BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0029.0123.p (570 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310916|ref|YP_003775008.1| phospholipase C protein [Herbas... 1112 0.0 gi|300311919|ref|YP_003776011.1| non-hemolytic phospholipase C (... 85 2e-17 >gi|300310916|ref|YP_003775008.1| phospholipase C protein [Herbaspirillum seropedicae SmR1] Length = 558 Score = 1112 bits (2876), Expect = 0.0, Method: Compositional matrix adjust. Identities = 539/546 (98%), Positives = 540/546 (98%) Query: 25 PSRRRFLGGMAALGAGSALAGCAPEGQGSAPPPSGNPAEAAALDRALREQVKTIVVIYAE 84 P+ LGGMAALGAGSALAGCA EGQGSAPPPSGNPAEAAALDRALREQVKTIVVIYAE Sbjct: 13 PAAAASLGGMAALGAGSALAGCATEGQGSAPPPSGNPAEAAALDRALREQVKTIVVIYAE 72 Query: 85 NRSFNNLFADFPGLEKPLSALKPSDYQQRDRDGSLLKQLPPAWSGVVNQEQTVEGVTYEA 144 NRSFNNLFADFPGLEKPLSALKPSDYQQRDRDGSLLKQLPPAWSGVVNQEQTVEGVTYEA Sbjct: 73 NRSFNNLFADFPGLEKPLSALKPSDYQQRDRDGSLLKQLPPAWSGVVNQEQTVEGVTYEA 132 Query: 145 GKQYQTNLPNAPYALKGPQGEALPLGLVTRDLWHVFYQNQMQINGGKNDGFVAWADSGGL 204 GKQYQTNLPNAPYALKGPQGEALPLGLVTRDLWHVFYQNQMQINGGKNDGFVAWADSGGL Sbjct: 133 GKQYQTNLPNAPYALKGPQGEALPLGLVTRDLWHVFYQNQMQINGGKNDGFVAWADSGGL 192 Query: 205 TMGHYADTRYHLRLWDVAREFVLCDNFFQGAFGGSFLNHQYLICATAPYYPNVMNSVSRT 264 TMGHYADTRYHLRLWDVAREFVLCDNFFQGAFGGSFLNHQYLICATAPYYPNVMNSVSRT Sbjct: 193 TMGHYADTRYHLRLWDVAREFVLCDNFFQGAFGGSFLNHQYLICATAPYYPNVMNSVSRT 252 Query: 265 QVAKTKNNDPLSPELVPAEGSPASALQGPPKFGPSALTPDGYAVNTMAPPYWPTWSRDRN 324 QVAKTKNNDPLSPELVPAEGSP SALQGPPKFGPSALTPDGYAVNTMAPPYWPTWSRDRN Sbjct: 253 QVAKTKNNDPLSPELVPAEGSPTSALQGPPKFGPSALTPDGYAVNTMAPPYWPTWSRDRN 312 Query: 325 NPAYAEPNQPSVLVPQTHEHIGDKLNKKGIDWAWYAGAWQATLDEFKDSKGIPKIPNFQY 384 NPAYAEPNQPSVLVPQTHEHIGDKLNKKGIDWAWYAGAWQATLDEFKDSKGIPKIPNFQY Sbjct: 313 NPAYAEPNQPSVLVPQTHEHIGDKLNKKGIDWAWYAGAWQATLDEFKDSKGIPKIPNFQY 372 Query: 385 HHQPFNYFKNLGPENPAERNKRLRDGGLGDEARTNRFLADVEAGRLPPVTFYKPQGNLNL 444 HHQPFNYFKNLGPENPAERNKRLRDGGLGDEARTNRFLADVEAGRLPPVTFYKPQGNLNL Sbjct: 373 HHQPFNYFKNLGPENPAERNKRLRDGGLGDEARTNRFLADVEAGRLPPVTFYKPQGNLNL 432 Query: 445 HAGYADIASGDRHIAHIIKSLQASPQWKNMVVVVTFDENGGWWDHVAPPKGDRWGPGTRV 504 HAGYADIASGDRHIAHIIKSLQASPQWKNMVVVVTFDENGGWWDHVAPPKGDRWGPGTRV Sbjct: 433 HAGYADIASGDRHIAHIIKSLQASPQWKNMVVVVTFDENGGWWDHVAPPKGDRWGPGTRV 492 Query: 505 PALVVSPFARKGTVDHTVYDTASILRLITRTFDLEKLDGLKQRDEAMIARGQQPMGDLTN 564 PALVVSPFARKGTVDHTVYDTASILRLITRTFDLEKLDGLKQRDEAMIARGQQPMGDLTN Sbjct: 493 PALVVSPFARKGTVDHTVYDTASILRLITRTFDLEKLDGLKQRDEAMIARGQQPMGDLTN 552 Query: 565 ALQLKV 570 ALQLKV Sbjct: 553 ALQLKV 558 >gi|300311919|ref|YP_003776011.1| non-hemolytic phospholipase C (phosphatidylcholine choline phospho hydrolase) signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 696 Score = 84.7 bits (208), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 118/509 (23%), Positives = 185/509 (36%), Gaps = 155/509 (30%) Query: 73 EQVKTIVVIYAENRSFNNLFADFPGLE-------KPLSALKPSDYQQRDRDGSLLKQLPP 125 + VK +V++ ENRSF++ F G+ +PL P + QRD G L+ LP Sbjct: 48 KDVKHVVILMQENRSFDHYFGCLRGVRGFADPIPRPLPDGSPV-FHQRDGQGRLV--LP- 103 Query: 126 AWSGVVNQEQTVEGVTYEAGKQYQTNLPNAPYALKGPQGEALPLGLVTRDLWHVFYQNQM 185 +E T AG+ NLP H + Sbjct: 104 ---------YRMEMATTSAGRA-GGNLP------------------------HAWKDQHA 129 Query: 186 QINGGKNDGFVAWADSGGLTMGHYA--DTRYHLRLWDVAREFVLCDNFFQGAFGGSFLNH 243 N G D ++ A +TM ++ D +H L D F LCD + G + Sbjct: 130 AWNRGDYDKWIE-AKKDTVTMAYFTREDLPFHYALADA---FTLCDAYHCSVLGST---- 181 Query: 244 QYLICATAPYYPNVMNSVSRTQVAKTKNNDPLS--PELVPAEGSPASALQGPPKFGPSAL 301 PN + +S + + P++ P G P G FG Sbjct: 182 ----------NPNRFHLMSGGIDLQGRAGGPMTFQPRTGSCSGVPGERRSGADSFG---- 227 Query: 302 TPDGYAVNTMAPPYWPTWSRDRNNPAYAEPNQPSVLVPQTHEHIGDKLNKKGIDWAWYAG 361 W T+ ++L + G+ W Y Sbjct: 228 --------------WTTYP--------------------------ERLEQHGVSWKVY-- 245 Query: 362 AWQATLDEFKDSKGIPKIPNFQYHHQPFNYFKNLGPENPAERNKRLRDGGLGDEARTNRF 421 Q T + KD P +F F ++N P++P + K L D AR Sbjct: 246 --QGTEELHKDHGDYPS--DFNVLQYFFAQYQNAAPDSPLWQ-KACSKFTLADLAR---- 296 Query: 422 LADVEAGRLPPVTFYKPQGNLNLHAGYADIASGDRHIAHIIKSLQASPQ-WKNMVVVVTF 480 DV +G+LP V++ P L+ G +I+ ++ L A+P W + V++V + Sbjct: 297 --DVNSGKLPQVSWLMPP-KLSCEHPDRTPGYGAHYISQVLDILTANPALWSSTVLLVNY 353 Query: 481 DENGGWWDHVAPP---------------------KGDRWGP--------GTRVPALVVSP 511 DEN G++DHV PP KGD P G RVP +V+SP Sbjct: 354 DENDGFFDHVVPPTPPLHAGQGLSTVETSAEFHRKGDYVNPADDLPAGLGARVPMMVISP 413 Query: 512 FARKGTVDHTVYDTASILRLITRTFDLEK 540 +++ G V V+D S++R + + FD+ + Sbjct: 414 WSKGGYVCSEVFDHTSVIRFLEQVFDVHE 442 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.135 0.422 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,976,157 Number of extensions: 88622 Number of successful extensions: 247 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 246 Number of HSP's successfully gapped: 2 Length of query: 570 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 480 Effective length of database: 1,193,720 Effective search space: 572985600 Effective search space used: 572985600 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 133 (55.8 bits)