BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0029.0133.p (780 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310933|ref|YP_003775025.1| metal-transporting P-type ATPas... 1428 0.0 gi|300310937|ref|YP_003775029.1| heavy metal translocating P-typ... 333 2e-92 gi|300311699|ref|YP_003775791.1| cation transport P-type ATPase ... 229 6e-61 gi|300312503|ref|YP_003776595.1| cation transport P-type ATPase ... 196 9e-51 gi|300313072|ref|YP_003777164.1| high-affinity K+ transport syst... 113 6e-26 >gi|300310933|ref|YP_003775025.1| metal-transporting P-type ATPase transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 778 Score = 1428 bits (3697), Expect = 0.0, Method: Compositional matrix adjust. Identities = 739/750 (98%), Positives = 741/750 (98%), Gaps = 4/750 (0%) Query: 33 APATSSCCAGHACASEQERSEPIPAARLEGATEEAVFFIQKMDCPTEEKLIRERLSQMSG 92 APATSSCCAGHACASEQERS PIPAARLEGATEEAVFFIQKMDCPTEEKLIRERLSQMSG Sbjct: 31 APATSSCCAGHACASEQERSAPIPAARLEGATEEAVFFIQKMDCPTEEKLIRERLSQMSG 90 Query: 93 VGTLEFNLIQRELTVQHQLPSIAPLVATLKTLDMLPAVKSDSLDAEAGRDAPDQAAAYRI 152 VGTLEFNLIQRELTVQHQLPSIAPLVATLK LDMLPAVKSDSLDAEAGRDAPDQAAAYRI Sbjct: 91 VGTLEFNLIQRELTVQHQLPSIAPLVATLKALDMLPAVKSDSLDAEAGRDAPDQAAAYRI 150 Query: 153 PARRWILLGLAGVAALGAEVLAWTSGDERSLPVIGLALAAILLGGLGTLKKGWIALRNFS 212 PARRWILLGLAGVAALGAEVLAW+SGDERSLPVIGLALAAILLGGLGTLKKGWIALRNFS Sbjct: 151 PARRWILLGLAGVAALGAEVLAWSSGDERSLPVIGLALAAILLGGLGTLKKGWIALRNFS 210 Query: 213 LNMNFLMSLAVIGAAVIGQWPEAAVVIVLFTLAEMIEALSLDRARNAIAGLMAMTPDMAT 272 LNMNFLMSLAVIGAAVIGQWPEAAVVIVLFTLAEMIEALSLDRARNAIAGL+AMTPDMAT Sbjct: 211 LNMNFLMSLAVIGAAVIGQWPEAAVVIVLFTLAEMIEALSLDRARNAIAGLLAMTPDMAT 270 Query: 273 RRGEDGVWSTVPARTVTVDDTVRVAPGERVPLDGLVLSGSTSINQAPITGESIPVQKQAG 332 RRGEDGVWSTVPARTVTVDDTVRVAPGERVPLDGLVLSGSTSINQAPITGESIPVQKQAG Sbjct: 271 RRGEDGVWSTVPARTVTVDDTVRVAPGERVPLDGLVLSGSTSINQAPITGESIPVQKQAG 330 Query: 333 DPVFAGTINEQGAFEMRVTAVQGDSTLARIVKSVQQAQGQRAPTQRFVDNFARYYIPAVV 392 DPVFAGTINEQGAFEMRVTA QGDSTLARIVKSVQQAQGQRAPTQRFVDNFARYYIPAVV Sbjct: 331 DPVFAGTINEQGAFEMRVTAAQGDSTLARIVKSVQQAQGQRAPTQRFVDNFARYYIPAVV 390 Query: 393 VMAVLVALLPPLLAGAAFQPWLYKALVLLV--IACPCALVISTPVTIVSGLASAARHGIL 450 VMAVLVALLPPLLAGAAFQPWLYKALVLLV CPCALVISTPVTIVSGLASAARHGIL Sbjct: 391 VMAVLVALLPPLLAGAAFQPWLYKALVLLVIA--CPCALVISTPVTIVSGLASAARHGIL 448 Query: 451 VKGGVYLEQGRQLRALALDKTGTLTFGKPRVTDVVSLQAQADQQALLQLAVSLAARSDHP 510 VKGGVYLEQGRQLRALALDKTGTLTFGKPRVTDVVSLQAQADQQALLQLAVSLAARSDHP Sbjct: 449 VKGGVYLEQGRQLRALALDKTGTLTFGKPRVTDVVSLQAQADQQALLQLAVSLAARSDHP 508 Query: 511 VSRAIAAQEEIATVEVSEFAALAGRGVQGCINGQLLHLGNHRLVHELGQCSPELEARLQA 570 VSRAIAAQEEIATVEVSEFAALAGRGVQG INGQLLHLGNHRLVHELGQCSPELEARLQA Sbjct: 509 VSRAIAAQEEIATVEVSEFAALAGRGVQGRINGQLLHLGNHRLVHELGQCSPELEARLQA 568 Query: 571 FEKQGKTTTVLCLDGQPQLLVAVADTVRPSSRQAVAELRALGVEVIMLTGDNSHTAQAIA 630 FEKQGKTTTVLCLDGQPQLLVAVADTVRPSSRQAVAELRALGVEVIMLTGDNSHTAQAIA Sbjct: 569 FEKQGKTTTVLCLDGQPQLLVAVADTVRPSSRQAVAELRALGVEVIMLTGDNSHTAQAIA 628 Query: 631 AQTGISDARGDQLPEDKLKVIEELSQRHGVRGVGMVGDGINDAPALARADIGFAMGAAGT 690 AQTGISDARGDQLPEDKLK IEELSQRHGVRGVGMVGDGINDAPALARADIGFAMGAAGT Sbjct: 629 AQTGISDARGDQLPEDKLKAIEELSQRHGVRGVGMVGDGINDAPALARADIGFAMGAAGT 688 Query: 691 DTALETADVALMDDDLRKIPDFIRLSRKAHAVLVQNISIALGIKAVFLGLALVGMSSLWM 750 DTALETADVALMDDDLRKIPDFIRLSRKAHAVLVQNISIALGIKAVFLGLALVGMSSLWM Sbjct: 689 DTALETADVALMDDDLRKIPDFIRLSRKAHAVLVQNISIALGIKAVFLGLALVGMSSLWM 748 Query: 751 AVFADMGASLIVVFNGLRLVRGLRQRKAAA 780 AVFADMGASLIVVFNGLRLVRGLRQRKAAA Sbjct: 749 AVFADMGASLIVVFNGLRLVRGLRQRKAAA 778 >gi|300310937|ref|YP_003775029.1| heavy metal translocating P-type ATPase [Herbaspirillum seropedicae SmR1] Length = 744 Score = 333 bits (855), Expect = 2e-92, Method: Compositional matrix adjust. Identities = 227/596 (38%), Positives = 337/596 (56%), Gaps = 34/596 (5%) Query: 204 GWIALRNFSLNMNFLMSLAVIGAAVIGQW--------------------PEAAVVIVLFT 243 GW ALR S NM+ L++L A + W +AVVI L Sbjct: 144 GWGALRARSGNMDLLVALGTSAAYGLSLWQWLTLPTMPGHAMAMPHLYFESSAVVITLVL 203 Query: 244 LAEMIEALSLDRARNAIAGLMAMTPDMATRRGEDGVWSTVPARTVTVDDTVRVAPGERVP 303 L + +E + + +AIA L A+ P+ A R DG VP V V D V + PGERVP Sbjct: 204 LGKWLEGRAKRQTADAIAALNALRPENARVR-RDGQDIEVPLAQVRVGDLVVLRPGERVP 262 Query: 304 LDGLVLSGSTSINQAPITGESIPVQKQAGDPVFAGTINEQGAFEMRVTAVQGDSTLARIV 363 +DG+V G + +++A +TGES P KQAGDP+ AG+IN +G M TA+ ++ LARIV Sbjct: 263 VDGVVREGRSHLDEAMLTGESRPQAKQAGDPLTAGSINAEGVLVMETTAIGAETVLARIV 322 Query: 364 KSVQQAQGQRAPTQRFVDNFARYYIPAVVVMAVLVALLPPLLAGAAFQPWLYKALVLLVI 423 + V+QAQ +AP QR VD + ++PAV+++++L L L+ G Q L A+ ++VI Sbjct: 323 RMVEQAQAAKAPIQRLVDQVSAVFVPAVLLLSLLTLLGWGLVQGNWEQA-LLNAVAVMVI 381 Query: 424 ACPCALVISTPVTIVSGLASAARHGILVKGGVYLEQGRQLRALALDKTGTLTFGKPRVTD 483 ACPCAL ++TP I++G AARHGIL+K LE ++ A+A DKTGTLT G PR+ + Sbjct: 382 ACPCALGLATPTAIMAGTGVAARHGILIKDAEALEIAHRVNAVAFDKTGTLTLGHPRLLE 441 Query: 484 V-VSLQAQADQQALLQLAVSLAARSDHPVSRAIAAQEEIATVEVSEFA---ALAGRGVQG 539 + + Q D+ L A+ A S+HP+ RA+ + + ++AG G++G Sbjct: 442 LEATPSTQPDELLALAAALQ--AHSEHPLGRAVREAAAQSAQAARQAEQVRSVAGLGIEG 499 Query: 540 CINGQLLHLGNHRLVHELGQCSPELEARLQAFEKQGKTTTVLCLDGQPQL----LVAVAD 595 + G+ L LG+ R + +L +P L+AR + + QG+T + L + L+A D Sbjct: 500 VVEGRQLMLGSSRWMQQLQAHAPALQARAEQLQAQGRTLSWLARRNDSGVEVLGLLAFGD 559 Query: 596 TVRPSSRQAVAELRALGVEVIMLTGDNSHTAQAIAAQTGISDARGDQLPEDKLKVIEELS 655 ++P ++ A+A L +GV ML+GDN AQ +A++ GI + R + LP DK + + L Sbjct: 560 QIKPGAQAAIATLHRMGVRSAMLSGDNLGAAQQVASELGIQEVRAEVLPADKAEAVTALR 619 Query: 656 QRHGVRGVGMVGDGINDAPALARADIGFAMGAAGTDTALETADVALMDDDLRKIPDFIRL 715 Q G + V MVGDG+NDAPALA AD+G AM + GTD A++ A V LM D + D I + Sbjct: 620 Q-GGAQVVAMVGDGVNDAPALAAADVGMAM-STGTDVAMQAAGVTLMHGDPGLVADAIDI 677 Query: 716 SRKAHAVLVQNISIALGIKAVFLGLALVGMSSLWMAVFADMGASLIVVFNGLRLVR 771 SR+ +A + QN+ A V + LA +G+ + +A A +S+ VV N L L R Sbjct: 678 SRRTYAKIRQNLFWAFIYNLVGIPLAALGLLNPMLAGAAMAFSSVSVVSNALLLRR 733 >gi|300311699|ref|YP_003775791.1| cation transport P-type ATPase [Herbaspirillum seropedicae SmR1] Length = 734 Score = 229 bits (584), Expect = 6e-61, Method: Compositional matrix adjust. Identities = 195/591 (32%), Positives = 323/591 (54%), Gaps = 31/591 (5%) Query: 190 LAAILLGGLGTLKKGWIALRNFSLNMNFLMSLAVIGAAVIGQWPEAAVVIVLFTLAEMIE 249 LA L+ + L +A+R + ++ + +++ GA ++ + AAV+ ++F +E Sbjct: 12 LAGPLVVLMAVLADTAMAMRRGGMKLDLIALVSIAGAVLLREDLVAAVIALMFAGGRALE 71 Query: 250 ALSLDRARNAIAGLMAMTPDMATR-RGEDGVWSTVPARTVTVDDTVRVAPGERVPLDGLV 308 +++ RA+ ++ L+ P +A R GE V T+ V D + V PGE VP+DG + Sbjct: 72 SVAQRRAQQEMSALLGKAPHVANRYEGERIV--TIGLNLVRPGDRLLVRPGEPVPVDGRL 129 Query: 309 LSGSTSINQAPITGESIPVQKQAGDPVFAGTINEQGAFEMRVTAVQGDSTLARIVKSVQQ 368 L G ++++ +TGES+PV G + +G +N AF++ T+ ST A++++ VQ Sbjct: 130 L-GPAVVDESMLTGESLPVSHPQGATLASGCVNAGNAFDLLATSTDQSSTFAQVLRLVQA 188 Query: 369 AQGQRAPTQRFVDNFARYYIPAVVVMAVLVALLPPLLAGAAFQPWLYKALVLLVIACPCA 428 AQ +AP R D +A +IP V+ MA L LL P +AL ++V+A PC Sbjct: 189 AQASQAPAARMADRYALLFIPLVLAMAALSWLL-------TGDP--RRALAVVVVATPCP 239 Query: 429 LVISTPVTIVSGLASAARHGILVKGGVYLEQGRQLRALALDKTGTLTFGKPRVTDVVSLQ 488 L+++ PV IV ++ A+ G+L+K G LE+ Q+R L DKTGTLT GK R+ ++V+ Sbjct: 240 LILAVPVAIVCAMSRCAQRGVLIKHGGALEKLAQVRTLFFDKTGTLTAGKARLMNMVTTP 299 Query: 489 A-QADQQALLQLAVSLAARSDHPVSRAI--AAQEEIATVEV-SEFAALAGRGVQGCINGQ 544 + QAD+ LL+LA SL S+H +++A+ AA E + + + G G+ G ++G+ Sbjct: 300 SIQADE--LLRLAASLEQMSNHVIAQAVVSAAIERALVLSIPDQVQEQPGAGLSGRVDGK 357 Query: 545 LLHLGNHRLVHELGQCSPELEARLQAFEKQGKTTTVLCLDGQPQLLVAVADTVRPSSRQA 604 L+ +G + V P + +G + +DGQ ++ +AD +R + +A Sbjct: 358 LVRVGAMQYVIGPSAAPPWTSGLRDRLKYEGGAGVCVAVDGQLCGVLYLADDIRLDTPRA 417 Query: 605 VAELRALGVE-VIMLTGDNSHTAQAIAAQTGISDARGDQLPEDKLKVIEELSQRHGVRGV 663 + +LRALG + ++MLTGD A I + G D +Q P KL I++ S R GV Sbjct: 418 LRQLRALGAKRIVMLTGDRRDVAMTIGSSLGFDDVMPEQSPASKLAAIKQAS-REGV--T 474 Query: 664 GMVGDGINDAPALARADIGFAMGAAGTDTALETADVALMDDDLRKIPDFIRLSRKAHAVL 723 MVGDGINDAPALA ADIG AMGA G A E+A V L+ D L ++ +R++++A + Sbjct: 475 MMVGDGINDAPALAAADIGVAMGARGAAAAAESAQVVLLVDRLDRLAFAVRMAQQARKIA 534 Query: 724 VQNISIALGIKAVFLGLALVG----MSSLWMAVFADMGASLIVVFNGLRLV 770 +Q++ +G+ + A G ++ + F D+ V+ N LR++ Sbjct: 535 LQSVVAGMGLSGCAMLAAAFGYLPPVAGAILQEFIDVA----VILNALRVL 581 >gi|300312503|ref|YP_003776595.1| cation transport P-type ATPase [Herbaspirillum seropedicae SmR1] Length = 839 Score = 196 bits (497), Expect = 9e-51, Method: Compositional matrix adjust. Identities = 163/502 (32%), Positives = 251/502 (50%), Gaps = 36/502 (7%) Query: 292 DTVRVAPGERVPLDGLVLSGSTSINQAPITGESIPVQKQAGDPVFAGTINEQGAFEMRVT 351 D + PG+ + D +L G+ + A ++GES P Q G G +N +RV Sbjct: 346 DVILARPGDTIAADACLLEGAGEFDLALLSGESRPQSFQIGQEAPGGAVNLSQPVFLRVV 405 Query: 352 AVQGDSTLARIVKSVQQA-QGQRAPTQRFVDNFARYYIPAVVVMAVLVALLPPLLAGAAF 410 G+STLA + + +QA QG+ A Q + D A ++ VALL LLAGA F Sbjct: 406 RATGESTLAALTRLAEQAGQGKPALAQ-WADVVASRFV---------VALL--LLAGATF 453 Query: 411 QPW--------LYKALVLLVIACPCALVISTPVTIVSGLASAARHGILVKGGVYLEQGRQ 462 W A+ +LV++CPCAL ++TP + + R G L+ G LE ++ Sbjct: 454 LAWQLIDPTRAWSTAIAVLVVSCPCALSLATPSALAAATDRLLRAGTLIVRGNVLETLQR 513 Query: 463 LRALALDKTGTLTFGKPRVTDVVSLQAQADQQALLQLAVSLAARSDHPVSRAI---AAQE 519 + DKTGTLT G+P++T +L + D Q L +A ++ S HP+++A+ A+ Sbjct: 514 ADTIVFDKTGTLTQGRPQLT---ALWSDVDCQRSLAIAAAMERGSLHPLAKALVEEGARR 570 Query: 520 EIATVEVSEFAALAGRGVQGCINGQLLHLGNHRLVHEL-GQCSPELEARLQAFEKQGKTT 578 ++ +EV + ++ G G+QG I G L +G+ V L G P + LQ+ + Sbjct: 571 QVDALEVEQLTSVTGAGMQGMIEGDLYRIGSRSFVAGLAGTALPPV--LLQSAASGAGSV 628 Query: 579 TVLCLDGQPQLLVAVADTVRPSSRQAVAELRALGVEVIMLTGDNSHTAQAIAAQTGISDA 638 + DG +AD +R + VA RALG+ I+L+GD +AA GIS+ Sbjct: 629 YLGSSDGWLARF-DLADALRTDAVATVAAFRALGLRTILLSGDQPEVCAEVAAAAGISEV 687 Query: 639 RGDQLPEDKLKVIEELSQRHGVRGVGMVGDGINDAPALARADIGFAMGAAGTDTALETAD 698 + P+ KL + L QR G V +VGDGINDA L D+ FAMG G A +AD Sbjct: 688 QAACTPQGKLDYVRNL-QRDGAV-VAIVGDGINDAAMLRAGDVSFAMG-KGAALAQVSAD 744 Query: 699 VALMDDDLRKIPDFIRLSRKAHAVLVQNISIALGIKAVFLGLALVGMSSLWMAVFADMGA 758 +M D L+ + D ++++ ++ QN+ A + + A G+ WM+ + Sbjct: 745 AVIMSDRLQAVADCAAMAQRTMHIVRQNLVWAATYNFLAIPAAAFGLLDPWMSAVGMSLS 804 Query: 759 SLIVVFNGLRLVRGLRQRKAAA 780 SL+VV N LRL R RQ ++AA Sbjct: 805 SLLVVGNALRLSR--RQVRSAA 824 >gi|300313072|ref|YP_003777164.1| high-affinity K+ transport system ATPase [Herbaspirillum seropedicae SmR1] Length = 730 Score = 113 bits (283), Expect = 6e-26, Method: Compositional matrix adjust. Identities = 132/475 (27%), Positives = 225/475 (47%), Gaps = 67/475 (14%) Query: 292 DTVRVAPGERVPLDGLVLSGSTSINQAPITGESIPVQKQAG---DPVFAGTINEQGAFEM 348 D V + G+ VP D V+ G +++++ ITGES PV +++G V GT + Sbjct: 160 DLVLIEAGDTVPADAEVIEGVATVDESAITGESAPVIRESGGDFSAVTGGTRVLSDWIVV 219 Query: 349 RVTAVQGDSTLARIVKSVQQAQGQRAPTQRFVDNFARYYIPAVVVMAVLVALLP----PL 404 RV+ G++ L R++ V+ A+ Q+ P + + +V + V V LLP + Sbjct: 220 RVSVNPGEAFLDRMIAMVEGARRQKTPNELALTLLLVAL--TLVFLLVTVTLLPFSIFSV 277 Query: 405 LAGAAFQPWLYKALV-LLVIACPCALVISTPVTIVSGLASAARHGILVKGGVYLEQGRQL 463 A + P AL+ LLV P + V+G++ + ++ G +E + Sbjct: 278 NAAGSGSPVSVTALIALLVCLIPTTIGGLLSAIGVAGMSRMMQANVIATSGRAVEAAGDV 337 Query: 464 RALALDKTGTLTFGK---------PRVT-----DVVSLQAQADQQALLQLAVSLAARSDH 509 L LDKTGT+T G P VT DV L + AD+ + V LA Sbjct: 338 DVLLLDKTGTITLGNRQAAAFMPAPGVTERELADVAQLASLADETPEGRSIVVLAK---- 393 Query: 510 PVSRAIAAQEEIATVEVSEFAALAGRGVQGCINGQLLHLGNHRL-------VHELGQCS- 561 +R + E+A + +EF + Q ++G + LG+ R+ + +L + + Sbjct: 394 --NRFNLREREMANLG-AEFIPFSA---QTRVSG--IDLGHRRIRKGAADAIRKLTRAAD 445 Query: 562 ----PELEARLQAFEKQGKTTTV---------LCLDGQPQLL---VAVADTVRPSSRQAV 605 ++E+ +++ ++G T V + G P + V + D V+ ++ Sbjct: 446 QRLPAQVESDIESIARRGGTPLVVIEWHAEAAMINQGAPIRILGTVELKDIVKGGIKERF 505 Query: 606 AELRALGVEVIMLTGDNSHTAQAIAAQTGISDARGDQLPEDKLKVIEELSQRHGVRG--V 663 ELR +G++ +M+TGDN TA AIAA+ G+ D + PE KL +I ++H G V Sbjct: 506 GELRRMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEQKLALI----RQHQAEGKLV 561 Query: 664 GMVGDGINDAPALARADIGFAMGAAGTDTALETADVALMDDDLRKIPDFIRLSRK 718 M GDG NDAPALA+AD+ AM +GT A E ++ +D + K+ + + + ++ Sbjct: 562 AMTGDGTNDAPALAQADVAVAMN-SGTQAAKEAGNMVDLDSNPTKLIEIVEIGKQ 615 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.134 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,994,686 Number of extensions: 77998 Number of successful extensions: 391 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 366 Number of HSP's successfully gapped: 8 Length of query: 780 Length of database: 1,619,870 Length adjustment: 93 Effective length of query: 687 Effective length of database: 1,179,515 Effective search space: 810326805 Effective search space used: 810326805 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 134 (56.2 bits)