BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0029.0115.p (375 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310902|ref|YP_003774994.1| alkanesulfonate monooxygenase [... 757 0.0 gi|300310978|ref|YP_003775070.1| alkanesulfonate monooxygenase [... 454 e-129 gi|300310284|ref|YP_003774376.1| coenzyme F420-dependent N5,N10-... 141 8e-35 gi|300314031|ref|YP_003778123.1| coenzyme F420-dependent N5,N10-... 75 8e-15 gi|300313871|ref|YP_003777963.1| thiophene oxidase [Herbaspirill... 64 2e-11 >gi|300310902|ref|YP_003774994.1| alkanesulfonate monooxygenase [Herbaspirillum seropedicae SmR1] Length = 375 Score = 757 bits (1955), Expect = 0.0, Method: Compositional matrix adjust. Identities = 374/375 (99%), Positives = 374/375 (99%) Query: 1 MSLELFWFLPTSGDTRYLGKSDFGRAPTLEYMRQIAVTSETLGYDGLLIPTGASCLDPWV 60 MSLELFWFLPTSGDTRYLGKSDFGRAPTLEYMRQIAVTSETLGYDGLLIPTGASCLDPWV Sbjct: 1 MSLELFWFLPTSGDTRYLGKSDFGRAPTLEYMRQIAVTSETLGYDGLLIPTGASCLDPWV 60 Query: 61 VAASLVPVTQRIKLLVALRTSLGGPGPSARQAATLDQALNGRLLLNVVPGGDATELEADG 120 VAASLVPVTQRIKLLVALRTSLGGPGPSARQAATLDQALNGRLLLNVVPGGDATELEADG Sbjct: 61 VAASLVPVTQRIKLLVALRTSLGGPGPSARQAATLDQALNGRLLLNVVPGGDATELEADG 120 Query: 121 VFLKHDERYEAADEYLTIWRKLLQGETVNYEGKHFRIRNGQLFHPAVQQPYPPLYFGGSS 180 VFLKHDERYEAADEYLTIWRKLLQGETVNYEGKHFRIRNGQLFHPAVQQPYPPLYFGGSS Sbjct: 121 VFLKHDERYEAADEYLTIWRKLLQGETVNYEGKHFRIRNGQLFHPAVQQPYPPLYFGGSS 180 Query: 181 PAAHDLAAKHVDAYLTWGEPPAAVRQKIDDVRERAARQGRTVRFGVRLHAIVRETTAEAW 240 PAAHDLAAKHVDAYLTWGEPPAAVRQKIDDVRERAARQGRTVRFGVRLHAIVRETTAEAW Sbjct: 181 PAAHDLAAKHVDAYLTWGEPPAAVRQKIDDVRERAARQGRTVRFGVRLHAIVRETTAEAW 240 Query: 241 TAAERLISKLTDEDIARAQQNYARMDSVGQQRMAALHGGRRDKLEIAPNLWAGVGLVRGG 300 TAAERLISKLTDEDIARAQQNYARMDSVGQQRMAALHGGRRDKLEIAPNLWAGVGLVRGG Sbjct: 241 TAAERLISKLTDEDIARAQQNYARMDSVGQQRMAALHGGRRDKLEIAPNLWAGVGLVRGG 300 Query: 301 AGTSLVGDAATVAERLQEYADLGVDTFVLSGYPHLEEAIRFAELVFPLLGKNAVTLRDRA 360 AGTSLVGDA TVAERLQEYADLGVDTFVLSGYPHLEEAIRFAELVFPLLGKNAVTLRDRA Sbjct: 301 AGTSLVGDATTVAERLQEYADLGVDTFVLSGYPHLEEAIRFAELVFPLLGKNAVTLRDRA 360 Query: 361 QTGGAFDIRAAASAS 375 QTGGAFDIRAAASAS Sbjct: 361 QTGGAFDIRAAASAS 375 >gi|300310978|ref|YP_003775070.1| alkanesulfonate monooxygenase [Herbaspirillum seropedicae SmR1] Length = 388 Score = 454 bits (1169), Expect = e-129, Method: Compositional matrix adjust. Identities = 231/351 (65%), Positives = 278/351 (79%), Gaps = 4/351 (1%) Query: 3 LELFWFLPTSGDTRYLGKSDFGRAPTLEYMRQIAVTSETLGYDGLLIPTGASCLDPWVVA 62 + +FWF+PT GD+RYLG S+ RA + +YM+Q+AV ++TLGYDG+L+PTG SC D WV A Sbjct: 1 MNVFWFIPTHGDSRYLGTSEGARAVSHDYMKQVAVAADTLGYDGVLLPTGRSCEDAWVAA 60 Query: 63 ASLVPVTQRIKLLVALRTSLGGPGPSARQAATLDQALNGRLLLNVVPGGDATELEADGVF 122 ASL+ T+R+K LVA+R L P +AR AAT D+ GRLL+NVV GGD E E+DGVF Sbjct: 61 ASLIDATRRLKFLVAVRPGLMAPTLAARMAATFDRLSGGRLLINVVTGGDPVEQESDGVF 120 Query: 123 LKHDERYEAADEYLTIWRKLL----QGETVNYEGKHFRIRNGQLFHPAVQQPYPPLYFGG 178 H ERYE +DE+L IWR +L GE +++GKH ++ + +P VQQPYPPLYFGG Sbjct: 121 GSHAERYEISDEFLKIWRDVLTKTRSGEPTHFQGKHLSVKGAKALYPPVQQPYPPLYFGG 180 Query: 179 SSPAAHDLAAKHVDAYLTWGEPPAAVRQKIDDVRERAARQGRTVRFGVRLHAIVRETTAE 238 SS A DLAA+ VD YLTWGEPPAAV +KI VRERAA+ GRT+RFG+RLH IVRET Sbjct: 181 SSDEAIDLAAEQVDVYLTWGEPPAAVAEKIARVRERAAKAGRTLRFGIRLHVIVRETNEA 240 Query: 239 AWTAAERLISKLTDEDIARAQQNYARMDSVGQQRMAALHGGRRDKLEIAPNLWAGVGLVR 298 AW AA+ LIS+L DE IA+AQ N+ARMDS GQ+RMAALHGGRRDKLE++PNLWAGVGLVR Sbjct: 241 AWRAADELISRLDDETIAKAQANFARMDSEGQRRMAALHGGRRDKLEVSPNLWAGVGLVR 300 Query: 299 GGAGTSLVGDAATVAERLQEYADLGVDTFVLSGYPHLEEAIRFAELVFPLL 349 GGAGT+LVGD TVAERLQEYA LG+DTF+LSGYPHLEE+ RFAELVFPLL Sbjct: 301 GGAGTALVGDGPTVAERLQEYAALGIDTFILSGYPHLEESYRFAELVFPLL 351 >gi|300310284|ref|YP_003774376.1| coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase [Herbaspirillum seropedicae SmR1] Length = 385 Score = 141 bits (356), Expect = 8e-35, Method: Compositional matrix adjust. Identities = 106/331 (32%), Positives = 169/331 (51%), Gaps = 20/331 (6%) Query: 28 TLEYMRQIAVTSETLGYDGLLIPT------GASCLDPWV-VAASLVPVTQRIKLLVALRT 80 +LEY +++AVT+E G++ L GA V + +L+ T ++K+L A+ Sbjct: 42 SLEYNQRLAVTAEKAGFEYALSQIRFTAGYGAEYQHESVSFSQALLHATTKLKVLAAILP 101 Query: 81 SLGGPGPSARQAATLDQALNGRLLLNVVPGGDATELEADGV-FLKHDERYEAADEYLTIW 139 P A+Q AT+D NGR+ +NVV G E +A G +L+HDERY ++E++ Sbjct: 102 GPWHPAVVAKQIATIDHISNGRIAINVVSGWFKGEFQAIGEPWLEHDERYRRSNEFIRAL 161 Query: 140 RKLLQGETVNYEGKHFRIRNGQLFHPAVQQPYPPLYFGGSSPAAHDLAAKHVDAYLTWGE 199 + + + ++G +R N L VQ+P+P ++ GGSS AA D AA D Y T G Sbjct: 162 KGIWTQDDFTFKGDFYRFNNYTLSPKPVQKPHPEIFQGGSSRAARDNAASVSDWYFTNGN 221 Query: 200 PPAAVRQKIDDVRERAARQGRTVRFGVRLHAIVRETTAEAWTAAERLISKLTDEDIARAQ 259 ++ +IDD++ +AA+ G VR GV I R+T EA + +I D A + Sbjct: 222 TLEGLKAQIDDIQAKAAQNGHKVRIGVNAFVIARDTEEEAQAVLQDII------DHAHVE 275 Query: 260 QNYARMDSVGQQRMAALHGGRRDKLEIAPNLWAGVGLVRGGAGTSLVGDAATVAERLQEY 319 +A D+V Q A+ G + A + + + G T+L+G +AER+ Sbjct: 276 AVHAFGDAVKQAGKASPEG----EGNWAKSTFEDLVQYNDGFKTNLIGTPQQIAERIVAL 331 Query: 320 ADLGVDTFVLSGYPH-LEEAIRFAELVFPLL 349 +GVD VL+G+ H +EE F + V PL+ Sbjct: 332 KAIGVD-LVLTGFLHFIEEVEYFGQKVLPLV 361 >gi|300314031|ref|YP_003778123.1| coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase [Herbaspirillum seropedicae SmR1] Length = 459 Score = 75.1 bits (183), Expect = 8e-15, Method: Compositional matrix adjust. Identities = 83/347 (23%), Positives = 143/347 (41%), Gaps = 46/347 (13%) Query: 57 DPWVVAASLVPVTQRIKLLVALRTSLGGPGPSARQAATLDQALNGRLLLNVVPGGDATEL 116 DP ++ ++ VT+ + V + P AR+ +TLD GR+ N+V G + Sbjct: 85 DPLMLVPAMAAVTEHLGFGVTANLTYEAPFLFARRMSTLDHLSRGRVGWNIVTGYLDSAA 144 Query: 117 EADGVF--LKHDERYEAADEYLTIWRKLLQG----------------------ETVNYEG 152 A G+ L+HD+RY+ ADE++ + KL +G + + G Sbjct: 145 RAMGLHEQLEHDQRYDRADEFMEVVYKLWEGSWEDGAVLRDRARRVYADPAKVHKIAHHG 204 Query: 153 KHFRIRNGQLFHPAVQQPYPPLYFGGSSPAAHDLAAKHVDAYLTWGEPPAAVRQKIDDVR 212 ++ + L P+ Q+ P LY GSS A+H + + A R+ + +R Sbjct: 205 DYYHVEGIHLSEPSRQRT-PVLYQAGSSGRGQAFGARHAECVFISSQSKDATRKLVQSIR 263 Query: 213 ERAARQGR---TVRFGVRLHAIVRETTAEAWTAAERLISKLTDEDIARAQ---------Q 260 + A R+GR ++ + L + T +A R ++ + A Sbjct: 264 DGAVREGRDPDAIKIFMGLTVVTGRTEQQA-QERYREYARYASPEAGLAHYSAGTGIDFS 322 Query: 261 NYARMDSVGQQRMAALHGGRRDKLEIAPNL-WAGVGLVR----GGAGTSLVGDAATVAER 315 Y + + + A+ K+ P L W L++ GG S+VG VA+ Sbjct: 323 QYELDEPIRPAKTNAIES--LTKVVTQPELNWTKRKLLQQLELGGRYASIVGAPDRVADE 380 Query: 316 LQEY-ADLGVDTFVLSGYPHLEEAIRFAELVFPLLGKNAVTLRDRAQ 361 L+ + AD G+D + L+ E F ELV P+L + R+ AQ Sbjct: 381 LESWVADTGIDGYNLTRTVTPESFEDFVELVIPVLQERGSYKREYAQ 427 >gi|300313871|ref|YP_003777963.1| thiophene oxidase [Herbaspirillum seropedicae SmR1] Length = 433 Score = 63.9 bits (154), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 59/191 (30%), Positives = 83/191 (43%), Gaps = 26/191 (13%) Query: 53 ASCLDPWVVAASLVPVTQRIKLLVALRTSLGGPGPSARQAATLDQALNGRLLLNVVPGGD 112 A+ LDP + +L T I L T+ P AR+ ATLD GR NVV Sbjct: 77 ATRLDPLPILGALAAATSHIGLGATRSTTYSQPYSLAREFATLDHLSGGRAAWNVVTS-- 134 Query: 113 ATELEADGVFLK----HDERYEAADEYLTIWRKL----------LQGE---------TVN 149 + EAD LK HD RY+ ADE+L + KL LQ + ++ Sbjct: 135 VNQGEADNFGLKETLPHDVRYDRADEFLEVTHKLWHSWQPDALRLQQDRYADADKVAAID 194 Query: 150 YEGKHFRIRNGQLFHPAVQQPYPPLYFGGSSPAAHDLAAKHVDAYLTWGEPPAAVRQKID 209 ++G HF +R G L PA Q +P + GSS D AA+ + ++ Sbjct: 195 HQGAHFSVR-GPLNIPASPQGHPVIIQAGSSDRGQDFAARWAEVVFNIQPDLPRLQAFYR 253 Query: 210 DVRERAARQGR 220 D++ RA + GR Sbjct: 254 DLKNRAQQFGR 264 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.135 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,284,056 Number of extensions: 58608 Number of successful extensions: 178 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 172 Number of HSP's successfully gapped: 5 Length of query: 375 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 288 Effective length of database: 1,207,925 Effective search space: 347882400 Effective search space used: 347882400 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)