BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0029.0153.p (420 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310957|ref|YP_003775049.1| branched-chain amino acid ABC t... 797 0.0 gi|300311772|ref|YP_003775864.1| branched-chain amino acid ABC t... 340 2e-94 gi|300312955|ref|YP_003777047.1| amino acid ABC transporter peri... 89 9e-19 gi|300312236|ref|YP_003776328.1| amino acid ABC transporter peri... 78 1e-15 gi|300311113|ref|YP_003775205.1| branched-chain amino acid ABC t... 73 4e-14 >gi|300310957|ref|YP_003775049.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 413 Score = 797 bits (2058), Expect = 0.0, Method: Compositional matrix adjust. Identities = 408/413 (98%), Positives = 409/413 (99%), Gaps = 2/413 (0%) Query: 8 HRKQSLHSPRSRWTLLLGLAVALALALPWGPSHAAEAIRIGEINSRHRLSTAGQAYRNGL 67 HRKQSLHSPRSRWTLLL +A ALALPWGPSHAAEAIRIGEINSRHRLSTAGQAYRNGL Sbjct: 3 HRKQSLHSPRSRWTLLL--GLAAALALPWGPSHAAEAIRIGEINSRHRLSTAGQAYRNGL 60 Query: 68 ALALEQINAAGGVGGRPLQLVVREDDGDADQAARNARALISQDHVEVLTGSMLSDVALAV 127 ALALEQINAAGGVGGRPLQLVVREDDGDADQAARNARALISQDHVEVLTGSMLSDVALAV Sbjct: 61 ALALEQINAAGGVGGRPLQLVVREDDGDADQAARNARALISQDHVEVLTGSMLSDVALAV 120 Query: 128 SREAARGRTVLVVGDALSDAITLDKGNRYTFRVRPSTYMQAAMLAEQAARLPGRRWAVVA 187 SREAARGRTVLVVGDALSDAITLDKGNRYTFRVRPSTYMQAAMLAEQAARLPGRRWAVVA Sbjct: 121 SREAARGRTVLVVGDALSDAITLDKGNRYTFRVRPSTYMQAAMLAEQAARLPGRRWAVVA 180 Query: 188 PNYEYGQSAVASFKLLLKQARPDVEFVSDQWPALGKIKADDVARAIRQSRPDGIFNATFG 247 PNYEYGQSAVASFKLLLKQARPDVEFVSDQWPALGKIKADDVARAIRQSRPDGIFNATFG Sbjct: 181 PNYEYGQSAVASFKLLLKQARPDVEFVSDQWPALGKIKADDVARAIRQSRPDGIFNATFG 240 Query: 248 EDLQKFVQAGNRQQLFEGVRVVSMASAGAPSLDPSQSEAIRDWLAIGYPWDQVSLPEHQR 307 EDLQKFVQAGNRQQLFEGVRVVSMASAGAPSLDPSQSEAIRDWLAIGYPWDQVSLPEHQR Sbjct: 241 EDLQKFVQAGNRQQLFEGVRVVSMASAGAPSLDPSQSEAIRDWLAIGYPWDQVSLPEHQR 300 Query: 308 FLEAYLKRFSERPQLSAVFGYTTMMAIAAGLKKSEGGDGEVLVEAMRGLQFGSPLGPVSF 367 FLEAYLKRFSERPQLSAVFGYTTMMAIAAGLKKSEGGDGEVLVEAMRGLQFGSPLGPVSF Sbjct: 301 FLEAYLKRFSERPQLSAVFGYTTMMAIAAGLKKSEGGDGEVLVEAMRGLQFGSPLGPVSF 360 Query: 368 RALDQQASLGAFVGLVSQKDGRSSMRDWRYLDGAAYLPPPAYVRSRRPASASK 420 RALDQQASLGAFVGLVSQKDGRSSMRDWRYLDGAAYLPPPAYVRSRRPASASK Sbjct: 361 RALDQQASLGAFVGLVSQKDGRSSMRDWRYLDGAAYLPPPAYVRSRRPASASK 413 >gi|300311772|ref|YP_003775864.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 404 Score = 340 bits (871), Expect = 2e-94, Method: Compositional matrix adjust. Identities = 168/376 (44%), Positives = 247/376 (65%) Query: 39 SHAAEAIRIGEINSRHRLSTAGQAYRNGLALALEQINAAGGVGGRPLQLVVREDDGDADQ 98 + A + IRIGEINS + YR G +AL++INA+GGV G+ L+++ R+D+G+ Sbjct: 26 ARAQDTIRIGEINSYKAQPAFLEPYRRGWEMALDEINASGGVLGKKLEVIARDDNGNPGD 85 Query: 99 AARNARALISQDHVEVLTGSMLSDVALAVSREAARGRTVLVVGDALSDAITLDKGNRYTF 158 + R A L++++ V +L G LS+ LA++ A + R + + L+D I GN+YT+ Sbjct: 86 SVRVAEELMARERVALLFGGFLSNTGLALTDYAKQHRVFFLAAEPLTDKIVWQNGNKYTY 145 Query: 159 RVRPSTYMQAAMLAEQAARLPGRRWAVVAPNYEYGQSAVASFKLLLKQARPDVEFVSDQW 218 R+RPST M A + ++A +L +RWA+V PNYEYGQSAVASFK L+KQA+PDVEFV++Q Sbjct: 146 RLRPSTSMLVASVVKEAVKLKKKRWAIVYPNYEYGQSAVASFKTLMKQAQPDVEFVAEQA 205 Query: 219 PALGKIKADDVARAIRQSRPDGIFNATFGEDLQKFVQAGNRQQLFEGVRVVSMASAGAPS 278 P LGKI A V +A+ ++PD IFNA F DL KFV+ GN + LFE V VVS+ + Sbjct: 206 PPLGKIDAGAVTQALADAKPDAIFNALFAADLGKFVREGNTRGLFEQVDVVSLLTGEPEY 265 Query: 279 LDPSQSEAIRDWLAIGYPWDQVSLPEHQRFLEAYLKRFSERPQLSAVFGYTTMMAIAAGL 338 LDP + EA ++W+ GYPW + PEH++F+EAY K+F++ P+ ++ GY+ +M+IAAGL Sbjct: 266 LDPLKGEAPQNWIVTGYPWYAIHTPEHKKFVEAYRKKFNDYPRNGSLVGYSALMSIAAGL 325 Query: 339 KKSEGGDGEVLVEAMRGLQFGSPLGPVSFRALDQQASLGAFVGLVSQKDGRSSMRDWRYL 398 +K+ D + L A GL+ +P+ + +R D Q++LGAFVG K+GR M + Y+ Sbjct: 326 RKAGSTDADRLAAAFSGLKVETPVASIVYRPQDHQSTLGAFVGRTGLKEGRGVMTSFTYI 385 Query: 399 DGAAYLPPPAYVRSRR 414 DGAA P A V+ R Sbjct: 386 DGAALQLPDAEVKKLR 401 >gi|300312955|ref|YP_003777047.1| amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 392 Score = 88.6 bits (218), Expect = 9e-19, Method: Compositional matrix adjust. Identities = 106/368 (28%), Positives = 166/368 (45%), Gaps = 50/368 (13%) Query: 22 LLLGLAVALALALPWGPSHAAEAIRIGEINSRHRLSTA-GQAYRNGLALALEQINAAGGV 80 +++ +AV +L P+ AA+ +++G + + ++A G R G LAL+ + + Sbjct: 11 VMMAMAVGASL-----PAQAADVVKVGFLTTLSGPASAPGVDEREGFMLALKTL--GNKL 63 Query: 81 GGRPLQLVVREDDGDADQAARNARALISQDHVEVLTGSMLSDVALAVSREAARGRTVLVV 140 GG P +++V +D D A + A LI ++ V++L+G + S++ LAV+ A +TV+V Sbjct: 64 GGVPAEVIVVDDQFSPDAAKQGADRLIKREKVDILSGFVYSNILLAVAPAAFASKTVVVS 123 Query: 141 GDALSDAITLDKGNRYTFRVRPSTYMQAAMLAEQAARLPG----RRWAVVAPNYEYGQSA 196 +A + N Y F S Q E A +L +R A++APNY G+ A Sbjct: 124 ANAGPAQLAGAGCNPYFF----SASYQNDAAHEAAGKLMADKGYQRVAIIAPNYPAGKDA 179 Query: 197 VASFKLLLKQARPDVEFVSDQWPALGKIKADDVARAIRQSRPDGIFNATF------GEDL 250 A FK LK+ P VE +P LG++ D A I Q R I F G + Sbjct: 180 SAGFKRFLKK-DPAVEL----FPKLGQL---DFATEIAQLRAANIDALYFFLPGGMGVNF 231 Query: 251 QK-FVQAGNRQQLFEGVRVVSMASAGAPSLDPSQSEAIRDWLAIGYP----WDQVSLPEH 305 K + AG ++ VRV+ P+ D Q L G W LP Sbjct: 232 VKQYAAAGMDPKI---VRVM-------PAYDADQQMLSNGDLIAGVANTGQWSS-DLPNE 280 Query: 306 QR--FLEAYLKRFSERPQLSAVFGYTTMMAIAAGLKKSEGG--DGEVLVEAMRGLQFGSP 361 Q F+ AYLK + +P + A Y M I + +K G + E L+ A++ F S Sbjct: 281 QNRAFVAAYLKEYGRQPSMFASQAYDAAMLIDSAVKAVGGKVENREALIAAIKTANFKSV 340 Query: 362 LGPVSFRA 369 G F A Sbjct: 341 RGGFRFAA 348 >gi|300312236|ref|YP_003776328.1| amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 385 Score = 78.2 bits (191), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 86/324 (26%), Positives = 142/324 (43%), Gaps = 27/324 (8%) Query: 60 GQAYRNGLALALEQINAAGGVGGRPLQLVVREDDGDADQAARNARALISQDHVEVLTGSM 119 G A +NG LA E+INA GGV G L LV+ ++ G ++A + LI QD V + G Sbjct: 44 GVAIKNGFTLASEEINAKGGVNGDKLALVIEDEQGKKEEAINVFKKLIFQDKVLAVFGPT 103 Query: 120 LSDVALAVSREAARGRTVLVVGDALSDAITLDKGNRYTFRVRPSTYMQAAML---AEQAA 176 LS+ A A A + V+ +D IT +TFR ++ M+A +L + A Sbjct: 104 LSNSAFAADPIANAAKVVVFGTSNTADGIT--AMGPFTFR---NSVMEADVLPVTVKAAV 158 Query: 177 RLPG-RRWAVVAPNYE-YGQSAVASFKLLLKQARPDVEFVSDQWPALGKIKADDVARAIR 234 + G ++ AV+ N + + +S FK L+ + + + + A G + I+ Sbjct: 159 KHFGIKKVAVIYGNDDAFTKSGYDVFKATLEHQK--IPVTTTEAYAKGDVDFKAQLTKIK 216 Query: 235 QSRPDGIFNATFGEDLQKFV---QAGNRQQLFEGVRVVSMASAGAPSLDPSQSEAIRDWL 291 PD I + E+ + +A +Q F G ++ +P L +A + L Sbjct: 217 AGNPDAIVCSCLAEEAANIILQTRALGMKQPFIGGNGLN-----SPKLFEIAKDAGDNTL 271 Query: 292 AIGYPWD-QVSLPEHQRFLEAYLKRFSERPQLSAVFGYTTMMAIAAGLKKSE-----GGD 345 +G PW + P ++ F+ AY +F P A Y M +A LK + D Sbjct: 272 -MGSPWSAENQTPANKAFITAYKAKFGADPDQFAAQAYDAMYIVADALKNVKLSGNLAKD 330 Query: 346 GEVLVEAMRGLQFGSPLGPVSFRA 369 + L A+ ++ G +FRA Sbjct: 331 RDALRAALPAVKIDGATGKFAFRA 354 >gi|300311113|ref|YP_003775205.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 383 Score = 72.8 bits (177), Expect = 4e-14, Method: Compositional matrix adjust. Identities = 99/383 (25%), Positives = 182/383 (47%), Gaps = 35/383 (9%) Query: 27 AVALALALPWGPSHAAEAIRIGEINSRHRLSTA--GQAYRNGLALALEQINAAGGVGGRP 84 A ++LAL S AA+ I+IG ++ + +A G + R+G+ LA+ +INAAGG+ GR Sbjct: 11 AALMSLALT---SQAADPIKIG-VSGPYTGGSAPMGVSMRDGVKLAVAEINAAGGLLGRQ 66 Query: 85 LQLVVREDDGDADQAARNARALISQDHVEVLTGSMLSDVALAVSREAARGRTVLVVGDAL 144 +QL+ R+D+ ++ + A+ LI+++ V G + + VALA R + ++ A Sbjct: 67 IQLIERDDEAKNERGVQIAQELINKEKVVATVGFINTGVALASQRFYQEAKIPVINNVAT 126 Query: 145 SDAITL---DKGNRYTFRVRPSTYMQAAMLAEQAA-RLPGRRWAVVAPNYEYGQSAVASF 200 IT + + Y FR + +QA M+A++A + + A++A + YGQ Sbjct: 127 GTVITKQFEKEKDNYVFRTSAADKIQAKMIADEAVDKQKFTKVAILADSTNYGQLGREDL 186 Query: 201 KLLL--KQARPDVEFVSDQWPALGKIKADDVARAIRQSRPDG---IFNATFGEDLQKFVQ 255 + +L K+ P +++ IK D+ + +S+ G + G +L + Sbjct: 187 EKVLADKKVTP---VAVEKY----NIKDVDMTAQLLKSKQGGAQAVLTYGIGPELAQI-- 237 Query: 256 AGNRQQLFEGVRVV-SMASAGAPSLDPSQSEAIRDWLAIGYPWDQVSLPEHQRFLEAYLK 314 A ++L V ++ S + +D S + + D + P+ + F+EAY K Sbjct: 238 ANGMEKLNWHVPIIGSWPLSMGNFIDNSGKNGNGARMPQTFIQDG-NTPKRKAFIEAYQK 296 Query: 315 --RFSERPQ-LSAVFGYTTMMAIAAGLKKSEGGDGEVLVEAMRGLQFGSPLGPVS----- 366 + P +SA GY +++ +AA +K++ DG+ + A+ L G V+ Sbjct: 297 AYKVDRMPSAVSAAQGYDSILLLAAAIKQAGTTDGDKVRAALENLNTKVE-GVVTTYDKP 355 Query: 367 FRALDQQASLGAFVGLVSQKDGR 389 F D +A A V + K+GR Sbjct: 356 FTHDDHEAIKPAMVVMGEVKNGR 378 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.132 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,322,910 Number of extensions: 55798 Number of successful extensions: 271 Number of sequences better than 1.0e-08: 15 Number of HSP's gapped: 268 Number of HSP's successfully gapped: 15 Length of query: 420 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 332 Effective length of database: 1,203,190 Effective search space: 399459080 Effective search space used: 399459080 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)