLAETSERSAKQIQDLVGQIQQDVKSIAEGINN 240 Query: 241 SARVLGAEVENGKVITAQLERIGVDFREIARGVVEISAGAKQSDMAAQQAMKGSQDIAAA 300 SARVLGAEVENGKVITAQLERIGVDFREIARGVVEISAGAKQSD AAQQAMKGSQDIAAA Sbjct: 241 SARVLGAEVENGKVITAQLERIGVDFREIARGVVEISAGAKQSDTAAQQAMKGSQDIAAA 300 Query: 301 ALQQAAAAEETAKTVSEQSQALAEAEQAAQALAEVAEELKNSTDVAKSAEVVASSAEQLS 360 ALQQAAAAEETAKTVSEQSQALAEAEQAAQALAEVAEELKNSTDVAKSAEVVASSAEQLS Sbjct: 301 ALQQAAAAEETAKTVSEQSQALAEAEQAAQALAEVAEELKNSTDVAKSAEVVASSAEQLS 360 Query: 361 SAVEEINRASAQIMGALEEISKGAQVAAAASEQSSTAIGQIEKGLEVAQQRATVSGEKVK 420 SAVEEINRASAQIMGALEEISKGAQVAAAASE+SSTAIGQIEKG+EVAQQRATVSGEKVK Sbjct: 361 SAVEEINRASAQIMGALEEISKGAQVAAAASEESSTAIGQIEKGVEVAQQRATVSGEKVK 420 Query: 421 GIQSLLITNKEAVGSLIDGIATSVKATQDSIKQVRDLDQVSRRIDKIVEAITTVSIQTNM 480 GIQSLLITNKEAVGSLIDGIATSVKATQDSIKQVRDLDQVSRRIDKIVEAITTVSIQTNM Sbjct: 421 GIQSLLITNKEAVGSLIDGIATSVKATQDSIKQVRDLDQVSRRIDKIVEAITTVSIQTNM 480 Query: 481 LAVNGSVEAARAGEFGKGFVVVSTDIRNLARDSAENADRIKDLVKAVQDQIALVSADLED 540 LAVNGSVEAARAGEFGKGFVVVSTDIRNLARDSAENADRIKDLVKAVQDQIALVSADLED Sbjct: 481 LAVNGSVEAARAGEFGKGFVVVSTDIRNLARDSAENADRIKDLVKAVQDQIALVSADLED 540 Query: 541 IVKAALSEAEKAKTTTANLAQIEADVAEVERGAAAILAASGEISTAIGQAKKGVDQIASA 600 IVKAALSEAEKAKTTTANLAQIEADVAEVERGAAAILAASGEISTAIGQAKKGVDQIASA Sbjct: 541 IVKAALSEAEKAKTTTANLAQIEADVAEVERGAAAILAASGEISTAIGQAKKGVDQIASA 600 Query: 601 AQEAEKAAGEAAGAAEQQSKGARELAAAIEEISSLADELQSVA 643 AQEAEKAAGEAAGAAEQQSKGARELAAAIEEISSLADELQSVA Sbjct: 601 AQEAEKAAGEAAGAAEQQSKGARELAAAIEEISSLADELQSVA 643 >gi|300310584|ref|YP_003774676.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 596 Score = 85.5 bits (210), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 65/174 (37%), Positives = 102/174 (58%), Gaps = 11/174 (6%) Query: 151 VAMVSELEAQASNIGDIVKAVARIADQTNLLALNAAIEAARAGKHGKGFAVVADEVRTLA 210 V + E+ A ++ I DIV + IA QTN+LALNAA+EAARAG+ G+GFAVVA EVR LA Sbjct: 343 VQTMEEIRASSAKITDIVSIIDGIAFQTNILALNAAVEAARAGEQGRGFAVVATEVRALA 402 Query: 211 ETSERSAKQIQDLVGQIQQDVKSIAEGINNSARVLGAEVENGKVITAQLERIGVDFREIA 270 + S +AK+I++L+ V++ +E + + + + + +T + I E + Sbjct: 403 QRSSSAAKEIKELIMHSVDRVQAGSELVGQAGDTMSDVINAVQRVTDIMSEISAASNEQS 462 Query: 271 RGVVEISAGAKQSDMAAQQAMKGSQDIAAAALQQAAAAEETAKTVSEQSQALAE 324 G+ ++S Q D A QQ AA ++QA+AA AK++ +Q+QAL + Sbjct: 463 AGIDQVSHAVTQMDEATQQ--------NAALVEQASAA---AKSLEQQAQALMQ 505 Score = 67.0 bits (162), Expect = 5e-12, Method: Compositional matrix adjust. Identities = 54/165 (32%), Positives = 85/165 (51%), Gaps = 21/165 (12%) Query: 461 SRRIDKIVEAITTVSIQTNMLAVNGSVEAARAGEFGKGFVVVSTDIRNLARDSAENADRI 520 S +I IV I ++ QTN+LA+N +VEAARAGE G+GF VV+T++R LA+ S+ A I Sbjct: 353 SAKITDIVSIIDGIAFQTNILALNAAVEAARAGEQGRGFAVVATEVRALAQRSSSAAKEI 412 Query: 521 KDLVKAVQDQIALVSADLEDIVKAALSEAEKAKTTTANLAQIEADVAEVERGAAAILAAS 580 K+L+ D++ ++L + +A + N Q D+ Sbjct: 413 KELIMHSVDRVQ-AGSEL-------VGQAGDTMSDVINAVQRVTDIMS------------ 452 Query: 581 GEISTAIGQAKKGVDQIASAAQEAEKAAGEAAGAAEQQSKGAREL 625 EIS A + G+DQ++ A + ++A + A EQ S A+ L Sbjct: 453 -EISAASNEQSAGIDQVSHAVTQMDEATQQNAALVEQASAAAKSL 496 >gi|300309796|ref|YP_003773888.1| methyl-accepting chemotaxis protein [Herbaspirillum seropedicae SmR1] Length = 563 Score = 82.0 bits (201), Expect = 1e-16, Method: Compositional matrix adjust. Identities = 64/181 (35%), Positives = 104/181 (57%), Gaps = 24/181 (13%) Query: 50 ERIAAATGELSSGINEAASAAEQLRRAANQISAGAEEASGAAQESTSAFQLVTESIGIQL 109 +++AA E+ G +A+ ++ R +S E+ +GA +E+ S+ + + ++ Sbjct: 264 DKLAAIVAEVRKGSETITTASSEIARGNLDLSGRTEQQAGALEETASSMEQLNSTVK--- 320 Query: 110 QSAEVSESKVNACQLVITSTSSDVANLIANVTVAGQRQQGSVA-----MVSELEAQASNI 164 Q+AE NA Q ++D+A ++V V G GS+ + + A + + Sbjct: 321 QNAE------NAHQ------ANDLALAASDVAVQG----GSIVSQVVQTMQSINASSQKV 364 Query: 165 GDIVKAVARIADQTNLLALNAAIEAARAGKHGKGFAVVADEVRTLAETSERSAKQIQDLV 224 DIV + IA QTN+LALNAA+EAARAG+ G+GFAVVA EVR+LA+ S +AK+I+ L+ Sbjct: 365 ADIVSVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSASAAKEIKVLI 424 Query: 225 G 225 G Sbjct: 425 G 425 Score = 63.5 bits (153), Expect = 5e-11, Method: Compositional matrix adjust. Identities = 32/74 (43%), Positives = 54/74 (72%) Query: 451 IKQVRDLDQVSRRIDKIVEAITTVSIQTNMLAVNGSVEAARAGEFGKGFVVVSTDIRNLA 510 ++ ++ ++ S+++ IV I ++ QTN+LA+N +VEAARAGE G+GF VV++++R+LA Sbjct: 351 VQTMQSINASSQKVADIVSVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLA 410 Query: 511 RDSAENADRIKDLV 524 + SA A IK L+ Sbjct: 411 QRSASAAKEIKVLI 424 >gi|300311037|ref|YP_003775129.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 522 Score = 81.3 bits (199), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 61/155 (39%), Positives = 96/155 (61%), Gaps = 9/155 (5%) Query: 71 EQLRRAANQISAGAEEASGAAQESTSAFQLVTESIGIQLQSAEVSESKVNACQLVITSTS 130 + + RA ++IS+G + SG + S+ + T S Q+ SA V +S NA Q + Sbjct: 272 DSIERACSEISSGNADLSGRTERQASSLE-ETASAMEQITSA-VRQSADNAIQ------A 323 Query: 131 SDVANLIANVTVAGQRQQGSV-AMVSELEAQASNIGDIVKAVARIADQTNLLALNAAIEA 189 + +A +++ + G + G V A + ++ + I DI+ + IA QTN+LALNAA+EA Sbjct: 324 NQLAGSASSIALKGGQMMGDVVATMEDIHHSSVKIVDIISVIDGIAFQTNILALNAAVEA 383 Query: 190 ARAGKHGKGFAVVADEVRTLAETSERSAKQIQDLV 224 ARAG+ G+GFAVVA EVRTLA+ S +AK+I++L+ Sbjct: 384 ARAGEGGRGFAVVASEVRTLAQRSAAAAKEIKELI 418 Score = 68.2 bits (165), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 35/78 (44%), Positives = 53/78 (67%) Query: 449 DSIKQVRDLDQVSRRIDKIVEAITTVSIQTNMLAVNGSVEAARAGEFGKGFVVVSTDIRN 508 D + + D+ S +I I+ I ++ QTN+LA+N +VEAARAGE G+GF VV++++R Sbjct: 343 DVVATMEDIHHSSVKIVDIISVIDGIAFQTNILALNAAVEAARAGEGGRGFAVVASEVRT 402 Query: 509 LARDSAENADRIKDLVKA 526 LA+ SA A IK+L+ A Sbjct: 403 LAQRSAAAAKEIKELISA 420 >gi|300310169|ref|YP_003774261.1| methyl-accepting chemotaxis transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 657 Score = 80.9 bits (198), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 78/250 (31%), Positives = 135/250 (54%), Gaps = 25/250 (10%) Query: 51 RIAAATGELSSGINEAASA----AEQLRRAANQISAG-------AEEASGAAQESTSAFQ 99 R+ A +S G++E S+ E++ AA++I++G E+ +GA +E+ SA + Sbjct: 391 RLMGAFNGISMGLSEVVSSVRAGTEEINTAASEIASGNLNLSVRTEQQAGALEETASAME 450 Query: 100 LVTESIGIQLQSAEVSESKVNACQLVITSTSSDVANLIANVTVAGQRQQGSVAMVSELEA 159 +T ++ +AE ++ ++S +S+VA+ V GQ V + + + Sbjct: 451 ELTSTVKQNAANAEQADE--------LSSAASEVAS--HGGEVVGQ----VVQTMDAINS 496 Query: 160 QASNIGDIVKAVARIADQTNLLALNAAIEAARAGKHGKGFAVVADEVRTLAETSERSAKQ 219 + I DI+ + IA QTN+LALNAA+EAARAG+ G+GFAVVA EVRTLA+ S +AK+ Sbjct: 497 SSQRIVDIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVATEVRTLAQRSAAAAKE 556 Query: 220 IQDLVGQIQQDVKSIAEGINNSARVLGAEVENGKVITAQLERIGVDFREIARGVVEISAG 279 I++L+ Q V + + + + V + + ++ + I RE + G+ +I+ Sbjct: 557 IKELIDASVQTVAGGGQLVTQAGNTMNDVVSSVQRVSTVISEITTASREQSEGIDQINRA 616 Query: 280 AKQSDMAAQQ 289 Q D + QQ Sbjct: 617 IAQMDESTQQ 626 Score = 67.0 bits (162), Expect = 5e-12, Method: Compositional matrix adjust. Identities = 35/66 (53%), Positives = 49/66 (74%) Query: 461 SRRIDKIVEAITTVSIQTNMLAVNGSVEAARAGEFGKGFVVVSTDIRNLARDSAENADRI 520 S+RI I+ I ++ QTN+LA+N +VEAARAGE G+GF VV+T++R LA+ SA A I Sbjct: 498 SQRIVDIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVATEVRTLAQRSAAAAKEI 557 Query: 521 KDLVKA 526 K+L+ A Sbjct: 558 KELIDA 563 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.305 0.118 0.285 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,144,833 Number of extensions: 35943 Number of successful extensions: 682 Number of sequences better than 1.0e-08: 69 Number of HSP's gapped: 519 Number of HSP's successfully gapped: 138 Length of query: 643 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 552 Effective length of database: 1,188,985 Effective search space: 656319720 Effective search space used: 656319720 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.6 bits) S2: 134 (56.2 bits)