BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0029.0176.p (279 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310979|ref|YP_003775071.1| alkanesulfonates ABC transporte... 541 e-155 gi|300309985|ref|YP_003774077.1| nitrate/sulfonate/bicarbonate A... 186 2e-48 gi|300309716|ref|YP_003773808.1| nitrate/sulfonate/bicarbonate A... 158 4e-40 gi|300311644|ref|YP_003775736.1| taurine ABC transporter permeas... 138 5e-34 gi|300310817|ref|YP_003774909.1| nitrate/sulfonate/bicarbonate A... 115 5e-27 >gi|300310979|ref|YP_003775071.1| alkanesulfonates ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 279 Score = 541 bits (1395), Expect = e-155, Method: Compositional matrix adjust. Identities = 279/279 (100%), Positives = 279/279 (100%) Query: 1 MTSASNPVAGRVAGPQEQAKPKAARKSFQPSHAWIGWLLPLLLLVWWEVAAQAGWLSSRI 60 MTSASNPVAGRVAGPQEQAKPKAARKSFQPSHAWIGWLLPLLLLVWWEVAAQAGWLSSRI Sbjct: 1 MTSASNPVAGRVAGPQEQAKPKAARKSFQPSHAWIGWLLPLLLLVWWEVAAQAGWLSSRI 60 Query: 61 LPEPLAVLKAAWELSASGELWRHLAVSSGRAASGFAVGAGLGLILGLLSGSLRSAELLLD 120 LPEPLAVLKAAWELSASGELWRHLAVSSGRAASGFAVGAGLGLILGLLSGSLRSAELLLD Sbjct: 61 LPEPLAVLKAAWELSASGELWRHLAVSSGRAASGFAVGAGLGLILGLLSGSLRSAELLLD 120 Query: 121 TTLQMVRNIPALALIPLVILWFGIDETAKLFLVSVGVFFPVYLNTFHGIRSVDQGLIEMA 180 TTLQMVRNIPALALIPLVILWFGIDETAKLFLVSVGVFFPVYLNTFHGIRSVDQGLIEMA Sbjct: 121 TTLQMVRNIPALALIPLVILWFGIDETAKLFLVSVGVFFPVYLNTFHGIRSVDQGLIEMA 180 Query: 181 KSYGLSGWPLYRDVILPGALPAILVGLRFALGLVWVLLIVAETISAQAGIGYMTMNAREF 240 KSYGLSGWPLYRDVILPGALPAILVGLRFALGLVWVLLIVAETISAQAGIGYMTMNAREF Sbjct: 181 KSYGLSGWPLYRDVILPGALPAILVGLRFALGLVWVLLIVAETISAQAGIGYMTMNAREF 240 Query: 241 LQTDVVLVGILLYALLGKLADTAARWLERFWLRWHPGYQ 279 LQTDVVLVGILLYALLGKLADTAARWLERFWLRWHPGYQ Sbjct: 241 LQTDVVLVGILLYALLGKLADTAARWLERFWLRWHPGYQ 279 >gi|300309985|ref|YP_003774077.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 278 Score = 186 bits (471), Expect = 2e-48, Method: Compositional matrix adjust. Identities = 114/274 (41%), Positives = 162/274 (59%), Gaps = 9/274 (3%) Query: 2 TSASNPVAGRVAGPQEQAKPKAARKSFQPSHAWIGWLLPLLLLVWWEVAAQAGWLSSRIL 61 TS S R P + A ++P + GW++PL L+ E A+ + +R+L Sbjct: 3 TSTSTARTHRRGWPHDDA--------YRPGR-FTGWIVPLAFLLLVEAGARLELIPARLL 53 Query: 62 PEPLAVLKAAWELSASGELWRHLAVSSGRAASGFAVGAGLGLILGLLSGSLRSAELLLDT 121 P P + W L+ GEL H+A S R A+GF +G+ L + LGLL G R AE LL+ Sbjct: 54 PPPSELAHTLWSLAQRGELLPHIAASLARVAAGFVIGSALAVALGLLVGISRRAEALLEP 113 Query: 122 TLQMVRNIPALALIPLVILWFGIDETAKLFLVSVGVFFPVYLNTFHGIRSVDQGLIEMAK 181 + Q +R IP+LA IPL++LW GIDET K+ L+++G FFPVYLN GIR +D LIE+ Sbjct: 114 SFQALRAIPSLAWIPLLLLWLGIDETPKVVLIAIGAFFPVYLNLVAGIRDIDHKLIEVGT 173 Query: 182 SYGLSGWPLYRDVILPGALPAILVGLRFALGLVWVLLIVAETISAQAGIGYMTMNAREFL 241 + GL+ L ++LP +LP + GLR L L W+ L+ AE I+A G+GY+ + RE Sbjct: 174 TSGLNTQALIGHILLPASLPYLFAGLRGGLSLAWMFLVAAELIAATKGVGYLLSDGRESS 233 Query: 242 QTDVVLVGILLYALLGKLADTAARWLERFWLRWH 275 + D+VL IL+ ALLGKL+D R +E+ L W Sbjct: 234 RPDLVLAAILILALLGKLSDGLLRAVEQKSLGWR 267 >gi|300309716|ref|YP_003773808.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 273 Score = 158 bits (400), Expect = 4e-40, Method: Compositional matrix adjust. Identities = 103/261 (39%), Positives = 152/261 (58%), Gaps = 17/261 (6%) Query: 36 GWLLPLLLLVWWEVAAQAGWLSSRILPEPLAVLKAAWELSA-----------------SG 78 G L+P++++ WE A++AGW++ +ILP P AVL+ E + SG Sbjct: 12 GLLVPIIVIALWEGASRAGWINPQILPSPWAVLRKWVEYATPLKPYDPEAGSWLAWAFSG 71 Query: 79 ELWRHLAVSSGRAASGFAVGAGLGLILGLLSGSLRSAELLLDTTLQMVRNIPALALIPLV 138 EL S R +GF +GAGL L LGLL GS + L++ T+Q++R IP +A IPL Sbjct: 72 ELVMDAIGSLYRVVAGFLIGAGLALPLGLLMGSSQRIYGLMNLTVQVIRPIPPIAYIPLA 131 Query: 139 ILWFGIDETAKLFLVSVGVFFPVYLNTFHGIRSVDQGLIEMAKSYGLSGWPLYRDVILPG 198 ILWFG+ LFL+S+G FFPV +NT G+R VD + A++ G S L+ V+LP Sbjct: 132 ILWFGLGNPPALFLISLGAFFPVLMNTIAGVRQVDSIYLRAARNLGASQSTLFLRVMLPA 191 Query: 199 ALPAILVGLRFALGLVWVLLIVAETISAQAGIGYMTMNAREFLQTDVVLVGILLYALLGK 258 A+P IL G+R +G ++++IVAE I+ G+G+ M ARE+ +D ++ G++ LLG Sbjct: 192 AVPYILSGVRIGIGTAFIVVIVAEMIAVSNGLGFRIMEAREYFWSDKIIAGMITIGLLGL 251 Query: 259 LADTAARWLERFWLRWHPGYQ 279 D L + LRWH G + Sbjct: 252 AIDLGMSRLNNYMLRWHRGLE 272 >gi|300311644|ref|YP_003775736.1| taurine ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 293 Score = 138 bits (347), Expect = 5e-34, Method: Compositional matrix adjust. Identities = 102/266 (38%), Positives = 144/266 (54%), Gaps = 7/266 (2%) Query: 17 EQAKPKAARKSF---QPSHAWIGWLLPLLLLVWWEVAAQAGWLSSRILPEPLAVLKAA-- 71 +Q P +SF + S A I +++L W + AQ GW+ LP P VLK A Sbjct: 25 DQPPPLVKMRSFGIAEGSTARISIAAGIVILALWWLIAQTGWVPHLFLPTPAEVLKVAQS 84 Query: 72 -WELS-ASGELWRHLAVSSGRAASGFAVGAGLGLILGLLSGSLRSAELLLDTTLQMVRNI 129 WE A+ L H+ S R + + LG+ +GLL G R + +LDT ++ + Sbjct: 85 IWEDGYANATLAEHVGASLLRILTAGVIAVLLGIPVGLLMGLNRWVKGVLDTPIEFYWPL 144 Query: 130 PALALIPLVILWFGIDETAKLFLVSVGVFFPVYLNTFHGIRSVDQGLIEMAKSYGLSGWP 189 P LA +PL+I+W GI E +K+ L+++ +F PV L+ G+RS+ Q + A S G + W Sbjct: 145 PPLAYLPLMIIWLGIGEASKITLLTLAMFAPVVLSAQAGVRSLPQERVNAALSLGATRWQ 204 Query: 190 LYRDVILPGALPAILVGLRFALGLVWVLLIVAETISAQAGIGYMTMNAREFLQTDVVLVG 249 L +V+LP ALP IL G+R ALG+ W L+ AE I++ GIGYM M+A FL TD V VG Sbjct: 205 LLTEVVLPSALPEILTGIRIALGVGWSTLVAAELIASNRGIGYMIMSASHFLATDAVFVG 264 Query: 250 ILLYALLGKLADTAARWLERFWLRWH 275 I L A R LER + W Sbjct: 265 IALIATCAFAFSYGMRLLERALVPWK 290 >gi|300310817|ref|YP_003774909.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 256 Score = 115 bits (287), Expect = 5e-27, Method: Compositional matrix adjust. Identities = 75/226 (33%), Positives = 118/226 (52%), Gaps = 2/226 (0%) Query: 44 LVWWEVAAQAGWLSSRILPEPLAVLKAAWELSASGELWRHLAVSSGRAASGFAVGAGLGL 103 LV W++ + S LP AA EL + G L + + SSGR +G+ G +G+ Sbjct: 26 LVLWQLVSLTT--SPLFLPSVSMTWAAALELISDGTLQQSVLASSGRILAGWGAGVVIGV 83 Query: 104 ILGLLSGSLRSAELLLDTTLQMVRNIPALALIPLVILWFGIDETAKLFLVSVGVFFPVYL 163 LG+ G + LLD ++ R IP +A + L ++W G E +K+ L+ F V L Sbjct: 84 PLGIFMGRFETVRQLLDPYIEFFRFIPPIAFVTLAVIWLGPGELSKIALIFYTTVFIVTL 143 Query: 164 NTFHGIRSVDQGLIEMAKSYGLSGWPLYRDVILPGALPAILVGLRFALGLVWVLLIVAET 223 NT G+++V+ + A S G W + VILP +P ++ G R A+G ++ ++ AE Sbjct: 144 NTIAGVQAVNPLRLRAAASLGAGQWKILSTVILPSTVPFMVTGARIAMGNSFLTVVSAEI 203 Query: 224 ISAQAGIGYMTMNAREFLQTDVVLVGILLYALLGKLADTAARWLER 269 +SA+ G+G + AR F +T+ V VGI+ +LG L D R R Sbjct: 204 VSAREGLGALIWTARNFSRTEWVFVGIIALGILGYLFDRVLRVATR 249 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.324 0.139 0.441 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 803,528 Number of extensions: 30627 Number of successful extensions: 241 Number of sequences better than 1.0e-08: 17 Number of HSP's gapped: 233 Number of HSP's successfully gapped: 17 Length of query: 279 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 195 Effective length of database: 1,222,130 Effective search space: 238315350 Effective search space used: 238315350 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 130 (54.7 bits)