BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0029.0156.p (326 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310960|ref|YP_003775052.1| dehydrogenase oxidoreductase [H... 663 0.0 gi|300310832|ref|YP_003774924.1| D-isomer specific 2-hydroxyacid... 239 3e-64 gi|300313156|ref|YP_003777248.1| D-2-hydroxyacid dehydrogenase [... 213 2e-56 gi|300313735|ref|YP_003777827.1| D-3-phosphoglycerate dehydrogen... 143 2e-35 gi|300311988|ref|YP_003776080.1| D-3-phosphoglycerate dehydrogen... 142 5e-35 >gi|300310960|ref|YP_003775052.1| dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 326 Score = 663 bits (1711), Expect = 0.0, Method: Compositional matrix adjust. Identities = 324/326 (99%), Positives = 324/326 (99%) Query: 1 MKPKIFVARAVFPEVLARLEQHFEVESNQDDRIYSAAELQAKAQGKDGLFTTPSEPVAAA 60 MKPKIFVARAVFPEVLARLEQHFEVESNQDDRIYSAAELQAKAQGKDGLFTTPSEPV AA Sbjct: 1 MKPKIFVARAVFPEVLARLEQHFEVESNQDDRIYSAAELQAKAQGKDGLFTTPSEPVTAA 60 Query: 61 LFAANPQLKAVCNMAVGYNNIDVAAATQAGVMATNTPDVLNETTADFGWALMMAAAPRIT 120 LFAANPQLKAVCNMAVGYNNIDVAAATQAGVMATNTPDVLNETTADFGWALMMAAA RIT Sbjct: 61 LFAANPQLKAVCNMAVGYNNIDVAAATQAGVMATNTPDVLNETTADFGWALMMAAARRIT 120 Query: 121 ESEHFLRAGKWKKWSYDSFVGPDIHGATLGIIGMGRIGQAIARRSLGFDMQVLYHNRSRL 180 ESEHFLRAGKWKKWSYDSFVGPDIHGATLGIIGMGRIGQAIARRSLGFDMQVLYHNRSRL Sbjct: 121 ESEHFLRAGKWKKWSYDSFVGPDIHGATLGIIGMGRIGQAIARRSLGFDMQVLYHNRSRL 180 Query: 181 APELEARANHARYVSKEELLRQADHVILVLPYSKDTHHTIAAAELALMKPTATLTNIARG 240 APELEARANHARYVSKEELLRQADHVILVLPYSKDTHHTIAAAELALMKPTATLTNIARG Sbjct: 181 APELEARANHARYVSKEELLRQADHVILVLPYSKDTHHTIAAAELALMKPTATLTNIARG 240 Query: 241 GIVDDAALIAALREGRIAAAGVDVFENEPAFKPEFLDLSNVVLTPHIASASTPTRLAMAN 300 GIVDDAALIAALREGRIAAAGVDVFENEPAFKPEFLDLSNVVLTPHIASASTPTRLAMAN Sbjct: 241 GIVDDAALIAALREGRIAAAGVDVFENEPAFKPEFLDLSNVVLTPHIASASTPTRLAMAN 300 Query: 301 CAADNLIAALSGQRPPNLLNPDVVKG 326 CAADNLIAALSGQRPPNLLNPDVVKG Sbjct: 301 CAADNLIAALSGQRPPNLLNPDVVKG 326 >gi|300310832|ref|YP_003774924.1| D-isomer specific 2-hydroxyacid dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 319 Score = 239 bits (610), Expect = 3e-64, Method: Compositional matrix adjust. Identities = 147/319 (46%), Positives = 194/319 (60%), Gaps = 13/319 (4%) Query: 1 MKPKIFVARAVFPEVLARLEQHFEV----ESNQDDRIYSAAELQAKAQGKDGLFTTPSEP 56 MKP + V + + ++ RL+ F++ D+R A L A A G G S P Sbjct: 1 MKPHVVVYKKLPEHLVQRLQAEFDLTLFDRITDDNRADFIAAL-ATAHGMIG----ASLP 55 Query: 57 VAAALFAANPQLKAVCNMAVGYNNIDVAAATQAGVMATNTPDVLNETTADFGWALMMAAA 116 + A+ A PQLK V ++VG +N D+ Q G+M +TP VL E TAD +AL++A+A Sbjct: 56 ITNAMLEAAPQLKIVSTISVGVDNFDLDYFRQRGLMLAHTPGVLTEATADTIFALILASA 115 Query: 117 PRITESEHFLRAGKWKKWSYDSFVGPDIHGATLGIIGMGRIGQAIARRS-LGFDMQVLYH 175 R+ E +++AG+WK ++ G ++HG TLG+IGMGRIG A+ARR+ GF M +LYH Sbjct: 116 RRVVELAEYVKAGRWKGSIGEAQFGVNVHGKTLGLIGMGRIGSAVARRAHHGFGMPILYH 175 Query: 176 NRSRLAPELEARANHARYVSKEELLRQADHVILVLPYSKDTHHTIAAAELALMKPTATLT 235 NR R PE E R ARYVSKE+LL QAD V ++LP + T A E ALMK +A Sbjct: 176 NR-RPDPEAE-RELGARYVSKEDLLAQADFVCVMLPLTPQTERMFGAPEFALMKRSAIFI 233 Query: 236 NIARGGIVDDAALIAALREGRIAAAGVDVFENEPA-FKPEFLDLSNVVLTPHIASASTPT 294 N +RG IVD+AALIAAL++ I AG+DVFE EP + L L NVV PHI SA+ T Sbjct: 234 NASRGRIVDEAALIAALQDKTIHGAGLDVFEVEPLPLQSPLLKLPNVVALPHIGSATHET 293 Query: 295 RLAMANCAADNLIAALSGQ 313 RLAMA A NLIA L G+ Sbjct: 294 RLAMAELAVTNLIAGLRGE 312 >gi|300313156|ref|YP_003777248.1| D-2-hydroxyacid dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 316 Score = 213 bits (542), Expect = 2e-56, Method: Compositional matrix adjust. Identities = 128/312 (41%), Positives = 177/312 (56%), Gaps = 11/312 (3%) Query: 2 KPKIFVARAVFPEVLARLEQHFEVESNQDDRIYSAAELQAKAQGKDGLFTTPSEPVAAAL 61 +P + + + +L L+QH+ N + +S AEL A A G+ V+ Sbjct: 6 RPHVLIVARLPQHLLDLLQQHYTCH-NLILQPHSEAELAAIAPQIRGIAANGEAKVSREF 64 Query: 62 FAANPQLKAVCNMAVGYNNIDVAAATQAGVMATNTPDVLNETTADFGWALMMAAAPRITE 121 A P L+ V VGY+ +DV AA + G+ T+TPDVLN+ AD ALM+A A + Sbjct: 65 MARFPALEIVSVFGVGYDGVDVPAARERGIHVTHTPDVLNDDVADLAMALMLATARNVVR 124 Query: 122 SEHFLRAGKWKKWSYDSFVGPDIHGATLGIIGMGRIGQAIARRSLGFDMQVLYHNRSRLA 181 ++ F R+G+WKK + + GA LGI+G+GRIGQAIA+R+ FDMQ+ YHNRSR Sbjct: 125 ADRFARSGEWKKGPFP--FTTKVSGARLGIVGLGRIGQAIAKRAAAFDMQISYHNRSR-- 180 Query: 182 PELEARANHARYV-SKEELLRQADHVILVLPYSKDTHHTIAAAELALMKPTATLTNIARG 240 + YV S L R+ D ++++ P T + A L + P L N+ARG Sbjct: 181 -----KDVPYTYVDSITALAREVDFLVMITPGGAGTRALVNAEVLEALGPKGFLINVARG 235 Query: 241 GIVDDAALIAALREGRIAAAGVDVFENEPAFKPEFLDLSNVVLTPHIASASTPTRLAMAN 300 +VD+AALIAAL+ G IA AG+DVF +EP E L NVVLTPH+AS + TR AMA+ Sbjct: 236 SVVDEAALIAALKTGVIAGAGLDVFADEPNVPAELAALDNVVLTPHMASGTLVTRTAMAD 295 Query: 301 CAADNLIAALSG 312 A +NL A SG Sbjct: 296 LAFNNLQAHFSG 307 >gi|300313735|ref|YP_003777827.1| D-3-phosphoglycerate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 308 Score = 143 bits (360), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 90/256 (35%), Positives = 132/256 (51%), Gaps = 9/256 (3%) Query: 57 VAAALFAANPQLKAVCNMAVGYNNIDVAAATQAGVMATNTPDVLNETTADFGWALMMAAA 116 V+A + A P L+ + G ++ID AA Q G+ A+ WAL+MA A Sbjct: 60 VSARVMDAAPILRVISKHGTGIDSIDSQAAQQRGIAVKAAAGANAPAVAEHTWALIMACA 119 Query: 117 PRITESEHFLRAGKWKKWSYDSFVGPDIHGATLGIIGMGRIGQAIARRSLGFDMQVLYHN 176 + + +R G W K ++ S ++ G TLG++G+G IG+ +A + M VL H+ Sbjct: 120 KNVVGLDQRMREGHWDKSTHKSL---ELQGRTLGLVGLGAIGRRVAAVAAALGMPVLAHD 176 Query: 177 RSRLAPELEARANHARYVSKEELLRQADHVILVLPYSKDTHHTIAAAELALMKPTATLTN 236 P + + V + L Q+D V L P + + H I A LA MK A L N Sbjct: 177 -----PYAKEAPQGVQLVDLDTLFAQSDVVSLHCPLTAENKHMINAQSLARMKDGAILVN 231 Query: 237 IARGGIVDDAALIAALREGRIAAAGVDVFENEPAFKPEFLD-LSNVVLTPHIASASTPTR 295 ARGG++D+ ALIAAL G++ AAG+D FE EP P L + N VL+PHI ++ Sbjct: 232 TARGGLIDEQALIAALDSGKLRAAGLDSFEKEPFTAPHPLQRVGNAVLSPHIGGVTSDAY 291 Query: 296 LAMANCAADNLIAALS 311 +AM AA N++A L Sbjct: 292 IAMGTGAASNVLAVLG 307 >gi|300311988|ref|YP_003776080.1| D-3-phosphoglycerate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 310 Score = 142 bits (357), Expect = 5e-35, Method: Compositional matrix adjust. Identities = 96/291 (32%), Positives = 138/291 (47%), Gaps = 9/291 (3%) Query: 1 MKPKIFVARAVFPEVLARLEQHFEVESNQDDRIYSAAELQAKAQGKDGLFTTPSEPVAAA 60 MKP + V + E A + H+++ DR + A+ + T S + A Sbjct: 1 MKPPLLVLIHLSEESTATIAAHYDI-LYAPDRQRRDEMIAGPARKVAVVLTNGSTGLTAE 59 Query: 61 LFAANPQLKAVCNMAVGYNNIDVAAATQAGVMATNTPDVLNETTADFGWALMMAAAPRIT 120 A P L+ VC + G+ N+DVA A G+ + AD L++A I Sbjct: 60 EMRALPHLQLVCTLGAGFENVDVAHAEAHGIEIATGAGTNEDCVADHALGLLLAILRNIP 119 Query: 121 ESEHFLRAGKWKKWSYDSFVGPDIHGATLGIIGMGRIGQAIARRSLGFDMQVLYHNRSRL 180 + + R G W++ + P + G +GI+GMG IG+ IARR+ FD ++ Y NR + Sbjct: 120 VLDRYTRDGGWRE---TIPLQPQLAGKRVGIVGMGNIGKKIARRAAAFDAEIAYCNRKK- 175 Query: 181 APELEARANHARYVSKEELLRQADHVILVLPYSKDTHHTIAAAELALMKPTATLTNIARG 240 ++ + +L AD +I+ P T H I A L + P L NI RG Sbjct: 176 ----RDDVDYHYFPDVAQLAGWADCLIVAAPGGAQTRHLINARVLEELGPQGYLVNIGRG 231 Query: 241 GIVDDAALIAALREGRIAAAGVDVFENEPAFKPEFLDLSNVVLTPHIASAS 291 IVD AL AAL GR+A AG+DV+E EP + L NVVLTPHIA S Sbjct: 232 SIVDTDALGAALSSGRLAGAGLDVYEGEPQPPAALIALPNVVLTPHIAGWS 282 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.132 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,038,601 Number of extensions: 44064 Number of successful extensions: 191 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 170 Number of HSP's successfully gapped: 11 Length of query: 326 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 241 Effective length of database: 1,217,395 Effective search space: 293392195 Effective search space used: 293392195 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)