BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0029.0189.p (373 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310991|ref|YP_003775083.1| branched-chain amino acid ABC t... 749 0.0 gi|300312761|ref|YP_003776853.1| branched-chain amino acid ABC t... 204 1e-53 gi|300309516|ref|YP_003773608.1| branched-chain amino acid ABC t... 171 6e-44 gi|300310948|ref|YP_003775040.1| branched-chain amino acid ABC t... 137 1e-33 gi|300312236|ref|YP_003776328.1| amino acid ABC transporter peri... 114 2e-26 >gi|300310991|ref|YP_003775083.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 373 Score = 749 bits (1935), Expect = 0.0, Method: Compositional matrix adjust. Identities = 372/373 (99%), Positives = 372/373 (99%) Query: 1 MKFASRLTKIALAAATFAAFAGVAHAETVKIAIAGPMTGAVAQYGDMVKAGALTAIEQIN 60 MKFASRLTKIALAAATFAAFAGVAHAETVKIAIAGPMTGAVAQYGDMVKAGALTAIEQIN Sbjct: 1 MKFASRLTKIALAAATFAAFAGVAHAETVKIAIAGPMTGAVAQYGDMVKAGALTAIEQIN 60 Query: 61 AAGGANGNKFEAVMMDDACEPKQAVAVANKIVSQGIKYVIGHVCSGSTIPASDIYENEGV 120 AAGGANGNKFEAVMMDDACEPKQAVAVANKIVSQGIKYVIGHVCSGSTIPASDIYENEGV Sbjct: 61 AAGGANGNKFEAVMMDDACEPKQAVAVANKIVSQGIKYVIGHVCSGSTIPASDIYENEGV 120 Query: 121 VMITPSATAPQLTEAKPHKFIFRTIGRDDQQGPAAARYVIEKLKPKKVAVLHDKQSYGQG 180 VMITPSATAPQLTEAKPHKFIFRTIGRDDQQGPAAARYVIEKLKPKKVAVLHDKQSYGQG Sbjct: 121 VMITPSATAPQLTEAKPHKFIFRTIGRDDQQGPAAARYVIEKLKPKKVAVLHDKQSYGQG 180 Query: 181 VASSVKAALDAAKVPVVVFEGINAGDSDYSAIVTKLKSQGVDFVYFGGYHPEMGLIMRQA 240 VASSVKAALDAAKVPVVVFEGINAGDSDYSAIVTKLKSQGVDFVYFGGYHPEMGLIMRQA Sbjct: 181 VASSVKAALDAAKVPVVVFEGINAGDSDYSAIVTKLKSQGVDFVYFGGYHPEMGLIMRQA 240 Query: 241 REQGIKAVFMGPEGVGNKDITAIAGPASEGMLVTLPADFAADPANAALVKAFAAAKRDPN 300 REQGIKAVFMGPEGVGNKDITAIAGPASEGMLVTLPADFAADPANAALVKAFAAAKRDPN Sbjct: 241 REQGIKAVFMGPEGVGNKDITAIAGPASEGMLVTLPADFAADPANAALVKAFAAAKRDPN 300 Query: 301 GPFQMPAYSGVKIIADAITGAKSTDPEKVAAYIHANSFKTPIGNVEYDKKGDLKSFKFVV 360 GPFQMPAYSGVKIIADAI GAKSTDPEKVAAYIHANSFKTPIGNVEYDKKGDLKSFKFVV Sbjct: 301 GPFQMPAYSGVKIIADAIAGAKSTDPEKVAAYIHANSFKTPIGNVEYDKKGDLKSFKFVV 360 Query: 361 FTWHKDASKTEAK 373 FTWHKDASKTEAK Sbjct: 361 FTWHKDASKTEAK 373 >gi|300312761|ref|YP_003776853.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 380 Score = 204 bits (518), Expect = 1e-53, Method: Compositional matrix adjust. Identities = 139/371 (37%), Positives = 199/371 (53%), Gaps = 11/371 (2%) Query: 1 MKFASRLTKIALAAATFAAFAGVAHA-ETVKIAIAGPMTGAVAQYGDMVKAGALTAIEQI 59 MK S + I L AA AFAG A++ E VKIA GP++G A G + GA A++++ Sbjct: 1 MKLKSAI--IPLTAAIGLAFAGAAYSQEVVKIAHVGPLSGPNAHMGKDNENGARMAVDEL 58 Query: 60 NAAG---GANGNKFEAVMMDDACEPKQAVAVANKIVSQGIKYVIGHVCSGSTIPASDIYE 116 NA G G KFE V DDA +PKQA AVA K+V Q + VIGH+ SG+TIPAS IY Sbjct: 59 NAKGFTIGGKKVKFELVGEDDASDPKQATAVATKLVDQKVAAVIGHLNSGTTIPASKIYS 118 Query: 117 NEGVVMITPSATAPQLTEAKPHKFIFRTIGRDDQQGPAAARYVIEKLKPKKVAVLHDKQS 176 + G+ ++PSAT P+ T+ + K FR + D Q G A +Y ++KL K++AV+ D+ + Sbjct: 119 DAGIPQVSPSATNPKYTQ-QGFKTAFRVVANDAQLGAALGKYAVQKLGTKQIAVIDDRTA 177 Query: 177 YGQGVASSVKAALDAAKVPVVVFEGINAGDSDYSAIVTKLKSQGVDFVYFGGYHPEMGLI 236 YGQGVA AA +V + N +D++AI+T LK + D V+FGG G + Sbjct: 178 YGQGVADEFAKGAKAAGGTIVGTQYTNDKATDFNAILTSLKGKKPDVVFFGGMDAVGGPM 237 Query: 237 MRQAREQGIKAVFMGPEGVGNKDITAIAGPASEGMLVTLPADFAADPANAALVKAFAAAK 296 +RQ ++ GI A FMG +G+ + +AG V D A + F AA Sbjct: 238 LRQMKQLGISAKFMGGDGICTGSLPGLAGDGLGDDQVICAEAGGVDEAGKKGMDDFRAAY 297 Query: 297 RDPNGPFQM----PAYSGVKIIADAITGAKSTDPEKVAAYIHANSFKTPIGNVEYDKKGD 352 + G + AY +ADA+ A S DP+K + S K G + +D KGD Sbjct: 298 KKKFGIDVVYNAAYAYDATMTVADAMAKAGSADPKKYLPELAKISHKGVTGVIAFDAKGD 357 Query: 353 LKSFKFVVFTW 363 +K ++T+ Sbjct: 358 IKDGSLTLYTY 368 >gi|300309516|ref|YP_003773608.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 378 Score = 171 bits (434), Expect = 6e-44, Method: Compositional matrix adjust. Identities = 119/369 (32%), Positives = 186/369 (50%), Gaps = 7/369 (1%) Query: 1 MKFASRLTKIALAAATFAAFAGVAHAETVKIAIAGPMTGAVAQYGDMVKAGALTAIEQIN 60 K S L IA + A AFA + VKI + P+TG A G + G + AIE++N Sbjct: 2 FKRVSGLNIIAASLALIPAFAMAQETQVVKIGFSSPLTGPQASAGKDNQGGLMMAIERLN 61 Query: 61 AAG---GANGNKFEAVMMDDACEPKQAVAVANKIVSQGIKYVIGHVCSGSTIPASDIYEN 117 A G KF+ + DD +PK VAVA K+ QG+K ++G SG TIPAS +Y + Sbjct: 62 AQPITVGGKKIKFDVIAEDDQADPKSGVAVAQKLADQGVKAIVGPYNSGVTIPASRVYND 121 Query: 118 EGVVMITPSATAPQLTEAKPHKFIFRTIGRDDQQGPAAARYVIEKLKPKKVAVLHDKQSY 177 G+V+ T A+ P++T+ + +FR D Q G A Y ++LK K+VAV+ D+ +Y Sbjct: 122 AGIVVAT-VASNPKITQ-QGFATLFRVAASDSQLGGKMALYAAKELKFKRVAVIDDRTAY 179 Query: 178 GQGVASSVKAALDAAKVPVVVFEGINAGDSDYSAIVTKLKSQGVDFVYFGGYHPEMGLIM 237 GQG+A A + VV + N +D++AI+T +K + D V+ GGY P+ G I Sbjct: 180 GQGLAQEFIKVAKANGIDVVSTDFTNDKATDFTAILTSIKGKKPDAVFLGGYAPQGGPIK 239 Query: 238 RQAREQGIKAVFMGPEGVGNKDITAIAGPASEGMLVTLPADFAADPANAALVKAFAAAKR 297 RQ ++ G+ MG +G+ + ++ + G A + D A V + K+ Sbjct: 240 RQMKQLGVDVPLMGGDGICSPEMGRLGGDAIGESVYCTQGGTMLDKAKEGKVFSDEYQKK 299 Query: 298 --DPNGPFQMPAYSGVKIIADAITGAKSTDPEKVAAYIHANSFKTPIGNVEYDKKGDLKS 355 P + + Y G+ +IA A+ A S DP++ + S+K G ++D DLK Sbjct: 300 YNRPAETYAVSFYDGMMLIAQAMKQANSVDPKQFGPALAKISYKGVAGQYDFDANHDLKQ 359 Query: 356 FKFVVFTWH 364 V+ + Sbjct: 360 SPVTVYRFK 368 >gi|300310948|ref|YP_003775040.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 394 Score = 137 bits (345), Expect = 1e-33, Method: Compositional matrix adjust. Identities = 92/338 (27%), Positives = 158/338 (46%), Gaps = 5/338 (1%) Query: 28 TVKIAIAGPMTGAVAQYGDMVKAGALTAIEQINAAG---GANGNKFEAVMMDDACEPKQA 84 T+ I AGP++G G ++ G A+ + N G G + + DD +P A Sbjct: 39 TLAIGFAGPLSGPSGGVGKSMQNGVQLALAEANRRGLRIGGKSWQLRLIAQDDRADPATA 98 Query: 85 VAVANKIVSQGIKYVIGHVCSGSTIPASDIYENEGVVMITPSATAPQLTEAKPHKFIFRT 144 VA +V Q + V+GH SG+++ A+ +Y GV+ +TPSA + +LT A + IFRT Sbjct: 99 EFVARSLVQQQVIAVVGHWTSGTSLAAAPVYHRAGVIQVTPSAMSRRLT-AMSYPRIFRT 157 Query: 145 IGRDDQQGPAAARYVIEKLKPKKVAVLHDKQSYGQGVASSVKAALDAAKVPVVVFEGINA 204 I ++ G AA Y ++KL+ + + + D+ +GQG+A VV ++ Sbjct: 158 IPNNESLGRLAAGYAVDKLETRTIVTIDDRTPFGQGLAEQFARTASERGAQVVGRYSVSD 217 Query: 205 GDSDYSAIVTKLKSQGVDFVYFGGYHPEMGLIMRQAREQGIKAVFMGPEGVGNKDITAIA 264 SD++A + + + D ++FGG + G++ + R + A M G A Sbjct: 218 KTSDFNAALLEARKLQPDLIFFGGLDWQAGVLAKSIRRLKLHARLMASPGTLGLPFLMRA 277 Query: 265 GPASEGMLVTLPADFAAD-PANAALVKAFAAAKRDPNGPFQMPAYSGVKIIADAITGAKS 323 GP + G LV P P + + ++ + + AY + I I A S Sbjct: 278 GPDANGALVLEPGPPQDKMPGWKSFRQRYSENFDSDMDLYAVFAYEAAQAIIQGIRQAGS 337 Query: 324 TDPEKVAAYIHANSFKTPIGNVEYDKKGDLKSFKFVVF 361 DPE++A +H F+ G V ++++GDL F ++ Sbjct: 338 ADPERIAEAMHRLRFEGVSGPVAFNEEGDLLQPSFTMY 375 >gi|300312236|ref|YP_003776328.1| amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 385 Score = 114 bits (284), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 101/328 (30%), Positives = 157/328 (47%), Gaps = 12/328 (3%) Query: 1 MKFASRLTKIALAAATFAAFAGVAHAETVKIAIAGPMTGAVAQYGDMVKAGALTAIEQIN 60 M+F + L I A A + A A +K+ +A +TG A+YG +K G A E+IN Sbjct: 1 MQFNTMLKTIP-ALLIGATLSQAALAADIKLGVAEALTGPAAKYGVAIKNGFTLASEEIN 59 Query: 61 AAGGANGNKFEAVMMDDACEPKQAVAVANKIVSQG-IKYVIGHVCSGSTIPASDIYENEG 119 A GG NG+K V+ D+ + ++A+ V K++ Q + V G S S A I Sbjct: 60 AKGGVNGDKLALVIEDEQGKKEEAINVFKKLIFQDKVLAVFGPTLSNSAFAADPIANAAK 119 Query: 120 VVMITPSATAPQLTEAKPHKFIFRTIGRDDQQGPAAARYVIEKLKPKKVAVLH-DKQSYG 178 VV+ S TA +T P F FR + P + ++ KKVAV++ + ++ Sbjct: 120 VVVFGTSNTADGITAMGP--FTFRNSVMEADVLPVTVKAAVKHFGIKKVAVIYGNDDAFT 177 Query: 179 QGVASSVKAALDAAKVPVVVFEGINAGDSDYSAIVTKLKSQGVDFVYFGGYHPEMGLIMR 238 + KA L+ K+PV E GD D+ A +TK+K+ D + E I+ Sbjct: 178 KSGYDVFKATLEHQKIPVTTTEAYAKGDVDFKAQLTKIKAGNPDAIVCSCLAEEAANIIL 237 Query: 239 QAREQGIKAVFMGPEGVGNKDITAIAGPASEGMLVTLPADFAAD---PANAALVKAFAAA 295 Q R G+K F+G G+ + + IA A + L+ P ++A+ PAN A + A+ A Sbjct: 238 QTRALGMKQPFIGGNGLNSPKLFEIAKDAGDNTLMGSP--WSAENQTPANKAFITAYKAK 295 Query: 296 -KRDPNGPFQMPAYSGVKIIADAITGAK 322 DP+ F AY + I+ADA+ K Sbjct: 296 FGADPD-QFAAQAYDAMYIVADALKNVK 322 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.131 0.371 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,013,632 Number of extensions: 39337 Number of successful extensions: 198 Number of sequences better than 1.0e-08: 13 Number of HSP's gapped: 179 Number of HSP's successfully gapped: 13 Length of query: 373 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 286 Effective length of database: 1,207,925 Effective search space: 345466550 Effective search space used: 345466550 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)