BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0029.0092.p (547 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310882|ref|YP_003774974.1| 2-polyprenyl-6-methoxyphenol hy... 1112 0.0 gi|300309878|ref|YP_003773970.1| 2-polyprenyl-6-methoxyphenol hy... 590 e-170 gi|300313232|ref|YP_003777324.1| 3-(3-hydroxyphenyl) propionate ... 78 2e-15 >gi|300310882|ref|YP_003774974.1| 2-polyprenyl-6-methoxyphenol hydroxylase [Herbaspirillum seropedicae SmR1] Length = 547 Score = 1112 bits (2877), Expect = 0.0, Method: Compositional matrix adjust. Identities = 541/547 (98%), Positives = 541/547 (98%) Query: 1 MHSTYTYPRFAYRQSDEQREARPSRHPVVVIGAGPIGLTAALECAARGLPVVVLDDNDTV 60 MHSTYTYPRFAYRQSDEQREARPSRHPVVVIGAGPIGLTAALECAARGLPVVVLDDNDTV Sbjct: 1 MHSTYTYPRFAYRQSDEQREARPSRHPVVVIGAGPIGLTAALECAARGLPVVVLDDNDTV 60 Query: 61 SIGSRAVCYAKRPLEIWDRLGVAPRMVEKGVSWKLGKVFFEEREVYRFDLLPEHAHKMPA 120 SIGSRAVCYAKRPLEIWDRLGVAPRMVEKGVSWKLGKVFFEEREVYRFDLLPEHAHKMPA Sbjct: 61 SIGSRAVCYAKRPLEIWDRLGVAPRMVEKGVSWKLGKVFFEEREVYRFDLLPEHAHKMPA 120 Query: 121 MINLQQYYLEQYLVEACQAQPLIDLRWKHKMISLHQDDTHATLAVETPDGIFRMEADWVI 180 MINLQQYYLEQYLVEACQAQPLIDLRWKHKMISLHQDDTHATL VETPDGIFRMEADWVI Sbjct: 121 MINLQQYYLEQYLVEACQAQPLIDLRWKHKMISLHQDDTHATLDVETPDGIFRMEADWVI 180 Query: 181 ACDGANSDTRKMVGAEFSGQFFQDRFLIADVIMKADFPAERWFWFDPPFHRGQSVLLHKQ 240 ACDGANSDTRKMVGAEFSGQFFQDRFLIADVIMKADFPAERWFWFDPPFHRGQSVLLHKQ Sbjct: 181 ACDGANSDTRKMVGAEFSGQFFQDRFLIADVIMKADFPAERWFWFDPPFHRGQSVLLHKQ 240 Query: 241 CDNVWRIDFQLGWEADPEEEKKPEKVIPRIQAMLGKEVEFELEWVSVYQFACRRIDKMRY 300 CDNVWRIDFQLGWEADPEEEKKPEKVIPRIQAMLGKEVEFELEWVSVYQFACRRIDKMRY Sbjct: 241 CDNVWRIDFQLGWEADPEEEKKPEKVIPRIQAMLGKEVEFELEWVSVYQFACRRIDKMRY 300 Query: 301 QRVLFAGDAAHQVSPFGARGANTGVQDIDNLVWKLKLVIDGKAPLSFIDSYHEERAWAAD 360 QRVLFAGDAAHQVSPFGARGANTGVQDIDNLVWKLKLVIDGKAP SFIDSYHEERAWAAD Sbjct: 301 QRVLFAGDAAHQVSPFGARGANTGVQDIDNLVWKLKLVIDGKAPPSFIDSYHEERAWAAD 360 Query: 361 DNLRQSTRSTDFITPKSRASKALRDAVLGLAETEEFARTLVNSGRLSTPTPYVHSPLNTA 420 DNLRQSTRSTDFITPKSRASKALRDAVLGLAETEEFARTLVNSGRLSTPT YVHSPLNTA Sbjct: 361 DNLRQSTRSTDFITPKSRASKALRDAVLGLAETEEFARTLVNSGRLSTPTSYVHSPLNTA 420 Query: 421 DAALFGGRMAPGTVCADAPVLREGQSGWLLESLGAGFVVLFFGASAPQAVIEAAGISARV 480 DAALFGGRMAPGTVCADAPVLREGQ GWLLESLGAGFVVLFFGASAPQAVIEAAGISARV Sbjct: 421 DAALFGGRMAPGTVCADAPVLREGQPGWLLESLGAGFVVLFFGASAPQAVIEAAGISARV 480 Query: 481 VTVGAPGSGAELIDREGLAALRYAASEGCVYLVRPDQHVAARWNRFHSDDIVAALARCVG 540 VTVGAPGSGAELIDREGLAALRYAASEGCVYLVRPDQHVAARWNRFHSDDI AALARCVG Sbjct: 481 VTVGAPGSGAELIDREGLAALRYAASEGCVYLVRPDQHVAARWNRFHSDDIAAALARCVG 540 Query: 541 QEMALAA 547 QEM LAA Sbjct: 541 QEMPLAA 547 >gi|300309878|ref|YP_003773970.1| 2-polyprenyl-6-methoxyphenol hydroxylase [Herbaspirillum seropedicae SmR1] Length = 589 Score = 590 bits (1521), Expect = e-170, Method: Compositional matrix adjust. Identities = 302/547 (55%), Positives = 372/547 (68%), Gaps = 28/547 (5%) Query: 5 YTYPRFAYRQSDEQRE-ARPSRHPVVVIGAGPIGLTAALECAARGLPVVVLDDNDTVSIG 63 Y RFA+ EQ+ HPVV+IGAGP+GL A++ A +G+ VV++DD+DT+S G Sbjct: 9 YQQQRFAWTPCAEQQSPGHAPVHPVVIIGAGPVGLACAIDLARQGIAVVLVDDDDTLSTG 68 Query: 64 SRAVCYAKRPLEIWDRLGVAPRMVEKGVSWKLGKVFFEEREVYRFDLLPEHAHKMPAMIN 123 SRA+C+AKR L+I+DR+G RMV+KGV W GKVF ++ VY FDLLPE H PA IN Sbjct: 69 SRAICFAKRTLDIFDRIGCGQRMVDKGVGWNRGKVFLRDQAVYEFDLLPESGHARPAFIN 128 Query: 124 LQQYYLEQYLVEACQAQPLIDLRWKHKMISLHQDDTHATLAVETPDGIFRMEADWVIACD 183 LQQYY+E YL+E Q P I++RWK+K+ ++ + H L V+TP G + ++A +V+A D Sbjct: 129 LQQYYVEGYLLEQAQQWPHIEIRWKNKVSAIAHHEDHEALTVDTPQGSYTLKARYVVAAD 188 Query: 184 GANSDTRKMVGAEFSGQFFQDRFLIADVIMKADFPAERWFWFDPPFHRGQSVLLHKQCDN 243 G+ S RKM+G E G F+DRFLIADV M A FPAERWFWFDPPFH QSVLLH+Q D+ Sbjct: 189 GSRSPLRKMMGLESQGHTFRDRFLIADVKMDAPFPAERWFWFDPPFHPQQSVLLHRQPDD 248 Query: 244 VWRIDFQLGWEADPEEEKKPEKVIPRIQAMLGKEVEFELEWVSVYQFACRRIDKMRYQRV 303 VWRIDFQLGW+ADP EK PE+VIPR++A+LG+EV F LEWVSVY F+C R+++ R+ RV Sbjct: 249 VWRIDFQLGWDADPVLEKTPERVIPRVRALLGEEVSFSLEWVSVYTFSCVRMERFRHGRV 308 Query: 304 LFAGDAAHQVSPFGARGANTGVQDIDNLVWKLKLVIDGKAPLSFIDSYHEERAWAADDNL 363 LF GDAAH VSPFGARGAN+G+QD +NL WKL V+ GKAP S +DSY ER +AAD+NL Sbjct: 309 LFVGDAAHCVSPFGARGANSGIQDAENLAWKLAHVLQGKAPDSLLDSYASEREYAADENL 368 Query: 364 RQSTRSTDFITPKSRASKALRDAVLGLAETEEFARTLVNSGRLSTPTPYVHSPLNTAD-- 421 STRSTDFITPKS S+A RDAVLGLA FAR LVNSGRLS P SPLNT D Sbjct: 369 LNSTRSTDFITPKSAVSRAFRDAVLGLAREHAFARRLVNSGRLSLPATLDQSPLNTPDQA 428 Query: 422 ----AALFGGRMAPGTVCADAPVLREGQSGWLLESLGAGFVVLFFGASA--PQAVIEAAG 475 A F G M PG C DAP+L+ GQ WLL LG FV++ F A A P A +A Sbjct: 429 AAPEAVAFTGPMRPGAPCCDAPLLQAGQPAWLLPQLGDRFVLMIFAAPAQLPAATRQALA 488 Query: 476 ------------ISARVVT-------VGAPGSGAELIDREGLAALRYAASEGCVYLVRPD 516 ISA+ T G ++ D + LAA RY A G YL+RPD Sbjct: 489 ALAQPDALRLLFISAQAATQEDERVLQTLAGQAIQVQDIDTLAAQRYDAHPGSCYLIRPD 548 Query: 517 QHVAARW 523 QHV+ARW Sbjct: 549 QHVSARW 555 >gi|300313232|ref|YP_003777324.1| 3-(3-hydroxyphenyl) propionate hydroxylase [Herbaspirillum seropedicae SmR1] Length = 544 Score = 77.8 bits (190), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 86/350 (24%), Positives = 141/350 (40%), Gaps = 46/350 (13%) Query: 28 VVVIGAGPIGLTAALECAARGLPVVVLDDNDTVSIGSRAVCYAKRPLEIWDRLGVAPRMV 87 V+++GAGP+G+T G+ ++++ N V RAV L + G+A +V Sbjct: 19 VLIVGAGPVGVTLGNLLGTYGISTLLIERNLDVLDYPRAVGIDDEALRTFQSAGMADAIV 78 Query: 88 EKGVSWKLGKVFFEEREVYRFDLLPEHAHKMPAMINLQQY---YLEQYLVEACQAQPL-- 142 + +++ +R + A P Y Q L EA L Sbjct: 79 RDVIQNVPMRMYTADRRCF--------AEIQPTTREFGWYRRNLFSQPLGEATLRLGLRR 130 Query: 143 ---IDLRWKHKMISLHQDDTHATLAVETPDGI-FRMEADWVIACDGANSDTR-KMVGAEF 197 + LR +++ L QD+ ++ G + A +V+ CDG S R K++ + Sbjct: 131 FEHVSLRSGVELLDLVQDEAGVQASIIDEHGAQATLRAQYVVGCDGGRSTVREKILKLPY 190 Query: 198 SGQFFQDRFLIADVIMKADFPAERWFWFDPPFHRGQSVLLHKQCD------------NVW 245 G+ ++ V+++ D D P+ LH C+ V Sbjct: 191 EGKTHPAKW----VVIECDGDP-----LDAPY-----TALH--CEPQRPYVCLALPYGVR 234 Query: 246 RIDFQLGWEADPEEEKKPEKVIPRIQAMLGKEVEFELEWVSVYQFACRRIDKMRYQRVLF 305 R +F L D E+ PEKV ++ + + VY R + R+ Sbjct: 235 RWEFMLFPGEDGEQMLAPEKVRELLREHVRDVDALNIIRARVYTHNSRVAGSLVCGRICL 294 Query: 306 AGDAAHQVSPFGARGANTGVQDIDNLVWKLKLVIDGKAPLSFIDSYHEER 355 AGDAAH P+ +G N G++D NL WKL V+ G+ + SYHEER Sbjct: 295 AGDAAHITPPWIGQGLNAGLRDAFNLAWKLAWVLQGRMKPELLASYHEER 344 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.136 0.422 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,858,449 Number of extensions: 81803 Number of successful extensions: 316 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 314 Number of HSP's successfully gapped: 3 Length of query: 547 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 457 Effective length of database: 1,193,720 Effective search space: 545530040 Effective search space used: 545530040 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)