BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0029.0214.p
         (223 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300311017|ref|YP_003775109.1| glutathione S-transferase famil...   447   e-127
gi|300312317|ref|YP_003776409.1| glutathione S-transferase [Herb...    67   9e-13
gi|300311872|ref|YP_003775964.1| glutathione S-transferase famil...    62   5e-11

>gi|300311017|ref|YP_003775109.1| glutathione S-transferase family
           protein [Herbaspirillum seropedicae SmR1]
          Length = 223

 Score =  447 bits (1150), Expect = e-127,   Method: Compositional matrix adjust.
 Identities = 220/223 (98%), Positives = 221/223 (99%)

Query: 1   MITVHHLNNSRSQRVLWLLEELGLDYEIKFYQRDPKTMLAPPELLKVHPLGKSPVITDGD 60
           MITVHHLNNSRSQRVLWLLEELGLDYEIKFYQRDPKTMLAPPELLKVHPLGKSPVITDG+
Sbjct: 1   MITVHHLNNSRSQRVLWLLEELGLDYEIKFYQRDPKTMLAPPELLKVHPLGKSPVITDGE 60

Query: 61  LTVAESGNIIEYLLERYGAHSGLLPAPGTPERLRYRYWLHFAEGSAMPWLVMQLIFAKMP 120
           LTVAESGNIIEYLLERYGAHSGLLPA GTPERLRYRYWLHFAEGSAMPWLVMQLIFAKMP
Sbjct: 61  LTVAESGNIIEYLLERYGAHSGLLPASGTPERLRYRYWLHFAEGSAMPWLVMQLIFAKMP 120

Query: 121 KAVPALIRPIARMLAEGMRSRVVAPQQERQLRFMEDELGQQGWFAGAQFSGADIQMSFPV 180
           KAVP LIRPIARMLAEGMRSRVVAPQQERQLRFMEDELGQQGWFAGAQFSGADIQMSFPV
Sbjct: 121 KAVPTLIRPIARMLAEGMRSRVVAPQQERQLRFMEDELGQQGWFAGAQFSGADIQMSFPV 180

Query: 181 EALRARGGLDGRYPNLLKYLEAIHARPAYQRALEKGGPYELLR 223
           EALRARGGLDGRYPNLLKYLEAIHARPAYQRALEKGGPYELLR
Sbjct: 181 EALRARGGLDGRYPNLLKYLEAIHARPAYQRALEKGGPYELLR 223


>gi|300312317|ref|YP_003776409.1| glutathione S-transferase
           [Herbaspirillum seropedicae SmR1]
          Length = 230

 Score = 67.4 bits (163), Expect = 9e-13,   Method: Compositional matrix adjust.
 Identities = 64/232 (27%), Positives = 91/232 (39%), Gaps = 49/232 (21%)

Query: 1   MITVHHLNNSRSQRVLWLLEELGLDYEIKFYQRDPKTMLAPPELLKVHPLGKSPVITDGD 60
           MI V+        +V  +LEE GL Y              P E LK+ P  K P I D D
Sbjct: 1   MIDVYSWATPNGHKVHIMLEECGLPYTAHAIDIGAGDQFTP-EFLKISPNNKIPAIVDPD 59

Query: 61  ------LTVAESGNIIEYLLERYGAHSGLLPAPGTPERLRYRYWLHFAEGSAMPWLVMQL 114
                 +++ ESG I+ YL  + G   G      T E+     W+ F  G   P      
Sbjct: 60  GPDGEPISLFESGAILIYLAGKTGRFLG----ETTREKYEVLQWVMFQMGGLGP------ 109

Query: 115 IFAKMPKAVPALIRPIARMLAEGMRSRVVAPQQ------------ERQLRFMEDELGQQG 162
                             ML +    R+ AP+Q             R  + M+ +LGQ  
Sbjct: 110 ------------------MLGQAHHFRLYAPEQIEYAVNRYTNEARRLYQVMDKQLGQHA 151

Query: 163 WFAGAQFSGADIQMSFPVEALRARGGLD-GRYPNLLKYLEAIHARPAYQRAL 213
           + AG  ++ ADI   FP        G+D   +PN+ ++ +AI ARPA QR +
Sbjct: 152 YLAGEHYTIADI-ACFPWTRSHKNQGIDLDDFPNVKRWYDAISARPAVQRGV 202


>gi|300311872|ref|YP_003775964.1| glutathione S-transferase family
           protein [Herbaspirillum seropedicae SmR1]
          Length = 235

 Score = 61.6 bits (148), Expect = 5e-11,   Method: Compositional matrix adjust.
 Identities = 67/227 (29%), Positives = 92/227 (40%), Gaps = 32/227 (14%)

Query: 2   ITVHHLNNSRSQRVLWLLEELGLDYEIKFYQRDPKTMLAPPELLKVHPLGKSPVITDGD- 60
           I ++ L      +V  +LEE GL YE      +    ++P E L ++P  K P I D + 
Sbjct: 22  IQLYSLPTPNGVKVSIMLEETGLPYEAHLVSFETNDQMSP-EFLSLNPNNKIPAIIDPNG 80

Query: 61  -----LTVAESGNIIEYLLERYGAHSGLLPAPGTPERLRYRYWLHFAEGSAMPWLVMQLI 115
                L + ESG I+ YL E+ G     LPA     R +   W+ F  G   P       
Sbjct: 81  PGGQPLPLFESGAILLYLAEKTGQ---FLPADAA-ARYQVIQWVMFQMGGVGPMFGQLGF 136

Query: 116 FAKMPKAVPALIRPIARMLAEGMRSRVVAPQQERQLRFMEDELGQQGWFAGAQFSGADIQ 175
           F +         RP  R + E           +R L  M+  L Q  W  G  +S ADI 
Sbjct: 137 FHRFAGKEYEDKRPRDRYVNE----------SKRLLGVMDKRLAQSQWLGGDAYSIADI- 185

Query: 176 MSFP--------VEALRARGGLDGRYPNLLKYLEAIHARPAYQRALE 214
            +FP         EA    G  D  +P++ + LEA  ARPA  R L 
Sbjct: 186 ATFPWIRNLIGFYEAADLVGFQD--FPHVARALEAFVARPAVVRGLN 230


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.322    0.139    0.425 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 789,643
Number of extensions: 34867
Number of successful extensions: 109
Number of sequences better than 1.0e-08: 3
Number of HSP's gapped: 109
Number of HSP's successfully gapped: 3
Length of query: 223
Length of database: 1,619,870
Length adjustment: 82
Effective length of query: 141
Effective length of database: 1,231,600
Effective search space: 173655600
Effective search space used: 173655600
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 129 (54.3 bits)