BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0029.0088.p (660 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310875|ref|YP_003774967.1| acetyl-coenzyme A synthetase [H... 1333 0.0 gi|300312417|ref|YP_003776509.1| propionyl-CoA ligase [Herbaspir... 410 e-115 gi|300309365|ref|YP_003773457.1| acetyl-coenzyme A synthetase [H... 178 1e-45 gi|300310257|ref|YP_003774349.1| acetyl-coenzyme A synthetase [H... 157 3e-39 gi|300313157|ref|YP_003777249.1| long-chain-fatty-acid-CoA ligas... 118 1e-27 >gi|300310875|ref|YP_003774967.1| acetyl-coenzyme A synthetase [Herbaspirillum seropedicae SmR1] Length = 660 Score = 1333 bits (3449), Expect = 0.0, Method: Compositional matrix adjust. Identities = 645/660 (97%), Positives = 650/660 (98%) Query: 1 VADIETFKQENRVFAPPAELVKHAAISGMDAYHALNAEFAKDYEGTWARLAKDNLKWHKP 60 +ADIETFKQENRVFAPPAELVKHAAISGMDAYHALNAEFAKDYEGTWARLAKDNLKWHKP Sbjct: 1 MADIETFKQENRVFAPPAELVKHAAISGMDAYHALNAEFAKDYEGTWARLAKDNLKWHKP 60 Query: 61 FTQTLDESNAPFYKWFADGKVNVSYNCLDVNLENGNADKTAVIFESDDGKVTRVSYRELH 120 FTQTLDESNAPFYKWFADG+VNVSYNCLDVNLENGNADKTAVIFESDDGKVTRVSYRELH Sbjct: 61 FTQTLDESNAPFYKWFADGQVNVSYNCLDVNLENGNADKTAVIFESDDGKVTRVSYRELH 120 Query: 121 QKVCQFANGLKSLGIKKGDRVVIYMPMSVEGVAAMQACARIGATHSVVFGGFSAKSLHER 180 QKVCQFANGLKSLGIKKGDRVVIYMPMSVEGVAAMQACARIGATHSVVFGGFSAKSLHER Sbjct: 121 QKVCQFANGLKSLGIKKGDRVVIYMPMSVEGVAAMQACARIGATHSVVFGGFSAKSLHER 180 Query: 181 VIDAGAVAVLTADYQVRGGKQLPLKSIVDEALDMGGCDTLKHVVVYKRAGAEINWVEGRD 240 VIDAGAVAVLTADYQVRGGKQLPLKSIVDEALDMGGCDTLKHVVVYKRAGAEINWVEGRD Sbjct: 181 VIDAGAVAVLTADYQVRGGKQLPLKSIVDEALDMGGCDTLKHVVVYKRAGAEINWVEGRD 240 Query: 241 LWLSDVVANQPDTCEPEWVDAERTVCVRFFLFCFGKPKGVQHASAGYLLWAVLTMKWTFD 300 LWLSDVVANQ DTCEPEWVDAE + + + GKPKGVQHASAGYLLWAVLTMKWTFD Sbjct: 241 LWLSDVVANQSDTCEPEWVDAEHPLFILYTSGSTGKPKGVQHASAGYLLWAVLTMKWTFD 300 Query: 301 IKPDDVYWCTADIGWITGHTYIAYGPLAVGATQIVFEGVPTYPNAGRFWDMVARHKATIF 360 IKPDDVYWCTADIGWITGHTYIAYGPLAVGATQIVFEGVPTYPNAGRFWDMVARHKATIF Sbjct: 301 IKPDDVYWCTADIGWITGHTYIAYGPLAVGATQIVFEGVPTYPNAGRFWDMVARHKATIF 360 Query: 361 YTAPTAIRSLIKAADADEKVHPKQYDLSSLRILGSVGEPINPEAWMWYYKNVGHENCPIV 420 YTAPTAIRSLIKAADADEKVHPKQYDLSSLRILGSVGEPINPEAWMWYYKNVGHENCPIV Sbjct: 361 YTAPTAIRSLIKAADADEKVHPKQYDLSSLRILGSVGEPINPEAWMWYYKNVGHENCPIV 420 Query: 421 DTFWQTETGGHMISPLPGATPQVPGSCTLPLPGITAAIVDETGNDIPNGNGGILVVKRPW 480 DTFWQTETGGHMISPLPGATPQVPGSCTLPLPGITAAIVDETGNDIPNGNGGILVVKRPW Sbjct: 421 DTFWQTETGGHMISPLPGATPQVPGSCTLPLPGITAAIVDETGNDIPNGNGGILVVKRPW 480 Query: 481 PSMIRTIWNDPERFKKSYFPEELGGKIYLAGDGAVRNKETGYFTITGRIDDVLNVSGHRM 540 PSMIRTIWNDPERFKKSYFPEELGGKIYLAGDGAVRNKETGYFTITGRIDDVLNVSGHRM Sbjct: 481 PSMIRTIWNDPERFKKSYFPEELGGKIYLAGDGAVRNKETGYFTITGRIDDVLNVSGHRM 540 Query: 541 GTMEIESALVANSIVAEAAVVGKPDETTGESICAFVVLKRPRPTGDEAKQIAKELRDWVA 600 GTMEIESALVANSIVAEAAVVGKPDETTGESICAFVVLKRPRPTGDEAKQIAKELRDWVA Sbjct: 541 GTMEIESALVANSIVAEAAVVGKPDETTGESICAFVVLKRPRPTGDEAKQIAKELRDWVA 600 Query: 601 KEIGPIAKPKEIRFGDNLPKTRSGKIMRRLLRVLAKGEAITQDVSTLENPAILDQLKEAQ 660 KEIGPIAKPKEIRFGDNLPKTRSGKIMRRLLRVLAKGEAITQDVSTLENPAILDQLKEAQ Sbjct: 601 KEIGPIAKPKEIRFGDNLPKTRSGKIMRRLLRVLAKGEAITQDVSTLENPAILDQLKEAQ 660 >gi|300312417|ref|YP_003776509.1| propionyl-CoA ligase [Herbaspirillum seropedicae SmR1] Length = 628 Score = 410 bits (1055), Expect = e-115, Method: Compositional matrix adjust. Identities = 238/634 (37%), Positives = 368/634 (58%), Gaps = 21/634 (3%) Query: 32 YHALNAEFAKDYEGTWARLAKDNLKWHKPFTQTLDESNAPFYKWFADGKVNVSYNCLDVN 91 Y + + E WA+ A+ + W +PF++TLD S PF +WF DG+ N+ +N +D + Sbjct: 3 YEQFYRQSVEQPEAFWAQEAQ-RIHWQQPFSRTLDYSRPPFARWFIDGQTNLCHNAVDRH 61 Query: 92 LENGNADKTAVIFESDDGKVTRV-SYRELHQKVCQFANGLKSLGIKKGDRVVIYMPMSVE 150 L A + A+I S + RV +Y EL Q+V A +++LG+++GDRV+IYMPM E Sbjct: 62 LPE-RAQQAALIAISTETNSERVYTYAELQQEVMAMAASMQALGVQRGDRVLIYMPMIAE 120 Query: 151 GVAAMQACARIGATHSVVFGGFSAKSLHERVIDAGAVAVLTADYQVRGGKQLPLKSIVDE 210 V AM ACARIGA HSVVFGGF++ SL R+ DA +++AD RGGK +P K ++DE Sbjct: 121 AVFAMLACARIGAVHSVVFGGFASNSLASRIDDAQPRLIVSADAGSRGGKVIPYKGLLDE 180 Query: 211 ALDMGGCDTLKHVVVYKRAGAEINWVEGRDLWLSDVVANQPDTCEP-EWVDAERTVCVRF 269 A+ M +HV++ R A + RD+ + + P W+++ + + + Sbjct: 181 AIAMAQ-HRPQHVLLVDRGLAPMARTAQRDVDYAPLRTQHLGQQVPVTWLESNASSYILY 239 Query: 270 FLFCFGKPKGVQHASAGYLLWAVLTMKWTFDIKPDDVYWCTADIGWITGHTYIAYGPLAV 329 GKPKGVQ GY + +M+ F +P + Y+CT+DIGW+ GH+YI YGPL Sbjct: 240 TSGTTGKPKGVQRDVGGYAVALASSMQHIFCGQPGETYFCTSDIGWVVGHSYIVYGPLIA 299 Query: 330 GATQIVFEGVPTYPNAGRFWDMVARHKATIFYTAPTAIRSLIKAADADEKVHPKQYDLSS 389 G +++EG+P P+AG +W +V ++K T ++APTAIR L K ++YD SS Sbjct: 300 GMATVLYEGLPIRPDAGIWWSIVEKYKVTRMFSAPTAIRVLKKQPPECM----ERYDTSS 355 Query: 390 LRILGSVGEPINPEAWMWYYKNVGHENCPIVDTFWQTETGGHMISPLPGA--TPQVPGSC 447 L+ L GEP++ W G P++D +WQTE+G +IS G P GS Sbjct: 356 LKALYLAGEPLDETTSSWI---SGALKVPVIDNYWQTESGWPIISIAKGIDDKPTRLGSP 412 Query: 448 TLPLPGITAAIVDE-TGNDIPNGNGGILVVKRPW-PSMIRTIWNDPERFKKSYFPEELGG 505 +P+PG AI+DE TG G++ ++ P P ++T++ D ERF +Y+ L Sbjct: 413 GVPMPGYRLAILDEATGEPCGPDQKGVVAIEGPLPPGCMQTVYGDDERFVNTYW-RSLPR 471 Query: 506 KIYLAGDGAVRNKETGYFTITGRIDDVLNVSGHRMGTMEIESALVANSIVAEAAVVGKPD 565 ++Y D +R+K+ GY+ I GR DDV+NV+GHR+GT EIE ++ ++ V+E AVVG D Sbjct: 472 EVYSTFDWGIRDKD-GYYFILGRTDDVINVAGHRLGTREIEESISSHPNVSEVAVVGVED 530 Query: 566 ETTGESICAFVVLKRPR--PTGDEAKQIAKELRDWVAKEIGPIAKPKEIRFGDNLPKTRS 623 + G+ AF + K+ T ++ K + E+ V K+IG + +P + F LPKTRS Sbjct: 531 KLKGQVAFAFAIPKQAYTIATPEQRKALEAEIMAVVDKQIGAVGRPARVFFVSGLPKTRS 590 Query: 624 GKIMRRLLRVLAKGEAITQDVSTLENPAILDQLK 657 GK++RR ++ + +G D+ T+E+PA L+Q++ Sbjct: 591 GKLLRRAIQSICEGRD-PGDLPTIEDPAALEQVR 623 >gi|300309365|ref|YP_003773457.1| acetyl-coenzyme A synthetase [Herbaspirillum seropedicae SmR1] Length = 549 Score = 178 bits (452), Expect = 1e-45, Method: Compositional matrix adjust. Identities = 167/566 (29%), Positives = 259/566 (45%), Gaps = 42/566 (7%) Query: 69 NAPFYKWFADGKVNVSYNCLDVNLENGNADKTAVIFESDDGKVTRVSYRELHQKVCQFAN 128 N Y+W N++ D +G+ +TA+I E DG VT SY +L +FAN Sbjct: 20 NPSPYEWQIPTHFNIAQAACD-RWADGSG-RTAIICEDADGSVTSYSYDQLKILSDRFAN 77 Query: 129 GLKSLGIKKGDRVVIYMPMSVEGVAAMQACARIGATHSVVFGGFSAKSLHERVIDAGAVA 188 L++ G+ +GDR+ IY+ +E V A ++GA +F F ++ R+ ++GAVA Sbjct: 78 ALRAAGVGRGDRIGIYLSQRIETVITHLAAYKLGAITVPLFYLFGPDAIAYRLDNSGAVA 137 Query: 189 VLTADYQVRGGKQLPLKSIVDEALDMGGCDTLKHVVVYKRAGAEINWVEGRDLWLSDVVA 248 ++T S +++A G L+ +V+ +++ + D W D + Sbjct: 138 LVTD------------ASGMEKATLAGELPALR--LVFCVESSDLVLPQTTDFW--DRLH 181 Query: 249 NQPDTCEPEWVDAERTVCVRFFLFCFGKPKGVQHASAGYLLWAVLTMKWTFDIKPD--DV 306 P +P A+ + + GK KG HA LL + ++ + D P D Sbjct: 182 AAPAELDPVLTLADDPAMIIYTSGTTGKAKGALHAHR-VLLGHLPGVEVSHDSFPQEGDR 240 Query: 307 YWCTADIGWITGHTYIAYGPLAVGATQIVFEGVPTYPNAGRFWDMVARHKATIFYTAPTA 366 +W AD WI G + L G +V + + +A + ++ARH+ + PTA Sbjct: 241 FWTPADWAWIGGLLDVLLPSLYHGVA-VVARRLEKF-DAAEVFGLLARHQIRNVFFPPTA 298 Query: 367 IRSLIKAADADEKVHPKQYDLSSLRILGSVGEPINPEAWMWYYKNVGHENCPIVDTFWQT 426 ++ L AA Q D SLR + S GE + + W + +G I + + QT Sbjct: 299 LKMLRGAATVR-----AQADF-SLRSVASGGETLGDDLIAWGREALG---VTINEFYGQT 349 Query: 427 ETGGHMISPLPGATPQVPGSCTLPLPGITAAIVDETGNDIPNGNGGILVVKRPWPSMIRT 486 E ++S P V GS +PG IVDE G +P+G G + ++ P P M Sbjct: 350 EC-NLVVSSSSHCYPSVSGSMGRAVPGHVVQIVDEQGQVLPHGTVGNIAIRAPDPVMFLR 408 Query: 487 IWNDPERFKKSYFPEELGGKIYLAGDGAVRNKETGYFTITGRIDDVLNVSGHRMGTMEIE 546 W + E + E+ G L GD + E GY GR DDV+ +G+R+G IE Sbjct: 409 YWRNEEATR-----EKFAGDFLLTGDLGSMD-EQGYIRYLGRNDDVITSAGYRIGPAAIE 462 Query: 547 SALVANSIVAEAAVVGKPDETTGESICAFVVLKRPRPTGDEAKQIAKELRDWVAKEIGPI 606 L+ + V AAVVG D E + AFVVLK T DEA + EL+ V ++ Sbjct: 463 ECLMRHPAVRIAAVVGVKDALRTEVVKAFVVLKDG-VTPDEA--LKAELQQHVRAQLAAH 519 Query: 607 AKPKEIRFGDNLPKTRSGKIMRRLLR 632 P+ I F LP T +GKIMR+ L+ Sbjct: 520 EYPRLISFVAALPTTATGKIMRKTLK 545 >gi|300310257|ref|YP_003774349.1| acetyl-coenzyme A synthetase [Herbaspirillum seropedicae SmR1] Length = 556 Score = 157 bits (397), Expect = 3e-39, Method: Compositional matrix adjust. Identities = 147/555 (26%), Positives = 235/555 (42%), Gaps = 49/555 (8%) Query: 81 VNVSYNCLDVNLENGNADKTAVIFESDDGKVTRVSYRELHQKVCQFANGLKSLGIKKGDR 140 +N C D +++ D A+ F S G+ + S+ ++ + AN L+ GI+ G Sbjct: 37 INACVECCDRHVKG---DNIALEFVSLHGEHQQYSFAQVRSMAARVANLLRQQGIQPGQI 93 Query: 141 VVIYMPMSVEGVAAMQACARIGATHSVVFGGFSAKSLHERVIDAGAVAVLTADYQVRGGK 200 V +P + E +A + R GA + +F F K++ R+ +GA ++T Sbjct: 94 VAGMLPRTPELLATVLGTLRAGAVYQPLFTAFGPKAIEHRLALSGASLIVT--------- 144 Query: 201 QLPLKSIVDEALDMGGCDTLKHVVVYKRAGAEINWVEGRDLWLSDVVANQPDTCEPEWVD 260 + + +DE + T++ RAG D+ V Q D P Sbjct: 145 NVANRDKLDEIANCPQVCTVRESNDPLRAG---------DIDFRAAVDAQSDDFAPVLRS 195 Query: 261 AERTVCVRFFLFCFGKPKGVQHASAGYLLWAVLTMKWTFDIKPDDVYWCTADIGWITGHT 320 + + G KGV + ++V M+ + P D +W AD GW G Sbjct: 196 GADLMLMMSTSGTTGAAKGVPVPLSALQAFSVY-MREAVGLLPQDKFWNMADPGWAYGLY 254 Query: 321 YIAYGPLAVGATQIVFEGVPTYPNAGRFWDMVARHKATIFYTAPTAIRSLIKAADADEKV 380 Y GPLA+G E T ++ + R T APTA R ++ A + Sbjct: 255 YAIIGPLAIGHGITFNEAAFTVSGT---YETIRRLGITSLAGAPTAYRMMMAAGEEAAAS 311 Query: 381 HPKQYDLSSLRILGSVGEPINPEAWMWYYKNVGHENCPIVDTFWQTETGGHMISPLPGAT 440 + LR + S GEP+N E W+ + + PI D + QTE G + + Sbjct: 312 VKGR-----LRAVSSAGEPLNAEVVRWFASAL---DVPIHDHYGQTELGMVVNNHHALRH 363 Query: 441 PQVPGSCTLPLPGITAAIVDETGNDIPNGNGGILVVKRPWPSMIRTIWNDPERFKKSYFP 500 VPGS +PG ++DE ++ G L + I P + Y+ Sbjct: 364 EVVPGSAGFAMPGYRVVVLDENHQELGANQPGELAID---------IARSPLYWFSGYWK 414 Query: 501 EE---LGGKIYLAGDGAVRNKETGYFTITGRIDDVLNVSGHRMGTMEIESALVANSIVAE 557 ++ + Y GD V + G + GR DDV+ +G+R+G ++ESAL+ + VA+ Sbjct: 415 QDTPAIAHGYYSTGDN-VELEPNGSISFIGRSDDVITSAGYRIGPFDVESALLEHPAVAD 473 Query: 558 AAVVGKPDETTGESICAFVVLKRPRPTGDEAKQIAKELRDWVAKEIGPIAKPKEIRFGDN 617 AV+G PD E + AFVVL D ++ +L V + + A P+ I F D Sbjct: 474 VAVIGLPDPERTEIVKAFVVLSDQFKASDALRE---QLAQHVKRRLSAHAYPRAIEFLDA 530 Query: 618 LPKTRSGKIMRRLLR 632 LPKT SGK+ R +LR Sbjct: 531 LPKTPSGKLQRFVLR 545 >gi|300313157|ref|YP_003777249.1| long-chain-fatty-acid-CoA ligase [Herbaspirillum seropedicae SmR1] Length = 566 Score = 118 bits (296), Expect = 1e-27, Method: Compositional matrix adjust. Identities = 148/553 (26%), Positives = 224/553 (40%), Gaps = 81/553 (14%) Query: 114 VSYRELHQKVCQFANGLKSLGIKKGDRVVIYMPMSVEGVAAMQACARIGATHSVVFGGFS 173 +SYREL L+ LG++KG RV I MP ++ A+ A R G T V ++ Sbjct: 49 LSYRELDSLSRALGGWLQGLGLQKGARVAIMMPNVLQYPIAIAAILRAGYTVVNVNPLYT 108 Query: 174 AKSLHERVIDAGAVAVLTADYQVRGGKQLPLKSIVDEALDMGGCDTLKHVVVYKRAGAEI 233 A+ L ++ D+GA A+ + L+ +V +KH+VV G + Sbjct: 109 ARELEHQLKDSGAEAIFVLE-----NFACTLQKVVAHT-------NIKHIVV-ASMGELL 155 Query: 234 NWVEGRDLWLSDVVANQPDTCEPEWVDAERTVCVRFFLFCFGK---PKGVQHASAGYLLW 290 ++G L ++V P W + GK P + H +L + Sbjct: 156 GALKGT---LVNLVVRHVKKLVPAWSLPNAITFSQALSQARGKTLQPVTLTHDDIAFLQY 212 Query: 291 ----------AVLTMK-----------WTFDI----KPDD--VYWCTADIGWITGHTYIA 323 AVLT + W KP D V+ C + I T Sbjct: 213 TGGTTGVAKGAVLTHRNLVANVLQAEEWLTPALKAGKPIDQLVFVCALPLYHIFALTVCN 272 Query: 324 YGPLAVGATQIVFEGVPTYPNAGRFWDMVARHKATIFYTAPTAIRSLIKAADADEKVHPK 383 GA ++ +P + F +A++K F T +L+ AD Sbjct: 273 MLGTREGALNLL---IPNPRDIPGFVKELAKYKVNTFPAVNTLYNALLNNADF------A 323 Query: 384 QYDLSSLRILGSVGEPINPEAWMWYYKNVGHENCPIVDTFWQTETGGHMISPLPGATP-- 441 + D S R G + + K G CPI++ + +ET SP+ A P Sbjct: 324 RLDFSGYRCCVGGGMAVQKSVADKWKKLTG---CPIIEGYGLSET-----SPIASANPCT 375 Query: 442 --QVPGSCTLPLPGITAAIVDETGNDIPNGNGGILVVKRPWPSMIRTIWNDPERFKKSYF 499 + G+ LPLP AI+D+ G +P G G + ++ P ++ W P+ K Sbjct: 376 IDEYTGTIGLPLPSTEFAILDDDGQPVPLGQPGEIAIR--GPQVMPGYWQRPDETAKVMT 433 Query: 500 PEELGGKIYLAGDGAVRNKETGYFTITGRIDDVLNVSGHRMGTMEIESALVANSIVAEAA 559 P+ + GD V + E GY I R D++ VSG + E+E + + V E A Sbjct: 434 PD----GFFKTGDIGVMD-ERGYTKIVDRKKDMILVSGFNVYPNEVEGVVAQHPGVLECA 488 Query: 560 VVGKPDETTGESICAFVVLKRPRPTGDEAKQIAKELRDWVAKEIGPIAKPKEIRFGDNLP 619 VG PDE TGE++ FVV K + A +L D+ ++ KPK I F D LP Sbjct: 489 CVGVPDEHTGEAVKLFVVRK-------DKTLSAAQLMDYCKEQFTGYKKPKYIEFRDELP 541 Query: 620 KTRSGKIMRRLLR 632 KT GKI+RR LR Sbjct: 542 KTNVGKILRRELR 554 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.423 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,269,321 Number of extensions: 102574 Number of successful extensions: 278 Number of sequences better than 1.0e-08: 10 Number of HSP's gapped: 258 Number of HSP's successfully gapped: 10 Length of query: 660 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 569 Effective length of database: 1,188,985 Effective search space: 676532465 Effective search space used: 676532465 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)