BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0029.0216.p (59 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311019|ref|YP_003775111.1| GAF domain-containing protein [... 122 4e-30 gi|300311023|ref|YP_003775115.1| GGDEF family protein [Herbaspir... 57 3e-10 >gi|300311019|ref|YP_003775111.1| GAF domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 144 Score = 122 bits (307), Expect = 4e-30, Method: Compositional matrix adjust. Identities = 59/59 (100%), Positives = 59/59 (100%) Query: 1 VRCFAGMRLRLNNGVVFGALCVMDTEPRIFRAREIRTFEGLAQIAEDIYRLFLFERQSH 59 VRCFAGMRLRLNNGVVFGALCVMDTEPRIFRAREIRTFEGLAQIAEDIYRLFLFERQSH Sbjct: 86 VRCFAGMRLRLNNGVVFGALCVMDTEPRIFRAREIRTFEGLAQIAEDIYRLFLFERQSH 144 >gi|300311023|ref|YP_003775115.1| GGDEF family protein [Herbaspirillum seropedicae SmR1] Length = 236 Score = 57.0 bits (136), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 28/54 (51%), Positives = 37/54 (68%), Gaps = 1/54 (1%) Query: 2 RCFAGMRLRLNNGVVFGALCVMDTEPRIFRAREIRTFEGLAQIAEDIYRLFLFE 55 R +AG+RL + G FG+LCV+DTEPRI R+ E + E LA +A DI RL + E Sbjct: 182 RFYAGVRL-MYEGKAFGSLCVIDTEPRILRSHEQQALEELAMLASDILRLHILE 234 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.336 0.148 0.449 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 159,080 Number of extensions: 4454 Number of successful extensions: 20 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 20 Number of HSP's successfully gapped: 2 Length of query: 59 Length of database: 1,619,870 Length adjustment: 32 Effective length of query: 27 Effective length of database: 1,468,350 Effective search space: 39645450 Effective search space used: 39645450 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 39 (21.7 bits) S2: 123 (52.0 bits)