BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0029.0174.p (327 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310977|ref|YP_003775069.1| alkanesulfonates ABC transporte... 651 0.0 gi|300309984|ref|YP_003774076.1| nitrate/sulfonate/bicarbonate A... 290 1e-79 gi|300310915|ref|YP_003775007.1| alkanesulfonates ABC transporte... 281 6e-77 gi|300313870|ref|YP_003777962.1| nitrate/sulfonate/bicarbonate A... 253 2e-68 gi|300309986|ref|YP_003774078.1| nitrate/sulfonate/bicarbonate A... 137 1e-33 >gi|300310977|ref|YP_003775069.1| alkanesulfonates ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 327 Score = 651 bits (1680), Expect = 0.0, Method: Compositional matrix adjust. Identities = 326/327 (99%), Positives = 326/327 (99%) Query: 1 MSANKNKTVHQARRRAIQCLSAIALGALTIALGASPVQAQETKLLRIGYQKAANTLVLLK 60 MSANKNKTVHQARRRAIQ LSAIALGALTIALGASPVQAQETKLLRIGYQKAANTLVLLK Sbjct: 1 MSANKNKTVHQARRRAIQRLSAIALGALTIALGASPVQAQETKLLRIGYQKAANTLVLLK 60 Query: 61 AHGTLEKRLQPLGVEVKWAEFAAGPQLLEALNVGSVDFGYVGEAPPVFAQAAGADFVYTA 120 AHGTLEKRLQPLGVEVKWAEFAAGPQLLEALNVGSVDFGYVGEAPPVFAQAAGADFVYTA Sbjct: 61 AHGTLEKRLQPLGVEVKWAEFAAGPQLLEALNVGSVDFGYVGEAPPVFAQAAGADFVYTA 120 Query: 121 YEIPTPDAEGLLVQKNSPIKSVAELKGKKIAFNKGSDVHWLVVALLKKAGLQYSDIQPVY 180 YEIPTPDAEGLLVQKNSPIKSVAELKGKKIAFNKGSDVHWLVVALLKKAGLQYSDIQPVY Sbjct: 121 YEIPTPDAEGLLVQKNSPIKSVAELKGKKIAFNKGSDVHWLVVALLKKAGLQYSDIQPVY 180 Query: 181 LAPADARAAFERGAVDAWAIWDPFQAAAEKQVGARRLATGVGAVNHHQFFLSARPFAAKN 240 LAPADARAAFERGAVDAWAIWDPFQAAAEKQVGARRLATGVGAVNHHQFFLSARPFAAKN Sbjct: 181 LAPADARAAFERGAVDAWAIWDPFQAAAEKQVGARRLATGVGAVNHHQFFLSARPFAAKN 240 Query: 241 PQVEKILLEEISREGEWVRTHTAQAAAQLAPIQGLDADIIETGLKRYAHVYKPVDAQVLA 300 PQVEKILLEEISREGEWVRTHTAQAAAQLAPIQGLDADIIETGLKRYAHVYKPVDAQVLA Sbjct: 241 PQVEKILLEEISREGEWVRTHTAQAAAQLAPIQGLDADIIETGLKRYAHVYKPVDAQVLA 300 Query: 301 EQQKIADAFYELKLIPKPLKVSDAVLK 327 EQQKIADAFYELKLIPKPLKVSDAVLK Sbjct: 301 EQQKIADAFYELKLIPKPLKVSDAVLK 327 >gi|300309984|ref|YP_003774076.1| nitrate/sulfonate/bicarbonate ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 328 Score = 290 bits (741), Expect = 1e-79, Method: Compositional matrix adjust. Identities = 144/320 (45%), Positives = 210/320 (65%), Gaps = 4/320 (1%) Query: 8 TVHQARRRAIQCLSAIALGALTIALGASPVQAQETKLLRIGYQKAANTLVLLKAHGTLEK 67 + RRR + +A+ LG+ AL + V AQ K +RIGYQK++ L +LKA+GTLEK Sbjct: 5 STDSTRRRLLHAATALGLGS---ALAPASVFAQGNKAIRIGYQKSSTLLTILKANGTLEK 61 Query: 68 RLQPLGVEVKWAEFAAGPQLLEALNVGSVDFGY-VGEAPPVFAQAAGADFVYTAYEIPTP 126 L P G ++ W EFA+G LLEALNVG VD V + PVFAQAAGA Y A E P+P Sbjct: 62 LLAPTGAKISWHEFASGLPLLEALNVGGVDLSADVADTVPVFAQAAGAKLAYVAQESPSP 121 Query: 127 DAEGLLVQKNSPIKSVAELKGKKIAFNKGSDVHWLVVALLKKAGLQYSDIQPVYLAPADA 186 A+ ++V+ +S ++ ++LKGKKIA K + VH+L++A L+KAGL++SD+ YL+PAD Sbjct: 122 AAQAIVVRADSVLRKASDLKGKKIAVTKAAGVHYLLIATLEKAGLKFSDVDAAYLSPADG 181 Query: 187 RAAFERGAVDAWAIWDPFQAAAEKQVGARRLATGVGAVNHHQFFLSARPFAAKNPQVEKI 246 RAAFERG+VDAW WDPF A ++Q R L+ G G ++ +++L++ FA P+V ++ Sbjct: 182 RAAFERGSVDAWVTWDPFLAGVQRQSQVRILSDGRGVADYQRYYLASASFAQARPEVLRV 241 Query: 247 LLEEISREGEWVRTHTAQAAAQLAPIQGLDADIIETGLKRYAHVYKPVDAQVLAEQQKIA 306 + +E+ + G WV+ H +AAA LAP+ GLDAD IE R ++ + V + L EQQ+IA Sbjct: 242 VFDELQKTGHWVKQHPKEAAAFLAPLWGLDADTIELANSRRSYEVRAVRPEALGEQQRIA 301 Query: 307 DAFYELKLIPKPLKVSDAVL 326 D F+ L+P+ + +D + Sbjct: 302 DTFFAAGLLPRKINTNDVAI 321 >gi|300310915|ref|YP_003775007.1| alkanesulfonates ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 336 Score = 281 bits (718), Expect = 6e-77, Method: Compositional matrix adjust. Identities = 151/327 (46%), Positives = 211/327 (64%), Gaps = 7/327 (2%) Query: 3 ANKNKTVHQA-----RRRAIQCLSAIALGALTIALGASPVQAQETKLLRIGYQKAANTLV 57 A +NK + A RRR I + A A + V A + LR+GYQK A L Sbjct: 6 AQRNKAIQTAVRSPRRRRLIAGAVTLGAAAAAGASLPAWVHAAAPRQLRVGYQKGA--LS 63 Query: 58 LLKAHGTLEKRLQPLGVEVKWAEFAAGPQLLEALNVGSVDFGYVGEAPPVFAQAAGADFV 117 L++AHG LE+RL PLG VKW EF+AGP LEAL GS+DFG VGEAPP+FAQAAG V Sbjct: 64 LIRAHGVLEQRLAPLGATVKWVEFSAGPVQLEALGAGSIDFGDVGEAPPIFAQAAGTPLV 123 Query: 118 YTAYEIPTPDAEGLLVQKNSPIKSVAELKGKKIAFNKGSDVHWLVVALLKKAGLQYSDIQ 177 Y +P P E +LV+ +S ++ +LKGK+IA NKGS+VH+ +V LL+K GL+Y D+ Sbjct: 124 YVGATVPRPYVEAVLVKDDSELQRPQQLKGKRIALNKGSNVHYFLVKLLEKHGLKYGDVT 183 Query: 178 PVYLAPADARAAFERGAVDAWAIWDPFQAAAEKQVGARRLATGVGAVNHHQFFLSARPFA 237 PV+LAPADARAAFERGAVDAWAIW+P+ AAA++ + AR LA G V + ++ S R +A Sbjct: 184 PVWLAPADARAAFERGAVDAWAIWEPYLAAAQQGLKARILADADGVVKNRAYYFSTRDYA 243 Query: 238 AKNPQVEKILLEEISREGEWVRTHTAQAAAQLAPIQGLDADIIETGLKRYAHVYKPVDAQ 297 + + +I LEE+ + W + + A++LA I GL +++ ++RY + P+ Sbjct: 244 RDHADLLRIALEELGKIDAWAQARPDEYASELAGILGLPRSVLDVVVRRYHYGTLPITRA 303 Query: 298 VLAEQQKIADAFYELKLIPKPLKVSDA 324 +L EQQ+IAD F +L+LIP+ + + +A Sbjct: 304 ILDEQQQIADTFLDLRLIPRKISLLEA 330 >gi|300313870|ref|YP_003777962.1| nitrate/sulfonate/bicarbonate ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 323 Score = 253 bits (645), Expect = 2e-68, Method: Compositional matrix adjust. Identities = 139/317 (43%), Positives = 191/317 (60%), Gaps = 4/317 (1%) Query: 10 HQARRRAIQCLSAIALGALTIALGASPVQAQETKLLRIGYQKAANTLVLLKAHGTLEKRL 69 HQ R + L +A GAL A G V+A K+LRIGYQK NTL +LK G LE+ L Sbjct: 4 HQPRHKRRLLLQGLAAGAL--ASGTPLVRAAADKVLRIGYQKF-NTLNILKGSGKLEQAL 60 Query: 70 QPLGVEVKWAEFAAGPQLLEALNVGSVDFGYVGEAPPVFAQAAGADFVYTAYEIPTPDAE 129 QP G VKW EFAAGPQL EALN ++D G+ + P VFA A+G + VY E P P Sbjct: 61 QPQGWSVKWFEFAAGPQLTEALNANAIDLGHAADTPSVFANASGVNAVYLGAEQPYPKGI 120 Query: 130 GLLVQKNSPIKSVAELKGKKIAFNKGSDVHWLVVALLKKAGLQYSDIQPVYLA-PADARA 188 G+ V + SPI+SVA+LKGKK+A +G +V +L++ L++ GL Y DI+PVY+ AD RA Sbjct: 121 GIFVAQESPIRSVADLKGKKVATGRGWNVQFLLLKALEEVGLSYRDIEPVYVTNAADVRA 180 Query: 189 AFERGAVDAWAIWDPFQAAAEKQVGARRLATGVGAVNHHQFFLSARPFAAKNPQVEKILL 248 AF+ G VDA +WDPF A E R L G G N+ F L+ F ++ + + + Sbjct: 181 AFQSGNVDAATLWDPFLAGQELATRPRILRDGTGLSNNRTFHLAVPSFVDRHKDIVRTVF 240 Query: 249 EEISREGEWVRTHTAQAAAQLAPIQGLDADIIETGLKRYAHVYKPVDAQVLAEQQKIADA 308 E+ + +W ++H + A LAP G+D ++ +R + VDA ++AEQQ+IADA Sbjct: 241 AELKKTNQWTQSHPQETAELLAPQLGVDPKVLRLATERRNYTTVAVDAGIVAEQQQIADA 300 Query: 309 FYELKLIPKPLKVSDAV 325 F +L LI P++V D V Sbjct: 301 FLKLGLIKNPVRVQDKV 317 >gi|300309986|ref|YP_003774078.1| nitrate/sulfonate/bicarbonate ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 328 Score = 137 bits (344), Expect = 1e-33, Method: Compositional matrix adjust. Identities = 101/276 (36%), Positives = 143/276 (51%), Gaps = 7/276 (2%) Query: 14 RRAIQCLSAIALGALTIALGASPV---QAQETKLLRIGYQKAANTLVLLKAHGTLEKRLQ 70 RR L+ ALG ALG P+ AQ K++R+ Y + ++L+ G LE+ L+ Sbjct: 3 RRDFLRLAPAALG--VTALGGIPLLSSAAQPPKVIRLDYAYYSPPSLVLRKFGWLEEDLK 60 Query: 71 PLGVEVKWAEFAAGPQLLEALNVGSVDFGYVGEAPPVFAQAAGADFVYTAYEIPTPDAEG 130 G EVKW + LE LN SVDFG + A+A G + V Y P+ Sbjct: 61 AQGTEVKWVLSQGSNRALEYLNSDSVDFGSTAGLAALLARANG-NPVKAVYIYSRPEWTA 119 Query: 131 LLVQKNSPIKSVAELKGKKIAFNKGSDVHWLVVALLKKAGLQYSDIQPVYLAPADARAAF 190 L V K SP+KS+ ELKGKKIA KG+D + ++ L + GL+ SD++ V+L AD RAA Sbjct: 120 LAVGKESPVKSINELKGKKIAATKGTDPYLFLLRALHENGLKKSDVEIVHLQHADGRAAL 179 Query: 191 ERGAVDAWAIWDPFQAAAEKQVGARRLATGVGAVNHHQFFLSARPFAAKNPQVEKILLEE 250 E+G V AWA DP AA+E + G+R + V N + F + FAA++P K ++ Sbjct: 180 EQGRVAAWAGLDPHLAASELEAGSRLIYRNVN-FNTYGFLNVSDSFAARHPDQVKRVIAA 238 Query: 251 ISREGEWVRTHTAQAAAQLAPIQGLDADIIETGLKR 286 R W+ H + A L+ L A + LKR Sbjct: 239 YERARSWIIAHPDETVALLSEESKLSAPVARLQLKR 274 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.132 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 948,928 Number of extensions: 39008 Number of successful extensions: 176 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 169 Number of HSP's successfully gapped: 7 Length of query: 327 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 242 Effective length of database: 1,217,395 Effective search space: 294609590 Effective search space used: 294609590 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)