BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0029.0066.p (515 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310856|ref|YP_003774948.1| major facilitator superfamily p... 882 0.0 gi|300311040|ref|YP_003775132.1| major facilitator superfamily p... 515 e-147 gi|300313188|ref|YP_003777280.1| major facilitator superfamily p... 342 4e-95 gi|300312683|ref|YP_003776775.1| major facilitator superfamily p... 253 3e-68 gi|300312017|ref|YP_003776109.1| multidrug resistance B (translo... 135 8e-33 >gi|300310856|ref|YP_003774948.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 446 Score = 882 bits (2279), Expect = 0.0, Method: Compositional matrix adjust. Identities = 446/446 (100%), Positives = 446/446 (100%) Query: 70 MDSTVISTSLPALALDMNVDPISLKLALTSYLVSLAVFIPISGWMADRFGSLTIFRSAIC 129 MDSTVISTSLPALALDMNVDPISLKLALTSYLVSLAVFIPISGWMADRFGSLTIFRSAIC Sbjct: 1 MDSTVISTSLPALALDMNVDPISLKLALTSYLVSLAVFIPISGWMADRFGSLTIFRSAIC 60 Query: 130 VFMLGSILCGVSSTLPQFVAARFLQGIGGAMMVPVGRLVILRSVEKVNLVKALSYLTIPA 189 VFMLGSILCGVSSTLPQFVAARFLQGIGGAMMVPVGRLVILRSVEKVNLVKALSYLTIPA Sbjct: 61 VFMLGSILCGVSSTLPQFVAARFLQGIGGAMMVPVGRLVILRSVEKVNLVKALSYLTIPA 120 Query: 190 LFGPVLGPPLGGFITTYFHWRWIFFINIPISILGLYLATRFIPDLKAEQVAPLDLKGFLL 249 LFGPVLGPPLGGFITTYFHWRWIFFINIPISILGLYLATRFIPDLKAEQVAPLDLKGFLL Sbjct: 121 LFGPVLGPPLGGFITTYFHWRWIFFINIPISILGLYLATRFIPDLKAEQVAPLDLKGFLL 180 Query: 250 SALGCSLLMMGLATAGRHLVSGEVALTCVLAGLVFLAAYVWHARRASAPLIDLALLKIPT 309 SALGCSLLMMGLATAGRHLVSGEVALTCVLAGLVFLAAYVWHARRASAPLIDLALLKIPT Sbjct: 181 SALGCSLLMMGLATAGRHLVSGEVALTCVLAGLVFLAAYVWHARRASAPLIDLALLKIPT 240 Query: 310 LRAGVFGGSFYRVGIGAIPFLLPLMLQLGFGYTPFHSGMLTCASAAGAMFMKTIAARFLR 369 LRAGVFGGSFYRVGIGAIPFLLPLMLQLGFGYTPFHSGMLTCASAAGAMFMKTIAARFLR Sbjct: 241 LRAGVFGGSFYRVGIGAIPFLLPLMLQLGFGYTPFHSGMLTCASAAGAMFMKTIAARFLR 300 Query: 370 RYGFRRVLLVNGALGVLGLWAIATFTPQTPYLLMVVILLIGGCFRSLQFTSLNAISYADV 429 RYGFRRVLLVNGALGVLGLWAIATFTPQTPYLLMVVILLIGGCFRSLQFTSLNAISYADV Sbjct: 301 RYGFRRVLLVNGALGVLGLWAIATFTPQTPYLLMVVILLIGGCFRSLQFTSLNAISYADV 360 Query: 430 DKLRLSQATSMASVAQQLSIGMGVVLAGFALQMSNEIQGHATLVAQDFWPAFLVVGAVAA 489 DKLRLSQATSMASVAQQLSIGMGVVLAGFALQMSNEIQGHATLVAQDFWPAFLVVGAVAA Sbjct: 361 DKLRLSQATSMASVAQQLSIGMGVVLAGFALQMSNEIQGHATLVAQDFWPAFLVVGAVAA 420 Query: 490 CALLMLRQLPHDAGAEMAGRDDNRPA 515 CALLMLRQLPHDAGAEMAGRDDNRPA Sbjct: 421 CALLMLRQLPHDAGAEMAGRDDNRPA 446 >gi|300311040|ref|YP_003775132.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 441 Score = 515 bits (1326), Expect = e-147, Method: Compositional matrix adjust. Identities = 252/438 (57%), Positives = 331/438 (75%) Query: 70 MDSTVISTSLPALALDMNVDPISLKLALTSYLVSLAVFIPISGWMADRFGSLTIFRSAIC 129 MD+TV++TSLPALA D++ DPI+LKLA+T+Y+ +L VFIPISGWMAD+ G+ +F +A+ Sbjct: 1 MDATVLATSLPALARDLHQDPITLKLAMTAYVAALGVFIPISGWMADKLGARKVFSAAMI 60 Query: 130 VFMLGSILCGVSSTLPQFVAARFLQGIGGAMMVPVGRLVILRSVEKVNLVKALSYLTIPA 189 VFM GSILC VS +L FVAARFLQGIGGAMMVPVGR++I RSV+K LVKA+SYLT+PA Sbjct: 61 VFMTGSILCAVSGSLVTFVAARFLQGIGGAMMVPVGRVIIARSVDKSELVKAVSYLTLPA 120 Query: 190 LFGPVLGPPLGGFITTYFHWRWIFFINIPISILGLYLATRFIPDLKAEQVAPLDLKGFLL 249 L GPV+GP LGGFITTYFHWRW+F INIP LGLYLA RFI + K + APLD+KGF+L Sbjct: 121 LLGPVVGPLLGGFITTYFHWRWVFLINIPFCFLGLYLARRFIQNFKEDNPAPLDVKGFVL 180 Query: 250 SALGCSLLMMGLATAGRHLVSGEVALTCVLAGLVFLAAYVWHARRASAPLIDLALLKIPT 309 +++G +++M+GL+ G HLV G + G + L Y H+ + PL+D LL+IPT Sbjct: 181 TSVGAAMIMLGLSLFGSHLVDGATPVAMCSIGAIALVMYYRHSTQIEFPLLDFRLLRIPT 240 Query: 310 LRAGVFGGSFYRVGIGAIPFLLPLMLQLGFGYTPFHSGMLTCASAAGAMFMKTIAARFLR 369 L A V GGS +R+G+GA+PFLLPL LQ G G T F +G +TCASA G++F++ +A R LR Sbjct: 241 LHASVVGGSLFRIGLGAVPFLLPLALQEGLGMTAFQAGSITCASAFGSLFIRLLAGRVLR 300 Query: 370 RYGFRRVLLVNGALGVLGLWAIATFTPQTPYLLMVVILLIGGCFRSLQFTSLNAISYADV 429 RYGFR VL+VN + L A F+ TPYLL+V I+L+GG F +LQFT LN+I+YAD+ Sbjct: 301 RYGFRTVLMVNAVFAGIALAAYGIFSIHTPYLLIVSIVLMGGVFPALQFTCLNSIAYADI 360 Query: 430 DKLRLSQATSMASVAQQLSIGMGVVLAGFALQMSNEIQGHATLVAQDFWPAFLVVGAVAA 489 D + TS+ASV QQLS+G+GV +AG LQ+SN +QGHAT+V DFWPAFLV+G + Sbjct: 361 DSRDAGRVTSLASVIQQLSLGLGVTVAGIVLQLSNHLQGHATIVEADFWPAFLVIGLFSV 420 Query: 490 CALLMLRQLPHDAGAEMA 507 ++ + +LPH+AG E++ Sbjct: 421 ASVPISGKLPHNAGLELS 438 >gi|300313188|ref|YP_003777280.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 498 Score = 342 bits (878), Expect = 4e-95, Method: Compositional matrix adjust. Identities = 195/462 (42%), Positives = 281/462 (60%), Gaps = 6/462 (1%) Query: 53 RTFIIPLIVACALFMEQMDSTVISTSLPALALDMNVDPISLKLALTSYLVSLAVFIPISG 112 R ++P IVA FME +D+T+I+T+LP +A V P L L +T+Y+++LAVFIPISG Sbjct: 28 RVRMLPYIVAATFFMEYLDTTIIATALPQMAQSFGVGPNELSLGMTAYMLTLAVFIPISG 87 Query: 113 WMADRFGSLTIFRSAICVFMLGSILCGVSSTLPQFVAARFLQGIGGAMMVPVGRLVILRS 172 W+ADRFGS T+F AI +F L SILCG S + +F AAR LQG+GGAMMVPVGR++++RS Sbjct: 88 WVADRFGSRTVFGMAIAIFTLASILCGFSQNVWEFTAARVLQGLGGAMMVPVGRMIVVRS 147 Query: 173 VEKVNLVKALSYLTIPALFGPVLGPPLGGFITTYFHWRWIFFINIPISILGLYLATRFIP 232 K L+KA++ +T PA+ PV+GP +GGFITTY W WIF +N+P + + Sbjct: 148 TGKDRLMKAIATITWPAIVAPVVGPSIGGFITTYASWHWIFLMNVPFGLAAFTAVMMVVR 207 Query: 233 DLKAEQVAPLDLKGFLLSALGCSLLMMGLATAGRHLVSGEVALTCVLAGLVFLAAYVWHA 292 + PLDL GFLLS + L+ G A + +VAL V G+V H Sbjct: 208 NEHGVSKRPLDLVGFLLSGAALTSLLYGTEIASHMESNIKVALGFVALGVVLGYGAWRHM 267 Query: 293 RRASAPLIDLALLKIPTLRAGVFGGSFYRVGIGAIPFLLPLMLQLGFGYTPFHSGMLTCA 352 +R PL+D + L+IPT V GS R+G+ A+P+L+PLM Q+GFG T F SG+L A Sbjct: 268 QRIEHPLLDFSNLRIPTYSVTVLTGSLTRIGVDAVPYLMPLMFQIGFGLTAFQSGLLLLA 327 Query: 353 SAAGAMFMKTIAARFLRRYGFRRVLLVNGALGVLGLWAIATFTPQTPYLLMVVILLIGGC 412 +A G + MK R L+R+GFR V + N A+ + A TP TP +++ ++ G Sbjct: 328 TALGNLGMKAFTTRILQRFGFRSVAVNNAAIAGGLIIACGLLTPATPLPVILGVIFCYGL 387 Query: 413 FRSLQFTSLNAISYADVDKLRLSQATSMASVAQQLSIGMGVVLAGFALQMSNEIQGHAT- 471 RS+QF++L ++YAD+ R S A+++ SVAQQ++IGMG+ AL+++ ++G A Sbjct: 388 SRSMQFSTLATLAYADISDARKSSASTLWSVAQQMTIGMGIAFGALALRIAAALRGEAID 447 Query: 472 -----LVAQDFWPAFLVVGAVAACALLMLRQLPHDAGAEMAG 508 DF AF + G V +++ R L DAG+ + G Sbjct: 448 ASGAHFTLGDFQWAFAMAGVVTLISVIGYRGLARDAGSNIGG 489 >gi|300312683|ref|YP_003776775.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 498 Score = 253 bits (645), Expect = 3e-68, Method: Compositional matrix adjust. Identities = 156/440 (35%), Positives = 243/440 (55%), Gaps = 5/440 (1%) Query: 60 IVACALFMEQMDSTVISTSLPALALDMNVDPISLKLALTSYLVSLAVFIPISGWMADRFG 119 IVA FM+ +D+T+++T+LPA+A + +++ + +Y +++A P SGW+ADRFG Sbjct: 57 IVASGFFMQTLDTTIVNTALPAMARSLGEPVLNMHPVVVAYTLTMASLTPASGWLADRFG 116 Query: 120 SLTIFRSAICVFMLGSILCGVSSTLPQFVAARFLQGIGGAMMVPVGRLVILRSVEKVNLV 179 + ++ +AI +F LGS+LC ++S+LP +AAR +QG+GG+M++PVGRL +LR+V + Sbjct: 117 TRRMYFAAILLFSLGSLLCAMASSLPMLLAARVVQGLGGSMLLPVGRLAVLRAVPGAQYI 176 Query: 180 KALSYLTIPALFGPVLGPPLGGFITTYFHWRWIFFINIPISILGLYLATRFIPDLKAEQV 239 AL+ ++I GPV+GP LGG++ W WIF IN+P+ +LG+ F+PD Sbjct: 177 AALASISIAGQLGPVVGPVLGGWLVESLSWHWIFLINLPVGVLGMGAVLYFLPDDSLAGT 236 Query: 240 APLDLKG-FLLSALGCSLLMMGLATAGRHLVSGEVALTCVLAGLVFLAAYVWHARRASAP 298 DL G LLSA + + H +AL V A V Y HA P Sbjct: 237 PRFDLLGCVLLSACMVAFSLALDVPFDSHRTGWSIALFVVAALTVL--GYWRHASVHPRP 294 Query: 299 LIDLALLKIPTLRAGVFGGSFYRVGIGAIPFLLPLMLQLGFGYTPFHSGMLTCASAAGAM 358 L L L P G+ G R+G A+PFLLPL+LQ+ GY+P H+GM+ +A Sbjct: 295 LFRLGLFGEPNFSIGLAGNLVCRIGSSAVPFLLPLLLQVRLGYSPLHAGMVLLPAAIAGT 354 Query: 359 FMKTIAARFLRRYGFRRVLLVNGALGVLGLWAIATFTPQTPYLLMVVILLIGGCFRSLQF 418 K A ++R+G+ R LLVN A+ + + A TP P +V L I G S+QF Sbjct: 355 IAKPWIAPLVKRFGYDRFLLVNTAIVGAAIMSFALITPDWPLTAAIVQLAIFGASNSMQF 414 Query: 419 TSLNAISYADVDKLRLSQATSMASVAQQLSIGMGVVLAGFALQMSNEIQGHATLVAQDFW 478 ++N+++ +DK S+ S+ Q L+IG+GV + G + + G AT F Sbjct: 415 AAMNSVTLKGLDKRDAGSGNSLFSMVQMLAIGLGVTIGGTMVNLFKSEVGTAT--NASFQ 472 Query: 479 PAFLVVGAVAACALLMLRQL 498 F +VGA+ + + R++ Sbjct: 473 ITFAIVGAITLASAWVFRKM 492 >gi|300312017|ref|YP_003776109.1| multidrug resistance B (translocase) transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 524 Score = 135 bits (340), Expect = 8e-33, Method: Compositional matrix adjust. Identities = 114/425 (26%), Positives = 202/425 (47%), Gaps = 15/425 (3%) Query: 59 LIVACALFMEQMDSTVISTSLPALALDMNVDPISLKLALTSYLVSLAVFIPISGWMADRF 118 L V+ A+FM +DS++ + ++P ++ ++ V +TS+ + A+ IP++GW+ R Sbjct: 27 LSVSLAVFMNVLDSSIANVAIPTISGNLGVSVDEGTWVITSFAAANAISIPLTGWLTQRL 86 Query: 119 GSLTIFRSAICVFMLGSILCGVSSTLPQFVAARFLQGIGGAMMVPVGRLVILRSVEKVNL 178 G++ +F +++ +F+L S LCG++ TLP +AAR LQG+ MVP+ + ++L + K Sbjct: 87 GAVRLFVTSVLLFVLASWLCGLAPTLPILLAARVLQGLVAGPMVPLSQSLLLGAYPKSKS 146 Query: 179 VKALSYLTIPALFGPVLGPPLGGFITTYFHWRWIFFINIPISIL--GLYLA---TRFIPD 233 AL+ + A+ PV GP LGG+IT + W WIF+INIP+ ++ G+ LA +R P Sbjct: 147 SFALALWGMTAVVAPVAGPALGGWITDSYTWPWIFYINIPVGLIAAGVTLAIYRSRETPT 206 Query: 234 LKAEQVAPLDLKG--FLLSALGCSLLMMGLATAGRHLVSGEVALTCVLAGLVFLAAYVWH 291 K P+D G L++ + +M+ S + ++A + F VW Sbjct: 207 HK----LPIDKIGLALLVTWVAAFQIMLDKGKDLDWFASPTIVTLGIIALIAFAYFVVWE 262 Query: 292 ARRASAPLIDLALLKIPTLRAGVFGGSF-YRVGIGAIPFLLPLMLQLGFGYTPFHSGMLT 350 P++DL L G S Y V G + +LP LQ GY SG+ T Sbjct: 263 LNN-DHPVVDLRLFGRRNFAGGTIAISIGYGVFFGNL-VILPQWLQQYLGYRSIDSGLST 320 Query: 351 CASAAGAMFMKTIAARFLRRYGFRRVLLVNGALGVLGLWAIATFTPQTPYLLMVVILLIG 410 A+ + + RFL R+V + + W + + Q + +++ L+ Sbjct: 321 APIGIFAVILMPLIGRFLPLVDARKVATASFIGFAIVFWLRSRYNLQVDQMTVILPTLLQ 380 Query: 411 GCFRSLQFTSLNAISYADVDKLRLSQATSMASVAQQLSIGMGVVLAGFALQMSNEIQGHA 470 G ++ F L + + + R+ A ++S + +G ++ A + I HA Sbjct: 381 GIPTAMFFVPLTVLLLSGLPPERIPAAAGLSSFVRVFCGAVGTSISTTAWN-NRSIMHHA 439 Query: 471 TLVAQ 475 L Sbjct: 440 QLTEH 444 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.329 0.142 0.430 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,451,535 Number of extensions: 56880 Number of successful extensions: 357 Number of sequences better than 1.0e-08: 17 Number of HSP's gapped: 337 Number of HSP's successfully gapped: 17 Length of query: 515 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 426 Effective length of database: 1,198,455 Effective search space: 510541830 Effective search space used: 510541830 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 133 (55.8 bits)