BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0029.0215.p (442 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311018|ref|YP_003775110.1| CBS domains containing hemolysi... 897 0.0 gi|300309700|ref|YP_003773792.1| Mg2+/Co2+ transporter transmemb... 114 1e-26 gi|300310418|ref|YP_003774510.1| hemolysin protein [Herbaspirill... 97 2e-21 gi|300310054|ref|YP_003774146.1| Mg2+ and Co2+ transporter prote... 76 7e-15 >gi|300311018|ref|YP_003775110.1| CBS domains containing hemolysin protein [Herbaspirillum seropedicae SmR1] Length = 442 Score = 897 bits (2317), Expect = 0.0, Method: Compositional matrix adjust. Identities = 441/442 (99%), Positives = 441/442 (99%) Query: 1 MSIFTSLLLILLLVVVSAFLSCAEISIAASRKIRLEMMSKEGVHNADRVLSLQAQPGNFF 60 MSIFTSLLLILLLVVVSAFLSCAEISIAASRKIRLEMMSKEGVHNADRVLSLQAQPGNFF Sbjct: 1 MSIFTSLLLILLLVVVSAFLSCAEISIAASRKIRLEMMSKEGVHNADRVLSLQAQPGNFF 60 Query: 61 TVVQIGLNAVAILGGVVGEPALTPHMREIISSFYIGPWLENASFAMSFLVVTAFFILFAD 120 TVVQIGLNAVAILGGVVGEPALTPHMREIISSFYIGPWLENASFAMSFLVVTAFFILFAD Sbjct: 61 TVVQIGLNAVAILGGVVGEPALTPHMREIISSFYIGPWLENASFAMSFLVVTAFFILFAD 120 Query: 121 LMPKRLAMVAPERAAVALVKPMLLLETLFKPLVWFFNSLSKWIFTLFGLPQVRQEVITPN 180 LMPKRLAMVAPERAAVALVKPMLLLETLFKPLVWFFNSLSKWIFTLFGLPQVRQEVITPN Sbjct: 121 LMPKRLAMVAPERAAVALVKPMLLLETLFKPLVWFFNSLSKWIFTLFGLPQVRQEVITPN 180 Query: 181 EIHAMVDAGAQAGVLLTQEHHLIENVFELESRYVPSAMTPRDSIVYFLLTDSEATIRQKI 240 EIHAMVDAGAQAGVLLTQEHHLIENVFELESRYVPSAMTPRDSIVYFLLTDSEATIRQKI Sbjct: 181 EIHAMVDAGAQAGVLLTQEHHLIENVFELESRYVPSAMTPRDSIVYFLLTDSEATIRQKI 240 Query: 241 IDHPHSRFLVCDGHIDAVLGYIDSKDVLKRLLKGEQFSLADKGLVHPPLSIPDSLNLWEV 300 IDHPHSRFLVCDGHIDAVLGYIDSKDVLKRLLKGEQFSLADKGLVHPPLSIPDSLNLWEV Sbjct: 241 IDHPHSRFLVCDGHIDAVLGYIDSKDVLKRLLKGEQFSLADKGLVHPPLSIPDSLNLWEV 300 Query: 301 LERMKAMDEDLAIVVNEYALVVGIISITDVTGVLMGNLLAITEEEQQIVRRDENSWLMDG 360 LERMKAMDEDLAIVVNEYALVVGIIS TDVTGVLMGNLLAITEEEQQIVRRDENSWLMDG Sbjct: 301 LERMKAMDEDLAIVVNEYALVVGIISTTDVTGVLMGNLLAITEEEQQIVRRDENSWLMDG 360 Query: 361 LTPLNDVMSELDIDEYPNPVGYETLSGFIMYMLRKIPKKTDFVLFAEFKFEVVDIEGNRI 420 LTPLNDVMSELDIDEYPNPVGYETLSGFIMYMLRKIPKKTDFVLFAEFKFEVVDIEGNRI Sbjct: 361 LTPLNDVMSELDIDEYPNPVGYETLSGFIMYMLRKIPKKTDFVLFAEFKFEVVDIEGNRI 420 Query: 421 NQLLVTRFKQDKSEKDAAPLRD 442 NQLLVTRFKQDKSEKDAAPLRD Sbjct: 421 NQLLVTRFKQDKSEKDAAPLRD 442 >gi|300309700|ref|YP_003773792.1| Mg2+/Co2+ transporter transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 434 Score = 114 bits (286), Expect = 1e-26, Method: Compositional matrix adjust. Identities = 104/433 (24%), Positives = 205/433 (47%), Gaps = 37/433 (8%) Query: 3 IFTSLLLILLLVVVSAFLSCAEISIAASRKIRLEMMSKEGVHNADRVLSLQAQPGNFFTV 62 I+ +L + +L+++SAF S E ++ A+ + RL ++K+G A +L L + ++ Sbjct: 6 IWVQVLALFILILLSAFFSMTETALMAANRHRLRHLAKQGNRAAITILWLLDRTERLLSL 65 Query: 63 VQIG---LNAVAI-LGGVVGEPALTPHMREIISSFYIGPWLENASFAMSFLVVTAFFILF 118 V I +NA+A L + A H R I ++ V A I+F Sbjct: 66 VLIANTAINALATALVTALAIAAFGNHQRVITAATA---------------CVAALLIIF 110 Query: 119 ADLMPKRLAMVAPERAAVA---LVKPMLLLETLFKPLVWFFNSLSKWIFTLFGL---PQV 172 A+++PK + PER ++ ++KP++ + KPL+WF N I L + + Sbjct: 111 AEIVPKVIGATYPERISLFTGFILKPLM---RVAKPLIWFVNLFVSAILRLLRIKTGARA 167 Query: 173 RQEVITPNEIHAMVDAGAQAGVLLTQEHH-LIENVFELESRYVPSAMTPRDSIVYFLLTD 231 ++ I+P E+ ++V G G + Q+H ++ N+F+LE V MTPR + L Sbjct: 168 AEQRISPEELRSIVLEG---GSFIPQKHKSILLNLFDLEKISVEDVMTPRSQVEALNLAA 224 Query: 232 SEATIRQKIIDHPHSRFLVCDGHIDAVLGYIDSKDVLKRLLKGEQFSLAD-KGLVHPPLS 290 I ++ H++ V +G I+ ++G + + + + E+ S D + L+ P Sbjct: 225 PVDDILHQLATCYHNKLPVFEGEINHIVGILHVRKTVSLFRREEEISHDDIRELLSAPYY 284 Query: 291 IPDSLNLWEVLERMKAMDEDLAIVVNEYALVVGIISITDVTGVLMGNLLAIT--EEEQQI 348 +P +++ L+ + E L +VV+EY V G++++ D+ ++G + + Sbjct: 285 VPVETDVFTQLQYFQENHERLGVVVDEYGEVQGLVTLEDIIEEMIGEFTTSSPGSSGEGF 344 Query: 349 VRRDENSWLMDGLTPLNDVMSELDIDEYPNPVGYETLSGFIMYMLRKIPKKTDFVLFAEF 408 + + L++G T L D+ L + + P G +T++G ++ L+ IP+ + V Sbjct: 345 GWDKDGACLLEGSTTLRDINKRLGL-QLPLD-GPKTINGLLLEYLQDIPEASVAVKMDGC 402 Query: 409 KFEVVDIEGNRIN 421 E+V ++ I Sbjct: 403 VIEIVQVQNQAIR 415 >gi|300310418|ref|YP_003774510.1| hemolysin protein [Herbaspirillum seropedicae SmR1] Length = 440 Score = 97.4 bits (241), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 94/426 (22%), Positives = 188/426 (44%), Gaps = 18/426 (4%) Query: 7 LLLILLLVVVSAFLSCAEISIAASRKIRLEMMSK-EGVHNADRVLSL-QAQPGNFFTVVQ 64 +++ LV ++ F AE S+ R+ R+ ++K +G+ R+L++ Q + + Q Sbjct: 7 IVVAFFLVALNGFFVAAEFSLVKLRQTRIRAIAKTQGMRG--RILAVVHNQLDAYLSACQ 64 Query: 65 IGLNAVAILGGVVGEPALTPHMREIISSFYIG--PWLENASFAMSFLVVTAFFILFADLM 122 +G+ ++ G +GEPA + + S + + SF +F V++ I+ +L Sbjct: 65 LGITLASLGLGWIGEPAFARILEPLFSLAGVTNQELIHGVSFVFAFFVISFLHIVAGELA 124 Query: 123 PKRLAMVAPERAAVALVKPMLLLETLFKPLVWFFNSLSKWIFTLFGL--PQVRQEVITPN 180 PK +A+ +PE+ + P+ PL+W N+ S W+ + GL + + Sbjct: 125 PKSMAIRSPEKLGLWCAMPLYGFYWGMYPLIWVLNASSNWLLRVAGLGAGHGHDAHYSSD 184 Query: 181 EIHAMVDAGAQA---GVLLTQEHHLIENVFELESRYVPSAMTPRDSIVYFLLTDSEATIR 237 E+ ++ AG ++ G E +++ V M P IV S Sbjct: 185 ELKLILRAGNKSGKNGKFTRDEWNVLTQSLNFAELDVADIMRPASEIVALGDDKSLEENL 244 Query: 238 QKIIDHPHSRFLVCDGHIDAVLGYIDSKDVLKRLLKGEQFSLAD-KGLVHPPLSIPDSLN 296 I + +SR+ D VLG + KDV L + + ++A+ K + P I +L Sbjct: 245 DIIYRNRYSRYPYYDAERQQVLGLVHLKDVF--LAQQDGRAIANLKDYLRPVQYISPALP 302 Query: 297 LWEVLERMKAMDEDLAIVVNEYALVVGIISITDVTGVLMGNLL-AITEEEQQIVRRDENS 355 ++L R + A++ + G I++ ++ +L+G + + R+D+ + Sbjct: 303 ALDLLRRFRTGSPHFAVIGKKGQPPAGFITLDNMLSLLVGEIRDEFRHNTGEWTRQDDGT 362 Query: 356 WLMDGLTPLNDVMSELDID-EYPNPVGYETLSGFIMYMLRKIPKKTDFVLFAEFKFEVVD 414 L G P+ + + L ID EY + + +++ G +M L +PK+ + F F + Sbjct: 363 LLGKGSLPIVTLENMLGIDIEYDDSI--DSVGGLVMEKLGDLPKEGQKIDFPAFDVVIKR 420 Query: 415 IEGNRI 420 + G +I Sbjct: 421 MSGPKI 426 >gi|300310054|ref|YP_003774146.1| Mg2+ and Co2+ transporter protein [Herbaspirillum seropedicae SmR1] Length = 292 Score = 75.9 bits (185), Expect = 7e-15, Method: Compositional matrix adjust. Identities = 64/245 (26%), Positives = 115/245 (46%), Gaps = 34/245 (13%) Query: 202 LIENVFELESRYVPSAMTPRDSIVYFLLTDSEATIRQKIIDHPHSRFLVCDGHIDAVLGY 261 +IE VF++ V M PR + ++ T +++ HSRF DG D V+G Sbjct: 56 MIEGVFQVSDLAVRDIMVPRSQMDTIDISKPIETWMPEVLATSHSRFPAVDGERDKVVGI 115 Query: 262 IDSKDVLKRLLKGEQFSLADKGLVHPPLSIPDSLNLWEVLERMKAMDEDLAIVVNEYALV 321 + +KD+L R E F + D ++ P + IP+S L +L +A +A+VV+EY V Sbjct: 116 LLAKDLL-RYYAEESFDVRD--MLRPAIFIPESKRLNVLLRDFRANRNHMAVVVDEYGGV 172 Query: 322 VGIISITDVTGVLMGNLLAITEEEQQIVRRDEN-----------SWLMDGLTPLNDVMSE 370 G+I+I DV ++G++ E+E ++N W + LT Sbjct: 173 AGLITIEDVLEQIVGDI----EDEYDFDEAEDNVLALKDGPHGARWRVKALT-------- 220 Query: 371 LDIDEYPNPVGY-------ETLSGFIMYMLRKIPKKTDFVLFAEFKFEVVDIEGNRINQL 423 +I+++ VG +T+ G + + ++P+K D FEV+ + +++ L Sbjct: 221 -EIEQFNEEVGSRFSDDDADTIGGLVANHVGRVPRKGDEFDIDGLHFEVLRADARQVHVL 279 Query: 424 LVTRF 428 LV++ Sbjct: 280 LVSQL 284 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.324 0.139 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,263,420 Number of extensions: 52358 Number of successful extensions: 139 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 131 Number of HSP's successfully gapped: 4 Length of query: 442 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 354 Effective length of database: 1,203,190 Effective search space: 425929260 Effective search space used: 425929260 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 132 (55.5 bits)