BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0029.0121.p (572 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310913|ref|YP_003775005.1| chloride channel EriC protein [... 1100 0.0 gi|300311435|ref|YP_003775527.1| chloride channel protein [Herba... 112 7e-26 gi|300311782|ref|YP_003775874.1| chloride channel protein [Herba... 65 2e-11 >gi|300310913|ref|YP_003775005.1| chloride channel EriC protein [Herbaspirillum seropedicae SmR1] Length = 572 Score = 1100 bits (2846), Expect = 0.0, Method: Compositional matrix adjust. Identities = 570/572 (99%), Positives = 570/572 (99%) Query: 1 MKHKRDYTADRRLPYLCAIALPIGLLSTGAAWLLLKLIALFTNLFYFQRLSLAASTPATH 60 MKHKRDYTADRRLPYLCAIALPIGLLSTGAAWLLLKLIALFTNLFYFQRLSLAASTPATH Sbjct: 1 MKHKRDYTADRRLPYLCAIALPIGLLSTGAAWLLLKLIALFTNLFYFQRLSLAASTPATH 60 Query: 61 TLGWIAALLPIAGGLIVGLMARYGSDKIRGHGIPEAIEAILFGKSRMSPKVALLKPLSSG 120 TLGWIAALLPIAGGLIVGLMARYGSDKIRGHGIPEAIEAILFGKSRMSPKVALLKPLSSG Sbjct: 61 TLGWIAALLPIAGGLIVGLMARYGSDKIRGHGIPEAIEAILFGKSRMSPKVALLKPLSSG 120 Query: 121 IVIGSGGPFGAEGPIIMTGGSIGSLLAQLLHLTAAERKTLLVAGACAGMTAIFGTPLAAV 180 IVIGSGGPFGAEGPIIMTGGSIGSLLAQLLHLTAAERKTLLVAGACAGMTAIFGTPLAAV Sbjct: 121 IVIGSGGPFGAEGPIIMTGGSIGSLLAQLLHLTAAERKTLLVAGACAGMTAIFGTPLAAV 180 Query: 181 LLAVELLLFELKPRSLLPVALACAVAGFVRPWLVEAGPLFPLDVAAPTPVALLSCLVAGL 240 LLAVELLLFELKPRSLLPVALACAVAGFVRPWLVEAGPLFPLDVAAPTPVALLSCLVAGL Sbjct: 181 LLAVELLLFELKPRSLLPVALACAVAGFVRPWLVEAGPLFPLDVAAPTPVALLSCLVAGL 240 Query: 241 ASGVLAAALTLALYRIEDGFHRLRLHWMWWPALGGIAVGLGGLLQPRALGVGYDVIGDLL 300 ASGVLAAALTLALYRIEDGFHRLRLHWMWWPALGGIAVGLGGLLQPRALGVGYDVIGDLL Sbjct: 241 ASGVLAAALTLALYRIEDGFHRLRLHWMWWPALGGIAVGLGGLLQPRALGVGYDVIGDLL 300 Query: 301 HQHMLLSAALTLLLVKAVIWLLALGSGTSGGVLAPLLMLGAGLGLVLSPWLPGGSDGLWP 360 HQHMLLSAAL LLLVKAVIWLLALGSGTSGGVLAPLLMLGAGLGLVLSPWLPGGSDGLWP Sbjct: 301 HQHMLLSAALALLLVKAVIWLLALGSGTSGGVLAPLLMLGAGLGLVLSPWLPGGSDGLWP 360 Query: 361 LVCMAGVLGSVLGAPLTAIVFAFGLTHASQALLPLIATTAVAYGVNVLLMKRSIMTEKIA 420 LVCMAGVLGSVLGAPLTAIVFAFGLTHASQALLPLIATTAVAYGVNVLLMKRSIMTEKIA Sbjct: 361 LVCMAGVLGSVLGAPLTAIVFAFGLTHASQALLPLIATTAVAYGVNVLLMKRSIMTEKIA 420 Query: 421 RRGLHVHREYGVDPLERMHVEELMTAAPQTLAGALPAAEALAWCRAQAHVHRYYPVTDGV 480 RRGLHVHREYGVDPLERMHVEELMTAAPQTLAGALPAAEALAWCRAQ HVHRYYPVTDGV Sbjct: 421 RRGLHVHREYGVDPLERMHVEELMTAAPQTLAGALPAAEALAWCRAQEHVHRYYPVTDGV 480 Query: 481 HLVGMLALRALEHADASQPCDTLAQGHEHYLLPHMSARSAAGMMARLGVARLPVVAGPER 540 HLVGMLALRALEHADASQPCDTLAQGHEHYLLPHMSARSAAGMMARLGVARLPVVAGPER Sbjct: 481 HLVGMLALRALEHADASQPCDTLAQGHEHYLLPHMSARSAAGMMARLGVARLPVVAGPER 540 Query: 541 RKLVGVLSLSDLARASAAHAEEESVRERLLGG 572 RKLVGVLSLSDLARASAAHAEEESVRERLLGG Sbjct: 541 RKLVGVLSLSDLARASAAHAEEESVRERLLGG 572 >gi|300311435|ref|YP_003775527.1| chloride channel protein [Herbaspirillum seropedicae SmR1] Length = 555 Score = 112 bits (281), Expect = 7e-26, Method: Compositional matrix adjust. Identities = 145/519 (27%), Positives = 231/519 (44%), Gaps = 46/519 (8%) Query: 64 WIAALLPIAGGLIVGLMARYGSDKIRGHGIPEAIEAILFGKSRMSPKVALLKPLSSGIVI 123 + LLP GG++ GL + G+G + +EA+ G + + LL+ +SS I Sbjct: 47 YTRVLLPTCGGVVAGLFLVWAKRTPAGNG-GDYMEAVAIGDGAIPVRNTLLRSISSLASI 105 Query: 124 GSGGPFGAEGPIIMTGGSIGSLLAQLLHLTAAERKTLLVAGACAGMTAIFGTPLAAVLLA 183 SGG G EGP++ SL+ + H ++ + L+ GA AG+T+ + P+A Sbjct: 106 ASGGSIGREGPMVQLSALCASLVGKFSHFPSSRLRLLVACGAAAGITSAYNAPIAGAFFI 165 Query: 184 VELLLFELKPRSLLPVALACAVAGFVRPWL-----VEAGPLFPLDVAAPTPVALLSCLVA 238 E++L L S PV +A VA L P FP ++ ++ LV Sbjct: 166 TEIVLGSLVMESFGPVVVASVVANITMRELPGYRAAYEMPYFP-EIGG---WEVMLFLVL 221 Query: 239 GLASGVLAAALTLALYRIEDGFHRLRLHWMWWPALGGIAVGLGGLLQPRALGVGYDVIGD 298 G+ +GVLA L + GF +LRL +GG+ VGL + P G GY ++ Sbjct: 222 GVLAGVLAPQFLRVLELGKHGFGKLRLPLPVRLGVGGLLVGLISVQVPEVWGNGYSLVNS 281 Query: 299 LLHQHMLLSAALTLLLVKAVIWLLALGSGTSGGVLAPLLMLGAGLGLVLSPWLPGGSDGL 358 LLH + A L +L+VK + + +GSG GG+ P L +GA +G + + L Sbjct: 282 LLHTSWVWQAVLMVLVVKVLATSITVGSGAVGGIFTPTLFVGAAVGYLFG----DAAQAL 337 Query: 359 WP----------LVCMAGVLGSVLGAPLTAIVFAFGLTHASQALLPLIATTAVAYGVNVL 408 P +V M L + APL AI+ F +T + Q +LPL+ + VAY Sbjct: 338 LPVHISQPFAYAMVGMGAFLAAASYAPLMAILMIFEMTLSYQVVLPLMLSCVVAY----- 392 Query: 409 LMKRSIMTEKIARRGLHVHREYGVDPLERM-----HVEELMTAAPQTLAGALPAAEALAW 463 ++ RS+ + L HR D ER+ H+ EL+ A +T+ + E ++ Sbjct: 393 VVARSVDGRSMYEITLKRHR----DNEERLRLRGTHMSELIRPA-ETVLHEEASLEEISA 447 Query: 464 CRAQAHVHRYYPVTDGVHLVGMLALRAL-------EHADASQPCDTLAQGHEHYLLPHMS 516 + V Y V +G++AL+ + + A D L H + P MS Sbjct: 448 LFLKYPVKYVYIVDGRQRYLGVVALQDITSWLLDKKEAAGRVARDFLRPHFLHEVTPDMS 507 Query: 517 ARSAAGMMARLGVARLPVVAGPERRKLVGVLSLSDLARA 555 A + RLPV+A + +L+GV+ + L A Sbjct: 508 LGEALQLFLDHQGERLPVIASADDPQLLGVVFKTSLLDA 546 >gi|300311782|ref|YP_003775874.1| chloride channel protein [Herbaspirillum seropedicae SmR1] Length = 442 Score = 64.7 bits (156), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 89/297 (29%), Positives = 136/297 (45%), Gaps = 34/297 (11%) Query: 64 WIAALLPIAGGLIVGLMARYGSDKIRGHG---IPEAIEAILFGKSRMSPKVALLKPLSSG 120 WI LLP+ GG VG + + + I E + RM+P V LL ++S Sbjct: 53 WIIWLLPL-GGFAVGWLYLHTGKPVEAGNNLLIDEIHDPRKVIPLRMAPLV-LLGTVASH 110 Query: 121 IVIGSGGPFGAEGPIIMTGGSIGSLLAQLLHLTAAERKTLLVAGACAGMTAIFGTPLAAV 180 + G G EG + GG++ L LL L +R+ LL+AG AG +++FGTP+A Sbjct: 111 LF---GASVGREGTAVQMGGALADQLTHLLRLRPEDRRILLMAGISAGFSSVFGTPMAGA 167 Query: 181 LLAVELL-LFELKPRSLLPVALACAVAGFVR-PWLVEAGPLFPLDVAAPTPVALLSCLVA 238 + +E+L + ++ ++ P +A A V W V + ++ +A P+AL S Sbjct: 168 IFGLEVLAIGRMRYEAIFPCFVAAIAADQVGLAWGVHH-THYAMNASA--PMALWSVAAV 224 Query: 239 -------GLASGVLAAAL-TL-ALYRIEDGFHRLRLHWMWWPALGGIAVGLG--GLLQPR 287 GLA + A + TL A + + LR P +GG V L + R Sbjct: 225 IAAGVVFGLAGMIFANSTHTLSAFMKRHVSYAPLR------PFIGGFIVALAVWAVGTHR 278 Query: 288 ALGVGYDVIGDLLHQHMLLSAALTLLLVKAVIWLLALGSGTSGGVLAPLLMLGAGLG 344 +G+G VI + Q + A L K V + +LGSG GG + PL +GA LG Sbjct: 279 YIGLGIPVIVEAFQQPL----APWDFLGKMVFTVTSLGSGFKGGEVTPLFYIGATLG 331 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.324 0.140 0.425 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,610,046 Number of extensions: 62896 Number of successful extensions: 432 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 422 Number of HSP's successfully gapped: 4 Length of query: 572 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 482 Effective length of database: 1,193,720 Effective search space: 575373040 Effective search space used: 575373040 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 133 (55.8 bits)