BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0029.0175.p (388 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310978|ref|YP_003775070.1| alkanesulfonate monooxygenase [... 784 0.0 gi|300310902|ref|YP_003774994.1| alkanesulfonate monooxygenase [... 478 e-136 gi|300310284|ref|YP_003774376.1| coenzyme F420-dependent N5,N10-... 123 3e-29 >gi|300310978|ref|YP_003775070.1| alkanesulfonate monooxygenase [Herbaspirillum seropedicae SmR1] Length = 388 Score = 784 bits (2025), Expect = 0.0, Method: Compositional matrix adjust. Identities = 388/388 (100%), Positives = 388/388 (100%) Query: 1 MNVFWFIPTHGDSRYLGTSEGARAVSHDYMKQVAVAADTLGYDGVLLPTGRSCEDAWVAA 60 MNVFWFIPTHGDSRYLGTSEGARAVSHDYMKQVAVAADTLGYDGVLLPTGRSCEDAWVAA Sbjct: 1 MNVFWFIPTHGDSRYLGTSEGARAVSHDYMKQVAVAADTLGYDGVLLPTGRSCEDAWVAA 60 Query: 61 ASLIDATRRLKFLVAVRPGLMAPTLAARMAATFDRLSGGRLLINVVTGGDPVEQESDGVF 120 ASLIDATRRLKFLVAVRPGLMAPTLAARMAATFDRLSGGRLLINVVTGGDPVEQESDGVF Sbjct: 61 ASLIDATRRLKFLVAVRPGLMAPTLAARMAATFDRLSGGRLLINVVTGGDPVEQESDGVF 120 Query: 121 GSHAERYEISDEFLKIWRDVLTKTRSGEPTHFQGKHLSVKGAKALYPPVQQPYPPLYFGG 180 GSHAERYEISDEFLKIWRDVLTKTRSGEPTHFQGKHLSVKGAKALYPPVQQPYPPLYFGG Sbjct: 121 GSHAERYEISDEFLKIWRDVLTKTRSGEPTHFQGKHLSVKGAKALYPPVQQPYPPLYFGG 180 Query: 181 SSDEAIDLAAEQVDVYLTWGEPPAAVAEKIARVRERAAKAGRTLRFGIRLHVIVRETNEA 240 SSDEAIDLAAEQVDVYLTWGEPPAAVAEKIARVRERAAKAGRTLRFGIRLHVIVRETNEA Sbjct: 181 SSDEAIDLAAEQVDVYLTWGEPPAAVAEKIARVRERAAKAGRTLRFGIRLHVIVRETNEA 240 Query: 241 AWRAADELISRLDDETIAKAQANFARMDSEGQRRMAALHGGRRDKLEVSPNLWAGVGLVR 300 AWRAADELISRLDDETIAKAQANFARMDSEGQRRMAALHGGRRDKLEVSPNLWAGVGLVR Sbjct: 241 AWRAADELISRLDDETIAKAQANFARMDSEGQRRMAALHGGRRDKLEVSPNLWAGVGLVR 300 Query: 301 GGAGTALVGDGPTVAERLQEYAALGIDTFILSGYPHLEESYRFAELVFPLLPRGQREGGS 360 GGAGTALVGDGPTVAERLQEYAALGIDTFILSGYPHLEESYRFAELVFPLLPRGQREGGS Sbjct: 301 GGAGTALVGDGPTVAERLQEYAALGIDTFILSGYPHLEESYRFAELVFPLLPRGQREGGS 360 Query: 361 GQQLPGPFGEIVGNTELPKAAPVRVSAS 388 GQQLPGPFGEIVGNTELPKAAPVRVSAS Sbjct: 361 GQQLPGPFGEIVGNTELPKAAPVRVSAS 388 >gi|300310902|ref|YP_003774994.1| alkanesulfonate monooxygenase [Herbaspirillum seropedicae SmR1] Length = 375 Score = 478 bits (1229), Expect = e-136, Method: Compositional matrix adjust. Identities = 231/351 (65%), Positives = 278/351 (79%), Gaps = 4/351 (1%) Query: 1 MNVFWFIPTHGDSRYLGTSEGARAVSHDYMKQVAVAADTLGYDGVLLPTGRSCEDAWVAA 60 + +FWF+PT GD+RYLG S+ RA + +YM+Q+AV ++TLGYDG+L+PTG SC D WV A Sbjct: 3 LELFWFLPTSGDTRYLGKSDFGRAPTLEYMRQIAVTSETLGYDGLLIPTGASCLDPWVVA 62 Query: 61 ASLIDATRRLKFLVAVRPGLMAPTLAARMAATFDRLSGGRLLINVVTGGDPVEQESDGVF 120 ASL+ T+R+K LVA+R L P +AR AAT D+ GRLL+NVV GGD E E+DGVF Sbjct: 63 ASLVPVTQRIKLLVALRTSLGGPGPSARQAATLDQALNGRLLLNVVPGGDATELEADGVF 122 Query: 121 GSHAERYEISDEFLKIWRDVLTKTRSGEPTHFQGKHLSVKGAKALYPPVQQPYPPLYFGG 180 H ERYE +DE+L IWR +L GE +++GKH ++ + +P VQQPYPPLYFGG Sbjct: 123 LKHDERYEAADEYLTIWRKLL----QGETVNYEGKHFRIRNGQLFHPAVQQPYPPLYFGG 178 Query: 181 SSDEAIDLAAEQVDVYLTWGEPPAAVAEKIARVRERAAKAGRTLRFGIRLHVIVRETNEA 240 SS A DLAA+ VD YLTWGEPPAAV +KI VRERAA+ GRT+RFG+RLH IVRET Sbjct: 179 SSPAAHDLAAKHVDAYLTWGEPPAAVRQKIDDVRERAARQGRTVRFGVRLHAIVRETTAE 238 Query: 241 AWRAADELISRLDDETIAKAQANFARMDSEGQRRMAALHGGRRDKLEVSPNLWAGVGLVR 300 AW AA+ LIS+L DE IA+AQ N+ARMDS GQ+RMAALHGGRRDKLE++PNLWAGVGLVR Sbjct: 239 AWTAAERLISKLTDEDIARAQQNYARMDSVGQQRMAALHGGRRDKLEIAPNLWAGVGLVR 298 Query: 301 GGAGTALVGDGPTVAERLQEYAALGIDTFILSGYPHLEESYRFAELVFPLL 351 GGAGT+LVGD TVAERLQEYA LG+DTF+LSGYPHLEE+ RFAELVFPLL Sbjct: 299 GGAGTSLVGDATTVAERLQEYADLGVDTFVLSGYPHLEEAIRFAELVFPLL 349 >gi|300310284|ref|YP_003774376.1| coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase [Herbaspirillum seropedicae SmR1] Length = 385 Score = 123 bits (308), Expect = 3e-29, Method: Compositional matrix adjust. Identities = 104/343 (30%), Positives = 165/343 (48%), Gaps = 40/343 (11%) Query: 26 SHDYMKQVAVAADTLGYDGVL----LPTGRSCE---DAWVAAASLIDATRRLKFLVAVRP 78 S +Y +++AV A+ G++ L G E ++ + +L+ AT +LK L A+ P Sbjct: 42 SLEYNQRLAVTAEKAGFEYALSQIRFTAGYGAEYQHESVSFSQALLHATTKLKVLAAILP 101 Query: 79 GLMAPTLAARMAATFDRLSGGRLLINVVTGGDPVEQESDGV-FGSHAERYEISDEFLKIW 137 G P + A+ AT D +S GR+ INVV+G E ++ G + H ERY S+EF++ Sbjct: 102 GPWHPAVVAKQIATIDHISNGRIAINVVSGWFKGEFQAIGEPWLEHDERYRRSNEFIRAL 161 Query: 138 RDVLTK---TRSGEPTHFQGKHLSVKGAKALYPPVQQPYPPLYFGGSSDEAIDLAAEQVD 194 + + T+ T G+ F LS K PVQ+P+P ++ GGSS A D AA D Sbjct: 162 KGIWTQDDFTFKGDFYRFNNYTLSPK-------PVQKPHPEIFQGGSSRAARDNAASVSD 214 Query: 195 VYLTWGEPPAAVAEKIARVRERAAKAGRTLRFGIRLHVIVRETNEAAWRAADELISRLDD 254 Y T G + +I ++ +AA+ G +R G+ VI R+T E A ++I Sbjct: 215 WYFTNGNTLEGLKAQIDDIQAKAAQNGHKVRIGVNAFVIARDTEEEAQAVLQDIIDHAHV 274 Query: 255 ETI-----AKAQANFARMDSEGQRRMAALHGGRRDKLEVSPNLWAGVGLVRGGAGTALVG 309 E + A QA A + EG + D ++ + G T L+G Sbjct: 275 EAVHAFGDAVKQAGKASPEGEGNWAKSTF----EDLVQYN-----------DGFKTNLIG 319 Query: 310 DGPTVAERLQEYAALGIDTFILSGYPH-LEESYRFAELVFPLL 351 +AER+ A+G+D +L+G+ H +EE F + V PL+ Sbjct: 320 TPQQIAERIVALKAIGVD-LVLTGFLHFIEEVEYFGQKVLPLV 361 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.136 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,319,291 Number of extensions: 59138 Number of successful extensions: 205 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 202 Number of HSP's successfully gapped: 4 Length of query: 388 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 301 Effective length of database: 1,207,925 Effective search space: 363585425 Effective search space used: 363585425 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)