BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0029.0100.p (263 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310887|ref|YP_003774979.1| metal ion ABC transporter perip... 519 e-149 gi|300310325|ref|YP_003774417.1| metal ion ABC transporter perip... 215 2e-57 gi|300314030|ref|YP_003778122.1| metal ion ABC transporter perip... 198 3e-52 gi|300311120|ref|YP_003775212.1| metal ion ABC transporter perip... 183 1e-47 gi|300311637|ref|YP_003775729.1| metal ion ABC transporter perip... 120 1e-28 >gi|300310887|ref|YP_003774979.1| metal ion ABC transporter periplasmic component/surface antigen protein [Herbaspirillum seropedicae SmR1] Length = 263 Score = 519 bits (1337), Expect = e-149, Method: Compositional matrix adjust. Identities = 261/263 (99%), Positives = 262/263 (99%) Query: 1 MIRKLIPHLFAGAALAFSVGAHAADKLVIAATPVPHAEILEHIKPALAKEGIDLQIKVFT 60 MIRKLIPHLFAGAALAFSVGAHAADKLVIAATPVPHAEILEHIKPALAKEGIDLQ+KVFT Sbjct: 1 MIRKLIPHLFAGAALAFSVGAHAADKLVIAATPVPHAEILEHIKPALAKEGIDLQVKVFT 60 Query: 61 DYVQPAAQTNEKQVDGNFFLHQPYLDQFKKSHKNDIEVPVAKVHVEPFAAYSQKYKKIAD 120 DYVQPAAQTNEKQVDGNFFLHQPYLDQFKKSHKNDIEVPVAKVHVEPFAAYSQKYKKIAD Sbjct: 61 DYVQPAAQTNEKQVDGNFFLHQPYLDQFKKSHKNDIEVPVAKVHVEPFAAYSQKYKKIAD 120 Query: 121 IPNGATIAIPNDPSNSGRALLLLDRNGLIKLKDNTNISATQKDIVENPKKLKFRALEAAT 180 IPNGATIAIPNDPSNSGRALLLL RNGLIKLKDNTNISATQKDIVENPKKLKFRALEAAT Sbjct: 121 IPNGATIAIPNDPSNSGRALLLLARNGLIKLKDNTNISATQKDIVENPKKLKFRALEAAT 180 Query: 181 LPRVLNQVDVALINTNYALEAKLNPVKDSLFIEDANSPYANLLVAREDNKDSPAIKKLAA 240 LPRVLNQVDVALINTNYALEAKLNPVKDSLFIEDANSPYANLLVAREDNKDSPAIKKLAA Sbjct: 181 LPRVLNQVDVALINTNYALEAKLNPVKDSLFIEDANSPYANLLVAREDNKDSPAIKKLAA 240 Query: 241 ALNSPDVKKFIEEKYQGAIVPAF 263 ALNSPDVKKFIEEKYQGAIVPAF Sbjct: 241 ALNSPDVKKFIEEKYQGAIVPAF 263 >gi|300310325|ref|YP_003774417.1| metal ion ABC transporter periplasmic component/surface antigen protein [Herbaspirillum seropedicae SmR1] Length = 265 Score = 215 bits (548), Expect = 2e-57, Method: Compositional matrix adjust. Identities = 115/266 (43%), Positives = 162/266 (60%), Gaps = 4/266 (1%) Query: 1 MIRKLIPHLFAGAALA---FSVGAHAADKLVIAATPVPHAEILEHIKPALAKEGIDLQIK 57 M R+ + AG LA S A A D++ + T PHAEI+E +K L K+G+ +++ Sbjct: 1 MKRRQLIQFIAGLGLAAGLVSAPAFAQDQIKMGVTAGPHAEIMEQVKKLLEKDGVQMKVI 60 Query: 58 VFTDYVQPAAQTNEKQVDGNFFLHQPYLDQFKKSHKNDIEVPVAKVHVEPFAAYSQKYKK 117 FTDY+QP A +D N + HQPYLD K V V P YS+K K Sbjct: 61 EFTDYIQPNAALAAGDLDANSYQHQPYLDAQIKDRGYKF-VSVGSTITFPMGVYSKKIKS 119 Query: 118 IADIPNGATIAIPNDPSNSGRALLLLDRNGLIKLKDNTNISATQKDIVENPKKLKFRALE 177 + D+ GA + +PNDP+N GRALL+L G+IKLK + + AT DIVENPKK+K L+ Sbjct: 120 LNDLKQGARVGVPNDPTNGGRALLVLQAKGVIKLKADAGLKATPLDIVENPKKIKIVELD 179 Query: 178 AATLPRVLNQVDVALINTNYALEAKLNPVKDSLFIEDANSPYANLLVAREDNKDSPAIKK 237 AA LPR L+ D A+IN NYA A L+P KD++ +E + PYAN++ R +KD P + K Sbjct: 180 AAQLPRSLDDFDAAVINGNYAESAGLSPTKDAIAVEASTGPYANVIAVRIADKDKPWVAK 239 Query: 238 LAAALNSPDVKKFIEEKYQGAIVPAF 263 L A +SP+VKKF+ EKY+G+++ ++ Sbjct: 240 LVKAYHSPEVKKFVLEKYKGSVITSW 265 >gi|300314030|ref|YP_003778122.1| metal ion ABC transporter periplasmic component/surface antigen protein [Herbaspirillum seropedicae SmR1] Length = 276 Score = 198 bits (504), Expect = 3e-52, Method: Compositional matrix adjust. Identities = 101/248 (40%), Positives = 154/248 (62%), Gaps = 8/248 (3%) Query: 23 AADKLV-IAATPVPHAEILEHIKPALAKE--GIDLQIKVFTDYVQPAAQTNEKQVDGNFF 79 AAD+++ + T PHA+I E + ++ G ++I F DY+QP A + ++D N + Sbjct: 30 AADQVIRVGVTSGPHAQIFEQARRVFERDNPGYKVKIIEFNDYIQPNAALDAGELDANSY 89 Query: 80 LHQPYLDQFKKSHKNDIEVPVAKVHVEPFAAYSQKYKKIADIPNGATIAIPNDPSNSGRA 139 H+P+L+ K+ + K + P A YS+KY+K+ D+P GA I IPNDP+N R Sbjct: 90 QHRPFLNAQIKTRGYKLYAE-GKTMIGPMAIYSRKYRKLEDVPVGARIGIPNDPANESRV 148 Query: 140 LLLLDRNGLIKLKDN----TNISATQKDIVENPKKLKFRALEAATLPRVLNQVDVALINT 195 LLLL ++G IKL+ T +AT DI ENPKK KF ++AA LPR L+ +D + +N Sbjct: 149 LLLLQKHGFIKLRAGIDPLTGTNATPIDITENPKKWKFVEIDAAQLPRTLDDLDASAVNA 208 Query: 196 NYALEAKLNPVKDSLFIEDANSPYANLLVAREDNKDSPAIKKLAAALNSPDVKKFIEEKY 255 +YA +A LNP +DSL +E +SPYA L+ RE ++ P + KL A SP+VK++IE ++ Sbjct: 209 DYAAKAGLNPARDSLVVESGDSPYACLIAVREKDRAQPWLGKLVQAYQSPEVKRYIETEF 268 Query: 256 QGAIVPAF 263 +G I+ A+ Sbjct: 269 KGGILAAW 276 >gi|300311120|ref|YP_003775212.1| metal ion ABC transporter periplasmic component/surface antigen protein [Herbaspirillum seropedicae SmR1] Length = 274 Score = 183 bits (465), Expect = 1e-47, Method: Compositional matrix adjust. Identities = 103/248 (41%), Positives = 151/248 (60%), Gaps = 6/248 (2%) Query: 21 AHAADKLVI-AATPVPHAE-ILEHIKPALAKEGIDLQIKVFTDYVQPAAQTNEKQVDGNF 78 A DK I V AE I + +K A++G+++Q VF+DY+QP A +D N Sbjct: 28 GQAQDKQKIKVGISVGSAEAIFDVVKKVAARDGLEIQTVVFSDYLQPNAALAAGDLDANA 87 Query: 79 FLHQPYLDQFKKSHKNDIEVPVAKVHVEPFAAYSQKYKKIADIPNGATIAIPNDPSNSGR 138 F H+PYL+ + I VPV P YS+K++ + +P GA I I NDPSN R Sbjct: 88 FQHRPYLESQIAARGYRI-VPVGLTITAPLGIYSRKFRSVEQLPQGAAIGIQNDPSNGNR 146 Query: 139 ALLLLDRNGLIKLK---DNTNISATQKDIVENPKKLKFRALEAATLPRVLNQVDVALINT 195 ALLLL + GLI+LK ++AT D++ENP+KLK L+AA LPR L+ + A IN Sbjct: 147 ALLLLQKAGLIRLKPGVGENGVNATPLDVIENPRKLKLVELDAAQLPRSLDDLAAASINN 206 Query: 196 NYALEAKLNPVKDSLFIEDANSPYANLLVAREDNKDSPAIKKLAAALNSPDVKKFIEEKY 255 +YA +A L+ KD++ +ED YAN++ R ++KD P ++KL A +S +V+KFIE ++ Sbjct: 207 DYAFKAGLSLQKDTIAVEDPRGRYANIIATRAEDKDRPWVRKLVQAYHSEEVRKFIETEF 266 Query: 256 QGAIVPAF 263 +G++VPAF Sbjct: 267 KGSLVPAF 274 >gi|300311637|ref|YP_003775729.1| metal ion ABC transporter periplasmic component/surface antigen protein [Herbaspirillum seropedicae SmR1] Length = 268 Score = 120 bits (301), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 78/222 (35%), Positives = 113/222 (50%), Gaps = 8/222 (3%) Query: 21 AHAADKLVIAATPVPHAEILEHIKPALAKEGIDLQIKVFTDYVQPAAQTNEKQVDGNFFL 80 A AADKL I P + + + ++GID+Q+ FTD+ P + +D N+F Sbjct: 19 AQAADKLRIGVVPGAYGDSVAVAAKEAKRQGIDVQVVEFTDWTTPNVALDAGDLDLNYFQ 78 Query: 81 HQPYLDQFKKSHKNDIEVPVAKVHV-EPFAAYSQKYKKIADIPNGATIAIPNDPSNSGRA 139 HQP+LD KN ++ A + YS KYK + +P GA + I NDP N GRA Sbjct: 79 HQPFLD--NAIQKNGYKLASAGTGILSNVGLYSLKYKSLTALPQGAKVGIANDPVNQGRA 136 Query: 140 LLLLDRNGLIKLKDNTNISATQKDIVENPKKLKFRALEAATLPRVLNQVDVALINTNYAL 199 LLLL GLIKLK + DI++NP+KL F +E L R+ VD+A ++ + Sbjct: 137 LLLLQNVGLIKLKPKVGYLGSIDDIIDNPRKLSFVEVEGPQLVRITPDVDLAQGYPHFIV 196 Query: 200 EAK-LNPVKDSLF--IEDANSPYANLLVAREDNKDSPAIKKL 238 AK +P + IEDA +A V R + + P ++K Sbjct: 197 AAKAFDPSSGLAYSGIEDAR--FAIQFVTRANRVNDPVVQKF 236 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.133 0.372 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 781,652 Number of extensions: 31102 Number of successful extensions: 106 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 99 Number of HSP's successfully gapped: 5 Length of query: 263 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 180 Effective length of database: 1,226,865 Effective search space: 220835700 Effective search space used: 220835700 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 129 (54.3 bits)