BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0029.0122.p (336 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310915|ref|YP_003775007.1| alkanesulfonates ABC transporte... 660 0.0 gi|300310977|ref|YP_003775069.1| alkanesulfonates ABC transporte... 287 1e-78 gi|300309984|ref|YP_003774076.1| nitrate/sulfonate/bicarbonate A... 245 4e-66 gi|300313870|ref|YP_003777962.1| nitrate/sulfonate/bicarbonate A... 225 3e-60 gi|300309986|ref|YP_003774078.1| nitrate/sulfonate/bicarbonate A... 106 3e-24 >gi|300310915|ref|YP_003775007.1| alkanesulfonates ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 336 Score = 660 bits (1703), Expect = 0.0, Method: Compositional matrix adjust. Identities = 335/336 (99%), Positives = 335/336 (99%) Query: 1 MPHDQAQRNKAIQTAVRSPRRRGLIAGAVTLGAAAAAGASLPAWVHAAAPRQLRVGYQKG 60 MPHDQAQRNKAIQTAVRSPRRR LIAGAVTLGAAAAAGASLPAWVHAAAPRQLRVGYQKG Sbjct: 1 MPHDQAQRNKAIQTAVRSPRRRRLIAGAVTLGAAAAAGASLPAWVHAAAPRQLRVGYQKG 60 Query: 61 ALSLIRAHGVLEQRLAPLGATVKWVEFSAGPVQLEALGAGSIDFGDVGEAPPIFAQAAGT 120 ALSLIRAHGVLEQRLAPLGATVKWVEFSAGPVQLEALGAGSIDFGDVGEAPPIFAQAAGT Sbjct: 61 ALSLIRAHGVLEQRLAPLGATVKWVEFSAGPVQLEALGAGSIDFGDVGEAPPIFAQAAGT 120 Query: 121 PLVYVGATVPRPYVEAVLVKDDSELQRPQQLKGKRIALNKGSNVHYFLVKLLEKHGLKYG 180 PLVYVGATVPRPYVEAVLVKDDSELQRPQQLKGKRIALNKGSNVHYFLVKLLEKHGLKYG Sbjct: 121 PLVYVGATVPRPYVEAVLVKDDSELQRPQQLKGKRIALNKGSNVHYFLVKLLEKHGLKYG 180 Query: 181 DVTPVWLAPADARAAFERGAVDAWAIWEPYLAAAQQGLKARILADADGVVKNRAYYFSTR 240 DVTPVWLAPADARAAFERGAVDAWAIWEPYLAAAQQGLKARILADADGVVKNRAYYFSTR Sbjct: 181 DVTPVWLAPADARAAFERGAVDAWAIWEPYLAAAQQGLKARILADADGVVKNRAYYFSTR 240 Query: 241 DYARDHADLLRIALEELGKIDAWAQARPDEYASELAGILGLPRSVLDVVVRRYHYGTLPI 300 DYARDHADLLRIALEELGKIDAWAQARPDEYASELAGILGLPRSVLDVVVRRYHYGTLPI Sbjct: 241 DYARDHADLLRIALEELGKIDAWAQARPDEYASELAGILGLPRSVLDVVVRRYHYGTLPI 300 Query: 301 TRAILDEQQQIADTFLDLRLIPRKISLLEAAPPWLA 336 TRAILDEQQQIADTFLDLRLIPRKISLLEAAPPWLA Sbjct: 301 TRAILDEQQQIADTFLDLRLIPRKISLLEAAPPWLA 336 >gi|300310977|ref|YP_003775069.1| alkanesulfonates ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 327 Score = 287 bits (734), Expect = 1e-78, Method: Compositional matrix adjust. Identities = 153/327 (46%), Positives = 214/327 (65%), Gaps = 7/327 (2%) Query: 6 AQRNKAIQTAVRSPRRRGLIAGAVTLGAAAAAGASLPAWVHAAAPRQLRVGYQKGA--LS 63 A +NK + A R +R A+ LGA A + P V A + LR+GYQK A L Sbjct: 3 ANKNKTVHQARRRAIQR---LSAIALGALTIALGASP--VQAQETKLLRIGYQKAANTLV 57 Query: 64 LIRAHGVLEQRLAPLGATVKWVEFSAGPVQLEALGAGSIDFGDVGEAPPIFAQAAGTPLV 123 L++AHG LE+RL PLG VKW EF+AGP LEAL GS+DFG VGEAPP+FAQAAG V Sbjct: 58 LLKAHGTLEKRLQPLGVEVKWAEFAAGPQLLEALNVGSVDFGYVGEAPPVFAQAAGADFV 117 Query: 124 YVGATVPRPYVEAVLVKDDSELQRPQQLKGKRIALNKGSNVHYFLVKLLEKHGLKYGDVT 183 Y +P P E +LV+ +S ++ +LKGK+IA NKGS+VH+ +V LL+K GL+Y D+ Sbjct: 118 YTAYEIPTPDAEGLLVQKNSPIKSVAELKGKKIAFNKGSDVHWLVVALLKKAGLQYSDIQ 177 Query: 184 PVWLAPADARAAFERGAVDAWAIWEPYLAAAQQGLKARILADADGVVKNRAYYFSTRDYA 243 PV+LAPADARAAFERGAVDAWAIW+P+ AAA++ + AR LA G V + ++ S R +A Sbjct: 178 PVYLAPADARAAFERGAVDAWAIWDPFQAAAEKQVGARRLATGVGAVNHHQFFLSARPFA 237 Query: 244 RDHADLLRIALEELGKIDAWAQARPDEYASELAGILGLPRSVLDVVVRRYHYGTLPITRA 303 + + +I LEE+ + W + + A++LA I GL +++ ++RY + P+ Sbjct: 238 AKNPQVEKILLEEISREGEWVRTHTAQAAAQLAPIQGLDADIIETGLKRYAHVYKPVDAQ 297 Query: 304 ILDEQQQIADTFLDLRLIPRKISLLEA 330 +L EQQ+IAD F +L+LIP+ + + +A Sbjct: 298 VLAEQQKIADAFYELKLIPKPLKVSDA 324 >gi|300309984|ref|YP_003774076.1| nitrate/sulfonate/bicarbonate ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 328 Score = 245 bits (625), Expect = 4e-66, Method: Compositional matrix adjust. Identities = 137/318 (43%), Positives = 194/318 (61%), Gaps = 9/318 (2%) Query: 18 SPRRRGL-IAGAVTLGAAAAAGASLPAWVHAAAPRQLRVGYQKGA--LSLIRAHGVLEQR 74 S RRR L A A+ LG+A A PA V A + +R+GYQK + L++++A+G LE+ Sbjct: 8 STRRRLLHAATALGLGSALA-----PASVFAQGNKAIRIGYQKSSTLLTILKANGTLEKL 62 Query: 75 LAPLGATVKWVEFSAGPVQLEALGAGSIDFG-DVGEAPPIFAQAAGTPLVYVGATVPRPY 133 LAP GA + W EF++G LEAL G +D DV + P+FAQAAG L YV P P Sbjct: 63 LAPTGAKISWHEFASGLPLLEALNVGGVDLSADVADTVPVFAQAAGAKLAYVAQESPSPA 122 Query: 134 VEAVLVKDDSELQRPQQLKGKRIALNKGSNVHYFLVKLLEKHGLKYGDVTPVWLAPADAR 193 +A++V+ DS L++ LKGK+IA+ K + VHY L+ LEK GLK+ DV +L+PAD R Sbjct: 123 AQAIVVRADSVLRKASDLKGKKIAVTKAAGVHYLLIATLEKAGLKFSDVDAAYLSPADGR 182 Query: 194 AAFERGAVDAWAIWEPYLAAAQQGLKARILADADGVVKNRAYYFSTRDYARDHADLLRIA 253 AAFERG+VDAW W+P+LA Q+ + RIL+D GV + YY ++ +A+ ++LR+ Sbjct: 183 AAFERGSVDAWVTWDPFLAGVQRQSQVRILSDGRGVADYQRYYLASASFAQARPEVLRVV 242 Query: 254 LEELGKIDAWAQARPDEYASELAGILGLPRSVLDVVVRRYHYGTLPITRAILDEQQQIAD 313 +EL K W + P E A+ LA + GL +++ R Y + L EQQ+IAD Sbjct: 243 FDELQKTGHWVKQHPKEAAAFLAPLWGLDADTIELANSRRSYEVRAVRPEALGEQQRIAD 302 Query: 314 TFLDLRLIPRKISLLEAA 331 TF L+PRKI+ + A Sbjct: 303 TFFAAGLLPRKINTNDVA 320 >gi|300313870|ref|YP_003777962.1| nitrate/sulfonate/bicarbonate ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 323 Score = 225 bits (574), Expect = 3e-60, Method: Compositional matrix adjust. Identities = 125/304 (41%), Positives = 175/304 (57%), Gaps = 2/304 (0%) Query: 32 GAAAAAGASLPAWVHAAAPRQLRVGYQK-GALSLIRAHGVLEQRLAPLGATVKWVEFSAG 90 G AA A AS V AAA + LR+GYQK L++++ G LEQ L P G +VKW EF+AG Sbjct: 16 GLAAGALASGTPLVRAAADKVLRIGYQKFNTLNILKGSGKLEQALQPQGWSVKWFEFAAG 75 Query: 91 PVQLEALGAGSIDFGDVGEAPPIFAQAAGTPLVYVGATVPRPYVEAVLVKDDSELQRPQQ 150 P EAL A +ID G + P +FA A+G VY+GA P P + V +S ++ Sbjct: 76 PQLTEALNANAIDLGHAADTPSVFANASGVNAVYLGAEQPYPKGIGIFVAQESPIRSVAD 135 Query: 151 LKGKRIALNKGSNVHYFLVKLLEKHGLKYGDVTPVWLAP-ADARAAFERGAVDAWAIWEP 209 LKGK++A +G NV + L+K LE+ GL Y D+ PV++ AD RAAF+ G VDA +W+P Sbjct: 136 LKGKKVATGRGWNVQFLLLKALEEVGLSYRDIEPVYVTNAADVRAAFQSGNVDAATLWDP 195 Query: 210 YLAAAQQGLKARILADADGVVKNRAYYFSTRDYARDHADLLRIALEELGKIDAWAQARPD 269 +LA + + RIL D G+ NR ++ + + H D++R EL K + W Q+ P Sbjct: 196 FLAGQELATRPRILRDGTGLSNNRTFHLAVPSFVDRHKDIVRTVFAELKKTNQWTQSHPQ 255 Query: 270 EYASELAGILGLPRSVLDVVVRRYHYGTLPITRAILDEQQQIADTFLDLRLIPRKISLLE 329 E A LA LG+ VL + R +Y T+ + I+ EQQQIAD FL L LI + + + Sbjct: 256 ETAELLAPQLGVDPKVLRLATERRNYTTVAVDAGIVAEQQQIADAFLKLGLIKNPVRVQD 315 Query: 330 AAPP 333 P Sbjct: 316 KVYP 319 >gi|300309986|ref|YP_003774078.1| nitrate/sulfonate/bicarbonate ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 328 Score = 106 bits (264), Expect = 3e-24, Method: Compositional matrix adjust. Identities = 99/321 (30%), Positives = 152/321 (47%), Gaps = 19/321 (5%) Query: 20 RRRGLIAGAVTLGAAAAAGASLPAWVHAAAPRQ---LRVGYQKGALSLIRAHGVLEQRLA 76 RR L LG A G +P AA P + L Y ++R G LE+ L Sbjct: 3 RRDFLRLAPAALGVTALGG--IPLLSSAAQPPKVIRLDYAYYSPPSLVLRKFGWLEEDLK 60 Query: 77 PLGATVKWVEFSAGPVQLEALGAGSIDFGDVGEAPPIFAQAAGTPL--VYVGATVPRPYV 134 G VKWV LE L + S+DFG + A+A G P+ VY+ + RP Sbjct: 61 AQGTEVKWVLSQGSNRALEYLNSDSVDFGSTAGLAALLARANGNPVKAVYIYS---RPEW 117 Query: 135 EAVLVKDDSELQRPQQLKGKRIALNKGSNVHYFLVKLLEKHGLKYGDVTPVWLAPADARA 194 A+ V +S ++ +LKGK+IA KG++ + FL++ L ++GLK DV V L AD RA Sbjct: 118 TALAVGKESPVKSINELKGKKIAATKGTDPYLFLLRALHENGLKKSDVEIVHLQHADGRA 177 Query: 195 AFERGAVDAWAIWEPYLAAAQQGLKARILADADGVVKNRAYYFSTRD-YARDHADLLRIA 253 A E+G V AWA +P+LAA++ +R++ V N + + D +A H D ++ Sbjct: 178 ALEQGRVAAWAGLDPHLAASELEAGSRLI--YRNVNFNTYGFLNVSDSFAARHPDQVKRV 235 Query: 254 LEELGKIDAWAQARPDEYASELAGILGLPRSVLDVVVRRYHYGTLPITRAILDEQQQIAD 313 + + +W A PDE + L+ L V + ++R + I R ++ + A Sbjct: 236 IAAYERARSWIIAHPDETVALLSEESKLSAPVARLQLKRNDFSQPQIGREHIEALRAAAP 295 Query: 314 TFLDLRLI------PRKISLL 328 L+ L+ PR I+ L Sbjct: 296 ILLEEDLVKSGTDLPRTINAL 316 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.136 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,071,565 Number of extensions: 46790 Number of successful extensions: 194 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 187 Number of HSP's successfully gapped: 7 Length of query: 336 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 250 Effective length of database: 1,212,660 Effective search space: 303165000 Effective search space used: 303165000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)